29 results about "Silibinin" patented technology

The invention discloses a composition capable of quickly dispelling the effects of alcohol and relieving moderate and mild fatty liver and application of the composition, and particularly relates to the technical field of health care products. The composition comprises the silybum marianum extract, the schisandra chinensis extract, the globe artichoke extract and the 1, 6-fructose diphosphate and cyclodextrin-clathrated silybum marianum extract as raw materials, on one hand, the bioavailability of the product is improved, the taste of the product is better, and the taste of the clathrated product is improved due to the fact that silybin is very bitter and cyclodextrin has a shielding property; and on the other hand, the FDP and the three plant extracts have a synergistic effect, and the combined composition can achieve the effects of quickly dispelling the effects of alcohol and relieving moderate and mild fatty liver.

The invention belongs to the technical field of biology, and discloses application of DVM nanoparticles in preparation of products for treating and / or preventing alcoholic liver diseases, the DVM nanoparticles contain gallic acid derivatives VM and dihydromyricetin; the mass ratio of the gallic acid derivative VM to the dihydromyricetin is 1: 3; in alcohol-induced HepG2 cells, the DVM nanoparticles can better reduce apoptosis of hepatocytes and generation of ROS, improve ADH activity, reduce AST and ALT activities in the cells and improve the hangover alleviating ability in mice, and have a more excellent liver protection effect compared with gallic acid and a positive drug silibinin.

The application discloses a plant extraction compound for reducing blood pressure, a preparation method and application thereof. The compound is composed of high-purity silybin extract and a specific fusion polypeptide through molecular self-assembly. The sequence of the fusion polypeptide is shown in sequence SEQ ID NO:1, which innovatively integrates cell membrane penetration function, angiotensin II receptor 1 antagonism function and hydrophobic drug binding function. The core of the application is that through polypeptide-mediated active targeting, silybin is accurately delivered to the diseased blood vessel area, and the double synergistic mechanism of upstream ACE inhibition and downstream receptor blocking of the renin-angiotensin-aldosterone system is realized. In-vivo pharmacodynamic experiments prove that compared with single components, the compound can produce more significant and persistent antihypertensive effect, and can effectively reverse myocardial hypertrophy and fibrosis caused by hypertension, and show excellent synergistic therapeutic effect and target organ protection ability.

The application discloses a capsule for assisting in protecting chemical liver injury and a preparation method thereof, and belongs to the technical field of health-care food. The capsule is composed of curcuma longa extract 100g, silybum marianum extract 70g, schisandra chinensis extract 60g, microcrystalline cellulose 109.5g, silicon dioxide 7g and magnesium stearate 3.5g, and 1000 capsules are taken as a unit. The curcuma longa extract contains curcumin, the content of which is greater than or equal to 10% (10:1), the silybum marianum extract contains silybin, the content of which is greater than or equal to 20% (25:1), and the schisandra chinensis extract contains schisandrin, the content of which is greater than or equal to 1.5% (12.5:1). The three kinds of plant extracts are scientifically compounded, and the effects of antioxidation, anti-inflammation, liver cell membrane protection and bile secretion regulation are synergistically exerted. Through systematic screening of the types and dosages of the filling agent, the flow agent and the lubricant, the technical problems of poor flowability of the mixed plant extract powder, large filling difference and easy jamming are solved.

The invention discloses a preparation method of a silybin nanostructure lipid carrier, which comprises the following steps: step 1, heating ethanol and silybin in a water bath, and uniformly stirring to obtain a silybin solution; dispersing a co-emulsifier in deionized water, and heating and stirring in a water bath to obtain a water phase; step 2, adding solid lipid, liquid lipid, a main emulsifier and a co-emulsifier into the silibinin solution, heating for melting, and uniformly stirring to obtain an oil phase; under the condition of constant-temperature stirring, transferring the oil phase into the water phase, mixing the solution, and shearing to obtain a coarse dispersion system; and 3, heating the coarse dispersion system to concentrate the coarse dispersion system into an emulsion. The silibinin nanostructure lipid carrier prepared by the invention can improve the solubility of silibinin, and is good in characterization performance; the preparation cost is controllable, and macro preparation can be realized; the silybin nano-structure lipid carrier can be used for effectively preventing acute liver injury caused by CCl4 of animals.

