140results about How to "Conducive to mass production operations" patented technology

The invention relates to a process for preparing hesperidin, which has simple and convenient operation and less pollution and equipment investment. The process comprises the following steps: adding 3to 10 times of 10-80 percent of alcohol into dried orange peel coarse powder to heat, back flow and extract for one to three times for 2 hours each time; combining extracting liquid and filtering; decompressing filter liquid to recover the alcohol till completion; filtering when the filter liquid is hot, refrigerating for 12 hours and filtering; washing filter residues by water, drying at low temperature and adding 50 percent of alkaline alcohol to dissolve; filtering and adding acid into the filter liquid to neutralize; precipitating sediments and collecting the sediments; recrystallizing thealcohol, washing and drying to obtain the hesperidin. The process for preparing hesperidin has high product purity and is easy to realize industrialization amplification.

The invention relates to a method for preparing Silymarin. The method has the advantages that the operation is simple and convenient, the pollution is low and the equipment investment is small. The technological steps are that: Silybum marianum fine powder is taken and is added in a CO2 supercritical extractor, methanol is used as entrainer, the entrainer accounts for 2-6 percent of the total volume of extraction solvent, the extraction pressure is 10-40MPa, the extraction temperature is 30-60 DEG C, CO2 flow is 1-5ml per gram crude drug per minutes, the extraction time is 150-250min, the extract is obtained, acetone is added, agitation is conducted to enable the acetone to be dissolved, filtration is conducted, the filtered liquid is obtained and is concentrated, the obtain liquid is filled in a high-efficiency countercurrent extractor, water-acetone-chloroform (1:5:2) solvent system is used, the bottom phase is a fixed phase, the top phase is a flowing phase, Silymarin segments are collected and are concentrated, crystals are precipitated and are separated, and acetone-petroleum ether (0.5-2:1) are added for recrystallization. The invention has the advantages that the purity of the prepared Silymarin is high and the industrialization amplification can be realized easily.

The invention relates to a method for preparing gastrodine, which has simple operation, light pollution and little equipment input. The method has the following process steps: taking coarse gastrodine powder; adding 60 to 90 percent ethanol into the coarse powder for percolation; collecting percolate of which the volume is 5 to 10 times the weight of a medical material; filtering, and reclaiming the ethanol at reduced pressure; condensing the percolate to dryness; adding methanol into residue for dissolution; injecting the solution into a high-efficient countercurrent extractor; using a lower phase of a water-methanol-acetic ether (1:1:8) solvent system as a stationary phase and an upper phase as a mobile phase, and collecting and condensing a gastrodine section; precipitating and separating crystal; and adding methanol-acetic ether (1:5-10) into the crystal for recrystallization. The gastrodine prepared by the method has high purity and is easy to realize industrialized amplification.

The invention relates to a preparation method of huperzine A, which is characterized by simple operation and little pollution and comprises the following processing steps: taking huperzine serrate dry coarse powder, adding 2-30 times amount of acid solution for ultrasonic extraction, with power of 100-2000W and extraction time of 10-30min, filtering extracts by anion exchange resins, extracting liquid medicines by chloroform, combining chloroform layers, recovering the chloroform at reduced pressure and concentrating the chloroform, adding the chloroform to a macroporous resin absorption column, using water for elution and impurity removal, using 50-90% of ethanol for elution, collecting eluent, recovering the ethanol at reduced pressure, adding acetone-chloroform for crystallization, washing and drying the crystal, thus obtaining the huperzine A. By adopting the invention to prepare the huperzine A, the product is high in purity and industrialization enlargement is easy to realize.

The invention relates to a preparation method of dogwood polysaccharides. The process comprises the following steps of: taking dry residues obtained after extracting total glucoside from dogwood pulp alcohol as a raw material, adding 5-10 (V/W) times of warm water, ultrasonically extracting for 1-2 h, combining the extracting solutions, concentrating, adding 30% of H2O2 and 3% trichloroacetic acid, centrifuging, taking the supernate, ultrafiltering, firstly adding a right amount of water into entrapped matter, then adding different concentrations of alcohol for fractional precipitation, filtering out high concentration alcohol precipitates and drying to obtain the product. The process has the advantages of simple operation, lower cost and higher purity and is beneficial to large production operation.

