38 results about "Glycosamine" patented technology

Compositions with synergistic anti-inflammatory effects in inflammatory diseases resulting from activation and consequent degranulation of mast cells and followed by secretion of inflammatory biochemicals from the activated mast cells, the compositions containg one or more of a flavone or flavonoid glycoside a heavily sulfated, non-bovine proteoglycan, an unrefined olive kernel extract that increases absorption of these compositions in various routes of administration, a hexosamine sulfate such as D-glucosamine sulfate, S-adenosylmethionine, a histamine-1 receptor antagonist, a histamine-3 receptor agonist, an antagonist of the actions of CRH, a long-chain unsaturated fatty acid, a phospholipid, Krill oil, a polyamine, glutiramer acetate and interferon. Certain of the present compositions are useful in protecting against the neuropathological components of multiple sclerosis and similar inflammatory neurological diseases.

A medicinal composition in the form of tablet, capsule, dispersing tablet, effervescent tablet, etc for treating the various osteoarthritides is prepared from aminoglucose hydrochloride, chondroitin sulfate and pharmacologically acceptable additives.

The invention relates to a preparation method of a glycosylated surfactant. The preparation method comprises the following steps: firstly weighing glucose, dissolving the glucose in a methanol solution, then adding long-chain n-alkylamine according to the molar ratio of long-chain n-alkylamine to glucose of (1:1)-(1:6) to react with glucose to prepare alkyl glucosimine, then adding an excessive amount of NaBH4 to perform reaction to prepare an alkyl glycosamine crude product; sequentially washing the crude product with iced anhydrous ethanol and methanol solutions, and performing suction filtration to obtain alkyl glycosamine; further dissolving prepared alkyl glycosamine in the methanol solution, then adding glyoxylic acid according to the molar ratio of glyoxylic acid to alkyl glycosamine of (2:1)-(6:1) to react with alkyl glycosamine so as to prepare a glycosylated surfactant crude product; removing a reaction solvent and unreacted glyoxylic acid in the crude product by a rotating evaporation instrument to obtain the glycosylated surfactant. According to the preparation method provided by the invention, the novel glycosylated surfactant is prepared by reaction of glyoxylic acid and self-made alkyl glycosamine.

The present invention relates to health food with the function of preventing and auxiliarily treating osteoarthropathy, and is especially preparation process and formula of compound chondroitin sulfate tablet. The compound chondroitin sulfate tablet formula contains chodroitin sulfate 30-50 wt%, glycosamine chlorate 40-60 wt%, soybean isoflvone 2-3 wt%except supplementary material. The compound chondroitin sulfate tablet has both the treating function of chondroitin sulfate and the preventing function of soybean isoflvone, and is one kind of good health food with fast disintegrating, high leaching rate, easy taking and high absorption and other features.

This invention relates to a process for the preparation of glucosamine hydroiodide, glucosamine pyruvate, glucosamine phosphate or their mixtures with glucosamine hydrochloride, by electrodialysis. Starting from glucosamine hydrochloride, the exchange of the Cl− for an anion selected from the group consisting of SO4 −2, I−, CH3COCOO− and PO4−3 takes place during the process. The electrodialysis, that comprises a cathode, anode, and means of separation composed of anion exchange membranes, cation exchange membranes, bipolar membranes or by other suitable means of separation. This invention also relates to a glycosamine sulphate containing chlorides obtainable by the process, and its therapeutic use.

The invention relates to a synthesis method of a highly ordered dendronized heterogeneous glycopolymer containing various glycosyl groups. The method comprises the following steps: (1) adding anhydrous triethylamine into a dichloromethane solution of pentafluorophenol under an ice bath condition, then adding acryloyl chloride for reaction, and after the reaction is finished, washing, drying and purifying to obtain a compound PFPA; (2) carrying out polymerization reaction on the compound PFPA, a chain transfer agent and an initiator to obtain a polymer pPFPA; (3) mixing the polymer pPFPA with dendronized glycosamine, and carrying out ammonolysis reaction to obtain a glycopolymer; and (4) adding the glycopolymer into a sodium methoxide / methanol solution to react, and purifying after the reaction is finished, thereby obtaining the target product, namely the dendronized heterogeneous glycopolymer containing various glycosyl groups. Compared with the prior art, the method has the advantages that the advantages of RAFT polymerization and post-modification are combined, the glycopolymer with the ordered structure is obtained, particularly the regular heterogeneous glycopolymer is obtained, and a universal platform and the like are provided for preparation of the functional glycopolymer.