The invention relates to a silibinin derivative as well as a preparation method and application thereof, and belongs to the technical field of chemistry. The invention relates to a silibinin derivative, which is 6-sulfonated silibinin sodium sulfonate, the chemical name of the silibinin derivative is 2, 3-dihydro-3-(4-hydroxy-3-methoxyphenyl)-2-hydroxymethyl-6-(3, 5, 7-trihydroxy-4-oxo benzopyran-2-yl) benzodioxane-6-sodium sulfonate, the molecular formula of the silibinin derivative is C25H21NaO13S, and the molecular weight of the silibinin derivative is 584.49. Sulfonic acid groups with hydrophilicity are introduced into different parts of the silibinin to prepare different types of silibinin sodium sulfonate, so that the solubility of the silibinin sodium sulfonate in water is enhanced, the pharmacokinetic characteristics of the silibinin are improved from the pharmacokinetic level, the affinity and biological activity of the silibinin to organisms are improved, and the application prospect of the silibinin sodium sulfonate is widened. The compound is further prepared into an injection for clinical treatment, and has important clinical drug treatment value.

The invention relates to the technical field of medicine, in particular to a recombinant human XVII type collagen / silibinin nanoemulsion and a preparation method and application thereof, and the nanoemulsion is prepared from the following components in percentage by weight: 0.2%-1.5% of recombinant human XVII type collagen, 0.5% of silibinin, 1%-10% of lecithin, 1%-10% of glycerol, 3%-15% of Tween 80, 2%-8% of PEG400 and the balance of pure water. The prepared nano-emulsion is uniform in size and high in stability, irritation is effectively reduced, skin light damage can be remarkably repaired, the synergistic effect of oxidation resistance and barrier repair is achieved, UVB-induced oxidative stress, inflammatory response and skin structure damage are inhibited in multiple ways, and the nano-emulsion has a good prospect in application of skin UVB light damage repair.

The invention discloses a health-care product composition for blood purification and liver and gall detoxification as well as a preparation method and application thereof, and belongs to the technical field of health-care products. The health-care product composition for blood purification and liver and gall detoxification provided by the invention is prepared from the following components in parts by mass: 10 to 40 parts of fermented barley leaf powder, 5 to 20 parts of a silibinin phospholipid complex, 5 to 15 parts of a dandelion extract, 5 to 15 parts of an artichoke extract, 5 to 10 parts of curcumin, 2 to 8 parts of alpha-lipoic acid and 1 to 5 parts of N-acetylcysteine. According to the composition, a four-dimensional synergistic purification system of intestinal adsorption purification, liver biochemical detoxification, bile excretion promotion and whole-course antioxidant protection is constructed, and the components are mutually promoted and enhanced at different time and space and different target spots to synergistically achieve the double effects of blood purification and liver and gallbladder detoxification.

The invention belongs to the technical field of pharmaceutical compositions, and particularly relates to a composition for improving the bioavailability of silibinin, a preparation method and application. Highly-dispersed micro-powder particles are formed by mixing the silybum marianum extract and the specific plant extracts (the liquorice extract, the radix puerariae extract and / or the gynostemma pentaphylla extract) according to a specific proportion and carrying out compressed air suspension, dispersion and granulation, and on the premise that an organic solvent and a surface active agent are not added, the micro-powder particles can be uniformly dispersed, so that the preparation process is simplified, and the preparation cost is reduced. According to the silybum marianum extract, the dissolution rate and bioavailability of silybin in the silybum marianum extract are remarkably improved, the label is clean, the preparation method is simple, and the silybum marianum extract has a good application prospect.

This invention discloses the use of silybin derivatives or their stereoisomers, or pharmaceutically acceptable salts, with the structure shown in Formula I in the preparation of medicaments for the prevention and / or treatment of depressive disorders; R1, R2, R3, R4, R5, and R6 are independently selected from hydrogen, methyl, and methyl groups. Compared with silybin alone, royal jelly acid, or a combination of silybin and royal jelly acid, the silybin derivatives of this invention exhibit more significant effects on mouse models of depression induced by chronic social frustration stress and chronic restraint stress, even with significantly reduced dosage, and can be used to prepare medicaments for the treatment of depressive disorders.

The invention discloses an atractydin dispersion solubilizing method based on a deep neural network and strategy optimization, and aims to solve the problems that key quality attributes in online monitoring and closed-loop control in the preparation process are difficult to stably reach the standard, and batch difference and equipment safety are difficult to consider at the same time. According to the invention, uncertainty prediction is carried out by combining a multi-mode cross-attention depth model with energy sensing position coding and sensor reliability weighting and controlling affine and a Shenchang differential equation with physical constraints, and self-adaptive parameter adjustment of a rolling optimization driving actuator is predicted and controlled by a double-time-domain model of a probabilistic security constraint and control barrier function. The technical effects of online accurate prediction and stable standard reaching of key quality attributes (including the median diameter of the particle size, the polydispersity index, the electrokinetic potential and the dissolution curve), smooth action, energy consumption reduction and process and equipment safety control are achieved.

