11 results about "Glycosamine" patented technology

The current invention concerns methods for the synthesis of 6-azido-6-deoxy-2-N-acetyl-monosaccharide-nucleoside diphosphate, in particular 6-azido-6-deoxy-2-N-acetyl-D-galactosamine-nucleoside diphosphate or 6-azido-6-deoxy-2-N-acetyl-D-glucosamine-nucleoside diphosphate. The synthesis method according to the invention is characterized by being highly efficient and high yielding. Also part of the present invention are key intermediates of this process.

This application relates to the technical field of vaccine quality testing, specifically to a quantitative detection method for 3D-MLA in liposome adjuvants. The method includes the following steps: taking a test sample solution, adding hydrochloric acid for acid hydrolysis to completely hydrolyze 3D-MLA into D-glucosamine and D-glucosamine-6-phosphate; adding an internal standard solution and a derivatizing reagent to the hydrolyzed sample to perform a derivatization reaction, converting the glycosamine and internal standard into fluorescent derivatives; detecting the derivatized sample using high-performance liquid chromatography-fluorescence detector; and calculating the 3D-MLA content in the test sample using a standard curve method based on the ratio of the total peak area of ​​glucosamine to the peak area of ​​the internal standard. The detection method provided in this application is highly specific, sensitive, easy to operate, and suitable for large batches of samples.

The present invention relates to a method for the synthesis of 6-azido-6-deoxy-2-N-acetyl-monosaccharide-nucleotide diphosphates, in particular 6-azido-6-deoxy-2-N-acetyl-D-galactosamine-nucleotide diphosphate or 6-azido-6-deoxy-2-N-acetyl-D-glucosamine-nucleotide diphosphate. The synthesis method according to the present invention is characterized by high efficiency and high yield. Another part of the present invention is a key intermediate of the method.

The invention relates to a method for producing hyaluronic acid through cell-free catalysis, and belongs to the technical field of biological catalysis. According to the invention, a system capable of being applied to cell-free catalytic production of hyaluronic acid is constructed, and genetically engineered bacteria are used for expressing N-acetyl hexosamine 1-kinase, glucosamine-1-phosphate acetyl transferase, glucuronide kinase, GlcA-1-P uridinetransferase, hyaluronic acid synthase, polyphosphate kinase, uridine monophosphate kinase and inorganic pyrophosphatase; according to the present invention, N-acetylglucosamine GlcNAc, glucuronic acid GlcA, ATP and UMP are adopted as substrates to perform catalytic production, UDP and ADP produced during the reaction process are converted into ATP and UTP, such that nucleotide circulation is achieved, raw material consumption is reduced, the final hyaluronic acid yield reaches 1.28 g / L, and an economic and efficient brand new path is provided for hyaluronic acid industrial production.

This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, the disclosure is in the technical field of fermentation of metabolically engineered microorganisms. The disclosure describes engineered microorganisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These microorganisms can dephosphorylate N-acetylglucosamine-6-phopshate to N-acetyl glucosamine and convert the N-acetylglucosamine to N-acetylmannosamine. These microorganisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate. Furthermore, provided is a method for the large scale in vivo synthesis of sialylated compounds, by culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as, but not limited to lactose, lactoNbiose, N-acetyllactosamine and / or an aglycon, wherein the microorganism intracellularly dephosphorylates N-acetylglucosamine-6-phopshate to N-acetylglucosamine, converts N-acetylglucosamine to N-acetylmannosamine and convert the latter further to N-acetyl-neuraminate.

The invention discloses a functional composition for treating or preventing bone and joint diseases, a conditioner and application. The functional composition comprises a first active component and a second active component, and according to the concentration of the functional composition, the first active component comprises 1 [mu] g / mL-500 [mu] g / mL of elastin peptide, 40 ng / mL-20 [mu] g / mL of haematococcus pluvialis, 8 ng / mL-4 [mu] g / mL of emblic leafflower fruit and 1 [mu] g / mL-500 [mu] g / mL of mussel; the second active component comprises a broccoli aqueous extract and / or boric acid substances. Through scientific compatibility, all the components synergistically improve the levels of glycosamine poly and osteocalcin, the health of joints, cartilages and bones is protected, the components are natural in source, safe, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of bone diseases.

