Phototonus logical circuit design method based on SST nanometer platform

Abstract

The invention belongs to the technical field of DNA nanometer, and relates to a phototonus logical circuit design method based on an SST nanometer platform. Fluorescent molecules are arranged on the SST nanometer platform through DNA self-assembly, the position of the fluorescent molecules is arrayed in the DNA chain displacement process, and a fluorescent molecule energy transfer path is formed among the reset fluorescent molecules. In serial design of the SST nanometer structure, a single chain taking part in follow-up DNA strand displacement reaction needs to extend a hairpin structure region, the extended sequence and specific system input chains take part in the strand displacement reaction, fluorescent molecule transfer systems are successfully built, and therefore the fluorescent molecules can be transferred at normal temperature. Configuration among the fluorescent molecules is controlled by uniting multiple fluorescent molecule transfer systems, and a phototonus molecular logical circuit is obtained by means of fluorescence resonance energy transfer technology.

Description

technical field [0001] The invention belongs to the field of DNA nanotechnology, and relates to a design method of an optically stimulated logic circuit based on an SST nanometer platform. Background technique [0002] Biological systems exploit the dynamic interactions between molecules to manage their complex behavioral processes, so the development of technologies to observe and manipulate these molecular interactions is crucial to advance smart imaging and smart therapeutics in vivo. To implement these technological approaches, molecular logic circuits are a useful tool that can control the course of molecular reactions at the molecular level. In conventional design, in order to realize large-scale complex logic circuits, it is often realized by cascading logic gates. Since this kind of circuit relies on the step-by-step reaction of DNA molecules through diffusion in a solution environment, it usually takes a longer calculation time. In contrast, in the control system o...

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Problems solved by technology

[0006] In the design of conventional photoactive molecular logic circuits, DNA double helix structure and DNA origami structure are often used as scaffolding platforms. The DNA double helix structure is simple, and fluorescent molecules are arranged linearly on the double helix structure, which is often impossible to achieve. Complex logical function; DNA origami structure is composed of a long scaffold chain and many staple chains, due to the limitation of the long scaffold chain structure, the modification density of its fluorescent molecules is not high

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