The invention discloses a silybin B molecular imprinting template as well as a preparation method and a use method thereof, and belongs to the technical field of molecular imprinting, and the preparation method comprises the following steps: adding silybin B, N-isopropylacrylamide, ethylene glycol dimethacrylate and polyvinylpyrrolidone into acetonitrile, uniformly dispersing, then adding azodiisobutyronitrile, and uniformly stirring to obtain the silybin B molecular imprinting template. The preparation method comprises the following steps: reacting under the protection of nitrogen, washing the reacted insoluble substance with a mixture of methanol and acetic acid, and drying to obtain polymer microspheres which are used as the silibinin B molecular imprinting template. Molecularly imprinted polymer microspheres are prepared through a free radical polymerization reaction, the formed microspheres are uniform in structure and not prone to agglomeration, the obtained polymer microspheres have the temperature-sensitive characteristic and can adsorb silybin B molecules at the room temperature, the polymer microspheres are subjected to phase transformation after being heated, the polymer structure shrinks, silybin B is released, and the silybin B can be effectively adsorbed. Silybin B molecules can be efficiently separated by utilizing selective adsorption and temperature-sensitive characteristic desorption of a molecular imprinting technology on the silybin B.

The invention provides a purification method of silymarin and a preparation method of high-purity silybin, and relates to the technical field of silybin. According to the method, one of the ethanol water solution, the isopropanol water solution and the ethanol-ethyl acetate solution is used as the purification solvent for purifying the silymarin, so that a silybin crude product with relatively high purity can be obtained. And dissolving the silybin crude product with hot isopropanol, concentrating, adding hot water, and precipitating to separate out the silybin, thereby obtaining the high-purity silybin with the purity not lower than 99%. The method is simple and suitable for industrial production.

The present invention relates to a silybin derivative, a preparation method therefor and the use thereof, belonging to the technical field of chemistry. In the present invention, by means of introducing a hydrophilic sodium sulfonate group into silybin, silybin is sulfonated to prepare a new compound having a good water solubility, i.e. sodium silybin sulfonate. This increases the solubility of silybin in water, improves the pharmacokinetic characteristics of silybin from a pharmacokinetic perspective, and increases the affinity therewith for organisms and the bioactivity of silybin. The compound can be formulated into an injection for use in clinical treatment, providing a better administration route. Sodium silybin sulfonate has incomparable advantages over silybin, and thus also has broad significance and great therapeutic value in clinical applications.

The invention relates to the field of medical instruments, the field of medical or dressing preparations, the technical field of biological medicine manufacturing and the technical field of preparation, in particular to a supramolecular anti-aging composition suitable for people with sensitive skin and a preparation method and application of the supramolecular anti-aging composition. Active ingredients, an inclusion material, an emulsifier and a solvent; the active ingredients are prepared from silymarin, silanetriol and phytol; the silymarin comprises silibinin, silymarin and silymarin; the silymarin comprises silybin, silymarin and silymarin; the inclusion material comprises at least one of hydroxypropyl-alpha-cyclodextrin and hydroxypropyl-beta-cyclodextrin, and the inclusion material comprises at least one of hydroxypropyl-alpha-cyclodextrin and hydroxypropyl-beta-cyclodextrin; the emulsifying agent is prepared from at least one of oleyl alcohol polyether-20, Tween 80 and PPG-13-decyl tetradecyl alcohol polyether-24; the solvent comprises water and polyhydric alcohol. The composition provided by the invention has excellent anti-aging effect and stability, has a good effect of reducing allergy, and is suitable for sensitive people.

The invention discloses an application of quercetin and silibinin in preparation of a medicine for promoting liver regeneration. Mouse partial liver resection model experiments prove that quercetin and quercetin and silybin combined medication can remarkably improve the liver weight ratio, liver cell proliferation is further promoted by activating a prostaglandin D2 receptor 1, especially liver regeneration after partial liver resection is promoted, and the effects of repairing liver injury and promoting liver proliferation are achieved.

The invention discloses a blood fat reducing diglyceride composition and a preparation method and application thereof, and relates to the technical field of biological medicine, the blood fat reducing diglyceride composition comprises the following components: 30%-60% of diglyceride, 30%-55% of a structured matrix, 2%-8% of phytosterol, 1%-6% of an auxiliary functional composition, and the balance of water. According to the invention, a novel water-dispersible solid particle system is successfully constructed, and particularly rosmarinic acid is introduced as a natural efficient antioxidant to form an'internal and external synergistic 'antioxidant network with silibinin, so that the oxidation of diglyceride is obviously delayed, and meanwhile, the stability of the system in an intestinal environment is enhanced. The rosmarinic acid and the fucoidan can form a composite layer on a colloid interface, so that the physical stability and the slow release characteristic of the nano colloid are further improved.