The invention provides a hepatic failure detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A, which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10 mM; a reagent B, which is prepared by mixing 0.01-10 U / 10 [mu] L of glycosamine acylase and 0.01-10 U / 10 [mu] L of sialidase, wherein the pH value of the mixed solution is 4-9; a reagent C, which is prepared by dissolving 8-aminopyrene-1, 3, 6-trisulfonic acid in DMSO (dimethylsulfoxide), and the concentration of the reagent C is 0.01 mM to 1M; and a reagent D: a stop solution. According to the invention, a glycome map in serum is determined by a detection reagent, and a peak value is quantified for statistical analysis, so that the establishment method of the hepatic failure serum glycome map model is provided for detecting hepatic failure.

The invention relates to a mixture of natamycin and mycosamine and to the use of these mixtures for sanitizing and subsequently preserving foodstuffs and beverages, and to a process for incorporatingthe claimed mixtures into foodstuffs and beverages.

The invention provides an esophageal cancer detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10 mM; a reagent B which is a mixed solution prepared by mixing 0.01-10 U / 10 [mu]L of glycosamine acylase and 0.01-10 U / 10 [mu]L of sialidase and has a pH value of 4-9; a reagent C which is prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO (dimethylsulfoxide) and has a concentration of 0.01 mM to 1M; and a reagent D which is a stop solution. According to the invention, a glycome map in serum is determined by a detection reagent, a peak value is quantified for statistical analysis, and the establishment method of the esophageal cancer serum glycome map model is provided for detecting esophageal cancer.

The invention provides an intestinal cancer detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10 mM; a reagent B which is a mixed solution prepared by mixing 0.01-10 U / 10 [mu]L of glycosamine acylase and 0.01-10 U / 10 [mu]L of sialidase and has a pH value of 4-9; a reagent C which is prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO (dimethylsulfoxide) and has a concentration of 0.01 mM to 1M; and a reagent D which is a stop solution. A glycome atlas in serum is measured through a detection reagent, a peak value is quantified for statistical analysis, and the establishment method of the intestinal cancer serum glycome atlas model is provided for detecting intestinal cancer.

The invention discloses an amphoteric glycolipid biosurfactant and a preparation method thereof, and belongs to the technical field of biological materials. The amphoteric glycolipid biosurfactant comprises fatty acid, polysaccharide, amphoteric glycolipid biosurfactant molecules, glycosamine, lipopeptide, ethanol, fatty amine and the like. The preparation method of the amphoteric glycolipid biosurfactant comprises the following steps: (1) culturing: carrying out staged culture by adopting three bacteria; (2) separating: adjusting the pH value of the obtained fermentation culture solution, centrifuging, and recovering a middle-layer clear solution; (3) performing acid precipitation: adjusting the pH value of the middle-layer clear solution, and then performing refrigerating precipitation;(4) filtering: collecting the precipitate by adopting a ceramic membrane, then dissolving the precipitate, and filtering again to obtain an aqueous solution; (5) concentrating: concentrating the aqueous solution in vacuum to obtain a concentrated aqueous solution; and (6) obtaining the finished product: adding the above components into the concentrated aqueous solution, and stirring. The preparedamphoteric glycolipid biosurfactant is a high-quality amphoteric glycolipid biosurfactant.

The glycosamine milk product is ewe / cow milk, semi-defatted ewe / cow milk, defatted ewe / cow milk, whole ewe / cow milk powder, defatted ewe / cow milk powder or yoghourt connecting glycosamine sulfate or glycosamine hydrochloride in 0.1-5 wt% and one or several of vitamin A, vitamin D, vitamin E and sodium glycocholate in 0.01-3 wt%. It is prepared through adding glycosamine sulfate or glycosamine hydrochloride and vitamin A, vitamin D, vitamin E and sodium glycocholate into the milk product through stirring, and high temperature sterilization. The present invention has the synergistic effect of the nutritious components in milk, glycosamine sulfate or glycosamine hydrochloride and vitamin A, vitamin D, vitamin E and sodium glycocholate, and thus calcium replenishing and other health functions, especially for the old people.

