Preparation method of licochalcone A

A technology of licorice chalcone and licorice, which is applied in the field of preparation of licorice chalcone A, to achieve the effects of large-scale production operation, low energy consumption and low pollution

Inactive Publication Date: 2011-04-13
SUZHOU PAITENG BIOLOGICAL MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is no report on the preparation process applicable to the industrialized large-scale production of high-purity licochalcone A

Method used

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  • Preparation method of licochalcone A

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Get licorice (derived from the dry root and rhizome of Glycyrrhiza uralensis Fisch. of the leguminous plant Glycyrrhiza uralensis Fisch.) 10Kg, pulverize, add to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 2%, the extraction pressure is 10MPa, the temperature is 50°C, and the CO 2 The flow rate is 1ml / g crude drug·min, the extraction time is 80min, and the extract is obtained, which is added to the polyamide chromatographic column of nylon 66 and eluted with a mixed solvent of ethanol and water at a ratio of 2:1 as the eluent. The amide film is developed with the above eluent as a supporting test, and the fraction corresponding to Rf=5-5.5 on the polyamide film is collected, the solvent is recovered, acetone is added to crystallize, washed and dried to obtain yellow needle crystals - licochalcone A66.5g, detected by HPLC, the purity is 92.5%, UV, IR, MS, 2 HNMR, 13 ...

Embodiment 2

[0026] Get licorice (derived from the dry root and rhizome of Glycyrrhiza inflata Bat. of the leguminous plant Glycyrrhiza inflata Bat.) 10Kg, pulverize, join in CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 4%, the extraction pressure is 30MPa, the temperature is 70°C, and the CO 2 The flow rate is 3ml / g crude drug·min, the extraction time is 120min, and the extract is obtained, which is added to the chromatographic column of nylon 6 polyamide and eluted with ethanol-water mixed solvent with a ratio of 2:1 as eluent. The polyamide film is developed with the above eluent as a supporting test, collect the fraction corresponding to Rf=5-5.5 on the polyamide film, recover the solvent, add acetone to crystallize, wash and dry to obtain yellow needle crystals-licorice chal Ketone A56.2g, detected by HPLC, the purity is 94.8%, UV, IR, MS, 2 HNMR, 13 The data characterizing its phy...

Embodiment 3

[0028] Get 10Kg of licorice (derived from the dry root and rhizome of the leguminous plant Glycyrrhiza glabra L.), pulverize it, and add it to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 3%, the extraction pressure is 20MPa, the temperature is 65°C, and the CO 2 The flow rate is 2ml / g crude drug·min, the extraction time is 100min, and the extract is obtained, which is added to the chromatographic column of nylon 66 polyamide, eluted with ethanol-water mixed solvent with a ratio of 2:1 as the eluent, and the same kind of The polyamide film is developed with the above eluent as a supporting test, collect the fraction corresponding to Rf=5-5.5 on the polyamide film, recover the solvent, add acetone to crystallize, wash and dry to obtain yellow needle crystals-licorice chal Ketone A73.2g, detected by HPLC, the purity is 95.9%, UV, IR, MS, 2 HNMR, 13 The data characterizing its...

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Abstract

The invention relates to a preparation method of licochalcone A, which is convenient to operate, less in pollution and low in energy consumption. The method comprises the following steps: smashing liquorice; adding the smashed liquorice into a CO2 supercritical extractor; adding ethanol as entrainer; and carrying out extracting to obtain extract; adding the extract to a polyamide chromatographic column; adding ethanol-water mixed solvent as eluant for eluting, wherein the ratio of ethanol to water is 2:1; collecting eluent; and recovering the solvent, adding acetone for crystallization, washing and drying, thus obtaining the licochalcone A. The licochalcone A prepared by the method has high purity and is easy to realize industry magnification.

Description

technical field [0001] The invention relates to a preparation method of licochalcone A, in particular to a preparation method of extracting licochalcone A from plants. Background technique [0002] Licochalcone A (Licochalcone A), molecular formula: C 21 h 22 o 4 , Molecular weight: 338.402, CAS registration number: 58749-227, mainly found in leguminous licorice, licorice glabra, and licorice inflating fruit. Its molecular formula is as follows: [0003] [0004] Modern studies have shown that licochalcone A has anti-inflammatory, anti-tumor, anti-microbial, anti-HIV, anti-allergic and other effects, and it is also used as a raw material for the synthesis of other active derivatives. [0005] The medicinal licorice is the dry root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of the leguminous plant Glycyrrhiza uralensis Fisch. It has the effects of invigorating the spleen and replenishing qi, clearing heat and detoxif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/84C07C45/78
CPCY02P20/54
Inventor 王峰王琳张发成
Owner SUZHOU PAITENG BIOLOGICAL MEDICAL TECH
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