73 results about "Nitroreductase" patented technology

The invention belongs to the field of agricultural biotechnology and environmental microbiological engineering, and discloses mesotrione nitroreductase Mstnr as well as a coding gene and application thereof. The nucleotide sequence of the gene is SEQ ID NO.1, the full length is 678bp, 225 amino acids are encoded, and the amino acid sequence of the gene is SEQ ID NO.2. The highest homology of the Mtnr and reported nitroreductase is only 49.2%, so that the Mtnr is a new nitroreductase gene. The nitroreductase Mtnr provided by the invention can catalyze nitro reduction of mesotrione and convert mesotrione into a product 2-amino-4-methylsulfonylbenzoic acid (AMBA) without herbicidal activity, which indicates that the Mtnr gene has a huge application prospect in biodegradation detoxification and repair of mesotrione.

The invention belongs to the field of agricultural biotechnology and environmental microbiological engineering, and discloses mesotrione nitroreductase Mtnr as well as a coding gene and application thereof. The nucleotide sequence of the gene is SEQ ID NO.1, the full length is 756bp, 251 amino acids are encoded, and the amino acid sequence is SEQ ID NO.2. The maximum homology of the Mstnr and reported nitroreductase is only 50.6%, so that the Mstnr is a new nitroreductase gene. The nitroreductase Mstnr provided by the invention can catalyze nitro reduction of mesotrione, and the mesotrione is converted into a product 2-amino-4-methylsulfonyl benzoic acid (AMBA) without herbicidal activity, so that the Mstnr gene has a huge application prospect in biodegradation detoxification and repair of the mesotrione.

The invention belongs to the field of agricultural biotechnology and environmental microbiological engineering, and discloses a novel mesotrione nitroreductase Bamnr, and a coding gene and application thereof. The nucleotide sequence of the gene is SEQ ID NO.1, the full length is 639bp, 212 amino acids are encoded, and the amino acid sequence of the gene is SEQ ID NO.2. The highest homology of Bamnr and reported nitroreductase is only 69.4%, so that Bamnr is a new nitroreductase gene. The nitroreductase Bamnr provided by the invention can catalyze nitro reduction of mesotrione, and the mesotrione is converted into a product 2-amino-4-methylsulfonyl benzoic acid (AMBA) without herbicidal activity, so that the Bamnr gene has a huge application prospect in biodegradation detoxification and repair of the mesotrione.

The invention belongs to the field of agricultural biotechnology and environmental microbiological engineering, and discloses a herbicide mesotrione degradation detoxification enzyme protein, and a coding gene and application thereof. The nucleotide sequence of the gene is SEQ ID NO.1, the full length of the gene is 738bp, 245 amino acids are encoded, and the amino acid sequence of the gene is SEQ ID NO.2. The maximum homology of the Mdmnr and reported nitroreductase is only 48%, so that the Mdmnr is a new nitroreductase gene. The nitroreductase Mdmnr provided by the invention can catalyze nitro reduction of mesotrione, and the mesotrione is converted into a product AMBA without herbicidal activity, which indicates that the Mdmnr gene has huge application prospects in mesotrione-resistant transgenic engineering and biodegradation detoxification and repair of mesotrione residual pollution.

The invention discloses a nitroreductase response fluorescence and magnetic resonance bimodal probe NO2-Cy-Gxtb-Gd-Biotin designed on the basis of a gemcitabine prodrug as well as a synthesis method and application of the nitroreductase response fluorescence and magnetic resonance bimodal probe NO2-Cy-Gxtb-Gd-Biotin. The probe has relatively low fluorescence signals and magnetic resonance signals, and has relatively low biotoxicity. After the nitroreductase responds, on one hand, the gemcitabine anti-cancer drug is released, and tumor cell apoptosis is promoted; on the other hand, a molecular skeleton can generate a series of electron transfer to form an intermediate structure of methylquinone, and the intermediate structure of methylquinone is very high in reaction activity and is easily attacked by an external residue nucleophilic group of protein, so that the probe is anchored on nitroreductase, and a fluorescence signal and a magnetic resonance signal are remarkably enhanced; therefore, tumor enrichment is increased, an imaging time window is expanded, and early monitoring of tumors is facilitated. The medicine and the imaging probe are integrated, so that the treatment of monitoring tumors is facilitated.