The invention relates to a method for preparing catalpol, which has simple operation, light pollution and less equipment investment. The method comprises the following processing steps of: taking fresh roots of rehmannia root; adding methanol of which the volume is 5 to 10 times weight of the fresh roots; refluxing for 2 to 4 times, and taking 0.5 to 2 hours for each refluxing; merging backflow liquid; filtering the liquid; recovering the methanol at reduced pressure and condensing the liquid; adding isopyknic water saturation butarol to extract for 3 to 8 times; merging a butarol layer; recovering the butarol at reduced pressure until the butarol is exhausted; adsorbing the butarol with a macroporous adsorbent resin column; carrying out water washing to remove impurities; carrying out elution with 5 to 30 percent ethanol; collecting eluent of which the volume is 3 to 8 times volume of the column; concentrating and drying the eluent; adding the eluent to a silica gel chromatographic column by a dry method; carrying out elution with chloroform-methanol-water (6:4:1); making the eluent in each column volume as one fluid part; collecting 3rd to 6th fluid parts; merging the fluid parts; recovering the solvent and concentrating the fluid parts; and adding anhydrous ethanol to crystallize. The catalpol prepared by the method has high product purity and is easy to realize industrialization expansion.

The invention relates to a method for preparing atractylon, which has the characteristic of simple and convenient operation, light pollution and low energy consumption. The method comprises the following process steps of: selecting a dry rhizome of plants, such as atractylis lancea, atractylodes chinensis and rhizoma atractylodis macrocephalae, crushing the rhizome, and adding the rhizome into a CO2 super critical extractor for extracting to obtain an extract; injecting the extract into a molecular distillation apparatus, and then performing two-grade molecular distillation on the extract in the molecular distillation apparatus, wherein the temperature of an evaporating surface of a first grade distillation is between 60 and 90 DEG C, the pressure is 100 to 150Pa, and the distillation time is 40 to 80 minutes; collecting distillate, and then performing a second grade distillation on the distillate, wherein the temperature of the evaporating surface is between 30 and 50 DEG C, the pressure is 60 to 80Pa, and the distillation time is 40 to 80 minutes; and then collecting the distillate, adding acetone, refrigerating, crystallizing, separating a crystal, washing and drying the crystal to obtain the product. The atractylon prepared by the method has high purity, and the industrial amplification is easy to realize.

The invention relates to a method for preparing indirubin with simple and convenient operation, small pollution and little equipment investment. The method comprises the following technological steps:adding methanol with the quantity of 5-10 times of raw materials into the raw materials, extracting by ultrasonic for 1-3 times, extracting for 0.2-1 hour each time, merging extracting liquid, recovering the methanol by depressurization, concentrating, filtering liquor by a macroporous resin column, eluting with water firstly, removing impurities, then eluting with ethyl acetate, concentrating eluent, adopting chromatographic separation by an alumina column, eluting with chloroform-ethyl acetate, concentrating eluent, recrystallizing with methanol-acetone, washing, drying to obtain the indirubin. By adopting the invention to prepare the indirubin, the product purity is high and industrialization amplification is easy to realize.

The invention relates to a preparation method of colchicumautumnale, which is characterized by simple operation and little pollution and comprises the following processing steps: taking raw fine powder, soaking the fine powder in aqueous alkali solution overnight, drying the powder and placing the dried powder in a CO2 supercritical liquid extraction device for supercritical liquid extraction; adding 2% aqueous hydrochloric acid solution to extracts to dissolve the extracts, filtering the extracts, adding chloroform to filtrate for extraction, taking subnatant, adjusting pH value to 13, adding the chloroform for extraction, taking the chloroform layer, carrying out separation by a macroporous resin absorption column, taking aqueous acid as an eluting agent for elution, collecting eluent, concentrating the eluent, adding acetone for recrystallization, washing and drying the crystal, thus obtaining the colchicumautumnale. By adopting the invention to prepare the colchicumautumnale, the product is high in purity and industrialization enlargement is easy to realize.

The invention relates to a method for preparing naringin, which is simple in operation, light in pollution and low in equipment investment, and comprises the following process steps of: taking coarse powder of pummelo peel, adding water of which the volume is 8 to 12 times the weight of the coarse powder of pummelo peel into the coarse powder, soaking at the temperature of between 30 and 80 DEG C for 8 to 24h, performing filter pressing, taking filtrate, adding CaCl3 into the filtrate, uniformly stirring the filtrate added with CaCl3, standing the mixture overnight, filtering, taking the filtrate, adding strong ammonia water into the filtrate to adjust the pH to between 7 and 8, adsorbing the filtrate added with strong ammonia water with a macroporous adsorption resin column, eluting with water to remove impurities, eluting with 50 to 90 percent ethanol, collecting the eluate of which the volume is 3 to 8 times that of the column, reclaiming the ethanol at reduced pressure, concentrating the eluate, and refrigerating the eluate to separate out crystal. The naringin prepared by the method is high in purity and easy to realize industrial enlargement.

