The invention relates to an environment-friendly coating, in particular to a biological-enzyme inner-wall environment-friendly coating which can be used for decorating the surfaces of indoor wall surfaces, ceilings, gypsum boards, and the like and can clear indoor formaldehyde, release anions and purify indoor air by biological enzymes. The preparation technology of the biological-enzyme inner-wall environment-friendly coating comprises the following steps: firstly, sequentially adding a dispersant, a wetting agent, a foam suppressor, a thickening agent and a mildewproof algaecide at a low speed; sequentially adding titanium pigment, a pH regulator, coarse whiting, calcined kaolin, talcum powder and anion fungi at a medium speed; and sequentially adding latex, a film-forming additive, an anti-freezing agent, a preservative, a foam suppressor, a flatting agent, 40 percent of biological-enzyme solution and H2O at a low speed and filtering and packaging after detecting to be qualified. The biological-enzyme inner-wall environment-friendly coating has the advantages of clearing the indoor formaldehyde, releasing the anions and purifying the air.