16 results about "Pinus koraiensis" patented technology

The invention relates to the technical field of cultivation of wild plants, in particular to a seedling raising method, a seedling raising device and a using method of extremely endangered plant pinus koraiensis, and the seedling raising method comprises the steps of seed collection and treatment, seed preparation and inspection, seed germination acceleration, nursery land selection, seedling cultivation, seedling management, fertilization and the like. The seedling raising container is a specially-designed seedling raising device. The seedling culture device comprises a seedling culture box and a spray irrigation box, a plurality of placement plates are arranged in an inner cavity of the seedling culture box, supporting plates are fixedly connected to the front and rear side walls of the placement plates, the side walls of the sides, away from the placement plates, of the supporting plates are fixedly connected with the inner wall of the seedling culture box, trays are arranged on the top surfaces of the placement plates, and a plurality of draining holes are formed in the bottom surfaces of the trays. The method can effectively protect endangered plants, enlarge the number of seedlings, and prevent species from going to eradicate.

The invention relates to a prediction method, in particular to a pinus koraiensis pine cone yield prediction method which comprises the following steps: S1, unmanned aerial vehicle oblique photography, S2, image processing, S3, orthographic splicing, S4, pine cone recognition and S5, yield prediction. According to the method, a large area can be covered in a short time, Korean pine image data can be rapidly acquired, indoor data analysis is carried out, the acquisition cost is only one tenth of that of traditional manual investigation, and almost no personnel safety risk exists. According to the method, the unmanned aerial vehicle is adopted to collect data, the efficiency is high, investigation can be carried out in a slightly allowed aerial photography window period in weather, the flexibility is high, the influence of topographic elements and accumulated snow elements is small, and the adaptability is high. The method comprehensively investigates the measurement area, identifies the positions and number of pinecones through an algorithm, greatly reduces manual intervention, and improves the investigation precision.

This invention relates to the field of plant genetic engineering technology, and in particular to a red pine. PkSHR Genes and their application in regulating somatic embryogenesis in Korean pine. The Korean pine mentioned... PkSHR The nucleotide sequence of the gene is shown in SEQ ID No. 1. The Korean pine provided by this invention... PkSHR Gene regulation of somatic embryogenesis in Korean pine, overexpression of Korean pine PkSHR The gene significantly improves the yield and quality of Korean pine embryos and promotes the growth of Korean pine seedlings.

The invention discloses application of cobalt chloride in promoting Korean pine somatic embryogenesis, and belongs to the technical field of plant regeneration. The invention aims to improve the somatic embryo generation efficiency and somatic embryo quality of the Korean pine callus. The invention provides an application of cobalt chloride in promoting Korean pine somatic embryogenesis. The concentration of the cobalt chloride is 50 mu M / L. According to the method, the genetic improvement efficiency of the Korean pine is improved.

The invention discloses a grafting method with a high survival rate of Pinus sylvestris var. mongolica grafted onto Pinus koraiensis in arid areas, comprising: selecting a Pinus sylvestris var. mongolica rootstock with a growth period of 4-6 years, flatly cutting below the terminal bud branch point to form a cross section, obliquely cutting two knives downward from the cross section to form a triangular pyramid-shaped mosaic structure, intercepting a semi-lignified Pinus koraiensis scion with a terminal bud, retaining the main bud and removing the lateral buds, obliquely cutting two triangular xylem sections from below the spore to form a double-bevel wedge-shaped joint body; when cutting the stock and the scion, immersing the cutting blade in an aqueous solution containing a plant growth regulator and a bactericide; inserting the double-bevel wedge-shaped joint body into the triangular pyramid-shaped mosaic structure so that the scion pith layer overlaps the stock pith layer; wrapping the interface with a composite hydrogel film comprising a thermosensitive polymer and an antibacterial component; and performing light shading after the grafting is completed. The present invention can significantly improve the survival rate of grafted seedlings.

