Nitroreductase gene lnr as well as coded proteins and application thereof

Abstract

The invention discloses nitroreductase genes lnr as well as coded proteins and application thereof. The nitroreductase genes lnr has a nucleotide sequence of SEQ ID NO.1, has the full length of 609bp,is capable of coding 202 amino acids, and has an amino acid sequence of SEQ ID NO.2. The LNR is capable of performing catalytic reduction on the nitro group at the site C-6 of a pendimethalin benzenering to produce 2-nitro-6-amino-N-(1-ethylpropyl)-3,4-dimethyl aniline, and further performing catalytic reduction on two symmetrical nitro groups on the benzene ring of butralin, oryzalin and trifluralin, but the catalytic efficiency is obviously higher than that of PNR. The LNR has a broad substrate spectrum, and has wide application prospects in the aspect of eliminating environmental contamination and remediation caused by nitro-aromatic herbicide residues.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms and agriculture, and relates to a nitroreductase gene lnr for degrading pendimethalin, secbutaline, amisulfurin and trifluralin, its encoded protein and its application. Background technique [0002] With the improvement of the requirements for grain production, the area of ​​chemical weeding is also gradually expanding worldwide, and the application amount of herbicides also remains high. The use of herbicides can reduce agricultural labor intensity, ensure normal agricultural production and even high yield, but the long-term use of a large number of herbicides has inevitably produced negative effects: pesticide pollution in soil, groundwater and air; residues in certain crops ; Potential hazard to human health. A large number of studies have shown that microbial degradation is the most important factor for the disappearance of pesticides in the environment, and microbial degradation ...

AI Technical Summary

Problems solved by technology

Studies have shown that the main ways of microbial degradation of dinitroaniline herbicides are oxidative N-terminal dealkylation and nitro reduction, but the whole process of microbial degradation of dinitroaniline herbicides is still unclear, and the degradation Genes / enzymes are rarely reported

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