The invention belongs to the technical field of medicines, and relates to application of a silybin derivative in light damage protection, and the structural formula of the silybin derivative is shown in the specification. The silybin derivative has the functions of broad-spectrum physical sunscreen and chemical sunscreen, the physical sunscreen comprises the broad-spectrum absorption characteristic, and the ultraviolet photon absorption capacity is remarkably improved. The chemical sunscreen has the effects of resisting inflammation and promoting DNA repair. The silybin derivative can effectively prevent and treat acute skin light injury caused by ultraviolet radiation, and has wide development and application prospects.

The application belongs to the technical field of biology, and discloses application of gallic acid derivatives in preparation of products for treating or preventing liver diseases, wherein the gallic acid derivatives are one or more combinations of 2-Phenylethyl 1-O-beta-D-(6'-O-galloyl)-glucopyranoside, 2,3-digalloyl-D-glucopyranose, Kaempferol 3-O-glucoside-2"-gallate and Quercetin 3-O-(6'-O-Galloyl)-beta-galactoside; the four gallic acid derivatives can better reduce AST and ALT activities in alcohol-induced HepG2 cells relative to a model group, and 2,3-digalloyl-D-glucopyranose and Quercetin 3-O-(6'-O-Galloyl)-beta-galactoside have better effects than gallic acid and positive drug Silybin (Silybin).

Embodiments of the present disclosure disclose a use of a composition in the preparation of a retinoic acid X receptor gamma activator, the composition comprising phytol, teprenone, and silybin. Embodiments of the present disclosure also disclose a use of the composition in the preparation of an extracellular matrix metalloproteinase inhibitor and an interleukin-6 inhibitor at a specific ratio.

The invention discloses a culture solution of Vdelta2T cells as well as a culture method and application of the culture solution. The culture solution comprises an activation culture medium and an amplification culture medium, and a serum-free culture medium is taken as a basic solution; calcium chloride, IL-2, IL-15, IL-21, zoledronic acid, L-glutamine, beta-mercaptoethanol, N-acetylcysteine, human AB serum and silibinin are added into the serum-free culture medium of the activated culture medium; iL-2, human AB serum and hyperoside are added and contained in a serum-free culture medium of the amplification culture medium. According to the culture solution of the Vdelta2T cells and the multiplication culture method for culturing the Vdelta2T cells by using the culture solution, the in-vitro amplification capacity (the amplification multiple is as high as 7416 times) of the Vdelta2T cells can be remarkably improved, the purity of amplified NK cells is as high as 99%, and the tumor cell killing power (the tumor killing effect is relatively good when the ratio of E to T is 1: 1) can be remarkably improved.

The invention provides a method for simultaneously determining silibinin and carvedilol in plasma by a positive and negative ion switching technology. The specific operation of the method comprises the following steps: adding an internal standard solution and an organic solvent into a plasma sample, and ensuring full extraction of silibinin and carvedilol through vortex mixing. Separating by utilizing a centrifugal technology, collecting supernate, diluting and taking the supernate as a pretreated to-be-detected sample; the method comprises the following steps: carrying out gradient elution by adopting a Scepter Phenyl-120 chromatographic column to realize effective separation of silibinin and carvedilol, and then carrying out mass spectrometric detection on a sample. A standard curve is drawn based on the mass spectrum peak area ratio, a regression equation is obtained, and the concentration of silibinin and carvedilol in the to-be-detected sample is obtained through calculation. The method has the characteristics of simplicity and convenience in pretreatment operation, low plasma consumption, short analysis time and high sensitivity, and is suitable for detection of silibinin and carvedilol in plasma and pharmacokinetic research of silibinin and carvedilol.

Chinese herbal medicine combination for alcohol detoxification and liver protection, characterized in that the Chinese herbal medicine combination comprises the following components: Milk thistle extract, kudzu extract, astragalus extract, and salvia extract; the mass ratio of puerarin to silybin contained in the Chinese drug combination is 1:4 to 4:1.

The present application relates to the field of medical devices, the field of medical or toiletry formulations, the field of biopharmaceutical manufacturing technology and the field of formulation manufacturing technology, in particular to a supramolecular anti-aging composition suitable for sensitive skin population and a preparation method and application thereof. Active ingredients, inclusion materials, emulsifiers and solvents; the active ingredients include: silymarin, silatrane and phytol; the silymarin includes silybin, silychristin and silydianin; the inclusion material includes at least one of hydroxypropyl-alpha-cyclodextrin and hydroxypropyl-beta-cyclodextrin; the emulsifier includes at least one of oleyl polyether-20, Tween 80 and PPG-13-decyltetradecyl alcohol polyether-24; the solvent includes water and polyhydric alcohol. The composition provided by the present application has excellent anti-aging efficacy and stability, and has good effect of reducing allergy, and is suitable for sensitive population.

