Preparation method of ursolic acid

A technology of ursolic acid and filtration, applied in the field of preparation of ursolic acid, can solve the problems of serious pollution, unfavorable large-scale production operations, low ursolic acid content and the like

Inactive Publication Date: 2010-06-30
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In the prior art, the extraction and separation method of ursolic acid mainly adopts the organic solvent method, etc., but the content of ursolic ac...

Method used

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  • Preparation method of ursolic acid

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Effect test

Embodiment 1

[0018] Take 10Kg of fresh privet leaves, add 10 times its weight in water and soak for 36 hours, separate the medicinal liquid, filter, concentrate, refrigerate for 8 hours, filter, collect the filter residue, and absorb it through an AB-8 macroporous adsorption resin column. Elute with 10% ethanol, collect 3 times column volume eluate, adjust pH 10-11 with concentrated ammonia water, filter, take filtrate, recover ethanol under reduced pressure and concentrate, leave overnight, filter, take filter residue, add absolute ethanol Dissolve, decolorize with active carbon, filter, concentrate the filtrate, place to crystallize, separate the crystals, wash and dry to obtain 25.8g of ursolic acid, detected by HPLC, with a purity of 98.1%.

Embodiment 2

[0020] Take 10Kg of fresh privet leaves, add 30 times its weight in water and soak for 96 hours, separate the medicinal liquid, filter, concentrate, refrigerate for 24 hours, filter, collect the filter residue, and adsorb through the H-103 macroporous adsorption resin column. Elute with 50% ethanol, collect 8 times the column volume eluate, adjust the pH to 10-11 with concentrated ammonia water, filter, take the filtrate, recover the ethanol under reduced pressure and concentrate, leave it overnight, filter, take the filter residue, add absolute ethanol Dissolve, decolorize with activated carbon, filter, concentrate the filtrate, place to crystallize, separate the crystals, wash and dry to obtain 33.8g of ursolic acid, detected by HPLC, with a purity of 98.1%.

Embodiment 3

[0022] Take 10Kg of fresh privet leaves, add 20 times its weight in water and soak for 72 hours, separate the liquid medicine, filter, concentrate, refrigerate for 12 hours, filter, collect the filter residue, absorb it through a D101 macroporous adsorption resin column, and use 30 Elute with % ethanol, collect 5 times the column volume eluate, adjust the pH to 10-11 with concentrated ammonia water, filter, take the filtrate, recover the ethanol under reduced pressure and concentrate, leave it overnight, filter, take the filter residue, add absolute ethanol to dissolve, Activated carbon was decolorized, filtered, and the filtrate was concentrated, placed to crystallize, separated and crystallized, washed and dried to obtain 32.4 g of ursolic acid, which was detected by HPLC with a purity of 99.2%.

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Abstract

The invention relates to a preparation method of ursolic acid, which has convenient operation, low pollution and less equipment investment and comprises the following processing steps of: fetching fresh glossy privet leaves; adding water the volume of which is 10 to 30 times of the weight of the glossy privet leaves and soaking for 36 to 96 hours; separating a liquid medicine, filtering, and concentrating; refrigerating for 8 to 24 hours; filtering, collecting filter residues; absorbing with macroporous adsorbent resin columns; eluting with 10 to 50 percent of alcohol; collecting 3 to 8 times column volume of eluent; regulating the pH value to 10 to 11 with stronger ammonia water; filtering; fetching a filtrate; reduced-pressure recovering the alcohol and concentrating; placing overnight; filtering; fetching the filter residues; adding absolute ethyl alcohol to dissolve the filter residues; decoloring active carbon; filtering, concentrating the filtrate; and placing for crystallization. The ursolic acid prepared by the method has high product purity, and the industrial enlargement is easy to realize.

Description

technical field [0001] The invention relates to a method for preparing ursolic acid, in particular to a method for extracting ursolic acid from plants. Background technique [0002] Ursolic acid, synonym: ursolic acid, ursolic acid, molecular formula: C 30 h 48 o 3 , molecular weight: 456.707, CAS accession number: 77-52-1, the structural formula is as follows: [0003] [0004] Ursolic acid is a natural compound with various physiological activities such as antitumor, antibacterial, antiviral, and diuretic, and widely exists in various plants. [0005] Ligustrum lucidum is the leaf of Ligustrum lucidum Ait., a plant of Oleaceae. It has the functions of expelling wind, improving eyesight, reducing swelling, and relieving pain. It contains a large amount of ursolic acid. [0006] In the prior art, the extraction and separation methods of ursolic acid mainly use organic solvent methods, etc., but the content of ursolic acid obtained by these extraction processes is low,...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 刘东锋范淦彬杨成东
Owner NANJING ZELANG MEDICAL TECH
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