9 results about "Juvenile hormone" patented technology

The invention discloses application of expression of a targeted silence TdSb-sbd gene in increasing the egg laying amount of female trichogramma dendrolimus, and belongs to the technical field of biological prevention and control. The invention discovers that the SPS protein (coding gene TdSb-sbd) of trichogramma dendrolimus can interact with the juvenile hormone degradation key enzyme JHEH1 protein. After the TdSb-sbd gene is silent in a targeted manner, the expression level of the gene can be reduced, and the expression of an interaction protein coding gene Tdjheh1 of the gene is reduced, so that the degradation pathway of juvenile hormone (JH) is inhibited, and the titer of juvenile hormone in trichogramma is improved. The high juvenile hormone level can promote ovarian development and ovum generation of trichogramma, and finally the egg laying amount of female bees is remarkably increased. The trichogramma reproductive capacity is directly regulated and controlled through a molecular biology means, and a new technical scheme is provided for improving the trichogramma dendrolimus reproductive capacity and the field pest control efficiency on a large scale.

The present application belongs to the field of biotechnology, and particularly relates to a method for inducing expression of a target protein gene based on juvenile hormone and application thereof. The present application provides a method for inducing expression of a target protein gene based on juvenile hormone on the basis of long-term research on juvenile hormone regulating expression of an insect gene. The method induces protein expression by juvenile hormone, realizes controllable switch of protein expression, and reduces side effects of protein expression on organisms. The method is harmless to human and mammals, safe to other organisms and environment, and can be used for disease treatment, immunity enhancement, and new function of organisms. The method has good practical application value in disease treatment, disease resistance of animals and plants, and new function of organisms.

The invention discloses miR-10490-5p for preventing and treating spodoptera frugiperda and application thereof, miR-10490-5p targeting a key gene for growth and development of the spodoptera frugiperda is screened for the first time, and after an analogue agomiR-10490-5p of the miR-10490-5p is injected, it is found that the miR-10490-5p can inhibit normal expression of a juvenile hormone epoxy hydrolase (JHEH) gene of the spodoptera frugiperda, and the miR-10490-5p can inhibit the growth and development of the spodoptera frugiperda. Therefore, the growth and development of the spodoptera frugiperda larvae are slowed down, the feed intake is reduced, and the death rate is increased. In addition, by adding the analogue agomiR-10490-5p into the feed, the growth of the spodoptera frugiperda can be inhibited, the food intake is reduced, and the death rate is increased. The invention provides a new target and technical support for establishing a new strategy for preventing and controlling the spodoptera frugiperda based on the miRNA.

The application relates to application of juvenile hormone in relieving thiamethoxam poisoning risk of Apis mellifera ligustica, and the juvenile hormone is used for accelerating the metabolism rate of thiamethoxam in the body of the honeybee; the juvenile hormone is JH III. The application method is as follows: JH III is first dissolved with methanol to prepare a JH III mother liquor with a concentration of 5 ng / muL, is blown dry with nitrogen, is re-dissolved with an equal volume of ultrapure water, is then added into sugar water feed to prepare a mixed solution with a JH III concentration of 1 ng / muL, and is fed to the honeybee. The application can significantly accelerate the metabolism of thiamethoxam by regulating the hormone level in the body of the honeybee, and can further enhance the detoxification capacity, so as to improve the survival rate. The JH III selected in the application is a hormone secreted by the honeybee itself, has the characteristics of strong targeting and low toxicity to the honeybee, and has a precise application dose, and has no significant influence on the normal growth and development, reproduction and foraging behavior of the honeybee, and the quality and safety of the bee product.

The invention relates to the technical field of biology, and provides an application of a connexin signal channel in regulating and controlling the body weight and the body shape of worker bees. According to the invention, a cascade regulation pathway of Jhe-JH-Titin in bees is disclosed for the first time, which indicates that Jhe (juvenile hormone esterase) regulates JH (juvenile hormone) and further regulates the expression of Titin (connexin) to influence the growth and development of bees, and finally regulates the body type and weight of worker bees of bees. By means of the method, the purposes of regulating and controlling the birth weight and the body type of worker bees can be achieved. According to the method, molecular breeding work is carried out by adopting the regulation and control effect of the Jhe-JH-Titin pathway on the body type of the bees, the traditional breeding period can be shortened, and the breeding target of improving the body type of the bees can be rapidly achieved. The method is simple, convenient, easy to operate and short in breeding period, the body length of worker bees can be rapidly increased only through one generation, the breeding cost can be effectively reduced, and the method has important application and popularization value for bee breeding.

