Method for preparing indirubin

Abstract

The invention relates to a method for preparing indirubin with simple and convenient operation, small pollution and little equipment investment. The method comprises the following technological steps:adding methanol with the quantity of 5-10 times of raw materials into the raw materials, extracting by ultrasonic for 1-3 times, extracting for 0.2-1 hour each time, merging extracting liquid, recovering the methanol by depressurization, concentrating, filtering liquor by a macroporous resin column, eluting with water firstly, removing impurities, then eluting with ethyl acetate, concentrating eluent, adopting chromatographic separation by an alumina column, eluting with chloroform-ethyl acetate, concentrating eluent, recrystallizing with methanol-acetone, washing, drying to obtain the indirubin. By adopting the invention to prepare the indirubin, the product purity is high and industrialization amplification is easy to realize.

Description

technical field

[0001] The invention relates to a method for preparing indirubin, in particular to a method for extracting indirubin from plants. Background technique

[0002] Indirubin, whose chemical name is 2,2'-biindole-3,3'-dione, is a drug discovered in my country with a chemical structure different from known antineoplastic drugs, mainly found in Daqing Leaves, Banlangen, Qingdai and other Chinese herbal medicines. Its molecular formula is C 15 h 10 N 2 o 2 , the formula weight is 262.26. Indirubin is weakly alkaline, insoluble in water, hardly soluble in cold ethanol, but soluble in ethyl acetate, etc. and is volatile. Indirubin has a good effect on the treatment of chronic myelogenous leukemia.

[0003] In the prior art, the extraction and separation methods of indirubin mainly include chloroform extraction, ethanol extraction and ethyl acetate extraction, etc., but the content of indirubin obtained by these extraction processes is very low, and it is not cond...