43 results about "Methyltransferase Gene" patented technology

The invention discloses a kit for genetic detection of breast cancer. The kit comprises a specific primer pair and a specific probe pair which are used for detecting the mononucleotide polymorphism loci genotype of an estrogen receptor alpha gene (ER-alpha), a catechol-o-methyltransferas gene (COMT), a glutathione-s-transferase P1 gene (GSTP1), a human breast cancer susceptibility gene 1 (BRCA1), and a human breast cancer susceptibility gene 2(BRCA2), a fluorescent quantitative PCR general component and the like. The kit can evaluate the genetic risk of individuals suffered from the breast cancer by detecting the polymorphism loci genotype of the genes closely related to the genetic risk of the breast cancer at the same time.

The invention provides an o-methyltransferase with a wide range of substrates, and can be applied to oxygen methyl transfer in a biosynthesis process of an organic compound. An experiment proves the catalytic activity of the o-methyltransferase, and shows that the o-methyltransferase can be applied to a plurality of substrates. The invention further provides a synthesis method for a hydroquinone lactone compound. The hydroquinone lactone compound is obtained by transforming an o-methyltransferase gene and a hydroquinone lactone synthetic gene into yeast cells, carrying out fermentation cultivation and extracting a product.

The present invention discloses genetically engineered bacteria and application thereof in production of coenzyme Q10. The genetically engineered bacteria comprises coenzyme Q10 producing bacteria and desired genes transferred into the coenzyme Q10 producing bacteria, and the desired genes are a demethylnaphthoquinone methyltransferase gene, a 2-octo isoprene-3-methyl-6-methoxy-1,4-hydroquinone hydroxylase gene and a 3-demethyl ubiquinone-93-methyl transferase gene. Rate-limiting enzyme genes UbiE, UbiG and UbiF in a coenzyme Q10 synthetic route are transferred into rhodobacter sphaeroides, and by in-series over-expression of the three rate-limiting enzyme genes UbiE, UbiG and UbiF, coenzyme Q10 synthesizing capability of the rate-limiting enzyme genes can be strengthened; and compared with the rhodobacter sphaeroides with no desired gene being transformed, by use of the genetically engineered bacteria for coenzyme Q10 producing, the yield of the coenzyme Q10 is increased by more than 20%.

The invention belongs to the technical field of the biology, and relates to a method for regulating the growth of plants and an application thereof, in particular to an application of paddy rice Histone-lysine methyltransferase gene which is associated to the Epigenetics in the regulation of the plant growth. The name of the gene is SDG714, and the accession number is NM_001051801. Through a designed primer, a target gene entering plant expression carrier is obtained, and the plant Arabidopsis is transformed in an Agrobacterium rhizogenes medium transformation mode. Compared with a contrast group, in the plant of the over-expression SDG714, the methylation level of the histone H3K9 is obviously improved to cause the decrease of a great amount of genes which are related to the growth of the plant and to seriously inhibit the growth of the Arabidopsis; and once the SDG714 which is over-expressed is removed in the plant with the growth being inhibited, the plant can continue to grow. The method for regulating the growth of the plant has high real application value and promising application prospect.

The invention provides a rose characterized by comprising a flavone and malvidin added by a genetic modification method. The flavone and malvidin are typically produced by expression of a transferred flavone synthase gene, pansy flavonoid 3′,5′-hydroxylase gene and anthocyanin methyltransferase gene. The flavone synthase gene is, for example a flavone synthase gene of the family Scrophulariaceae, and specifically it may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae. The flavonoid 3′,5′-hydroxylase gene is, for example, the pansy flavonoid 3′,5′-hydroxylase gene. The anthocyanin methyltransferase gene is, for example, the methyltransferase gene of torenia of the family Scrophulariaceae.

De novo DNA cytosine methyltransferase polynucleotides and polypeptides and methods for producing said polypeptides are disclosed. Also disclosed are methods for utilizing de novo DNA cytosine methyltransferase polynucleotides and polypeptides in diagnostic assays, in vitro DNA methylation assays for screening agonists and antagonists, and therapeutic applications such as the treatment of neoplastic disorders.

The invention discloses a genetic engineering strain capable of accumulating emodin. An original strain of the genetic engineering strain is aspergillus terreus capable of producing emodin or downstream derivatives thereof, the genetic engineering strain is prepared by mutating O-methyltransferase genes (gedA) in the aspergillus terreus. Compared with the original strain, the genetic engineering strain can be used for accumulating emodin.

The invention discloses a transmethylase gene participating in synthesis of sanguinarine and chelerythrine in macleaya cordata and application of the transmethylase gene. Six transmethylase genes participating in synthesis of sanguinarine and chelerythrine are found in a macleaya genome for the first time and comprise the Mc2833 gene, the Mc769 gene, the Mc8567 gene, the Mc833 gene, the Mc830 gene and the Mc9487 gene. A saccharomyces cerevisiae system is utilized for verifying, several steps of reactions are verified through upstream precursor feeding, and synthesis of sanguinarine and chelerythrine intermediates can be achieved. The invention further discloses a molecular mechanism of sanguinarine in synthesis of macleaya cordata, and theoretical bases and molecular assisted breeding objectives are provided for sanguinarine and chelerythrine content macleaya cordata breeding; meanwhile, valuable experience is provided for in-vitro artificial synthesis of sanguinarine and chelerythrine.

