119 results about "Nitroreductase" patented technology

A method utilising one or more fluorogenic probes, for the detection of nitroreductase activity. The non-fluorescent probes are reduced in the presence of nitroreductase to form fluorescent derivatives that may be detected using fluorescence spectroscopy. In particular, the method may be used to detect and/or identify a plurality of nitroreductase in a single test environment

The invention discloses a novel dimolecular fluorescence probe of which the chemical name is 6-((4-nitrobenzyl)oxy)-2,3,4,4a-tetrahydro-xanthene-1-one. The invention also discloses application of the two-photon fluorescence probe in detecting nitroreductase in cells. The fluorescence intensity of the probe obviously increases as the nitroreductase content increases. The probe disclosed by the invention can be used for detecting the nitroreductase content in the tumor anoxic zone by a fluorescent imaging technique, thereby evaluating and researching the anoxic level of the tumor anoxic zone.

A process for biological production of ortho-aminophenols from nitroaromatic compounds using recombinant E. Coli strains. The process uses an enzyme system that makes use of a nitroreductase enzyme that initially reduces the nitroarene to the hydroxylaminoarene and a mutase enzyme that converts the hydroxylaminoarene to an ortho-aminophenol.

The invention discloses a nitroreductase responsive hypoxic probe compound, preparation and application thereof, and relates to a preparation method of a fluorescent probe compound (I) capable of responding to nitroreductase, and application of the fluorescent probe compound (I) in hypoxia analysis and imaging. The fluorescent probe disclosed by the invention can realize high-sensitivity and high-specificity response to tumor microenvironment hypoxia related nitroreductase, is prepared through a condensation reaction, and has the significantly increased conjugation degree of molecules, so thatthe absorption and fluorescence emission wavelengths have obvious red shift compared with coumarin, rhodamine and other dyes, and when the hypoxic probe is used for analysis and imaging application,the interference is small; and the probe is constructed through an intramolecular charge transfer mechanism (ICT), is a signal enhanced probe and has a small background signal.

This invention describes the coding gene and applications of a nitrobenzene nitro-reducing enzyme, comprising a protein from the following amino acid residue sequences: 1) SEQ ID No. 1 in the sequence table; 2) the amino acid residue sequence in SEQ ID No. 1 is a protein substituted, deleted or added with 1-10 amino acid residues and having the nitrobenzene nitro-reducing enzyme function. The nitrobenzene nitro-reducing enzyme and its coding gene in this invention play an important role in the production of ortho-aminophenols and their derivatives.

Disclosed are nitro-substituted squaraine reporter dyes and methods using such dyes for detecting nitroreductase enzyme activity and nitroreductase gene expression in cellular assays. The dyes are of the structure:

in which Z¹ and Z² independently represent a phenyl or a naphthyl ring system; X and Y are selected from oxygen, sulphur, —CH═CH— and the group:

R¹ and R² are selected from C₁-C₄ alkyl, —(CH₂)_n—P, —{(CH₂)₂—O}_p—R⁶ and group W; where P is selected from COOR⁷, SO₃⁻ and OH, W is mono- or di-substituted nitrobenzyl, R⁶ is methyl or ethyl, R⁷ is selected from H, C₁-C₄ alkyl and CH₂OC(O)R⁸, where R⁸ is methyl, or t-butyl, n is an integer from 1 to 10, and p is an integer from 1 to 3; R³ and R⁴ are selected from hydrogen, NO₂, halogen, SO₃⁻, C₁-C₄ alkoxy and —(CH₂)_m, —COOR⁷; where R⁷ is hereinbefore defined and m is 0 or an integer from 1 to 5; R⁵ is C₁-C₆ alkyl optionally substituted with COOR⁷, SO₃⁻, or OH; where R⁷ is hereinbefore defined; and at least one of groups R¹, R², R³ and R⁴ comprises at least one N0₂ group. Also provided are methods for screening for a test agent whose effect upon nitroreductase enzyme activity and nitroreductase gene expression is to be determined.