The invention belongs to the field of environmental microorganisms and agriculture, and discloses mesotrione nitroreductase as well as a coding gene and application thereof. The amino acid sequence of the mesotrione nitroreductase MnrA is as shown in SEQ ID NO. 2; the nucleotide sequence of the gene for coding mesotrione nitroreductase MnrA is as shown in SEQ ID NO. 1; the invention also provides application of the mesotrione nitroreductase and the gene as well as an expression box, a recombinant vector and a genetically engineered bacterium capable of degrading mesotrione. The homology of the nitroreductase MnrA provided by the invention and reported nitroreductase is lower than 20%, and the nitroreductase MnrA is a novel nitroreductase. The nitroreductase MnrA disclosed by the invention can catalyze nitro reduction of mesotrione, and the mesotrione is converted into a product AMBA without herbicidal activity, so that the nitroreductase MnrA and the coding gene thereof have huge application prospects in mesotrione-resistant transgenic engineering and biodegradation detoxification and repair of mesotrione residual pollution.

A method for reducing a substrate selected from 2-methyl-5-nitropyridine and methyl 4-(2-fluoro-3-nitrobenzyl)piperazine-1-carboxylate is provided catalysed by a nitroreductase and a disproportionation agent.

This invention discloses a near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe, its preparation method, and its application, belonging to the field of medical detection technology. The structural formula of the near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe is as follows: The near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe of this invention possesses a hypoxia dual-mode visual monitoring molecular framework, enabling dual-mode determination of hypoxia microenvironments. It improves the stability of the cyanine near-infrared framework and achieves highly stable and specific detection of nitroreductase under hypoxia. Under hypoxia conditions, the product reacts with NTR, and the single-photon excited near-infrared fluorescence is quenched and then illuminated. Simultaneously, under hypoxia, the probe reacts with Cys to generate a two-photon excited fluorescence signal, effectively avoiding the probe consumption problem of dual-response systems and ensuring the non-interference of fluorescence excitation and emission (dual-mode), thus truly achieving accurate locking of hypoxia microenvironments.

The invention belongs to the technical field of microorganisms, and particularly relates to an iaam gene overexpression pseudomonas nitroreductase engineering bacterium as well as a construction method and application of the iaam gene overexpression pseudomonas nitroreductase engineering bacterium. The construction method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification on an iaam gene in genomic DNA (Deoxyribose Nucleic Acid) of the pseudomonas nitroreducens LSF-6 by taking genomic DNA of the pseudomonas nitroreducens LSF-6 as a template; a PCR amplification product is connected to a pBBR1MCS vector, and an iaam gene overexpression vector is constructed; and transforming the iaam gene overexpression vector into the nitroreductive pseudomonas LSF-6 to obtain the nitroreductive pseudomonas engineering bacteria for overexpressing the iaam gene. Compared with the original strain, the auxin secretion capability of the pseudomonas nitroreducens engineering strain is obviously improved. When the pseudomonas nitroreducens engineering bacteria are prepared into bacterial suspension to be applied to rice, the plant height, the root length, the overground part fresh weight, the underground part fresh weight and the chlorophyll SPAD value of the rice can be obviously increased. The invention lays a foundation for promoting rice growth by a microbiological method.

The invention discloses a xanthene nitroreductase-responsive fluorescent dye compound as well as a preparation method and application thereof, and belongs to the technical field of xanthene derivatives. The fluorescent dye compound can be used for quickly and specifically recognizing overexpressed nitroreductase in tumor cells; after the fluorescent probe acts with nitroreductase, the fluorescence intensity of the fluorescent probe at 718 nm is obviously enhanced, and the fluorescent probe has excellent targeting capability on mitochondria; meanwhile, under the excitation of illumination with the specific wavelength of 660 nm, an acting product of the fluorescent dye compound and nitroreductase can efficiently generate reactive oxygen species (ROS), and then the effect of specifically killing tumor cells is achieved. The xanthene nitroreductase response type fluorescent dye compound provided by the invention can achieve the goal of integration of diagnosis and treatment of solid tumors, and has important clinical application value.