The utility model relates to the technical field of grafting and fixing, and discloses a Korean pine grafting and fixing device which comprises two main body fixing rings, an inner top ring, a grafting body fixing ring, a locking plate and a supporting rod. According to the Korean pine grafting fixing device, a bolt penetrates through first connecting lugs at the ends of two main body fixing rings to form a closed ring, a threaded rod is rotated to adjust the position of an inner top ring, the inner top ring cooperates with an arc plate to be clamped to the surface of a tree trunk, and therefore the main body fixing rings are fixed to the surface of a tree; the supporting cylinder is inserted into soil, a tree can be supported, the possibility that the tree is bent during growth is effectively avoided, meanwhile, according to the position of a grafting branch, the height of the grafting body fixing ring is adjusted, the locking plates are moved, the two locking plates are matched with each other to clamp and fix the grafting branch, the grafting branch and a trunk of the tree can be fixed, and therefore the grafting effect is improved. The height of the fixed position is adjusted according to the grafting position.

The invention relates to the technical field of plant genetic engineering, in particular to a pinus koraiensis PkDof5.6 gene and application of the pinus koraiensis PkDof5.6 gene to regulation and control of pinus koraiensis somatic embryogenesis. The nucleotide sequence of the PkDof5.6 gene of the Korean pine is as shown in SEQ ID No. 1 (Sequence Identifier Number 1). The Korean pine PkDof5.6 gene provided by the invention regulates Korean pine somatic embryogenesis, and overexpression of the Korean pine PkDof5.6 gene significantly improves the yield and quality of the Korean pine somatic embryogenesis, and promotes the growth of Korean pine somatic embryogenesis seedlings. The method has important significance in optimizing a Korean pine somatic embryogenesis system and realizing efficient large-scale propagation.

This invention discloses an NNT activating composition comprising Dalbergia odorifera bark extract, Pinus koraiensis bark extract, and ascorbate tetraisopalmitate, wherein the mass ratio of Dalbergia odorifera bark extract, Pinus koraiensis bark extract, and ascorbate tetraisopalmitate is 1–15:1–2.5:2–50. This invention is characterized by its effective activation of NNTs.

The invention discloses a method for cultivating tricholoma matsutake by using sorghum straws, which comprises the following steps of: preparing a culture material by using the sorghum straws as a main material and wood chips, corncobs and lime as auxiliary materials through optimized three-stage fermentation, and cultivating tricholoma matsutake by combining a specific shed frame design, a compartment surface structure, a plum blossom hill-seeding method and a staged temperature, humidity and ventilation regulation and control strategy. The efficient cultivation of tricholoma matsutake is realized. The method effectively solves the problem of resource utilization of the sorghum straw, the cultivation process is clear, the cost is low, the method is particularly suitable for weather conditions in southwest China, the produced tricholoma matsutake is excellent in quality, the yield per mu can reach 6530 jin, the harvested mushroom residues can be directly returned to the field, and a green ecological cycle mode is formed.

The application discloses application of flavonoids in promoting embryogenic maintenance of Pinus koraiensis callus and somatic embryogenesis of Pinus koraiensis and belongs to the technical field of plant tissue regeneration. The application provides application of flavonoids in promoting embryogenic maintenance of Pinus koraiensis callus, somatic embryogenesis of Pinus koraiensis and reduction of active oxygen, wherein the flavonoids are quercetin, naringenin or catechin. The method improves the genetic improvement efficiency of Pinus koraiensis.

The utility model relates to the technical field of pinus koraiensis grafting, in particular to a pinus koraiensis grafting scion protection sleeve which comprises a protection cylinder, a fixing round hole is formed in the position, close to the bottom, of the outer side of the protection cylinder, a fixing bent rod is movably connected to the fixing round hole in a clamped mode, and a round baffle is fixedly installed on the position, close to the top, of the inner ring surface of the protection cylinder. An exposed circular hole is formed in the center of the top of the circular baffle, after grafting of the Korean pine is completed through thin film winding, the protection structure is driven by the pushing structure to clamp and protect the Korean pine, when wind and rain come, the circular baffle and the circular sponge are matched to block rainwater, the stability of the protection barrel is improved through the multiple fixing bent rods, and meanwhile the protection barrel is protected. Meanwhile, the protection structure in the protection barrel protects the red pine grafting position, the phenomenon that the red pine grafting position loosens or shifts under the scouring of wind and rain, and consequently the film is broken or falls off is avoided, the red pine grafting position is effectively protected, and the survival rate of red pine grafting is increased.

