Preparation method of polyporus polysaccharide

Polyporus polysaccharide and Polyporus polysaccharide technology are applied in the field of preparation of Polyporus polysaccharide, can solve the problems of serious pollution, low polysaccharide content, unfavorable large-scale production operation, etc., and achieve the effects of less pollution, simple operation and favorable large-scale production operation.

Inactive Publication Date: 2009-12-16
SUZHOU PAITENG BIOLOGICAL MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the extraction and separation method of Polyporus polysaccharide mainly adopts water extraction and organic solvent treatment, but the content of Polyporus polysaccharide obtained by these extraction processes is low, the pollution is serious, and it is not conducive to large-scale production operations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 10Kg of Polyporus coarse powder, add 4 times the amount of water to decoct once, 2 hours each time, filter, combine the filtrate, concentrate, add ethanol to sink to alcohol content of 50%, let it stand for 24 hours, dissolve the precipitate with water, Filtrate, concentrate the filtrate, add it to the H-1020 macroporous adsorption resin column, elute with water, collect the eluate with 2 times the column volume, concentrate, dry at low temperature, and pulverize to obtain 561g of Polyporus polysaccharide, which is detected by spectrophotometry , with a purity of 95.6%.

Embodiment 2

[0017] Take 10Kg of the coarse powder of Polyporus sinensis, add 10 times the amount of water to decoct 3 times, 2 hours each time, filter, combine the filtrate, concentrate, add ethanol to sink to alcohol content of 70%, let it stand for 24 hours, and dissolve the precipitate with water. Filtrate, concentrate the filtrate, add it to an X-5 macroporous adsorption resin column, elute with water, collect 5 times the column volume eluate, concentrate, dry at low temperature, and pulverize to obtain 998 g of Polyporus polysaccharide, which is detected by spectrophotometry , with a purity of 92.1%.

Embodiment 3

[0019] Take 10Kg of Polyporus sativa powder, add 8 times the amount of water to decoct twice, 2 hours each time, filter, combine the filtrate, concentrate, add ethanol to sink to alcohol content of 60%, let it stand for 24 hours, and dissolve the precipitate with water. Filtrate, concentrate the filtrate, add to the AB-8 macroporous adsorption resin column, elute with water, collect 4 times the column volume eluate, concentrate, dry at low temperature, and pulverize to obtain 861g of Polyporus polysaccharide, which is detected by spectrophotometry , with a purity of 98.4%.

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PUM

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Abstract

The invention relates to a preparation method of polyporus polysaccharide, which is characterized by simple operation, little pollution and low equipment investment and comprises the following processing steps: taking coarse polyporus powder, adding 4-10 times amount of water for decoction for 1-3 times, with 2h once, filtering decoction liquid, combining filtrate, concentrating the filtrate, adding ethanol for alcohol precipitation until the alcohol content is 50-75%, standing for 24h, dissolving precipitate by water, filtering dissolved matters, concentrating filtrate, adding concentrate to a macroporous resin absorption column, using water for elution, collecting eluent in 2-5 times column volume, concentrating the eluent, drying the elution at low temperature and grinding the dried matters, thus obtaining the polyporus polysaccharide. By adopting the invention to prepare the polyporus polysaccharide, the product is high in purity and industrialization enlargement is easy to realize.

Description

technical field [0001] The invention relates to a preparation method of polyporus polysaccharides, in particular to a method for extracting polyporus polysaccharides from plants. Background technique [0002] Polyporus polysaccharide is an active ingredient contained in the traditional Chinese medicine polyporus. Polyporus is a medicinal fungus, which is listed as a treasure in "Shenlong Materia Medica". Polyporus can cure inflammation, diuresis, detoxify, protect liver and liver, and has auxiliary effects on patients with fatty liver. Recent studies have shown that Polyporus polysaccharide is a good immune regulator, which can inhibit the growth of cancer cells and improve the spirit and appetite of tumor patients. Polyporus polysaccharide can also significantly improve the symptoms of hepatitis patients, repair liver tissue damage, and consolidate the curative effect. And it can effectively turn yang into yin. The human body absorbs polyporus polysaccharides well, and l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 王琳范淦彬
Owner SUZHOU PAITENG BIOLOGICAL MEDICAL TECH
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