An insecticidal and acaricidal composition of the present invention comprises an active component A and an active component B, the ratio of weight parts of the active component A to the active component B being 1:100-100:1. The active component B is selected from one or more of bionic juvenile hormones, chitin biosynthesis inhibitors that affect CHS1, type-1 chitin biosynthesis inhibitors, ecdysone receptor agonists, nicotinic acetylcholine receptor competitive modulators, ryanodine receptor modulators, nicotinic acetylcholine receptor allosteric modulators, glutamate-gated chloride channel allosteric modulators, sodium channel modulators, voltage-dependent sodium channel blockers, inhibitors, trifluoroethyl sulfide acaricides and other insecticides and acaricides. The active component A is selected from a compound (I), a compound (II) having the following structures, or stereoisomers thereof. The insecticidal and acaricidal composition of the present invention has the advantages such as obvious synergistic effect and delayed development of drug resistance, and can be used for controlling a variety of pests.

The application provides a CYP450 family gene Mcic000159 gene involved in cantharidin biosynthesis, the nucleotide sequence of the Mcic000159 gene is shown as SEQ ID NO. 1, and the coded amino acid sequence is shown as SEQ ID NO. 2. When the expression of the CYP450 family gene Mcic000159 gene in the application is interfered in the body of the L. decemnotata larva, the content of juvenile hormone III in the body of the L. decemnotata larva has no influence, the content of cantharidin is reduced, and the relative expression amount of the upstream genes McicHMGR, McicFPPS and McicJHEH of cantharidin synthesis is up-regulated. The CYP450 family gene Mcic000159 gene in the application acts on the metabolic product downstream of juvenile hormone III to cantharidin, and fills the blank of the stage from juvenile hormone III to cantharidin in the cantharidin synthesis route.

The application belongs to the technical field of forestry protection and detection, and particularly relates to a large-scale propagation method of pinus koraiensis shoot bactra pestis parasitoid, which comprises the following steps: searching for pinus koraiensis shoot bactra pestis pupae based on a drone to generate a pinus koraiensis shoot bactra pestis pupae collection map; collecting pinus koraiensis shoot bactra pestis pupae based on the pinus koraiensis shoot bactra pestis pupae collection map, spraying juvenile hormone on the collected pinus koraiensis shoot bactra pestis pupae, putting the pinus koraiensis shoot bactra pestis pupae into a glass tube, and culturing the pinus koraiensis shoot bactra pestis pupae to pupae medium bees, and then storing the pupae medium bees at low temperature after the pupae medium bees are hatched; immersing the host pupae in a 3-5% sodium bicarbonate solution, taking out the host pupae, cleaning the host pupae with distilled water, spraying a layer of glucose oxidase on the surface of the host pupae, and naturally drying the host pupae at room temperature; and propagating the host pupae by using a glass tube bee propagation method or an open bee propagation method. The application can realize rapid searching of pinus koraiensis shoot bactra pestis pupae, thereby simplifying the collection process of pinus koraiensis shoot bactra pestis pupae, and significantly increasing the number of pinus koraiensis shoot bactra pestis parasitoid obtained by propagation.

The present invention relates to pesticide mixtures comprising as active compound I a carboxamide derivative active as an insecticide and at least one active compound II selected from group M comprising acetylcholine esterase inhibitors, GABA-regulated chloride channel antagonists, sodium channel modulators, nicotinic acetylcholine receptor agonists / antagonists, allosteric nicotinic acetylcholine receptor activators, chloride channel activators, juvenile hormone mimics, homopteran feeding blockers, mite growth inhibitors, mitochondrial ATP synthase inhibitors, oxidative phosphorylation uncouplers, chitin biosynthesis inhibitors, molting disruptors, ecdyson receptor agonists, octamine receptor agonists, MET inhibitors, voltage-gated sodium channel blockers, lipid synthesis inhibitors,ryanodine receptor modulators and other compounds defined in the description, in synergistically effective amounts. The invention also relates to methods and the use of these mixtures to combat and control insects, arachnids, or nematodes in plants, and to protect these plants against pest infestation, in particular also to protect plant propagation material, such as seeds.