The invention discloses a plant extract for adsorbing swine mycotoxin and a feed, and belongs to the technical field of feed additives, and the plant extract for adsorbing swine mycotoxin comprises the following raw materials in parts by weight: grape pomace, silybum marianum straw, soybean peptide powder, chitosan oligosaccharide and rice hull source biochar. The preparation method comprises the following steps: inducing plant polyphenol, soybean peptide and chitosan oligosaccharide to generate mild glycosamine condensation and cross-linking reaction under a hydrothermal condition, reacting carbonyl of the chitosan oligosaccharide with polypeptide or amino of the chitosan oligosaccharide to form Schiff base, and further rearranging and cross-linking; meanwhile, a large number of phenolic hydroxyl groups, peptide bonds, hydroxyl groups and the like form a three-dimensional network structure through hydrogen bonds, and the three-dimensional network structure is attached to the surface of the rice hull source biochar at high temperature, so that mycotoxin can be adsorbed, and active substances with liver protection and oxidation resistance functions, such as silibinin and anthocyanin, can be slowly released; and antioxidant repair and protection are provided for intestinal tracts while the mould is adsorbed.

The invention relates to the technical field of functional food, in particular to a tendon-nourishing and bone-strengthening composition and a preparation method thereof, and the tendon-nourishing and bone-strengthening composition comprises a fermented mulberry leaf polyphenol-sea cucumber glycosamine polymer, clover leaf saponin, a selenium yeast-pachymaran compound, glucosamine, compound nuts, vitamin D, a beef-pork compound, a traditional Chinese medicine compound and cheese; the three functional components of the fermented mulberry leaf polyphenol-sea cucumber glycosamine polymer, the clover leaf saponin and the selenium yeast-pachymaran compound cooperate to effectively block the accumulation and toxic effect of harmful heavy metals in the Chinese herbal medicines in the human body, and normal absorption of trace elements necessary for the human body is not interfered at the same time.

This invention relates to a method for synthesizing 6-azido-6-deoxy-2-N-acetyl-monosaccharide-nucleoside diphosphate, particularly 6-azido-6-deoxy-2-N-acetyl-D-galactosamine-nucleoside diphosphate or 6-azido-6-deoxy-2-N-acetyl-D-glucosamine-nucleoside diphosphate. The synthetic method according to the invention is characterized by high efficiency and high yield. Another part of the invention concerns the key intermediates of this method.

This invention relates to the application of N-acetylmnosamine in pharmaceutical preparation, belonging to the field of biomedical technology. This invention provides the application of N-acetylmnosamine in the preparation of drugs for treating or preventing neurological dysfunction caused by hydrocephalus; in the preparation of drugs for treating or preventing motor disorders caused by hydrocephalus or neurodegenerative diseases; in the preparation of drugs for improving cognitive impairment; in the preparation of drugs for inhibiting astrocyte proliferation and promoting astrocyte transformation; and in the preparation of drugs for reducing white matter demyelination damage in brain tissue. Through the application of ManNAc provided by this invention, new drugs will be provided for the prevention and treatment of neurological dysfunction caused by hydrocephalus, offering new treatment directions for patients.

Provided are skincare compositions comprising: a compound having retinol-like activity and an N-acetyl compound selected from the group consisting of N-aldosamines, N-acetylated amino acids and combinations thereof. Also provided are methods for treating skin comprising topically applying these compositions. Also provided are skincare compositions comprising 3-(4- farnesyloxyphenyl)-propionic acid; and a derivative of an aminosugar selected from the group consisting of N-acetyl-ribosamine, N-acetyl-arabinosamine, N-acetyl-glucosamine, N-acetyl- galactosamine and N-acetyl-mannosamine, and combinations thereof, wherein the composition is in the form of a solution, suspension, emulsion, lotion, cream, serum, gel, stick, spray, ointment, liquid wash, soap bar, shampoo, hair conditioner, paste, foam, powder, mousse, shaving cream, hydrogel, or film-forming product.