The invention discloses a plant infusion nutrient solution and a preparation method thereof. The solution comprises inorganic nutrient components, organic nutrient components and plant growth regulator components. The plant infusion nutrient solution can promote the growth of plants and increase the biomass of the plants, amino-oligosaccharin and 1-[4-(N-2-methoxybenzamide sulfonyl)phenyl]-3-methyl-urea are applied in a combined mode, and glycosamine and dicamba dimethylamine salt are applied in a combined mode, so that the plant infusion nutrient solution has a remarkable synergistic interaction function.

The invention provides a fatty liver detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A, which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10mM; a reagent B, which is prepared by mixing 0.01-10U / 10 microL of glycosamine acylase and 0.01-10U / 10microL of sialidase, and the pH value of the mixed solution is 4-9; a reagent C, which is prepared by dissolving 8-aminopyrene-1, 3, 6-trisulfonic acid in DMSO (dimethylsulfoxide), and the concentration of the reagent C is 0.01mM to 1M; and a reagent D: a stop solution. According to the invention, a glycome map in serum is determined by a detection reagent, and a peak value is quantified for statistical analysis, so that a method for establishing a model of a fatty liver serum glycome map is provided for detecting fatty liver.

The invention provides a prostate cancer detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10 mM; a reagent B which is a mixed solution prepared by mixing 0.01-10 U / 10 [mu]L of glycosamine acylase and 0.01-10 U / 10 [mu]L of sialidase and has a pH value of 4-9; a reagent C which is prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO (dimethylsulfoxide) and has a concentration of 0.01 mM to 1M; and a reagent D which is a stop solution. The invention provides a method for establishing a model of a serum glycome map of prostate cancer to detect the prostate cancer by measuring the glycome map in the serum through a detection reagent and quantifying peak values for statistical analysis.

The invention provides a hepatitis C liver cancer detection reagent and a preparation method thereof. The detection reagent is prepared by mixing the following reagents: a reagent A, which is prepared by adding 0.5-5% by mass of SDS into a 10 mM ammonium bicarbonate solution; a reagent B which is a mixed solution prepared by mixing 0.01-10 U / 10 [mu]L of glycosamine acylase and 0.01-10 U / 10 [mu]L of sialidase, and has the pH value of 4-9; a reagent C which is prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO (dimethylsulfoxide), and has the concentration of 0.01 mM to 1M; and a reagent D which is a stop solution. According to the invention, a glycome map in serum is determined by a detection reagent, and a peak value is quantified for statistical analysis, so that the invention provides a method for establishing a model of a hepatitis C liver cancer serum glycome map for detecting hepatitis C liver cancer.

The invention relates to the technical field of Amadori derivatives, provides a glycosamine Amadori derivative, and specifically defines the structural formula of the glycosamine Amadori derivative, and the structural formula of the glycosamine Amadori derivative comprises the glycosamine Amadori derivative with three structures obtained by reaction of three different amino-acid esters and fructose. The glycosamine Amadori derivative with the three structures can be subjected to pyrolysis under the heating condition, multiple products are obtained through pyrolysis, and the pyrolysis products are stable in chemical property, lasting in effect and high in aroma quality and can be used for improving the quality of cigarettes. Experimental results show that the glycosamine Amadori derivative provided by the invention can be pyrolyzed into various products with fragrance, and can bring more harmonious mouth feel during cigarette panel test, so that the smoking quality is improved.

