86 results about "Bursaphelenchus xylophilus" patented technology

Provided are a lycoris radiata bulb extractive pesticide and a method for preparing an extractive. Lycoris radiata bulbs can be manufactured into the lycoris radiata bulb extractive pesticide in forms of missible oil, microemulsion, wettable powder and the like through certain processing procedures. The lycoris radiata bulb extractive pesticide has good insecticidal ovicidal activities to tetranychus, plutella xylostella, vegetable aphides, armyworms and pine wood nematodes and protective effect in fields and woodlands. The pesticide is remarkable in effect, low in toxicity and residuals, simple in preparing process, low in cost and suitable for popularization and application and accords with the development tendency of existing pesticides, and pests do not easily produce drug resistance.

The invention relates to a control technology specially used for preventing and controlling important forest pest bursaphelenchus xylophilus (monochamus alternatus), namely a microcapsule powder agent or a microcapsule suspending agent prepared by a highly-efficient, harmfulless and less-persistent neonicotinoid insecticide thiacloprid sustained-release preparation and assisted by a high-adhesionadhesive is adopted for prevention and control. The technology adopts an innocuous and degradable carrier as shell materials, is safe to environment, has excellent durable effect and solves the greatproblems of bursaphelenchus xylophilus prevention and control and chemical pesticide pollution.

The invention discloses a nematicidal composition and application thereof. The composition comprises active ingredients A and B, and a mass ratio of A to B is 20:1 to 1:20. The ingredient A refers to one of fosthiazate, abamectin, furfural, dazomet, fluopyram, carbosulfan, metham, verticillium chlamydosporium, emamectin benzoate, paecilomyces lilacinus or fluensulfone; and the structural formula of the ingredient B is a formula I or a formula II, wherein the formula I is as shown in the specification, and the formula II is as shown in the specification. The nematicidal composition disclosed by the invention is used for killing plant parasite nematodes comprising root-knot nematodes, ditylenchus destructor, heterodera sachachtii, pratylenchus coffeae and bursaphelenchus xylophilus, is composed of a novel nematicide containing lactonic rings and the existing insecticides and bactericides, and has good nematocidal activity and low toxicity to people and livestock. The nematicidal composition disclosed by the invention has an excellent effect of controlling eggs and second-stage larvae of the root-knot nematodes.

The invention relates to application of ethyl palmitate in preventing and controlling pine wilt disease. A method provided by the invention for preventing and controlling the pine wilt disease by using ethyl palmitate is that: the ethyl palmitate is placed into pine trees which contain generative bursaphelenchus xylophilus. The method provided by the invention can be used for treating pine trees which are infected with bursaphelenchus xylophilus in a forest. The problems of mass reproduction and rapid diffusion of bursaphelenchus xylophilus under a bursaphelenchus xylophilus epidemic situation can be solved.

The invention discloses a method for detecting bursaphelenchus xylophilus, a detection primer and a LAMP (loop-mediated isothermal amplification) detection kit of the detection primer. The method for detecting the bursaphelenchus xylophilus comprises the steps of (1) extracting a DNA (deoxyribonucleic acid) of the bursaphelenchus xylophilus in a pine wood sample; (2) constructing a LAMP reaction system for loop-mediated isothermal amplification; (3) judging whether the bursaphelenchus xylophilus exists in the pine wood sample according to the change of fluorescence or turdity of an amplified product. The invention also provides a method which is easy to operate, low in cost and high in stability and is used for extracting the DNA of the bursaphelenchus xylophilus from pine wood. The invention further provides a LAMP primer group with high specificity and high sensitivity for detection of the bursaphelenchus xylophilus. The invention also provides the LAMP detection kit for the bursaphelenchus xylophilus. The LAMP detection kit comprises the LAMP primer group, a reaction buffering solution, a DNA chain replacement enzyme and a fluorescent dye/specific probe. The detection kit disclosed by the invention has the advantages of high specificity, high sensitivity, convenience and quickness in detection and the like.

The invention belongs to the technical field of bursaphelenchus xylophilus invasion risk prediction, and discloses a bursaphelenchus xylophilus invasion risk prediction method based on an ecological niche factor model, and the method comprises the steps: obtaining distribution point data and environment data of an epidemic area of bursaphelenchus xylophilus; performing Kriging interpolation processing on the climate data, calculating the gradient and the slope direction in the topographic data, and obtaining vegetation types and human activity interference data; importing the distribution point data and various environmental data into an ecological niche factor model, and calculating pine wood nematode disease risk early warning; and evaluating the pine wood nematode disease invasion riskbased on the feature matrix and the risk map obtained by calculation. According to the invention, main environmental influence factors influencing pine wood nematode propagation are used; the method is suitable for local conditions, lays a foundation for early warning, monitoring and control work of pine wood nematode diseases and insect pests, provides a scientific basis for measures of forest biodiversity protection and ecological environment protection, and provides a basis and a reference for early warning and control work of forest diseases and insect pests.

The invention provides Candida with a nematocidal activity as well as a preparation method and application of Candida and belongs to the technical field of microbial pesticides. The Candida is Candida krusei TY9 and is preserved in the typical culture preservation center in China, and the preservation number of the Candida is CCTCCNO: M2014435. The Candida krusei TY9 strain is prepared by fermentation culture of conventional liquid, is applied to prevention and control of plant-parasitic nematodes, and particularly has a good effect of preventing and controlling panagrellus redivivus and/or bursaphelenchus xylophilus. The Candida has the advantages of low cost, simple preparation process, easiness in raw material obtaining, good in effect of killing nematodes and the like, and is suitable for industrial popularization and application.

The invention discloses a rice medium formula and culture method of Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola, and belongs to the technical field of microorganisms. Compared with a conventional PDA solid culture method and a PDB liquid culture method, a rice medium preparation and culture method of the Bursaphelenchus xylophilus endoparasitic fungus Esteya vermicola not onlyshortens the culture time of the bacteria, but also obtains a high-purity, high-density fungal spore suspension. The method is applied to the control of Bursaphelenchus xylophilus, and has significant economic, ecological and social benefits.

The invention discloses protein BxCDP1 of bursaphelenchus-xylophilus pathogen-associated molecular patterns and application thereof. The amino acid sequence of the protein BxCDP1 of the pathogen-associated molecular patterns (PAMPS) is shown in SEQID NO.2. From effectors secreted by bursaphelenchus xylophilus and the PAMP, the defensive responses of a host plant, namely, a pine tree, to bursaphelenchus xylophilus invasion are studied, the protein BxCDP1 of the pathogen-associated molecular patterns is obtained from the bursaphelenchus xylophilus, it is proven through experiments that the protein BxCDP1 can trigger cell necrosis of various plants including the host plant, has a certain broad spectrum in cell necrosis triggering, and stimulates the defensive response of the host plant. The BxCDP1 triggered cell necrosis depends on a co-receptor BAK1 of pattern recognition receptors, the BxCDP1 can stimulate the accumulation of nicotiana benthamiana ROS and up regulation of PTI Marker genes, and the immunoreaction of the nicotiana benthamiana is stimulated. It can be seen that the BxCDP1 is a PAMP secreted by the bursaphelenchus xylophilus, and has important theoretical and practicalsignificance for revealing the pathogenic mechanism of the bursaphelenchus xylophilus and improving the resistance of the pine tree to the bursaphelenchus xylophilus in a targeted mode.

The invention discloses a method for storing a monochamus alternatus hope sample and a method for fast detecting bursaphelenchus xylophilus therein. The method comprises the following step of putting the caught monochamus alternatus hope sample into 75% ethyl alcohol or a refrigeratory with -70 DEG C to be stored, thereby furthest preserving the amount of the bursaphelenchus xylophilus carried bythe monochamus alternatus hope. The invention creates a production technology of the bursaphelenchus xylophilus DNA template in the monochamus alternatus hope with the storing state, directly freezesand thaws lyses and centrifuges the middle and back thoracic tracheal tissues of the adult monochamus alternatus hop for PCR amplification, has the amplification rate of 100%, fastens the detecting speed, improves the detecting accuracy, and achieves the aim of convenient, fast and accurate detection.

The invention belongs to the technical field of tissue culture and discloses a method for ultralow-temperature preservation of anti-bursaphelenchus-xylophilus pinus massoniana embryonic callus. By proper pretreatment and adoption of a mixed cryoprotectant, the method is suitable for freeze preservation of the anti-bursaphelenchus-xylophilus pinus massoniana embryonic callus, cell viability of theembryonic callus unfrozen at 30-35 DEG C reaches 100% maximally, and the embryonic callus subjected to ultralow-temperature preservation has no evident difference from embryonic callus subjected to normal proliferation in appearance and microstructure and still has a function of differentiation for forming somatic embryos. An ultralow-temperature preservation system of the anti-bursaphelenchus-xylophilus pinus massoniana embryonic callus is established preliminarily, and technical references are provided for long-time embryonic maintaining of the resistant pinus massoniana embryonic callus.

The invention discloses primers, kits and a method for fluorescent quantitative PCR detection of bursaphelenchus xylophilus or bursaphelenchus mucronatus, wherein the fluorescent quantitative PCR detection primer for bursaphelenchus xylophilus comprises a PCR amplification primer I; the PCR amplification primer I comprises a PCR amplification forward primer I with the nucleotide sequence shown inSEQ ID NO: 1 (5'-GTTCCGCCTACTGATGGTTC-3') and a PCR amplification reverse primer I with the nucleotide sequence shown in SEQ ID NO: 2 (5'-CGGCTTTTCATCATCCTACGC-3'); the fluorescent quantitative PCR detection primer for bursaphelenchus mucronatus comprises a PCR amplification primer II; and the PCR amplification primer II comprises a PCR amplification forward primer II with the nucleotide sequenceshown as SEQ ID NO: 4 (5'-TTCTCTACGCAGCACGTTTGTCCG-3') and a PCR amplification reserve primer II with the nucleotide sequence shown as SEQ ID NO: 5 (5'-TTCACGAGACAGAGCTCACAACAAA-3'). The invention hasthe advantages of good DNA extraction effect, simple operation, short time consuming (within 1 hour), accurate and reliable results, and at least a single nematode can be detected, with the characteristics of high sensitivity and high specificity.

