Rapid propagation method of astragalus membranaceus calluses and astragalus membranaceus suspension cells

A technology of suspension cells and callus, applied in biochemical equipment and methods, plant cells, tissue culture, etc., can solve the problems of yield and quality decline, lack of natural resources of Astragalus membranaceus, etc., achieve less pollution, facilitate large-scale production operations, fast growth effect

Inactive Publication Date: 2013-12-18
NANJING ZELANG AGRI DEV
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the dosage of Astragalus membranaceus is increasing, but the natural resources of Astragalus membranaceus are poor. During the cultivation process, due to the influence of diseases and insect pests, the yie

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid propagation method of astragalus membranaceus calluses and astragalus membranaceus suspension cells

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1

[0018] Take the young leaves of outdoor astragalus in spring, soak the buds, washing powder for 4 minutes, rinse with running water for 50 minutes, sterilize with sodium hypochlorite for 12 minutes, rinse with sterile water 5 times, and inoculate it into MS medium for callus induction. Additional hormone 1mg / LNAA, 3mg / L2,4-D, 30g / L sucrose, 7g / L agar, pH=8, temperature 25℃, humidity 50%-70%, light intensity 2000LX; light period 14h dark period 10h ; Take the induced tender green loose callus and inoculate it into a 100 mL Erlenmeyer flask containing 40 mL MS liquid medium. The medium formula is MS+0.5mg / LNAA+1mg / LIBA. After inoculation, place it on a rotary shaker Shake culture, the rotation speed of the shaker is 100-120 r / min, the temperature is 25℃, the initial liquid culture callus grows vigorously, after shaking the culture medium, let it stand for a while, and subculture with a suspension cell mass of moderate particles. 200 mL Erlenmeyer flask was fille...

Example Embodiment

[0019] Example 2

[0020] Take the young leaves of outdoor astragalus in spring, soak the buds, washing powder for 4 minutes, rinse with running water for 50 minutes, sterilize with sodium hypochlorite for 12 minutes, rinse with sterile water 5 times, and inoculate it into MS medium for callus induction. Additional hormone 0.1mg / LNAA, 3mg / L2,4-D, sucrose 30g / L, agar 7g / L, pH=8, temperature 25℃, humidity 50%-70%, light intensity 2000LX; photoperiod 14 h dark Period 10h; take the induced tender green loose callus and inoculate it into a 100 mL Erlenmeyer flask containing 40 mL MS liquid medium. The medium formula is MS+0.5mg / LNAA+2mg / LIBA. After inoculation, place it in a rotary Shaker and shake culture, the speed of the shaker is 100-120 r / min, the temperature is 25 ℃, the initial liquid culture callus grows vigorously, after shaking the culture medium, let it stand for a while, and subculture with the suspension cell mass of moderate particles. Change to 200 mL Erlenmeyer flask ...

Example Embodiment

[0021] Example 3

[0022] Take the young leaves of outdoor astragalus in spring, soak the buds, washing powder for 4 minutes, rinse with running water for 50 minutes, sterilize with sodium hypochlorite for 12 minutes, rinse with sterile water 5 times, and inoculate it into MS medium for callus induction. Additional hormone 1mg / LNAA, 3mg / L2,4-D, 30g / L sucrose, 7g / L agar, pH=8, temperature 25℃, humidity 50%-70%, light intensity 2000LX; light period 14h dark period 10h ; Take the induced tender green loose callus and inoculate it into a 100 mL Erlenmeyer flask containing 40 mL MS liquid medium. The medium formula is MS+1mg / LNAA+1mg / LIBA. After inoculation, place it on a rotary shaker and shake Cultivate with a shaker at a speed of 100-120 r / min and a temperature of 25°C. The callus grows vigorously in the initial liquid culture. After shaking the culture medium, let it stand for a while, and use a suspension cell mass with moderate particles for subculture, replace with 200 Incubat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a rapid propagation method of astragalus membranaceus calluses and astragalus membranaceus suspension cells. The rapid propagation method comprises the steps of obtaining a sterile explant, inducing calluses, culturing the suspension cells, regulating and controlling the activity of an enzyme in the suspension cells by using rare earth and the content of flavone saponins, and the like. The astragalus membranaceus suspension cells prepared by the method provided by the invention have high content of astragalus membranaceus saponins. The method provided by the invention realizes goal-directed regulation and control on the astragalus membranaceus suspension cells, thus a large amount of pharmaceutical raw materials can be obtained in short term and the production cost is effectively reduced.

Description

technical field [0001] The invention relates to the cultivation of astragalus suspension cells under tissue culture conditions, in particular to the regulation of astragalus saponins in the suspension cells by rare earth cerium nitrate. Background technique [0002] Astragalus, also known as Mianqi, Mianhuangqi, is a leguminous plant, Astragalus membranaceus. It is a national third-level protected plant and is an endangered species. It is mainly distributed in northern China. It is a relatively rare Chinese medicinal material. Its roots are used as medicine. With a long history, it has the effects of invigorating qi and solidifying the exterior, diuresis and detoxification, astringing sores and promoting granulation, and nourishing qi and invigorating the middle. At present, the dosage of Astragalus membranaceus is increasing, but the natural resources of Astragalus membranaceus are poor. During the cultivation process, due to the influence of diseases and insect pests, the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/04C12N5/02
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap