5 results about "Pinus koraiensis" patented technology

This invention relates to the field of plant genetic engineering technology, and in particular to a red pine. PkSHR Genes and their application in regulating somatic embryogenesis in Korean pine. The Korean pine mentioned... PkSHR The nucleotide sequence of the gene is shown in SEQ ID No. 1. The Korean pine provided by this invention... PkSHR Gene regulation of somatic embryogenesis in Korean pine, overexpression of Korean pine PkSHR The gene significantly improves the yield and quality of Korean pine embryos and promotes the growth of Korean pine seedlings.

The invention discloses application of cobalt chloride in promoting Korean pine somatic embryogenesis, and belongs to the technical field of plant regeneration. The invention aims to improve the somatic embryo generation efficiency and somatic embryo quality of the Korean pine callus. The invention provides an application of cobalt chloride in promoting Korean pine somatic embryogenesis. The concentration of the cobalt chloride is 50 mu M / L. According to the method, the genetic improvement efficiency of the Korean pine is improved.

The invention relates to the technical field of plant genetic engineering, in particular to a pinus koraiensis PkDof5.6 gene and application of the pinus koraiensis PkDof5.6 gene to regulation and control of pinus koraiensis somatic embryogenesis. The nucleotide sequence of the PkDof5.6 gene of the Korean pine is as shown in SEQ ID No. 1 (Sequence Identifier Number 1). The Korean pine PkDof5.6 gene provided by the invention regulates Korean pine somatic embryogenesis, and overexpression of the Korean pine PkDof5.6 gene significantly improves the yield and quality of the Korean pine somatic embryogenesis, and promotes the growth of Korean pine somatic embryogenesis seedlings. The method has important significance in optimizing a Korean pine somatic embryogenesis system and realizing efficient large-scale propagation.

The application belongs to the technical field of forestry protection and detection, and particularly relates to a large-scale propagation method of pinus koraiensis shoot bactra pestis parasitoid, which comprises the following steps: searching for pinus koraiensis shoot bactra pestis pupae based on a drone to generate a pinus koraiensis shoot bactra pestis pupae collection map; collecting pinus koraiensis shoot bactra pestis pupae based on the pinus koraiensis shoot bactra pestis pupae collection map, spraying juvenile hormone on the collected pinus koraiensis shoot bactra pestis pupae, putting the pinus koraiensis shoot bactra pestis pupae into a glass tube, and culturing the pinus koraiensis shoot bactra pestis pupae to pupae medium bees, and then storing the pupae medium bees at low temperature after the pupae medium bees are hatched; immersing the host pupae in a 3-5% sodium bicarbonate solution, taking out the host pupae, cleaning the host pupae with distilled water, spraying a layer of glucose oxidase on the surface of the host pupae, and naturally drying the host pupae at room temperature; and propagating the host pupae by using a glass tube bee propagation method or an open bee propagation method. The application can realize rapid searching of pinus koraiensis shoot bactra pestis pupae, thereby simplifying the collection process of pinus koraiensis shoot bactra pestis pupae, and significantly increasing the number of pinus koraiensis shoot bactra pestis parasitoid obtained by propagation.