The invention discloses a methyltransferase gene in a betaine synthetic pathway and application thereof. A method for preparing the methyltransferase gene comprises the following steps of: cloning a glycylsarcosine N-methyltransferase gene (ApGSMT2) and a dimethylglycine N-methyltransferase gene (ApDMT2) from aphanothece halophytica; substituting a codon which is in the ApGSMT2 and the ApDMT2 andis relatively low in appearance chance in a higher plant by a codon which is favorable by the higher plant so as to generate the ApGSMT2d gene and the ApDMT2d gene; and constructing series genes of which the encoding products are respectively positioned in chondriosomes by the method for fusing a protein-positioned guide peptide sequence and the methyltransferase gene. The ApGSMT2 uses glycine and creatine as substrates to catalyze glycine methylation, the ApDMT catalyzes the methylation of dimethylglycine, and the ApGSMT2 and the ApDMT coordinate to catalyze the synthesis of the glycine betaine. The ApGSMT2/ApDMT2 or modifying genes thereof are transplanted into crops, such as corns, wheat, cotton, bean, and tobacco in a synergistic way, and high concentration of glycine betaine is accumulatd ein cells, so that the stress resistance of plants is obviously improved.

The invention discloses COMT3 in Morus notabili and application thereof. The COMT is prepared by coding 374 amino acids with a COMT3 gene having a full length of 1125 bp and a PI value of 4.98. The Morus notabili COMT3 gene is cloned, then a prokaryotic expression system is constructed, enzyme activity determination is carried out after recombinant expression, the activity of the COMT is measuredto be high, and therefore, the Morus notabili COMT gene can be used as a target gene of engineering bacteria, and the Morus notabili COMT3 can also be used for catalyzing conversion of 5-hydroxytryptamine into 5-methoxy-tryptamine or conversion of N-acetyl-5-hydroxytryptamine into melatonin in vitro and has good application prospects.

The invention belongs to the field of plant breeding, relates to the field of plant biotechnology breeding, and particularly relates to a method for creating a phenotypic variation transgenic plant byutilizing a methyltransferase gene. According to the method, an agrobacterium-mediated method is used for introducing a chemical induction promoter and the arabidopsis DNA methyltransferase gene AtMET1 into an 84K poplar genome to obtain an AtMET1 84K poplar plant, a sterile in-vitro leaf AtMET1 of the transgenic plant is induced to express for 3 hours, 12 hours and 24 hours by using estrogen, and then the leaf is induced to regenerate to obtain the phenotypic variant plant. According to the research, the chemical induction promoter is used for artificially promoting exogenous gene expression, phenotypic variation germplasm is created, and plant breeding resources are enriched.

The invention belongs to the technical field of bioengineering, and discloses a gene-engineering bacterium, a construction method of same, and a method of producing vanillin. In the gene-engineering bacterium, the metabolic pathway of 3-dehydro-shikimic acid is modified, wherein a shikimic acid dehydrogenase encoding gene is deleted and a vanillin degradation related gene is also deleted, so that the bacterial strain can accumulate a large amount of 3-dehydro-shikimic acid; in addition, expression on a 3-dehydro-shikimic acid dehydratase gene, an O-transmethylase gene and a vanillic acid reductase gene and two genes in the metabolic pathway of the 3-dehydro-shikimic acid are enhanced, so that yield of the vanillin is increased, degradation of the vanillin is reduced and quantity of the vanillin in a fermentation liquid is increased, thus improving the capability of fermenting production of the vanillin by the bacterial strain. A test proves that the gene-engineering bacterium uses glucose or glycerol as a substrate for producing the vanillin, wherein the yield of the vanillin is increased significantly. A vanillin fermentation liquid produced with the method is free of an isovanillin impurity, so that subsequent separation is easy to carry out.

The invention discloses a transmethylase gene in a betaine synthesis process, and modification and application thereof. The method comprises the following steps: cloning glycine sarcosine N-transmethylase gene (ApGSMT2) and dimethyl glycine N-transmethylase gene (ApDMT2) from aphanothece halophytica, substituting codons with lower occurrence possibility in higher plants in the ApGSMT2 and the ApDMT2 with higher plant preferred codons to generate a gene ApGSMT2d and a gene ApDMT2d, and constructing the serial genes, of which the coded products are respectively positioned in the chloroplast, byprotein locating targeting sequence and transmethylase gene fusion. Glycine and sarcosine are used as the substrates, the methylation of the glycine is catalyzed, the methylation of the dimethyl glycine is catalyzed by ApDMT, and the glycinebetaine is synthesized under the concerted catalysis. The ApGSMT2/ApDMT2 or modifier genes thereof are synergically transferred into corn, wheat, cotton, soybean, tobacco and other crops, so high-concentration glycinebetaine is accumulated in the cells, thereby obviously enhancing the stress tolerance of the plant.

The invention discloses ASMT12 in Morus notabili and application thereof. The ASMT12 in Morus notabili is obtained by coding 370 amino acids with an ASMT12 gene having a full length of 1110 bp and a PI value of 6.57. The Morus notabili ASMT12 gene is cloned, then a prokaryotic expression system is constructed, enzyme activity determination is carried out after recombinant expression, the activityof the N-acetyltryptamine O-methyltransferase is measured to be high, and therefore, the Morus notabili ASMT12 gene can be used as a target gene of engineering bacteria, can also be used for catalyzing conversion of N-acetyl-5-hydroxytryptamine into melatonin or catalyzing conversion of 5-hydroxytryptamine into 5-methoxy-tryptamine in vitro, and has good application prospects.