The invention provides a drug-polypeptide self-assembled nanoparticles and a preparation method thereof. The particles are formed by self-assembling prodrug molecules under a hydrophobic effect after the prodrug molecules are synthesized by hydrophobic small-molecule drugs, phenylalanine dipeptide and hydrophilic glycosamine groups. The prodrug molecules are obtained after successful structural modification is carried out on the hydrophobic small-molecule original drugs, and the original drugs are wrapped by a compact spherical structure formed by self-assembly of the prodrug molecules, so that the original drugs have low toxicity and high water solubility. Compared with other drug-loaded nano-carriers, the nanoparticles solve the problems of low drug loading capacity and low encapsulation efficiency, have obvious advantages in drug loading capacity, and can be used for preparing anti-inflammatory or anti-tumor drugs.

A composition comprising Polyhydroxyurethanes, Glycosaminoglycans, hydrolysed glycosaminoglycans, glycosamines of glycosaminoglycans, chemically modified or not and a Protein or a peptide; said protein being collagen, elastin, keratin, fibronectin, actin, myosin, laminin, a peptide or a blend of those proteins or peptides, fibrillated, hydrolysed, chemically modified or not. This homogenous composition is obtained by the polymerisation or the covalent bounding of two preparations containing cyclic polycarbonates, polyamines, glycosaminoglycans, hydrolysed glycosaminoglycans, glycosamines, chemically modified or not and proteins or peptides or a blend of those proteins or peptides, fibrillated, hydrolysed, chemically modified or not.

The invention discloses gene of codon optimized N-acetyl glucosamine isomerase and the expression thereof, and the nucleotide sequence of the gene is shown as SEQ ID NO:1. The gene has the advantages that anAGE gene is optimized and synthesized according to Pichia pastoris codon preference, the homology of the optimized anAGE gene is 79 percent of that of a wild gene, the enzyme activity of 6.29 U / mg crude enzyme is shown, and the gene can be applied to the synthesis of N-acetylmannosamine.

The present invention relates to compositions comprising chondroitin sulphate and mannosamine or a derivative thereof. The mannosamine derivative is preferably N-acetylmannosamine. The compositions may comprise glucosamine. Said compositions are useful in the treatment or prevention of degenerative joint diseases, preferably of osteoarthritis, in the treatment or prevention of tendon or ligament diseases, disorders or injuries and of immune system diseases, preferably of rheumatoid arthritis.

The invention relates to an elastic renewable polyester and a preparation method thereof. The elastic renewable polyester is a polyester copolymer containing isosorbitol, glycosamine and other renewable compounds, and m-benzenedicarbonyl and other monomers. The preparation method mainly comprises the following steps: proportionally adding dimethyl terephthalate, dimethyl isophthalate, ethanediol, propanediol, isosorbitol, glycosamine and other monomers and a catalyst into a reaction bulb in a nitrogen protective atmosphere, carrying out programmed heating, and reacting while lowering the pressure according to needs; and after reacting for some time, cooling to room temperature, slowly restoring to atmospheric pressure, discharging in the nitrogen protective atmosphere, washing, filtering, and carrying out vacuum drying to obtain the finished product. The obtained polyester has the advantages of favorable hydroscopic property and favorable elasticity, and is renewable, degradable, green and environment-friendly.

The present invention relates to a process for the synthesis of 6-azido-6-deoxy-2-N-acetyl-monosaccharide-nucleoside diphosphate, in particular 6-azido-6-deoxy-2-N-acetyl-D-galactosamine-nucleoside diphosphate or 6-azido-6-deoxy-2-N-acetyl-D-glucosamine-nucleoside diphosphate, in particular to a process for the synthesis of 6-azido-6-deoxy-2-N-acetyl-D-galactosamine-nucleoside diphosphate or 6-azido-6-deoxy-2-N-acetyl-D-glucosamine-nucleoside diphosphate. The synthesis method according to the invention is characterized by high efficiency and high yield. The other part of the invention is a key intermediate of the method.

The present invention relates to new generation of gentamicin and its preparation process and use. It is prepared via adding glutamic acid, sodium glutamate or glutamic amine into the conventional deep red micromonad fermenting culture medium comprising corn powder, starch, yeast powder or soybean powder, potassium nitrate, calcium carbonate, ammonium sulfate, peptone and cobalt chloride, one or several kinds of additives are added selected from: rice powder, millet powder, fructose, glycosamine, ferric gluconate, zinc chloride, ferrous citrate, ferrous lactate, vitamin B12, etc. The gentamicin of the present invention, gentamicin C1, has the advantages of high gentamicin C1 content and less toxic side effect. The product of the present invention is one kind of antibiotic for human and animal.

The invention discloses an amphoteric glycolipid biosurfactant and a preparation method thereof, belonging to the technical field of biomaterials. Among them, the amphoteric glycolipid biosurfactant includes the following components: fatty acid, polysaccharide, amphoteric glycolipid biosurfactant molecule, sugar amine, lipopeptide, ethanol and fatty amine, etc. Wherein the preparation method of the amphoteric glycolipid biosurfactant is: (1) cultivation: adopt three kinds of bacteria to carry out stage-by-stage cultivation; (2) separation: adjust the pH value of the fermentation broth, and centrifuge and reclaim the clear liquid in the middle layer; (3) ) acid precipitation: adjust the pH value of the clear liquid in the middle layer, and then refrigerate and precipitate; (4) filter: collect the precipitate with a ceramic membrane, then dissolve the precipitate, and filter it again to obtain an aqueous solution; (5) concentrate: concentrate the aqueous solution in a vacuum, Obtain a concentrated aqueous solution; (6) finished product: add the above-mentioned components to the concentrated aqueous solution and stir. The prepared amphoteric glycolipid biosurfactant is a high-quality amphoteric glycolipid biosurfactant.

The invention discloses a kit for simple and rapid detection of glycosaminoglycans, which comprises the following components: DMB reagent; chondroitin sulfate standard product; lysate; dissociation solution. In addition, the invention also discloses a detection method of the kit. The kit of the present invention directly combines the DMB dye (reagent) with glycosaminoglycan (GAG). After the dye is dissociated, it detects the absorbance value at a specific wavelength of the dissociated dye of the sample to be tested and the standard product, and directly quantitatively detects the glycosaminoglycan. polysaccharide content. The kit of the invention greatly reduces the experimental cost and shortens the detection time, and at the same time has no selectivity for the species of samples such as tissue cells, and is easy to operate. It can detect the level of glycosaminoglycans in various human or animal tissues (skin, muscle, etc.), cartilage, connective tissue, tumor tissue, body fluid, cell culture supernatant and other samples.

A composition comprising Polyhydroxyurethanes, Glycosaminoglycans, hydrolysed glycosaminoglycans, glycosamines of glycosaminoglycans, chemically modified or not and a Protein or a peptide; said protein being collagen, elastin, keratin, fibronectin, actin, myosin, laminin, a peptide or a blend of those proteins or peptides, fibrillated, hydrolysed, chemically modified or not. This homogenous composition is obtained by the polymerisation or the covalent bounding of two preparations containing cyclic polycarbonates, polyamines, glycosaminoglycans, hydrolysed glycosaminoglycans, glycosamines, chemically modified or not and proteins or peptides or a blend of those proteins or peptides, fibrillated, hydrolysed, chemically modified or not.

The invention discloses a preparation method of N-acetyl-D-galactosamine. The preparation method comprises the following steps that S1, acetylation is carried out on D-galactosamine hydrochloride to prepare D-galactosamine pentaacetate; and S2, O-acetylation is carried out on the glycosamine pentaacetate to generate N-acetyl-D-galactosamine. A reaction system in the S1 contains D-galactosamine hydrochloride, a solvent, an acylating agent and an acid-binding agent, the acylating agent is acetic anhydride, the acid-binding agent is prepared from 4-dimethylaminopyridine, and the reaction temperature is -5 DEG C to 5 DEG C; and the solvent is prepared from organic bases with pKa of 5-12 at 25 DEG C. According to the preparation method of the N-acetyl-D-galactosamine, the alkaline solvent firstly reacts with HCl in the D-galactosamine hydrochloride, then the alkaline solvent and the 4-dimethylaminopyridine jointly promote acetylation, the yield of the peracetylation reaction of the D-galactosamine hydrochloride is increased, the reaction rate is increased, the peracetylation reaction conditions are mild, and production is easy to expand.