The invention provides a nitroreductase mutant as well as a preparation method and application thereof. The nitroreductase mutant comprises at least one amino acid mutation in the following seven sites or functional equivalent sites compared with a wild type nitroreductase: the 40th site, the 103rd site, the 201st site, the 231st site, the 256th site, the 258th site and the 267th site; wherein the wild type nitroreductase has an amino acid sequence as shown in SEQ ID NO: 1. The nitroreductase mutant has higher catalytic activity and stability, and can obviously improve the catalytic efficiency, save the production cost and reduce the environmental pollution in industrial application.

The present invention belongs to the field of medicinal chemistry technology and relates to a lysosomal-targeted nitroreductase-responsive fluorescent probe based on a triphenylamine backbone, its synthesis method, and its application. The triphenylamine derivative-based lysosomal-targeted nitroreductase-responsive fluorescent probe provided by the present invention does not emit light upon initial entry into cells. However, after a period of culture in cancer cells, the electron-withdrawing nitro group is reduced by the intracellular nitroreductase. Under laser irradiation, the charge within the fluorophore shifts, resulting in fluorescence. The present invention also provides a method for synthesizing the lysosomal-targeted nitroreductase-responsive fluorescent probe and its use in bioimaging.

Host-guest supramolecular probes and applications for accelerating nitroreductase detection belong to the fields of organometallic supramolecular, bioinorganic and biofluorescent probes. The supramolecular host-guest probe platform, which is assembled based on the metal organic cage main structure constructed based on the cofactor structure and the nitroreductase small molecule fluorescent probe, can be used for the rapid quantitative detection of nitroreductase in tumors, simplifying the enzyme-catalyzed double substrate process to a single substrate process. The traditional fluorescent sensor that depends on the cofactor concentration is no longer interfered by the cofactor, and has a rapid fluorescent signal response to the detection of the target enzyme. There is a linear relationship between the fluorescence emission change of the host-guest probe and the nitroreductase concentration, and compared with the simple small molecule probe, the host-guest probe shows better sensitivity to the target enzyme. This host-guest supramolecular fusion strategy provides an effective tool for the new rapid quantitative detection of nitroreductase and provides a new method for biological tracing.

The invention relates to synthesis and anti-tumor application of a nitroreductase activated camptothecin chemiluminescent prodrug. The prodrug molecule (CPT-NBz-CL) integrates three key functional units through a 2, 6-hydroxymethyl p-cresol derived self-burning group: (i) a nitrobenzene group (NBz) as a nitroreductase (NTR) specific trigger; (ii) a potent chemotherapeutic drug camptothecin (CPT) as a cytotoxic load; (iii) a Schaap type phenolic group-dioxetane structure is used as a chemiluminescence reporter group (CL), and the structure is shown as a formula (I). In order to carry out comparative research, a control molecule CL-NBz-CL which lacks a CPT load but retains a trigger and a reporter group is synchronously synthesized. The structures and optical properties of the two molecules are comprehensively characterized through analysis means such as nuclear magnetic resonance hydrogen spectrum, carbon spectrum, absorption spectrum, fluorescence spectrum and luminescent spectrum, and the molecules can verify that the molecules have specific detection capability on NTR through chemiluminescence, fluorescence spectrum, selectivity and the like in vitro.

