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Preparation method of lutein microcapsule

A technology of lutein and cellulase, which is applied in food preparation, microcapsules, skin care preparations, etc., can solve the problems of cumbersome process, low product purity, and high production cost

Inactive Publication Date: 2009-10-07
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, the extraction of lutein mainly adopts the method of saponification and separation of extraction, the process is cumbersome, the production cost is high, and the product purity is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 10Kg of marigold flowers, add 0.5 times the amount of water and 0.005 times the amount of cellulase, ferment at 60°C for 30 hours, add 2 times the amount of 90% ethanol, adjust the pH to 14 with NaOH, heat and reflux for 4 hours, and keep it hot Filter, adjust the pH to neutral with HCl, recover ethanol under reduced pressure, add the liquid medicine to the X-5 macroporous adsorption resin column, elute with water to remove impurities, elute with 90% ethanol, collect the eluate, concentrate, add n-hexane - Appropriate amount of methanol-water (2:1:0.5), refrigerated to make the crystallization complete, separate the crystallization, wash, dry, pulverize, and pass through a 100 mesh sieve to obtain lutein fine powder, according to lutein:modified starch:gelatin:water =5:3:2:3 ratio to make a suspension, colloid milling, spray drying to obtain 76.3g of lutein microcapsules, the content of which was 31.3% as detected by HPLC.

Embodiment 2

[0021] Take 10Kg of marigold flowers, add 3 times the amount of water and 0.1 times the amount of cellulase, ferment at 30°C for 10 hours, add 30 times the amount of 90% ethanol, adjust the pH to 10 with NaOH, heat and reflux for 4 hours, and keep it hot Filter, adjust the pH to neutral with HCl, recover ethanol under reduced pressure, add the liquid medicine to the H-1020 macroporous adsorption resin column, elute with water to remove impurities, elute with 30% ethanol, collect the eluate, concentrate, add n-hexane - Appropriate amount of methanol-water (2:1:0.5), refrigerated to make the crystallization complete, separate the crystallization, wash, dry, pulverize, and pass through a 100 mesh sieve to obtain lutein fine powder, according to lutein:modified starch:gelatin:water =5:3:2:3 ratio to make a suspension, colloid milling, spray drying to obtain 81.6g of lutein microcapsules, the content of which was 42.5% as detected by HPLC.

Embodiment 3

[0023] Take 10Kg of marigold flowers, add 1 times the amount of water and 0.01 times the amount of cellulase, ferment at 50°C for 20 hours, add 20 times the amount of 90% ethanol, adjust the pH to 13 with NaOH, heat and reflux for 4 hours, and keep it hot Filter, adjust the pH to neutral with HCl, recover ethanol under reduced pressure, add the liquid medicine to the AB-8 macroporous adsorption resin column, elute with water to remove impurities, elute with 70% ethanol, collect the eluate, concentrate, add n-hexane - Appropriate amount of methanol-water (2:1:0.5), refrigerated to make the crystallization complete, separate the crystallization, wash, dry, pulverize, and pass through a 100 mesh sieve to obtain lutein fine powder, according to lutein:modified starch:gelatin:water =5:3:2:3 ratio to make a suspension, colloid mill grinding, spray drying to obtain 79.8g of lutein microcapsules, the content of which was 44.2% as detected by HPLC.

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Abstract

The invention relates to a preparation method of the lutein microcapsule with the advantages of simple operation, low costs and high purity. the preparation method comprises the following processing steps of: taking marigold flowers, adding 0.5 to 3 times amount water and 0.005 to 0.1 times amount cellulose, fermenting for 10 to 30 hours under the condition of 30 to 60 DEG C, adding 2 to 30 times amount ethanol, adjusting the PH with NaOH to be alkaline, heating reflux, filtering while hot, adjusting the PH with HCl to be neutral, decompressing and recycling the ethanol, adding the liquid into the macroporous absorption resin column, eluting impurity with water, eluting with 30 to 90% ethanol, collecting eluate, concentrating, adding hexane, methanol, and water with appropriate amount, refrigerating, completely crystallizing, separating the crystallization, washing , drying, grinding, passing 100 mesh sieve, adding modified starch, gelatin and water to make suspension, colloidal milling, and spray drying. The industrialization amplification is easily realized by using the method for preparing the lutein microcapsule.

Description

technical field [0001] The invention relates to a method for preparing lutein microcapsules, in particular to a method for extracting lutein from marigold flowers and preparing the microcapsules. Background technique [0002] Lutein belongs to carotenoid, widely exists in marigold, corn, cabbage, spinach, lettuce, mung bean, rape and other crops. It is a natural pigment with bright color, strong tinting power and good oxidation resistance , safe and non-toxic, with rich nutritional value. It is widely used in many fields such as food, health products, cosmetics, medicine, tobacco and animal feed. [0003] According to physiological studies, lutein can prevent vision loss and blindness caused by age-related macular degeneration (AMD), and is especially beneficial to the elderly. If lutein can be supplemented in time in food, it can delay the occurrence of eye degenerative diseases. [0004] Lutein is unstable in nature and easily decomposed in the natural state, so it is g...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K31/047A61K47/42A61K8/34A61P27/02A61Q19/00A23K1/16A23L1/29A61K36/28A23K10/12A23K10/30A23K20/105A23K20/189A23K40/00A23L33/00A61K47/36
Inventor 刘东锋范淦彬杨成东
Owner NANJING ZELANG MEDICAL TECH
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