The invention discloses a medical color doppler ultrasound coupling agent and a preparation method thereof, and belongs to the field of traditional Chinese medicines. Effective components of the coupling agent, namely a traditional Chinese medicine preparation, comprise the following raw materials: calendula, angelica dahurica, tea-seed oil, broom cypress fruit, lemna minor, leaves of acronychia pedunculata (L.) Miq., catalpa leaves, cinnamomum camphora leaves, delavay pararuellia herb, begonia fimbristipula, passiflora wilsonii Hemsl, potentilla fulgens Wall.ex Hook., cherry, milk, pinus koraiensis Sieb.et Zucc., and aloe. The herbs selected and used in the traditional Chinese medicine preparation are compatible, and the coupling agent conforms to the traditional Chinese medicine and modern medicine theories, has efficacies of clearing heat to remove toxicity, sterilizing and diminishing inflammation, and is excellent in lubrication effect, convenient to use, excellent in absorption effect, and free of adverse, toxic and side effects; clinical application proves that the coupling agent is non-irritant to a human body, excellent in imaging effect, high in image resolution, and suitable for clinical popularization and application to medical color doppler ultrasound diagnosis.