The invention discloses an application of silybin in treating androgenetic alopecia. Silybin is a main active component extracted from Silybum marianum, and the component has a remarkable effect in the aspects of liver protection and oxidation resistance. Experiments show that when a mouse is taken as an object, a silibinin group can quickly promote hair growth. The silybin can be used as a unique active component and is used for preparing a medicine or a reagent special for androgenetic alopecia and promoting hair growth. Silybin is high in safety and small in toxic and side effects, has extremely wide application prospects in the fields of prevention, nursing and treatment of the problems of alopecia and development of pharmaceutical preparations, cosmetics and the like for promoting healthy growth of hair, and is expected to bring new breakthrough and development to related fields.

The invention relates to a silibinin derivative as well as a preparation method and application thereof, and belongs to the technical field of chemistry. The silibinin derivative is characterized in that the chemical structure of 22-sulfonated silibinin sodium sulfonate of the derivative is as shown in a general formula (II), and the chemical structure of 21-sulfonated silibinin sodium sulfonate is as shown in a general formula (III). The invention has the beneficial effects that the water solubility is obviously improved, the bioavailability is greatly improved, the pharmacodynamic advantage is prominent, the dosage form is excellent, the stability is good, and the selectivity of the preparation process is good; the sulfonation modification strategy is also suitable for structural optimization of other flavonoid lignan compounds (such as isosilybin, silymarin, silymarin and the like) with similar structures, and a new thought is provided for development of similar high-solubility drugs.

The invention provides application of silybin and chenodeoxycholic acid in improving the quality reduction of procambarus clarkia caused by zero-fish-meal feed, and belongs to the technical field of aquatic feed additive development. According to the present invention, silibinin and chenodeoxycholic acid are combined according to a mass ratio of (1-2): (1-2) so as to effectively improve the reduction of the quality of the procambarus clarkia caused by the zero fish meal feed, such that the zero fish meal use is achieved, and the procambarus clarkia breeding method is suitable for the procambarus clarkia breeding of procambarus clarkia breeding of procambarus clarkia breeding of procambarus clarkia breeding of procambarus clarkia. The feed cost can be effectively reduced, and the muscle quality of the procambarus clarkii cannot be reduced.

This invention discloses a natural plant-based liver-protecting preparation based on promoting bile secretion and its preparation, relating to the fields of natural plant medicine and health food technology. This invention effectively achieves liver protection through the synergistic effects of promoting bile secretion, choleretic detoxification, and direct protection of hepatocytes. Specifically, milk thistle extract, rich in silybin, can stabilize hepatocyte membranes, prevent toxic substances from entering hepatocytes, and promote the repair and regeneration of damaged hepatocytes; cynarin in artichoke extract accelerates the excretion of toxins and waste products by promoting bile secretion, reducing the burden on the liver; and the total flavonoids in dandelion root extract have anti-inflammatory and antioxidant effects, protecting hepatocytes from free radical damage, thereby comprehensively improving liver health.

The invention provides a probiotic compound preparation with double effects of short-term hangover alleviating and long-term liver protection. The silybum marianum and hovenia dulcis thunb compound is fermented through the bifidobacterium animalis subsp. Animalis HX-BA21, and the concentration of the anti-inflammatory protein P75, the dihydromyricetin and the silybin which serve as core functional components is remarkably increased; and further compounding with corn oligopeptide, glutathione yeast and turmeric to construct a multi-channel synergistic system of anti-inflammation, metabolism acceleration, oxidation resistance, hepatocyte repair and lipid metabolism regulation. On one hand, alcohol metabolism is accelerated, the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) is improved, and rapid alcohol effect dispelling is achieved; on the other hand, the antioxidant capacity is enhanced (SOD and GSH-Px are remarkably improved, and MDA is reduced), intestinal tract-derived inflammation signals are synergistically inhibited (IL-6 and TNF-alpha are reduced), hepatocytes are synergistically repaired, and lipid metabolism is regulated (TC and TG of the liver and ALT and AST of serum are reduced) through P75 protein, so that long-term protection and hepatocyte repair of alcoholic liver injury are realized while the acute drunkenness symptom is relieved. The method has the advantages of high efficiency, safety and multi-target intervention, and is obviously superior to a single component or a simple physical mixture.