The invention relates to a preparation method of oleanolic acid. In the process, glossy privet fruit is taken as the raw material, a crude product is obtained through extraction for 2-3 times by oversaturated lime water with 2-3 hours for each time, liquid medicine combination, acidification by adding acid and filtration; backflow extraction is carried out on the crude product by 50-60% of methanol; and the product can be obtained through heated filtration, microfiltration and impurity removing of the filtrate, nanofiltration, concentration and drying. The process has the advantages of simple and convenient operation, little pollution and less equipment investment and is beneficial for large-scale operation.

The invention relates to a preparation method of alpha-mangostin and the method has simple operation, low pollution and less energy consumption. The method comprises the following process steps: taking the shells of Garcinia mangostana Linn. to grind, adding the grinded shells in a CO2 supercritical extractor, using ethanol as the entrainer to obtain an extract; adding the extract in a polyamide chromatography column, using a mixed solvent of dichloromethane and methanol in a volume ratio of 8:1 as the eluting agent to perform elution, performing matching detection by drop a part of eluent on a polyamide film and using the eluting agent for developing, collecting the fluid phase which has 4-4.5 of Rf on the polyamide film, recycling the solvent, crystallizing, separating the crystals, recrystallizing with toluene, separating the crystals, washing and drying. By adopting the method of the invention to prepare alpha-mangostin, the product purity is high and the industrial amplification production is easy to realize.

The invention relates to a method for preparing spreading hedyotis herb polysaccharide, which has the advantages of simple and convenient operation, low pollution and small energy consumption. The method comprises the following process steps: taking spreading hedyotis herb; adding distilled water into the spreading hedyotis herb; putting the mixture into a microwave extraction apparatus for extraction; merging extract liquid; filtering the combined extract liquid; adsorbing the filtrate by using macroporous adsorption resin; eluting the product by using the distilled water; performing ultrafiltration and concentration by using a polysulfone ultrafiltration membrane with the molecule interception between 5,000 and 50,000 Dalton; adding ethanol into concentrated solution to ensure that the alcohol content of the solution is up to 70 to 90 percent; standing the solution for 24 hours; and filtering the obtained solution, taking filter residue, washing the obtained product and drying to obtain the spreading hedyotis herb polysaccharide. By adopting the method for preparing the spreading hedyotis herb polysaccharide, the product has high purity and the industrial amplification is easy to be realized.

The invention relates to a method for preparing celastrine with simple and convenient operation, low pollution and low energy consumption. The method comprises the following steps of: taking celastraceae plant celastrus paniculatus root bark, crushing the root bark, adding methanol in an amount which is 4 to 5 times the volume of the root bark into the crushed root bark, putting the mixture into an ultrasonic extraction device to perform ultrasonic extraction for 1 to 3 times for 0.2 to 1 hour each time with the extraction power of 30 to 60KHZ, combining the extract, filtering the extract, reclaiming the methanol under reduced pressure, adding 0.05 to 0.15 mol/L alkali aqueous solution into the residue to dissolve the residue, filtering the solution, adjusting the pH value of the filtrate to between 3 and 5 by using diluted hydrochloric acid solution, standing and filtering the solution, taking the filter residue, adding the filer residue into an aluminum oxide chromatographic column, eluting the filter residue by using a methanol-chloroform mixed solvent in a volume ratio of 3 to 1, collecting eluent in an amount which is 2 to 4 times volume of the aluminum oxide chromatographic column, reclaiming the solvent under reduced pressure, concentrating the eluent, adding the concentrate into an ethanol-n-hexane mixed solvent in a volume ratio of 1 to 1 to perform crystallization, and separating the crystal to obtain the celastrine. The celastrine prepared by adopting the method has high purity, and is easy to realize industrialized amplification.

