7 results about "Fluorescent derivative" patented technology

The invention provides a method for qualitatively or quantitatively detecting urea in grains or distiller's yeast, and belongs to the technical field of food detection. The pretreatment method for detecting urea in grain or distiller's yeast provided by the invention comprises urea extraction and derivation steps: the urea extraction step comprises the following steps: mixing a grain or distiller's yeast sample with activated carbon, a zinc acetate solution and a potassium ferrocyanide solution, carrying out ultrasonic treatment, filtering with medium-speed filter paper, discarding 1-2mL of primary filtrate, and collecting subsequent filtrate as a sample solution; the derivation method comprises the following steps: mixing a sample solution, a 9-hydroxyton solution and a hydrochloric acid solution, placing in a dark place, and filtering to obtain a to-be-detected solution; according to the pretreatment method provided by the invention, interference substances in the sample are removed through the steps of activated carbon adsorption, zinc acetate and potassium ferrocyanide precipitation, the matrix effect is reduced, further, 9-hydroxyton is adopted as a derivatization reagent and reacts with urea to generate a fluorescent derivative, the extraction efficiency is high, and the detection sensitivity and accuracy are improved.

The invention discloses a method for rapidly detecting acrylamide in food by using a fluorescent probe, which comprises the following steps of: synthesizing copper and iron-containing phosphomolybdic acid nano-enzyme (Cu, Fe-PMos) with peroxidase-like nano-enzyme activity by using phosphomolybdic acid, copper chloride and ferric chloride as precursors through a microwave method, catalyzing xanthol to react with HCl to generate a fluorescent derivative by using the Cu and Fe-PMos, and detecting the acrylamide in the food by using the fluorescent probe. When acrylamide is added into a fluorescent system, reaction of xanthene alcohol and acrylamide is carried out to generate a xanthene acrylamide fluorescent substance, reaction of Cu, Fe-PMos and xanthene alcohol is inhibited, and the fluorescence intensity of the system is reduced, so that a fluorescent probe rapid detection method of acrylamide is established. Other coexisting substances do not interfere with each other, the detection limit reaches 0.01 mu g / mL, and the method has the characteristics of high sensitivity, strong specificity, simplicity and rapidness in operation and the like.

This application relates to the technical field of vaccine quality testing, specifically to a quantitative detection method for 3D-MLA in liposome adjuvants. The method includes the following steps: taking a test sample solution, adding hydrochloric acid for acid hydrolysis to completely hydrolyze 3D-MLA into D-glucosamine and D-glucosamine-6-phosphate; adding an internal standard solution and a derivatizing reagent to the hydrolyzed sample to perform a derivatization reaction, converting the glycosamine and internal standard into fluorescent derivatives; detecting the derivatized sample using high-performance liquid chromatography-fluorescence detector; and calculating the 3D-MLA content in the test sample using a standard curve method based on the ratio of the total peak area of ​​glucosamine to the peak area of ​​the internal standard. The detection method provided in this application is highly specific, sensitive, easy to operate, and suitable for large batches of samples.

This invention discloses the application of a thiazo[a]thiazolium fluorescent derivative in the sensing and detection of butyl nitrite. This fluorescent derivative uses the electron-deficient thiazo[a]thiazolium group as the core, with symmetrically connected at both ends a benzene ring with a typical "propeller" conformation and methoxylated triphenylamine at the para- or ortho-position as electron donors, forming a D-A-D type structure. This derivative exhibits high fluorescence quantum yield, excellent intramolecular charge transfer (ICT) characteristics, and good photophysical properties, demonstrating excellent sensing performance for butyl nitrite, including high sensitivity, fast response speed, and short recovery time. Furthermore, this fluorescent derivative is prepared into a fluorescent sensing film with good photochemical stability using a drop-coating method, and then assembled into a device to construct a thin-film fluorescent sensor, demonstrating significant application potential and practical value in the high-sensitivity detection of butyl nitrite.

The invention belongs to the technical field of analytical chemistry, and discloses an analysis method for determining the dissolution amount of HMDI in a medical instrument containing a polyurethane material, and the method comprises the following specific steps: 1, preparing a solution, namely preparing a mobile phase, a diluent, a derivatization reagent, a derivative solvent, a standard stock solution, a series of standard curve solutions, a process blank solution and an accuracy solution; the specific analysis method for the dissolution amount of the HMDI in the medical instrument containing the polyurethane material is established, the blank in the prior art is filled, the problem that the HMDI residue detection in the medical instrument industry lacks a unified standard is solved, and a reliable technical means is provided for product safety evaluation; meanwhile, 9-(methylaminomethyl) anthracene is adopted as a derivatization reagent and can be subjected to a specific reaction with the HMDI to generate a stable fluorescent derivative, and a fluorescent detector is combined for detection, so that the sensitivity of the method is remarkably improved, and the detection requirement of the dissolution amount of the low-concentration HMDI can be met.

The invention relates to a PDRN content detection method, and belongs to the technical field of PDRN detection. The detection method comprises the following steps: (1) diluting a PDRN sample to be detected, and adding a derivatization reagent to obtain a test solution; (2) preparing a reference solution; (3) respectively detecting the test solution and the reference solution by adopting an ultra-high performance liquid chromatography-fluorescence detection method, and drawing a standard curve; and calculating the content of the PDRN sample to be detected according to the peak area of the test solution, the standard curve and the dilution ratio. The derivatization reagent composed of dansyl chloride and sodium bicarbonate reacts with PDRN to generate a derivative with a strong fluorescence characteristic, so that the detection specificity can be effectively enhanced, the impurity interference can be reduced, and the detection accuracy and sensitivity can be improved. And moreover, the detection time is greatly shortened, each detection process can be completed within 22 minutes, and the efficiency is remarkably improved compared with that of a traditional method. Meanwhile, the PDRN as low as ng / mL level can be detected by the method disclosed by the invention, and the trace detection requirement is met.

The scheme discloses a series of corrole derivatives in the field of pharmaceutical chemistry and a preparation method and application thereof, and the structural general formula of the corrole derivatives is shown as formula (I) or formula (II) or formula (III) in the text. The fluorescent compound of the application adopts a typical corrole intermediate, takes malonitrile or ethyl cyanoacetate as an electron acceptor, and introduces an active electron-donating group to form a conjugated system of push-pull electrons. The structural system after the cyclization of ethyl cyanoacetate creates more additional regions, and provides feasibility for the construction of new structures. Most of the compounds show good binding affinity to Aβ 42 aggregates, and the low cytotoxicity is verified, and the aggregates can be successfully used for the imaging of Aβ in vivo and in vitro.