The invention relates to application of chelerythrine to the treatment of poultry drug-resisting colibacillosis and in particular relates to a medicine which is prepared from the chelerythrine and other ministerial drugs and adjuvant drugs and is used for treating the poultry drug-resisting colibacillosis. The medicine provided by the invention can be prepared into suitable traditional Chinese medicine dosage forms including tablets, capsules, granules, pills, powder, ointment, sublimed preparations, powdered preparations, solutions, injections and the like according to market requirements and production conditions. The medicine provided by the invention has the characteristics of low medicine cost, simple production process, controllable quality and the like. The effective rate on the poultry drug-resisting colibacillosis reaches 93.5 percent, the death and culling rate can be reduced, the uniformity of chicken flocks is improved and the feed reward is increased; meanwhile, the medicine is environmentally friendly and has no pollution.