The present disclosure relates to compositions comprising nitroreductase enzymes; plants, plant calluses, plant seeds, and vegetables incorporating genes expressing nitroreductase enzymes; methods for stably and operably incorporating genes expressing nitroreductase enzymes into plants and plant tissues; wherein the nitroreductase enzymes are capable of reducing nitro groups on phytotoxic and / or otherwise harmful compounds such as, for example, thaxtomins, wherein the thaxtomins are secreted by plant pathogenic bacteria and / or exogenously applied as an agricultural composition such as an herbicide, and wherein reducing the nitro groups renders the thaxtomins non-damaging or reduces the level of damage from the thaxtomins to the plants expressing the nitroreductase enzymes.

This invention belongs to the field of fluorescent probe technology, specifically relating to a nitroreductase fluorescent probe, its preparation method, and its application. To develop an NTR fluorescent probe with high selectivity, high sensitivity, short response time, and long excitation and emission wavelengths, this invention utilizes pentamethine cyanine dye and a "ester group → carboxyl group" conversion strategy at the central site of this dye to develop a nitroreductase fluorescent probe, Cy5-NTR. In the presence of NADPH, the nitro group of the probe is first catalyzed by the overexpression of NTR by cancer cells, resulting in a single-electron transfer and the generation of a nitro anion radical. This radical is then further reduced to an amino group, followed by a 1,6-rearrangement and elimination reaction, releasing the strongly fluorescent centrally carboxyl-substituted pentamethine cyanine dye Cy5-COO. This probe can not only distinguish cancer cells / tissues from normal cells / tissues with high contrast under hypoxic conditions, but also provide in situ real-time imaging of tumors in tumor-bearing mice.

The application belongs to the field of agricultural biotechnology and environmental microbial engineering, and discloses a mesotrione nitroreductase Mtnr, a coding gene thereof and application. The nucleotide sequence is SEQ ID NO. 1, the full length is 756 bp, and 251 amino acids are encoded, and the amino acid sequence is SEQ ID NO. 2. The highest homology of Mtnr with a reported nitroreductase is only 50.6%, and therefore it is a new nitroreductase gene. The nitroreductase Mtnr provided by the application can catalyze the nitroreduction of mesotrione, and convert mesotrione into a product 2-amino-4-methylsulfonylbenzoic acid (AMBA) without herbicidal activity, indicating that the Mtnr gene has great application prospect in mesotrione biodegradation detoxification and remediation.

The invention discloses a mesotrione nitroreductase gene xhctnr, a protein coded by the mesotrione nitroreductase gene xhctnr and application of the mesotrione nitroreductase gene xhctnr The nucleotide sequence of the mesotrione nitroreductase gene xhctnr disclosed by the invention is as shown in SEQ ID NO.1, the encoded protein Xhctnr is mesotrione nitroreductase Xhctnr, and the amino acid sequence of the mesotrione nitroreductase gene xhctnr is as shown in SEQ ID NO.2. The mesotrione nitroreductase Xhctnr can be used for degrading mesotrione into a product AMBA, so that the herbicidal activity is lost. The mesotrione nitroreductase gene xhctnr and the protein coded by the mesotrione nitroreductase gene xhctnr have a huge application prospect in biodegradation detoxification and bioremediation of mesotrione.

The invention belongs to the technical field of medicine preparation, and particularly relates to a ferroptosis agonist, an anoxia response type nanometer co-delivery system and application thereof. The novel ferroptosis agonist RSL3-ClAc is synthesized by introducing a chloracetyl fragment into an RSL3 molecule, the binding capacity of the novel ferroptosis agonist RSL3-ClAc with GPX4 is remarkably enhanced, and then the ferroptosis induction efficiency is improved. And meanwhile, the RSL3-ClAc and the hypoxia response type photosensitizer TCy5-NO2 are subjected to self-assembly, so that the nano drug delivery system is constructed. According to the nano drug delivery system, the EPR effect and the hypoxia response characteristic of the nano carrier are utilized, the tumor barrier can be effectively broken through, and the focus can be accurately positioned. In a tumor hypoxic microenvironment, the activation of a Try5-NO2 photosensitizer is triggered by nitroreductase (NTR), and RSL3-ClAc is synchronously released to enhance the ferroptosis induction effect.