The invention discloses oplopanax elatus nakai compound essential oil with bacteriostatic activity and a preparation method of the oplopanax elatus nakai compound essential oil. The main components of the oplopanax elatus nakai essential oil comprise oplopanax elatus nakai essential oil, pinus koraiensis essential oil, pogostemon cablin essential oil, lavender oil, geranium essential oil, jojoba oil and grape seed oil. The preparation method comprises the following steps: firstly, selecting leaves of oplopanax elatus nakai with relatively high effective components from a cultivation base of oplopanax elatus nakai in Changbai county of Jilin province, then extracting oplopanax elatus nakai volatile oil by adopting a steam distillation method recorded in pharmacopoeia, and finally, adding other essential oil and base oil to prepare the oplopanax elatus nakai compound essential oil. The compound essential oil has bacteriostatic activity.

The application belongs to the technical field of forestry protection and detection, and particularly relates to a large-scale propagation method of pinus koraiensis shoot bactra pestis parasitoid, which comprises the following steps: searching for pinus koraiensis shoot bactra pestis pupae based on a drone to generate a pinus koraiensis shoot bactra pestis pupae collection map; collecting pinus koraiensis shoot bactra pestis pupae based on the pinus koraiensis shoot bactra pestis pupae collection map, spraying juvenile hormone on the collected pinus koraiensis shoot bactra pestis pupae, putting the pinus koraiensis shoot bactra pestis pupae into a glass tube, and culturing the pinus koraiensis shoot bactra pestis pupae to pupae medium bees, and then storing the pupae medium bees at low temperature after the pupae medium bees are hatched; immersing the host pupae in a 3-5% sodium bicarbonate solution, taking out the host pupae, cleaning the host pupae with distilled water, spraying a layer of glucose oxidase on the surface of the host pupae, and naturally drying the host pupae at room temperature; and propagating the host pupae by using a glass tube bee propagation method or an open bee propagation method. The application can realize rapid searching of pinus koraiensis shoot bactra pestis pupae, thereby simplifying the collection process of pinus koraiensis shoot bactra pestis pupae, and significantly increasing the number of pinus koraiensis shoot bactra pestis parasitoid obtained by propagation.

The invention relates to the technical field of forestry planting equipment, in particular to a Korean pine seed dibble seeding device which comprises a seed inlet pipe, a guide pipe, a ground breaking head, a first driving assembly and a second driving assembly. The guide pipe is fixedly arranged at the bottom of the seed inlet pipe, and the soil breaking head is arranged below the guide pipe. A seed inlet groove is formed in the side wall of the soil breaking head, a soil baffle is fixedly arranged on the bottom surface of the guide pipe, and the soil baffle corresponds to the seed inlet groove in position. A gap exists between the top of the soil retaining plate and the top of the seed feeding groove, the bottom of the soil retaining plate is attached to the bottom of the seed feeding groove, and a seed feeding channel is arranged in the seed feeding pipe. The first driving assembly is arranged on the inner wall of the top of the seed inlet pipe and used for driving the soil breaking head to move up and down. The second driving assembly is arranged above the seed inlet pipe and is used for driving the seed inlet pipe to move up and down. After the seed inlet pipe moves downwards, the guide pipe and the ground breaking head can make pits in the ground. And under the condition that the position of the soil breaking head is kept unchanged, the Korean pine seeds can fall into the hole with the predicted depth, so that the normal growth of the seeds is ensured.

The invention provides a Trichoderma atrobrunneum strain, a fermentation broth of the Trichoderma atrobrunneum strain, a fermentation broth extract of the Trichoderma atrobrunneum strain and application of the Trichoderma atrobrunneum strain to pine wood nematode resistance. The trichoderma atrophaeum is obtained by taking pinus koraiensis tissue of bursaphelenchus xylophilus as a separation object and culturing the pinus koraiensis tissue. The fermentation liquor and the fermentation liquor extract of the trichoderma atrophaeum have high lethal activity on pine wood nematodes, the 24-hour fatality rate of the fermentation liquor on the pine wood nematodes is 100%, and the 24-hour fatality rate of the active extract on the pine wood nematodes is 92 + / -13%. The strain contained in the fermentation liquor and the fermentation liquor extract for resisting pine wood nematode provided by the invention comes from pinus koraiensis tissue infected with pine wood nematode disease, is friendly to ecological environment and free of pollution, can be used for preparing corresponding products capable of being practically applied, and has huge application potential in the field of pesticides.