2 results about "Dna strand displacement" patented technology

This invention discloses a method for implementing a novel biomolecular controller based on DNA strand substitution, comprising: modeling the enzymatic protein hydrolysis process using abstract chemical reactions; constructing a Brink controller BC based on a covalently modified cycle CMC; constructing a covalently modified cycle CMC-D with a direct positive self-regulating PAR structure; obtaining an improved Brink controller BC-DPAR using abstract CRNs; constructing a covalently modified cycle CMC-I with an indirect PAR structure; obtaining an improved Brink controller BC-IPAR using abstract CRNs; constructing an enzymatic protein hydrolysis model based on CRNs using a DNA strand substitution reaction; constructing controllers BC, BC-DPAR, and BC-IPAR respectively through the DNA strand substitution mechanism; and constructing different control schemes based on the differences between the controllers according to the enzymatic protein hydrolysis model. This invention utilizes the DNA strand substitution mechanism to realize control schemes for the enzymatic protein hydrolysis process under different controllers, which improves the output effect of hydrolysis products to a certain extent and can achieve the expected output level more quickly.

The application provides a method for building a 9-bit parity check molecular switch circuit, and the steps are as follows: based on DNA strand displacement technology, the structure and reaction mechanism of the molecular switch circuit are described; the reaction rate and leakage rate are set, and the input signal of the molecular switch circuit and the concentration ratio of the molecular switch at each stage are optimized; the fan-in molecular switch circuit and the fan-out molecular switch circuit are set according to the molecular switch circuit; the 3-bit parity check molecular switch circuit is built based on the fan-in molecular switch circuit and the fan-out molecular switch circuit by using the switch canvas routing strategy, the 9-bit parity check molecular switch circuit is built by using three 3-bit parity check molecular switch circuits with the same structure; and the correctness of the 9-bit parity check molecular switch circuit is verified by using a Simbiology simulation platform. In the construction of a large-scale complex logic circuit, the application has a simple and unified DNA chain structure, a fast reaction speed and good stability, and provides a new scheme for the construction of a modular DNA circuit.