6 results about "Nitroreductase" patented technology

This invention relates to the field of microbial technology, disclosing a *Strombus adhesiveus* strain YF2 that degrades polyethylene mulch film, microbial preparations, methods, and applications, addressing the problems of insufficient efficacy and unclear mechanisms in existing PE-degrading strains. This strain is deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC No:67086, and a deposit date of October 13, 2025. Through systematic parallel comparisons, strain YF2 significantly outperformed the control strain in PE degradation efficiency, biofilm formation ability, and induction of PE polymer oxidation. Genomic analysis showed that YF2 contains a genetic network encoding key degrading enzyme systems such as nitroreductase, cytochrome P450, and alkane monooxygenase, and particularly possesses unique nitrification degradation potential. Microbial preparations prepared based on this strain can be used for the bioremediation of PE mulch film residues in farmland soils, compost, and other environments.

The application discloses application of a nitroreductase inhibitor in preparation of a drug for treating prostate cancer, and relates to the technical field of tumor treatment. The application provides application of the nitroreductase inhibitor in preparation of a drug for preventing and / or treating prostate cancer in combination with flutamide, the nitroreductase inhibitor can inhibit metabolism of flutamide, and elimination of escherichia coli can improve the antitumor effect of flutamide.

The application belongs to the technical field of medicine, and discloses a fluorescent probe for detecting nitroreductase (NTR) activity, a preparation method and use thereof. The probe is constructed by coupling p-nitrobenzyl bromide and a hemicyanine fluorescent group, can specifically release the fluorescent group under the action of NTR, and produce a significant fluorescent signal. The probe is sensitive in response in a biological system, has excellent biocompatibility, and shows good application potential in the fields of tumor diagnosis and biological imaging.

The application discloses a Bacillus nitroreducens and application thereof in degradation of polycyclic nitrogen heterocyclic compounds. The application overcomes the double technical bottlenecks of stable structure and difficult degradation of polycyclic nitrogen heterocyclic compounds and sharp decline of degradation efficiency under anoxic conditions, and provides a Bacillus nitroreducens FL022 which can efficiently degrade nitrogen heterocyclic compounds, especially polycyclic nitrogen heterocyclic compounds under anoxic conditions. It is verified that the degradation rate of the Bacillus nitroreducens FL022 to typical difficult-to-degrade polycyclic nitrogen heterocyclic compounds such as benzocarbazole and naphthoimidazole can reach more than 88%, and the Bacillus nitroreducens FL022 has excellent environmental tolerance and stress resistance, and can maintain the degradation activity under high-concentration pollutant stress, and can be used in industrial wastewater treatment of multiple industries, and provides efficient, economic and environment-friendly microbial resources and solutions for solving the treatment problem of the high-toxicity and difficult-to-degrade pollutants.

This invention relates to a BsNTR mutant nitroreductase and its applications, belonging to the field of biotechnology. The BsNTR mutant is BsNTR-M, and its amino acid sequence is shown in SEQ ID NO.2. This invention designs and modifies wild-type BsNTR-WT from Bacillus subtilis. The resulting mutant BsNTR-M not only exhibits significantly improved thermostability (Tm value 14°C higher than wild-type BsNTR-WT) but also demonstrates 1.6 times enhanced enzyme catalytic activity. This effectively overcomes the technical bottlenecks of poor thermostability, weak environmental tolerance, and limited activity inherent in wild-type enzymes. Furthermore, it possesses excellent expression and purification performance, allowing for efficient expression and purification in prokaryotic expression systems while retaining high specificity for nitro compounds and good biocompatibility and environmental safety. Large-scale production can be achieved without complex equipment.

This invention discloses a dual-response fluorescent probe for detecting nitroreductase and enhanced fluorescence signal under high viscosity conditions, its preparation method, and its application. This dual-response fluorescent probe selectively reacts with nitroreductase and produces 582 nm orange-red fluorescence emission, exhibiting both high selectivity and high sensitivity under high viscosity conditions. The dual-response fluorescent probe for detecting nitroreductase and enhanced fluorescence signal under high viscosity conditions of this invention has the structure shown in Formula I, and is named: (E)-2-(5-methyl-2-((4-nitrobenzyl)oxy)-3-(2-(quinolin-2-yl)vinyl)phenyl)benzo[d]thiazole, abbreviated as: HBT-QN-2 or 2-(2-hydroxyphenyl)benzothiazole derivative; I.