The invention discloses a fluorescent probe for monitoring
lipid peroxidation processes in different subcellular organelles, and belongs to the field of
fine chemical engineering. The fluorescent probe is prepared through the steps of: enabling
fluorine boron pyrrole derivatives to react with
cinnamic aldehyde, wherein
piperidine is used as a catalyst, and a 4 angstrom
molecular sieve is used as a dehydrating agent; and performing quaternization on the obtained reactant so as to obtain molecules of the fluorescent probe. The probe contains active groups such as
triphenylphosphine cations,
morpholine and
quaternary ammonium salt cations, the probe has a single-minded
subcellular organelle targeting property because of the different active groups, and the probe can be optionally positioned on subcellular organelles such as mitochondria, lysosomes and
cell membranes. The probe has zero
toxin and zero side effects and is suitable for monitoring the
lipid peroxidation processes in the subcellular organelles in different physiological processes through a fluorescent
microscope or flow type
cell analyzing technology. The probe is stable in
solid condition nature and easy to store for a long time, an intuitional succinct detecting
reagent with low cost is provided for inquiring processes relevant to the
lipid peroxidation in cells, and microcosmic structural information is provided for detection by a
subcellular organelle targeting property function.