42 results about "Nitroreductase" patented technology

A method for reducing a substrate selected from 2-methyl-5-nitropyridine and methyl 4-(2-fluoro-3-nitrobenzyl)piperazine-1-carboxylate is provided catalysed by a nitroreductase and a disproportionation agent.

This invention discloses a near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe, its preparation method, and its application, belonging to the field of medical detection technology. The structural formula of the near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe is as follows: The near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe of this invention possesses a hypoxia dual-mode visual monitoring molecular framework, enabling dual-mode determination of hypoxia microenvironments. It improves the stability of the cyanine near-infrared framework and achieves highly stable and specific detection of nitroreductase under hypoxia. Under hypoxia conditions, the product reacts with NTR, and the single-photon excited near-infrared fluorescence is quenched and then illuminated. Simultaneously, under hypoxia, the probe reacts with Cys to generate a two-photon excited fluorescence signal, effectively avoiding the probe consumption problem of dual-response systems and ensuring the non-interference of fluorescence excitation and emission (dual-mode), thus truly achieving accurate locking of hypoxia microenvironments.

The invention discloses a xanthene nitroreductase-responsive fluorescent dye compound as well as a preparation method and application thereof, and belongs to the technical field of xanthene derivatives. The fluorescent dye compound can be used for quickly and specifically recognizing overexpressed nitroreductase in tumor cells; after the fluorescent probe acts with nitroreductase, the fluorescence intensity of the fluorescent probe at 718 nm is obviously enhanced, and the fluorescent probe has excellent targeting capability on mitochondria; meanwhile, under the excitation of illumination with the specific wavelength of 660 nm, an acting product of the fluorescent dye compound and nitroreductase can efficiently generate reactive oxygen species (ROS), and then the effect of specifically killing tumor cells is achieved. The xanthene nitroreductase response type fluorescent dye compound provided by the invention can achieve the goal of integration of diagnosis and treatment of solid tumors, and has important clinical application value.

The present invention belongs to the field of medicinal chemistry technology and relates to a lysosomal-targeted nitroreductase-responsive fluorescent probe based on a triphenylamine backbone, its synthesis method, and its application. The triphenylamine derivative-based lysosomal-targeted nitroreductase-responsive fluorescent probe provided by the present invention does not emit light upon initial entry into cells. However, after a period of culture in cancer cells, the electron-withdrawing nitro group is reduced by the intracellular nitroreductase. Under laser irradiation, the charge within the fluorophore shifts, resulting in fluorescence. The present invention also provides a method for synthesizing the lysosomal-targeted nitroreductase-responsive fluorescent probe and its use in bioimaging.

The invention relates to synthesis and anti-tumor application of a nitroreductase activated camptothecin chemiluminescent prodrug. The prodrug molecule (CPT-NBz-CL) integrates three key functional units through a 2, 6-hydroxymethyl p-cresol derived self-burning group: (i) a nitrobenzene group (NBz) as a nitroreductase (NTR) specific trigger; (ii) a potent chemotherapeutic drug camptothecin (CPT) as a cytotoxic load; (iii) a Schaap type phenolic group-dioxetane structure is used as a chemiluminescence reporter group (CL), and the structure is shown as a formula (I). In order to carry out comparative research, a control molecule CL-NBz-CL which lacks a CPT load but retains a trigger and a reporter group is synchronously synthesized. The structures and optical properties of the two molecules are comprehensively characterized through analysis means such as nuclear magnetic resonance hydrogen spectrum, carbon spectrum, absorption spectrum, fluorescence spectrum and luminescent spectrum, and the molecules can verify that the molecules have specific detection capability on NTR through chemiluminescence, fluorescence spectrum, selectivity and the like in vitro.

This invention belongs to the field of fluorescent probe technology, specifically relating to a nitroreductase fluorescent probe, its preparation method, and its application. To develop an NTR fluorescent probe with high selectivity, high sensitivity, short response time, and long excitation and emission wavelengths, this invention utilizes pentamethine cyanine dye and a "ester group → carboxyl group" conversion strategy at the central site of this dye to develop a nitroreductase fluorescent probe, Cy5-NTR. In the presence of NADPH, the nitro group of the probe is first catalyzed by the overexpression of NTR by cancer cells, resulting in a single-electron transfer and the generation of a nitro anion radical. This radical is then further reduced to an amino group, followed by a 1,6-rearrangement and elimination reaction, releasing the strongly fluorescent centrally carboxyl-substituted pentamethine cyanine dye Cy5-COO. This probe can not only distinguish cancer cells / tissues from normal cells / tissues with high contrast under hypoxic conditions, but also provide in situ real-time imaging of tumors in tumor-bearing mice.

The application belongs to the field of agricultural biotechnology and environmental microbial engineering, and discloses a mesotrione nitroreductase Mtnr, a coding gene thereof and application. The nucleotide sequence is SEQ ID NO. 1, the full length is 756 bp, and 251 amino acids are encoded, and the amino acid sequence is SEQ ID NO. 2. The highest homology of Mtnr with a reported nitroreductase is only 50.6%, and therefore it is a new nitroreductase gene. The nitroreductase Mtnr provided by the application can catalyze the nitroreduction of mesotrione, and convert mesotrione into a product 2-amino-4-methylsulfonylbenzoic acid (AMBA) without herbicidal activity, indicating that the Mtnr gene has great application prospect in mesotrione biodegradation detoxification and remediation.

The invention belongs to the technical field of medicine preparation, and particularly relates to a ferroptosis agonist, an anoxia response type nanometer co-delivery system and application thereof. The novel ferroptosis agonist RSL3-ClAc is synthesized by introducing a chloracetyl fragment into an RSL3 molecule, the binding capacity of the novel ferroptosis agonist RSL3-ClAc with GPX4 is remarkably enhanced, and then the ferroptosis induction efficiency is improved. And meanwhile, the RSL3-ClAc and the hypoxia response type photosensitizer TCy5-NO2 are subjected to self-assembly, so that the nano drug delivery system is constructed. According to the nano drug delivery system, the EPR effect and the hypoxia response characteristic of the nano carrier are utilized, the tumor barrier can be effectively broken through, and the focus can be accurately positioned. In a tumor hypoxic microenvironment, the activation of a Try5-NO2 photosensitizer is triggered by nitroreductase (NTR), and RSL3-ClAc is synchronously released to enhance the ferroptosis induction effect.

This invention relates to the field of microbial technology, disclosing a *Strombus adhesiveus* strain YF2 that degrades polyethylene mulch film, microbial preparations, methods, and applications, addressing the problems of insufficient efficacy and unclear mechanisms in existing PE-degrading strains. This strain is deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC No:67086, and a deposit date of October 13, 2025. Through systematic parallel comparisons, strain YF2 significantly outperformed the control strain in PE degradation efficiency, biofilm formation ability, and induction of PE polymer oxidation. Genomic analysis showed that YF2 contains a genetic network encoding key degrading enzyme systems such as nitroreductase, cytochrome P450, and alkane monooxygenase, and particularly possesses unique nitrification degradation potential. Microbial preparations prepared based on this strain can be used for the bioremediation of PE mulch film residues in farmland soils, compost, and other environments.

The present application relates to a kind of based on heptamethine cyanine molecule, its preparation method and application, belong to medical technology field.Compounds B1-3, C1 and C3 can specifically examine nitroreductase, be activated by nitroreductase and photoacoustic signal is enhanced.Introducing heavy atom, significantly improve photo-thermal conversion efficiency and intersystem crossing, promote photothermal therapy and photodynamic therapy.Compound C1 shows specific tumor killing effect and obvious photoacoustic signal in vivo by excellent synergistic PTT / PDT effect, provides a general strategy for cancer photoacoustic diagnosis and enhanced phototherapy.Compound C3 realizes staphylococcus aureus in situ pneumonia photoacoustic imaging for the first time, and can detect bacteria in tumor with high sensitivity and specificity, provides a promising new method for early detection of deep bacterial infection in vivo.

The invention discloses a preparation method and application of a pH / nitroreductase double-response type fluorescent probe, and belongs to the technical field of fluorescence detection. The probe is specifically activated only when the probe and nitroreductase coexist in an acidic microenvironment (pH = 6-7), fluorescence Turn-On response is achieved, the emission wavelength of the probe is located at 760-850 nm (near-infrared window), and the fluorescence intensity is enhanced by 13-132 times after the probe is activated. The probe has the advantages of high specificity, near-infrared quick response (the fluorescence is the strongest within 0-30 minutes after double conditions are met at the same time) and high sensitivity. In addition, the unique double-response mechanism effectively avoids the interference of a single factor, and the detection specificity and the signal-to-noise ratio in a complex biological system are remarkably improved.

