22 results about "Nitroreductase" patented technology

The invention discloses a xanthene nitroreductase-responsive fluorescent dye compound as well as a preparation method and application thereof, and belongs to the technical field of xanthene derivatives. The fluorescent dye compound can be used for quickly and specifically recognizing overexpressed nitroreductase in tumor cells; after the fluorescent probe acts with nitroreductase, the fluorescence intensity of the fluorescent probe at 718 nm is obviously enhanced, and the fluorescent probe has excellent targeting capability on mitochondria; meanwhile, under the excitation of illumination with the specific wavelength of 660 nm, an acting product of the fluorescent dye compound and nitroreductase can efficiently generate reactive oxygen species (ROS), and then the effect of specifically killing tumor cells is achieved. The xanthene nitroreductase response type fluorescent dye compound provided by the invention can achieve the goal of integration of diagnosis and treatment of solid tumors, and has important clinical application value.

This invention belongs to the field of fluorescent probe technology, specifically relating to a nitroreductase fluorescent probe, its preparation method, and its application. To develop an NTR fluorescent probe with high selectivity, high sensitivity, short response time, and long excitation and emission wavelengths, this invention utilizes pentamethine cyanine dye and a "ester group → carboxyl group" conversion strategy at the central site of this dye to develop a nitroreductase fluorescent probe, Cy5-NTR. In the presence of NADPH, the nitro group of the probe is first catalyzed by the overexpression of NTR by cancer cells, resulting in a single-electron transfer and the generation of a nitro anion radical. This radical is then further reduced to an amino group, followed by a 1,6-rearrangement and elimination reaction, releasing the strongly fluorescent centrally carboxyl-substituted pentamethine cyanine dye Cy5-COO. This probe can not only distinguish cancer cells / tissues from normal cells / tissues with high contrast under hypoxic conditions, but also provide in situ real-time imaging of tumors in tumor-bearing mice.

This invention relates to the field of microbial technology, disclosing a *Strombus adhesiveus* strain YF2 that degrades polyethylene mulch film, microbial preparations, methods, and applications, addressing the problems of insufficient efficacy and unclear mechanisms in existing PE-degrading strains. This strain is deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC No:67086, and a deposit date of October 13, 2025. Through systematic parallel comparisons, strain YF2 significantly outperformed the control strain in PE degradation efficiency, biofilm formation ability, and induction of PE polymer oxidation. Genomic analysis showed that YF2 contains a genetic network encoding key degrading enzyme systems such as nitroreductase, cytochrome P450, and alkane monooxygenase, and particularly possesses unique nitrification degradation potential. Microbial preparations prepared based on this strain can be used for the bioremediation of PE mulch film residues in farmland soils, compost, and other environments.

The invention discloses a preparation method and application of a pH / nitroreductase double-response type fluorescent probe, and belongs to the technical field of fluorescence detection. The probe is specifically activated only when the probe and nitroreductase coexist in an acidic microenvironment (pH = 6-7), fluorescence Turn-On response is achieved, the emission wavelength of the probe is located at 760-850 nm (near-infrared window), and the fluorescence intensity is enhanced by 13-132 times after the probe is activated. The probe has the advantages of high specificity, near-infrared quick response (the fluorescence is the strongest within 0-30 minutes after double conditions are met at the same time) and high sensitivity. In addition, the unique double-response mechanism effectively avoids the interference of a single factor, and the detection specificity and the signal-to-noise ratio in a complex biological system are remarkably improved.

The invention belongs to the technical field of microorganisms, and provides a gene combination for degrading organic arsenic, an engineering bacterium and application of the gene combination. The gene combination comprises a nitroreductase gene BsntrA, an arsenate reductase gene ArsC2 and an efflux pump gene BsexpA which are from brevundimonas M20, the gene combination is used for constructing a recombinant expression vector and transforming the recombinant expression vector into a host cell, the obtained gene engineering cell forms an organic arsenic reduction-secretion system through protein expressed by the gene, nitro reduction, arsenic (V) reduction and HAPA (III) secretion of roxarsone are achieved, the degradation rate of the gene engineering cell to roxarsone in wastewater reaches 75.6%, and the gene engineering cell has a good application prospect. The drug resistance to roxarsone is obviously improved, and the compound can be efficiently used for removing organic arsenic in pollutants.

The invention provides a dual-response fluorescent probe. The fluorescent probe has dual response on tumor microenvironment viscosity and nitroreductase. The synthesis raw materials of the probe compound are easy to obtain, the synthesis operation is simple, and the preparation is convenient; and the fluorescent probe has strong specificity and high sensitivity for tumor recognition, and is safe and non-toxic.

