Preparation method of ursolic acid
A technology of ursolic acid and filtration, applied in the field of preparation of ursolic acid, can solve the problems of serious pollution, unfavorable large-scale production operations, low ursolic acid content and the like
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Embodiment 1
[0018] Take 10Kg of fresh privet leaves, add 10 times its weight in water and soak for 36 hours, separate the medicinal liquid, filter, concentrate, refrigerate for 8 hours, filter, collect the filter residue, and absorb it through an AB-8 macroporous adsorption resin column. Elute with 10% ethanol, collect 3 times column volume eluate, adjust pH 10-11 with concentrated ammonia water, filter, take filtrate, recover ethanol under reduced pressure and concentrate, leave overnight, filter, take filter residue, add absolute ethanol Dissolve, decolorize with active carbon, filter, concentrate the filtrate, place to crystallize, separate the crystals, wash and dry to obtain 25.8g of ursolic acid, detected by HPLC, with a purity of 98.1%.
Embodiment 2
[0020] Take 10Kg of fresh privet leaves, add 30 times its weight in water and soak for 96 hours, separate the medicinal liquid, filter, concentrate, refrigerate for 24 hours, filter, collect the filter residue, and adsorb through the H-103 macroporous adsorption resin column. Elute with 50% ethanol, collect 8 times the column volume eluate, adjust the pH to 10-11 with concentrated ammonia water, filter, take the filtrate, recover the ethanol under reduced pressure and concentrate, leave it overnight, filter, take the filter residue, add absolute ethanol Dissolve, decolorize with activated carbon, filter, concentrate the filtrate, place to crystallize, separate the crystals, wash and dry to obtain 33.8g of ursolic acid, detected by HPLC, with a purity of 98.1%.
Embodiment 3
[0022] Take 10Kg of fresh privet leaves, add 20 times its weight in water and soak for 72 hours, separate the liquid medicine, filter, concentrate, refrigerate for 12 hours, filter, collect the filter residue, absorb it through a D101 macroporous adsorption resin column, and use 30 Elute with % ethanol, collect 5 times the column volume eluate, adjust the pH to 10-11 with concentrated ammonia water, filter, take the filtrate, recover the ethanol under reduced pressure and concentrate, leave it overnight, filter, take the filter residue, add absolute ethanol to dissolve, Activated carbon was decolorized, filtered, and the filtrate was concentrated, placed to crystallize, separated and crystallized, washed and dried to obtain 32.4 g of ursolic acid, which was detected by HPLC with a purity of 99.2%.
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