The invention discloses a bacteria nitroreductase detection kit and a special-purpose fluorescence probe thereof. The structural formula of the fluorescence probe is shown in the formula I. The bacteria nitroreductase detection kit comprises a reagent stock solution 1 and a reagent stock solution 2, wherein the reagent stock solution 1 is a buffer solution containing coenzyme; the reagent stock solution 2 is a solution of the fluorescence probe. Experiments prove that the fluorescence intensity of the probe is very weak, and the probe has 1,6-rearrangment elimination reaction after nitroreductase is added, so that resorufin fluorescence parent is released, the solution fluorescence is remarkably strengthened and the color is changed into purple red from colorless, and therefore, the method can be used for detecting nitroreductase. The bacteria nitroreductase detection kit is simple to operate, low in cost, quick, efficient, sensitive and the like, is easy to popularize and apply, and has great application prospect in the field of medicine and the like. The structural formula is shown in the description.

Provided is a compound comprising the structure:

(SIG)-(SI-MOD)_m.

In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.

The invention discloses a class of near infrared fluorescent dyes, a targeted imaging agent thereof, a nanocarrier, an anticancer drug and an application. The near-infrared fluorescent dyes and hydrophilic dendrimers are linked by environmentally sensitive bonds to form amphiphilic dendrimers with self-assembly capability. The hydrophilic dendrimers have good tumor targeting performance and transmembrane performance when lysine and arginine are used as backbone structures. The amphiphilic dendrimers also have nitroreductase responsiveness and can be self-assembled into nanoliposomes or micelles or vesicles for gene and/or drug carriers that are disassembled in the tumor microenvironment to release genes and/or drugs for therapeutic purposes. The amphiphilic dendrimers can also produce reactive oxygen in near infrared light and can be used in photodynamic therapy. The dendrimers are fluorescence quenched before and after self-assembly and after disassembly, and can generate fluorescenceenhancement only under the action of nitroreductase of tumor cells. The penetrating capability is higher, and the penetrating is more precise.

The invention provides a fluorescence probe for detecting nitroreductase and application of the fluorescence probe. The structural formula of the fluorescence probe is shown in the description. The fluorescence probe detects the activity of the nitroreductase through the fluorescence difference of a reactant and a product.

The invention relates generally to bacterial nitroreductase enzymes and methods of use thereof: More particularly, although not exclusively, said enzymes have use in non-invasive imaging techniques, monitoring of therapeutic cell populations and gene-directed enzyme prodrug therapy. The invention also relates to the use of bacterial nitroreductase enzymes in radioimaging and/or ablation of biological agents and to compositions of use in such methods.

The invention discloses a long-life fluorescein derivative with a hypoxia targeting function as shown in a structural formula I, a synthesis method of the derivative and a biological application of the derivative. In the formula, L is -CH2-O-CH2-, CO-O-CO- or -COO-CH2-, and R is a symmetric C10H8N2O, C8H8O or C6H6OS structure. The long-life fluorescein derivative I can be used for detecting nitroreductase (NTR) in hypoxic tumor tissues. With long triplet-state life, the derivative I can produce a singlet state for photodynamic therapy of hypoxic tumors. The fluorescein derivative based on traditional stains has integrated diagnosis and treatment effects and has the potential of playing a role in biological application.

The invention belongs to the technical field of chemical analysis, and relates to a small molecular fluorescent probe, particularly to a nitroreductase fluorescent probe based on nitro reduction and sulfur-nitrogen transposition, wherein the fluorescent probe is a BODIPY structure nitroreductase fluorescent probe. The invention discloses preparation and applications of the probe and a novel reaction mechanism for nitroreductase recognition. The fluorescent probe disclosed by the invention is stable in photophysical activity and high in nitroreductase response sensitivity, and can be applied tofluorescence imaging detection of hypoxic tumor cells.

An herpes simplex virus wherein the herpes simplex virus genome comprises nucleic acid encoding a nitroreductase (NTR) is disclosed. Disclosed herpes simplex viruses are indicated to be useful in the treatment of cancer which may involve gene directed enzyme prodrug therapy.

The invention relates generally to compounds and methods for imaging and/or selective ablation of nitroreductase-expressing cells and/or biological agents. More particularly, although not exclusively, the invention provides compounds that are selectively metabolised by bacterial nitroreductases and are substantially insensitive to metabolism under oxic or hypoxic conditions by human nitroreductase enzymes.