The invention relates to a preparation method of a vortioxetine intermediate, and particularly provides a method for reducing a compound shown as a formula (I) into a compound shown as a formula (II) by using nitroreductase. Wherein the nucleotide sequence of the nitroreductase is as shown in SEQ ID NO. 1. The preparation method provided by the invention has the advantages of mild reaction conditions, high safety, no use of a heavy metal catalyst, environmental protection and low cost, and is suitable for industrial production. # imgabs0 #

This invention relates to the field of microbial technology, disclosing a *Strombus adhesiveus* strain YF2 that degrades polyethylene mulch film, microbial preparations, methods, and applications, addressing the problems of insufficient efficacy and unclear mechanisms in existing PE-degrading strains. This strain is deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC No:67086, and a deposit date of October 13, 2025. Through systematic parallel comparisons, strain YF2 significantly outperformed the control strain in PE degradation efficiency, biofilm formation ability, and induction of PE polymer oxidation. Genomic analysis showed that YF2 contains a genetic network encoding key degrading enzyme systems such as nitroreductase, cytochrome P450, and alkane monooxygenase, and particularly possesses unique nitrification degradation potential. Microbial preparations prepared based on this strain can be used for the bioremediation of PE mulch film residues in farmland soils, compost, and other environments.

The present application relates to a kind of based on heptamethine cyanine molecule, its preparation method and application, belong to medical technology field.Compounds B1-3, C1 and C3 can specifically examine nitroreductase, be activated by nitroreductase and photoacoustic signal is enhanced.Introducing heavy atom, significantly improve photo-thermal conversion efficiency and intersystem crossing, promote photothermal therapy and photodynamic therapy.Compound C1 shows specific tumor killing effect and obvious photoacoustic signal in vivo by excellent synergistic PTT / PDT effect, provides a general strategy for cancer photoacoustic diagnosis and enhanced phototherapy.Compound C3 realizes staphylococcus aureus in situ pneumonia photoacoustic imaging for the first time, and can detect bacteria in tumor with high sensitivity and specificity, provides a promising new method for early detection of deep bacterial infection in vivo.

The invention discloses a preparation method and application of a pH / nitroreductase double-response type fluorescent probe, and belongs to the technical field of fluorescence detection. The probe is specifically activated only when the probe and nitroreductase coexist in an acidic microenvironment (pH = 6-7), fluorescence Turn-On response is achieved, the emission wavelength of the probe is located at 760-850 nm (near-infrared window), and the fluorescence intensity is enhanced by 13-132 times after the probe is activated. The probe has the advantages of high specificity, near-infrared quick response (the fluorescence is the strongest within 0-30 minutes after double conditions are met at the same time) and high sensitivity. In addition, the unique double-response mechanism effectively avoids the interference of a single factor, and the detection specificity and the signal-to-noise ratio in a complex biological system are remarkably improved.

The invention belongs to the technical field of microorganisms, and provides a gene combination for degrading organic arsenic, an engineering bacterium and application of the gene combination. The gene combination comprises a nitroreductase gene BsntrA, an arsenate reductase gene ArsC2 and an efflux pump gene BsexpA which are from brevundimonas M20, the gene combination is used for constructing a recombinant expression vector and transforming the recombinant expression vector into a host cell, the obtained gene engineering cell forms an organic arsenic reduction-secretion system through protein expressed by the gene, nitro reduction, arsenic (V) reduction and HAPA (III) secretion of roxarsone are achieved, the degradation rate of the gene engineering cell to roxarsone in wastewater reaches 75.6%, and the gene engineering cell has a good application prospect. The drug resistance to roxarsone is obviously improved, and the compound can be efficiently used for removing organic arsenic in pollutants.

The invention provides a dual-response fluorescent probe. The fluorescent probe has dual response on tumor microenvironment viscosity and nitroreductase. The synthesis raw materials of the probe compound are easy to obtain, the synthesis operation is simple, and the preparation is convenient; and the fluorescent probe has strong specificity and high sensitivity for tumor recognition, and is safe and non-toxic.

