The invention discloses a cultivation method for making an endangered thousand-year-old tree rejuvenate. According to the method, in the early stage that the endangered thousand-year-old tree is aboutto wither, faces death and is near extinction, the environmental, climatic and cultural cultivation is used, pine forest resources and environments for wild plant growth are protected, habitats and growth sites of wild animals and plants are recovered, man-made excessive deforestation and disordered exploitation and trampling are inhibited, and the tree-protecting awareness and tree-growing behaviors of people are achieved. The technologies of soil protection, weir building and earth filling, tree shaping, branch pruning, tree holding, tree supporting, tree protection, fertilization, watering, seed breeding, seed harvesting, seed storage, seed germination acceleration, sapling cultivation, transplanting and planting, grafting and regeneration are utilized, and disease and pest control iswell conducted, so that the endangered thousand-year-old tree rejuvenates, and luxuriant propagation and growth of branches and leaves are achieved; the protection of the endangered thousand-year-oldtree is beneficial to carrying out scientific research in China and achieving the sustainability of human landscapes, people can enjoy good life, and the endangered thousand-year-old tree can be scientifically utilized valuably.