The invention relates to the technical field of fluorescent probes, and discloses a pH-NTR cascade response type nanoprobe, a preparation method and application, the nanoprobe is formed by encapsulating an NTR response type fluorescent probe CyBrN by a liposome self-assembled by a pH response type molecule DCPA, and the nanoprobe can be subjected to structural dissociation in a tumor acidic microenvironment and realize controllable release of probe molecules, so that the pH-NTR cascade response type nanoprobe can be used for detecting tumor tumors. The released probe can be specifically recognized by overexpressed nitroreductase in tumor cells and is subjected to reduction activation, so that a near-infrared fluorescence signal is recovered, and accurate positioning of the tumor cells is realized; meanwhile, the released probe efficiently generates active oxygen under laser irradiation, precise photodynamic killing of tumor cells is achieved, and the probe can be used as a PDT photosensitizer.

The invention provides a nitroreductase mutant and application thereof in preparation of p-anisidine, and relates to the technical field of biology. The nitroreductase mutant provided by the invention is obtained by mutating wild type nitroreductase with an amino acid sequence as shown in SEQ ID NO.1, and the mutation comprises one of the following mutations: arginine at the 224th site is mutated into glycine; asparagine at the 43rd site is mutated into threonine; the alanine at the 218th site is mutated into tryptophan; glutamic acid at the 166th site is mutated into lysine. Compared with wild type nitroreductase, the nitroreductase mutant is higher in enzyme activity, p-nitroanisole can be efficiently catalyzed to generate p-aminoanisole under the synergistic catalysis of V2O5, the nitroreductase mutant is further used for preparing alzapine, and meanwhile, under the system, the substrate spectrum of nitroreductase catalysis is expanded.

The invention relates to the technical field of chemiluminiscence detection, in particular to a rapid detection reagent in a chemiluminiscence mode and a preparation method of the rapid detection reagent, and the rapid detection reagent is characterized in that a core component of the rapid detection reagent is a nitroreductase-responsive flash type end product 5 as shown in a formula I, and the concentration is 0.1 mu M; the auxiliary reagent comprises reduced nicotinamide adenosine dinucleotide (NADH) and a Tris-HCl buffer solution (pH is 7.4), and the concentration of the reduced nicotinamide adenosine dinucleotide is 5 mu M. The reagent is used for detecting nitroreductase (NTR), a high-intensity chemiluminescence signal can be generated within 1 minute after the reagent acts with the NTR at 37 DEG C, the signal duration is larger than or equal to 40 minutes, and the NTR detection limit reaches 0.951 ng / mL. The problems that an existing flash type probe is complex in synthesis process, short in signal duration and insufficient in low-concentration NTR detection capacity are solved.

The present invention relates to a dual fluorescence probe for detecting nitroreductase and hydrogen sulfide, and a manufacturing method therefor. The dual fluorescence probe according to the present invention can detect both nitroreductase and hydrogen sulfide, which are indicators of a hypoxic environment, and can provide low background fluorescence noise and selective, high fluorescence signals, thus enabling normal cells and tumor cells in a hypoxic microenvironment to be accurately distinguished and detected through improved hypoxia detection effects and imaging capabilities.

The application belongs to the technical field of medicine, and discloses a fluorescent probe for detecting nitroreductase (NTR) activity, a preparation method and use thereof. The probe is constructed by coupling p-nitrobenzyl bromide and a hemicyanine fluorescent group, can specifically release the fluorescent group under the action of NTR, and produce a significant fluorescent signal. The probe is sensitive in response in a biological system, has excellent biocompatibility, and shows good application potential in the fields of tumor diagnosis and biological imaging.

This invention provides a two-photon fluorescent probe for detecting hypoxia levels. The probe comprises a core fluorophore composed of a 2-dimethylamino-7-hydroxynaphthalene two-photon fluorophore and a probe composed of a sulfonate methylene group. This invention also provides a method for preparing and applying the two-photon fluorescent probe for detecting hypoxia levels. The fluorescent probe of this invention enables the monitoring of hypoxia levels in tumor and normal tissues by in-situ detection of nitroreductase (NTR) content within tissues. It features fast response, high sensitivity, high optical stability, good biocompatibility, high selectivity, and high depth, high resolution, and low biological background in two-photon imaging. In cell imaging applications, this probe exhibits good cell permeability and can detect NTR content at the cellular level, indicating cellular hypoxia.

Provided is a compound comprising the structure:(SIG)-(SI-MOD)m.In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.

The application discloses a near-infrared fluorescence and photoacoustic dual-mode small molecule probe activated by nitroreductase, which can be activated by nitroreductase to be converted into a compound with fluorescence and photoacoustic signals, and a compound structure general formula of the small molecule probe is shown in the following formula: wherein R can be substituted at 4, 5, 6 and 7 positions, and the substituent group is one of H, OCH3, F and Cl; the small molecule probe compound itself has no fluorescence and photoacoustic; under catalysis of nitroreductase, the nitro group is reduced to an amino group to be converted into a compound integrating optical imaging and photoacoustic imaging, therefore, the small molecule probe is applied to tumor hypoxia imaging, near-infrared fluorescence and photoacoustic dual-mode can be simultaneously started only by activation of nitroreductase in tumor cells, and high specificity, high resolution, high sensitivity and high penetration depth imaging of the tumor can be realized.