The invention belongs to the technical field of microorganisms, and provides a gene combination for degrading organic arsenic, an engineering bacterium and application of the gene combination. The gene combination comprises a nitroreductase gene BsntrA, an arsenate reductase gene ArsC2 and an efflux pump gene BsexpA which are from brevundimonas M20, the gene combination is used for constructing a recombinant expression vector and transforming the recombinant expression vector into a host cell, the obtained gene engineering cell forms an organic arsenic reduction-secretion system through protein expressed by the gene, nitro reduction, arsenic (V) reduction and HAPA (III) secretion of roxarsone are achieved, the degradation rate of the gene engineering cell to roxarsone in wastewater reaches 75.6%, and the gene engineering cell has a good application prospect. The drug resistance to roxarsone is obviously improved, and the compound can be efficiently used for removing organic arsenic in pollutants.

The invention provides a dual-response fluorescent probe. The fluorescent probe has dual response on tumor microenvironment viscosity and nitroreductase. The synthesis raw materials of the probe compound are easy to obtain, the synthesis operation is simple, and the preparation is convenient; and the fluorescent probe has strong specificity and high sensitivity for tumor recognition, and is safe and non-toxic.

The present invention relates to the technical field of fluorescent probes, and in particular to a fluorescent probe for rapid detection of nitroreductase, a preparation method thereof, and an application thereof. The present invention provides a fluorescent probe for rapid detection of nitroreductase, a preparation method thereof, and an application thereof. The fluorescent probe is named DM-CN. Due to the quenching effect of the nitro group, DM-CN is almost non-fluorescent. However, when DM-CN reacts with NTR in the presence of NADH, the 4-nitrobenzene substrate is reduced to the corresponding amine by NTR, the ether bond is broken, and then the phenol oxygen undergoes an intramolecular nucleophilic attack on the nitrile group to form iminocoumarin, and then the fluorescence emission is significantly enhanced, thereby achieving specific recognition of nitroreductase. The fluorescent probe DM-CN has the characteristics of high specificity, high sensitivity, high fluorescence intensity response, short response time, and little damage to organisms, and can be used for the detection of nitroreductase in A549 cells and zebrafish.

The invention relates to the technical field of fluorescent probes, and discloses a pH-NTR cascade response type nanoprobe, a preparation method and application, the nanoprobe is formed by encapsulating an NTR response type fluorescent probe CyBrN by a liposome self-assembled by a pH response type molecule DCPA, and the nanoprobe can be subjected to structural dissociation in a tumor acidic microenvironment and realize controllable release of probe molecules, so that the pH-NTR cascade response type nanoprobe can be used for detecting tumor tumors. The released probe can be specifically recognized by overexpressed nitroreductase in tumor cells and is subjected to reduction activation, so that a near-infrared fluorescence signal is recovered, and accurate positioning of the tumor cells is realized; meanwhile, the released probe efficiently generates active oxygen under laser irradiation, precise photodynamic killing of tumor cells is achieved, and the probe can be used as a PDT photosensitizer.

The invention provides a nitroreductase mutant and application thereof in preparation of p-anisidine, and relates to the technical field of biology. The nitroreductase mutant provided by the invention is obtained by mutating wild type nitroreductase with an amino acid sequence as shown in SEQ ID NO.1, and the mutation comprises one of the following mutations: arginine at the 224th site is mutated into glycine; asparagine at the 43rd site is mutated into threonine; the alanine at the 218th site is mutated into tryptophan; glutamic acid at the 166th site is mutated into lysine. Compared with wild type nitroreductase, the nitroreductase mutant is higher in enzyme activity, p-nitroanisole can be efficiently catalyzed to generate p-aminoanisole under the synergistic catalysis of V2O5, the nitroreductase mutant is further used for preparing alzapine, and meanwhile, under the system, the substrate spectrum of nitroreductase catalysis is expanded.

This invention provides a nitro-aryl ring-substituted imidazopyrazine compound that can be used as a bioluminescent probe for detecting nitroreductases. In the NanoLuc luciferase bioluminescent system, this probe exhibits good responsiveness in in vitro experiments and can be selectively reduced by NTRs. Viable bacterial experiments and imaging experiments in a mouse model of bacterial infection both demonstrate that this probe is a highly selective and sensitive luminescent probe for detecting NTR activity, enabling the detection and imaging of NTR activity in vivo.