The present invention relates to the technical field of fluorescent probes, and in particular to a fluorescent probe for rapid detection of nitroreductase, a preparation method thereof, and an application thereof. The present invention provides a fluorescent probe for rapid detection of nitroreductase, a preparation method thereof, and an application thereof. The fluorescent probe is named DM-CN. Due to the quenching effect of the nitro group, DM-CN is almost non-fluorescent. However, when DM-CN reacts with NTR in the presence of NADH, the 4-nitrobenzene substrate is reduced to the corresponding amine by NTR, the ether bond is broken, and then the phenol oxygen undergoes an intramolecular nucleophilic attack on the nitrile group to form iminocoumarin, and then the fluorescence emission is significantly enhanced, thereby achieving specific recognition of nitroreductase. The fluorescent probe DM-CN has the characteristics of high specificity, high sensitivity, high fluorescence intensity response, short response time, and little damage to organisms, and can be used for the detection of nitroreductase in A549 cells and zebrafish.

The invention relates to the technical field of fluorescent probes, and discloses a pH-NTR cascade response type nanoprobe, a preparation method and application, the nanoprobe is formed by encapsulating an NTR response type fluorescent probe CyBrN by a liposome self-assembled by a pH response type molecule DCPA, and the nanoprobe can be subjected to structural dissociation in a tumor acidic microenvironment and realize controllable release of probe molecules, so that the pH-NTR cascade response type nanoprobe can be used for detecting tumor tumors. The released probe can be specifically recognized by overexpressed nitroreductase in tumor cells and is subjected to reduction activation, so that a near-infrared fluorescence signal is recovered, and accurate positioning of the tumor cells is realized; meanwhile, the released probe efficiently generates active oxygen under laser irradiation, precise photodynamic killing of tumor cells is achieved, and the probe can be used as a PDT photosensitizer.

The invention provides a nitroreductase mutant and application thereof in preparation of p-anisidine, and relates to the technical field of biology. The nitroreductase mutant provided by the invention is obtained by mutating wild type nitroreductase with an amino acid sequence as shown in SEQ ID NO.1, and the mutation comprises one of the following mutations: arginine at the 224th site is mutated into glycine; asparagine at the 43rd site is mutated into threonine; the alanine at the 218th site is mutated into tryptophan; glutamic acid at the 166th site is mutated into lysine. Compared with wild type nitroreductase, the nitroreductase mutant is higher in enzyme activity, p-nitroanisole can be efficiently catalyzed to generate p-aminoanisole under the synergistic catalysis of V2O5, the nitroreductase mutant is further used for preparing alzapine, and meanwhile, under the system, the substrate spectrum of nitroreductase catalysis is expanded.

This invention provides a nitro-aryl ring-substituted imidazopyrazine compound that can be used as a bioluminescent probe for detecting nitroreductases. In the NanoLuc luciferase bioluminescent system, this probe exhibits good responsiveness in in vitro experiments and can be selectively reduced by NTRs. Viable bacterial experiments and imaging experiments in a mouse model of bacterial infection both demonstrate that this probe is a highly selective and sensitive luminescent probe for detecting NTR activity, enabling the detection and imaging of NTR activity in vivo.

A biosensor for measuring nitroreductase, a preparation method for a biosensor, and a quantitative measurement method for nitroreductase. The preparation method for the biosensor comprises the following steps: uniformly mixing an ethanol solution of Cy-No2 and a hydrochloric acid solution of Fmoc-H, then adding a Zn2+ aqueous solution, uniformly stirring, adjusting the pH value of a system to 7, continuing stirring to react for 55-65 min, and then centrifuging, washing, and drying to prepare the biosensor for measuring nitroreductase. The preparation method is simple, and the biosensor can be prepared by simple one-step self-assembly, can be used for quantitative measurement of nitroreductase, and can be used for monitoring the nitroreductase of hypoxic cancer cells by means of an in-vitro fluorescence biological imaging technology.