The invention provides a compound with a cyanine structure and a preparation method and application thereof, the compound has a structure as shown in formula I. The compound with the cyanine structure has the characteristic of performing double recognition on nitroreductase (NTR) highly expressed in a tumor anoxic microenvironment and a tumor acidic microenvironment, and can be used as a fluorescent probe for performing specific recognition on tumors; the accuracy and precision of tumor recognition are improved, and the false positive rate in the tumor recognition process is reduced.

The invention discloses a novel mesotrione nitroreductase Wmnrs, a coding gene wmnrs of the novel mesotrione nitroreductase Wmnrs and application of the novel mesotrione nitroreductase Wmnrs and the coding gene wmnrs. The novel mesotrione nitroreductase Wmnrs can degrade mesotrione into 2-amino-4-methylsulfonyl benzoic acid (AMBA) to lose activity. After the gene is introduced into a plant, the plant with mesotrione-resistant transgene can be cultivated. The mesotrione nitroreductase Wmnrs and the coding gene of the mesotrione nitroreductase Wmnrs have potential application value in bioremediation of mesotrione residue pollution.

This invention relates to a BsNTR mutant nitroreductase and its applications, belonging to the field of biotechnology. The BsNTR mutant is BsNTR-M, and its amino acid sequence is shown in SEQ ID NO.2. This invention designs and modifies wild-type BsNTR-WT from Bacillus subtilis. The resulting mutant BsNTR-M not only exhibits significantly improved thermostability (Tm value 14°C higher than wild-type BsNTR-WT) but also demonstrates 1.6 times enhanced enzyme catalytic activity. This effectively overcomes the technical bottlenecks of poor thermostability, weak environmental tolerance, and limited activity inherent in wild-type enzymes. Furthermore, it possesses excellent expression and purification performance, allowing for efficient expression and purification in prokaryotic expression systems while retaining high specificity for nitro compounds and good biocompatibility and environmental safety. Large-scale production can be achieved without complex equipment.

This invention discloses a dual-response fluorescent probe for detecting nitroreductase and enhanced fluorescence signal under high viscosity conditions, its preparation method, and its application. This dual-response fluorescent probe selectively reacts with nitroreductase and produces 582 nm orange-red fluorescence emission, exhibiting both high selectivity and high sensitivity under high viscosity conditions. The dual-response fluorescent probe for detecting nitroreductase and enhanced fluorescence signal under high viscosity conditions of this invention has the structure shown in Formula I, and is named: (E)-2-(5-methyl-2-((4-nitrobenzyl)oxy)-3-(2-(quinolin-2-yl)vinyl)phenyl)benzo[d]thiazole, abbreviated as: HBT-QN-2 or 2-(2-hydroxyphenyl)benzothiazole derivative; I.

This invention relates to the fields of biopharmaceutical and biochemical technologies, specifically to a one-pot, two-enzyme method for the highly enantioselective reduction of chiral morpholine compounds. Employing nitroreductase NfsA and imine reductase IR-14 in synergistic action with an NADPH coenzyme system, the highly efficient biocatalytic transformation of chiral morpholine compounds was successfully achieved. This technology exhibits superior catalytic efficiency, with an overall substrate conversion rate exceeding 60%; absolute stereoselectivity, with an enantiomeric excess (ee%) of over 90%; and a simple reaction system operating under mild conditions (ambient temperature and pressure), meeting the requirements of green chemistry. Compared to traditional chemical synthesis methods, the biocatalytic pathway of this invention demonstrates significant advantages in atom economy, environmental friendliness, and product optical purity, providing an innovative solution for the synthesis of chiral heterocyclic drugs containing N and O.

The application belongs to the field of agricultural biotechnology and environmental microbial engineering, discloses a new mesotrione nitroreductase Bamnr and a coding gene and use thereof. The nucleotide sequence is SEQ ID NO. 1, the full length is 639 bp, and 212 amino acids are encoded, and the amino acid sequence is SEQ ID NO. 2. The highest homology of Bamnr with a reported nitroreductase is only 69.4%, and therefore it is a new nitroreductase gene. The nitroreductase Bamnr provided by the application can catalyze the nitroreduction of mesotrione, and convert mesotrione into a product 2-amino-4-methylsulfonylbenzoic acid (AMBA) without herbicidal activity, indicating that the Bamnr gene has great application prospect in mesotrione biodegradation detoxification and remediation.