The invention belongs to the technical field of environmental pollutant monitoring and biological imaging analysis, and provides a fluorescent probe for detecting superoxide anions and nitroreductase as well as preparation and application of the fluorescent probe. The chemical structural formula of the fluorescent probe is shown in the specification. The probe can realize rapid and accurate detection through the change of a fluorescence signal, and provides a new tool and method for physiological and pathological monitoring of animals under environment exposure.

The invention discloses a preparation method and application of a nitroreductase response hydrogen sulfide donor with a near-infrared fluorescence report function, and belongs to the technical field of medicine preparation. The preparation method comprises the following steps: mixing m-nitrophenol, K2CO3 and IR-780 iodide in a first organic solvent, reacting, extracting, and carrying out rotary evaporation to obtain a finished product 1; dissolving the finished product 1 in a second organic solvent, co-heating and refluxing with tin chloride and concentrated hydrochloric acid, adding an alkaline solution, and carrying out suction filtration, extraction, rotary evaporation, sand making and column chromatography to obtain a finished product 2; slowly dripping thiophosgene into the third organic solvent solution of the finished product 2, fully reacting, extracting, performing rotary evaporation, making sand, and passing through a column to obtain a finished product 3; and dissolving 4-nitrobenzyl alcohol, the finished product 3 and NaH in a fourth organic solvent, reacting under an ice bath condition, and carrying out sand making, column passing and rotary evaporation to obtain the hydrogen sulfide donor. The hydrogen sulfide donor provided by the invention is helpful for promoting the clinical application of hydrogen sulfide therapy in the aspects of clinical application and research value.

The invention relates to the technical field of chemiluminiscence detection, in particular to a rapid detection reagent in a chemiluminiscence mode and a preparation method of the rapid detection reagent, and the rapid detection reagent is characterized in that a core component of the rapid detection reagent is a nitroreductase-responsive flash type end product 5 as shown in a formula I, and the concentration is 0.1 mu M; the auxiliary reagent comprises reduced nicotinamide adenosine dinucleotide (NADH) and a Tris-HCl buffer solution (pH is 7.4), and the concentration of the reduced nicotinamide adenosine dinucleotide is 5 mu M. The reagent is used for detecting nitroreductase (NTR), a high-intensity chemiluminescence signal can be generated within 1 minute after the reagent acts with the NTR at 37 DEG C, the signal duration is larger than or equal to 40 minutes, and the NTR detection limit reaches 0.951 ng / mL. The problems that an existing flash type probe is complex in synthesis process, short in signal duration and insufficient in low-concentration NTR detection capacity are solved.

The present invention relates to a dual fluorescence probe for detecting nitroreductase and hydrogen sulfide, and a manufacturing method therefor. The dual fluorescence probe according to the present invention can detect both nitroreductase and hydrogen sulfide, which are indicators of a hypoxic environment, and can provide low background fluorescence noise and selective, high fluorescence signals, thus enabling normal cells and tumor cells in a hypoxic microenvironment to be accurately distinguished and detected through improved hypoxia detection effects and imaging capabilities.

The application belongs to the technical field of medicine, and discloses a fluorescent probe for detecting nitroreductase (NTR) activity, a preparation method and use thereof. The probe is constructed by coupling p-nitrobenzyl bromide and a hemicyanine fluorescent group, can specifically release the fluorescent group under the action of NTR, and produce a significant fluorescent signal. The probe is sensitive in response in a biological system, has excellent biocompatibility, and shows good application potential in the fields of tumor diagnosis and biological imaging.

This invention provides a two-photon fluorescent probe for detecting hypoxia levels. The probe comprises a core fluorophore composed of a 2-dimethylamino-7-hydroxynaphthalene two-photon fluorophore and a probe composed of a sulfonate methylene group. This invention also provides a method for preparing and applying the two-photon fluorescent probe for detecting hypoxia levels. The fluorescent probe of this invention enables the monitoring of hypoxia levels in tumor and normal tissues by in-situ detection of nitroreductase (NTR) content within tissues. It features fast response, high sensitivity, high optical stability, good biocompatibility, high selectivity, and high depth, high resolution, and low biological background in two-photon imaging. In cell imaging applications, this probe exhibits good cell permeability and can detect NTR content at the cellular level, indicating cellular hypoxia.