The invention discloses a monochamus alternatus repellent. The repellent comprises in percent by weight: 10-30% of p-allylanisole, 60-80% of a solvent and 5-10% of a synergist. The invention also discloses a preparation method of the monochamus alternatus repellent. The beneficial effects are as follows: the monochamus alternatus in the pine forest can be significantly repelled by the synergisticaction of the active components with good repelling effects, the carrying and spreading of bursaphelenchus xylophilus is reduced, the survival rate of pine trees is improved, the flooding of bursaphelenchus xylophilus can be effectively prevented and treated, and the transmission path is cut off. The biological prevention and treatment concept is used, the preparation of the repellent is simple and convenient, the cost is low, the resistance to monochamus alternatus cannot be caused for the long-term use, and thus the repellent is suitable for the hanging and slow-release use in a large area in the pine forest.

The invention discloses a method for evaluating the resistance of pine tree calli to bursaphelenchus xylophilus. The method is characterized in that the sterile bursaphelenchus xylophilus is utilizedfor being inoculated to induced pine tree embryogenic cell lines, the responses of different cell lines to the bursaphelenchus xylophilus are compared through the appearance shape change of the calli,a TTC cytochemical staining result and a bursaphelenchus xylophilus population increase speed, thus the resistant cell lines are sifted out, the sensitive cell lines are eliminated, and early-stage evaluation on the resistance of the pine tree embryogenic cells to the bursaphelenchus xylophilus can be conducted in the callus stage. According to the method, the sterile bursaphelenchus xylophilus is utilized for being inoculated to the induced pine tree embryogenic cell lines, the responses of different cell lines to the bursaphelenchus xylophilus are compared through the appearance shape change of the calli, the TTC cytochemical staining result and the bursaphelenchus xylophilus population increase speed, thus the resistant cell lines are sifted out, the sensitive cell lines are eliminated, and early-stage evaluation on the resistance of the pine tree embryogenic cells to the bursaphelenchus xylophilus can be conducted in the callus stage. By means of the method, the resistance of thepine tree calli to the bursaphelenchus xylophilus is simply and quickly evaluated.

To extract and detect Bursaphelenchus xylophilus DNA without isolating Bursaphelenchus xylophilus from a piece of wood taken from a tree belonging to the genus Pinus and the like, provided are a method for extracting Bursaphelenchus xylophilus DNA from a collected piece of wood that includes Bursaphelenchus xylophilus, a LAMP primer set including primers that anneal to a specific region of Bursaphelenchus xylophilus DNA, and a method for detecting Bursaphelenchus xylophilus from a piece of wood by amplifying a DNA by a LAMP method using this primer set.

The invention discloses a pine wood nematode disease occurrence area intelligent identification method. Remote sensing image data and a deep learning technology are applied to the field of pine wood nematode disease monitoring. A pine wood nematode disease occurrence area semantic segmentation sample data set is constructed based on a remote sensing image, a UNet semantic segmentation model is constructed, the model is trained and optimized, and intelligent recognition of a pine wood nematode disease occurrence area is achieved. The method is simple in process and high in practicability, a new method is provided for intelligent monitoring of pine wood nematode diseases, and the method is suitable for the fields of forest pest and disease damage monitoring, deep learning image recognition and the like.

The invention belongs to the technical field of morphological and molecular combined detection, and discloses a morphological and molecular combined detection method for pine wood nematodes. The method comprises the steps of sample separation, morphological detection of the pine wood nematodes and molecular detection of the pine wood nematodes. A simple pine wood nematode separation device is designed in the invention; saprophytic nematodes and aphelenchoides nematodes are distinguished by adopting the presence or absence of stylets, and morphological identification is carried out according tothe shape difference of the tails and the tips of the female adults of pine wood nematodes and the quasi pine wood nematodes; molecular identification is carried out on the aphelenchoides nematodes,the pine wood nematodes and bursaphelenchus mucronatus by adopting a specific PCR technology; and complete detection technology system details are designed. The same pine wood sample is repeatedly sampled for three times during DNA extraction, so that accidental detection errors during sampling are avoided. The method meets requirements of forestry workers for rapid, efficient and accurate pine wood nematode detection, and provides timely and reliable detection means and technical support for general investigation of pine wood nematode diseases of forestry departments.