The invention relates to a preparation method of paris polyphylla saponin, which has simple operation, low pollution and less equipment investment; the processing steps are as follows: paris polyphylla root-stack coarse powder is taken out, 3-12 times of 20-90 percent of ethanol is added for carrying out reflux extraction for 1-5 times, each time is carried out for 1.5 hours, the extracting solution is mixed, the filtration is carried out, the ethanol is recycled and concentrated by compression, petroleum ether degreasing agents are added, water is added in residue for metering the volume with one time volume of medicinal materials, water saturation normal butanol with equal volume is added for carrying out extraction for 2-8 times, the normal butanol liquid is mixed, and is recycled and concentrated by compression, the residue is dissolved by adding 95 percent of ethanol, pyroacetic spirit is added for recrystallization, and the paris polyphylla saponin is obtained by filtration, washing and drying. By adopting the method to prepare the paris polyphylla saponin, the product purity is high, so as to be easy to realize industrialization enlargement.

The invention provides a rapid propagation method of astragalus membranaceus calluses and astragalus membranaceus suspension cells. The rapid propagation method comprises the steps of obtaining a sterile explant, inducing calluses, culturing the suspension cells, regulating and controlling the activity of an enzyme in the suspension cells by using rare earth and the content of flavone saponins, and the like. The astragalus membranaceus suspension cells prepared by the method provided by the invention have high content of astragalus membranaceus saponins. The method provided by the invention realizes goal-directed regulation and control on the astragalus membranaceus suspension cells, thus a large amount of pharmaceutical raw materials can be obtained in short term and the production cost is effectively reduced.

The invention relates to a preparation method of galanthamine, which is characterized by simple operation and little pollution and comprises the following processing steps: taking raw fine powder, soaking the fine powder in aqueous alkali solution overnight, drying the powder and placing the dried powder in a CO2 supercritical liquid extraction device for supercritical liquid extraction; adding 2% aqueous hydrochloric acid solution to extracts to dissolve the extracts, filtering the extracts, adding chloroform to filtrate for extraction, taking subnatant, adjusting pH value to 13, adding the chloroform for extraction, taking the chloroform layer, carrying out separation by alumina column chromatography, taking chloroform-methanol-water subnatant as an eluting agent for elution, collecting eluent, concentrating the eluent, adding acetone-ethanol for recrystallization, washing and drying the crystal, thus obtaining the galanthamine. By adopting the invention to prepare the galanthamine, the product is high in purity and industrialization enlargement is easy to realize.

The invention relates to a method for preparing ginger phenol, which has the characteristics of simple and convenient operation, low pollution and low energy consumption. The method comprises the following process steps of: adding ginger rootstocks into a CO2 supercritical extractor; taking ethyl acetate as an entrainer to obtain an extract; injecting the extract into a molecular still for performing level-3 molecular distillation; and collecting a distillate to obtain the ginger phenol. The method for preparing the ginger phenol has the characteristics of high product purity and easy implementation of industrialization amplification.

The invention relates to a preparation method of the lutein microcapsule with the advantages of simple operation, low costs and high purity. the preparation method comprises the following processing steps of: taking marigold flowers, adding 0.5 to 3 times amount water and 0.005 to 0.1 times amount cellulose, fermenting for 10 to 30 hours under the condition of 30 to 60 DEG C, adding 2 to 30 times amount ethanol, adjusting the PH with NaOH to be alkaline, heating reflux, filtering while hot, adjusting the PH with HCl to be neutral, decompressing and recycling the ethanol, adding the liquid into the macroporous absorption resin column, eluting impurity with water, eluting with 30 to 90% ethanol, collecting eluate, concentrating, adding hexane, methanol, and water with appropriate amount, refrigerating, completely crystallizing, separating the crystallization, washing , drying, grinding, passing 100 mesh sieve, adding modified starch, gelatin and water to make suspension, colloidal milling, and spray drying. The industrialization amplification is easily realized by using the method for preparing the lutein microcapsule.

The invention relates to a preparation method of ursolic acid, which has convenient operation, low pollution and less equipment investment and comprises the following processing steps of: fetching fresh glossy privet leaves; adding water the volume of which is 10 to 30 times of the weight of the glossy privet leaves and soaking for 36 to 96 hours; separating a liquid medicine, filtering, and concentrating; refrigerating for 8 to 24 hours; filtering, collecting filter residues; absorbing with macroporous adsorbent resin columns; eluting with 10 to 50 percent of alcohol; collecting 3 to 8 times column volume of eluent; regulating the pH value to 10 to 11 with stronger ammonia water; filtering; fetching a filtrate; reduced-pressure recovering the alcohol and concentrating; placing overnight; filtering; fetching the filter residues; adding absolute ethyl alcohol to dissolve the filter residues; decoloring active carbon; filtering, concentrating the filtrate; and placing for crystallization. The ursolic acid prepared by the method has high product purity, and the industrial enlargement is easy to realize.