The invention provides a naphthalimide type fluorescent probe and a preparation method and application thereof.The naphthalimide type fluorescent probe is of a structure shown in the formula I. The naphthalimide type fluorescent probe takes 4-nitrobenzyl bromide as a recognition group and a fluorescence quencher, and 1, 2, 3, 4-tetramethyl-1, 3, 4-tetramethyl-1, 3, 4-tetramethyl-1, 3, 4-tetramethyl-1, 3-tetramethyl-1, 3-tetramethyl-1, 3-tetramethyl-1, 3-tetramethyl-1, 3-tetramethyl-1, 3-tetramethyl-1, 3-tetramethyl-1 and naphthalimide modified by 2, 3, 3-tetramethyl-3H-indole iodide is used as a fluorophore. When nitroreductase responds to the probe, a fluorescence signal is enhanced, and the probe has the advantages of low detection limit, good stability, rapid response and the like, and can be used as a tool for quantitative research of nitroreductase.

The present invention discloses a NIRF / PA dual-modal probe based on NTR response, a preparation method thereof, and an application thereof, belonging to the field of biomedicine technology. The NIRF / PA dual-modal probe based on NTR response can rapidly respond to NTR, with response saturation in about 5 minutes, good selectivity for nitroreductase, and high sensitivity. The probe absorbs and emits in the near-infrared region, which can effectively overcome interference from biological background signals. The probe is a fluorescence / photoacoustic dual-modal ratiometric probe, which can achieve self-correction during the imaging process of biological systems and improve imaging accuracy. The probe realizes near-infrared fluorescence / photoacoustic dual-modal ratiometric imaging of pancreatic cancer tumors in mice, reflecting the clinical application prospects of the present invention in imaging-mediated pancreatic cancer tumor surgery.

The invention belongs to the technical field of genetic engineering and environmental remediation, and particularly discloses construction of nitroreductase delivery plasmids and application of the nitroreductase delivery plasmids in environmental remediation. The nucleotide and amino acid sequences of the nitroreductase gene derived from the actinomycetes S16 functional metagenome are shown as SEQ ID NO. 1 and SEQ ID NO. 2. The method comprises the following steps: designing a primer by taking pQ-SacB-araC plasmid as a template, and carrying out PCR (Polymerase Chain Reaction); the method comprises the following steps: cloning an actinomycete S16 functional metagenome as a template, and designing a primer according to a target nitroreductase gene sequence to carry out PCR (Polymerase Chain Reaction) amplification; and purifying and recovering the two amplification products, and carrying out fragment recombination to obtain the nitroreductase delivery plasmid pQ-NR. The delivery plasmid pQ-NR and recombinant bacteria capable of stably and efficiently expressing nitroreductase are constructed, efficient delivery of nitroreductase genes in various microorganisms is achieved, and the application effect of the nitroreductase genes in actual environmental remediation related to nitro compound pollution is enhanced.

Provided is a compound comprising the structure:(SIG)-(SI-MOD)m.In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.

The invention relates to the technical field of fluorescent probes, in particular to a near-infrared fluorescent probe TM-N1 with good water solubility for detecting nitroreductase as well as a preparation method and application of the near-infrared fluorescent probe TM-N1. The good-water-solubility fluorescent probe TM-N1 for near-infrared red light detection of nitroreductase, provided by the invention, has the advantages that the water solubility is relatively good, the preparation method is simple, and the wavelength of the probe can reach a near-infrared band; the probe has high sensitivity and good selectivity. The method can be used for preparing reagents for detecting hypoxia lesion sites of organisms, can also be used for preparing tumor cell detection reagents, and has wide application prospects in the field of preparation of biological living body imaging analysis reagents and tumor cell detection reagents.

The present invention relates to the field of fluorescent probe technology, and in particular to a near-infrared fluorescent probe TM-N2 with good water solubility for detecting nitroreductase, as well as its preparation method and application. The present invention provides a fluorescent probe TM-N2 with good water solubility and near-infrared red light for detecting nitroreductase. The probe has good water solubility, a wavelength that can reach the near-infrared band, and a simple preparation method. The probe has high sensitivity and good selectivity. The probe has achieved detection of nitroreductase under hypoxic conditions in zebrafish and in tumor cells, and has broad application prospects in the fields of in vivo imaging analysis and tumor detection.