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Copper nanocluster synthesized by dna nanoribbon template method, synthesis method and application thereof

A technology of copper nanoclusters and nanobelts, applied in the field of DNA nanometers, can solve problems such as insufficient heat resistance

Active Publication Date: 2021-07-06
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The fluorescence intensity of existing dsDNA-CuNPs decays too quickly after its formation, and the heat resistance is insufficient. Therefore, it is necessary to develop copper nanoclusters with stable fluorescence intensity and better performance.

Method used

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  • Copper nanocluster synthesized by dna nanoribbon template method, synthesis method and application thereof
  • Copper nanocluster synthesized by dna nanoribbon template method, synthesis method and application thereof
  • Copper nanocluster synthesized by dna nanoribbon template method, synthesis method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Method for Synthesizing Copper Nanoclusters Using DNA Nanoribbon Template Method

[0034] Proceed as follows:

[0035] S1, prepare the following five DNA strands (artificially synthesized by Invitrogen Company) with a concentration of 100 μmol / L, for future use:

[0036] Staple 1-1: 5'-CAGCCCTGTAAGATGAAGATAGCGTATGAGTA-3', as shown in SEQ ID NO.1;

[0037] Staple 1-2: 5'-TATGAGCGCACAATGGTCGGATTCGATGAACG-3', as shown in SEQ ID NO.2;

[0038] Staple 1-3: 5'-GTAGTCGTTCAACTCGTATTCTCATATGCTCA-3', as shown in SEQ ID NO.3;

[0039] Scaffold strand 1-1: 5'-C A G G G C T G T A C T C A T A C G C T C A TA C GTTCATCACGACTACATACGAGT-3', as shown in SEQ ID NO.4;

[0040] Scaffold strand 1-2: 5'-T G A G A A T A C G A G T T G A G A A T C C GA C CATTGTGCGCTATCTTCATCTTA-3', as shown in SEQ ID NO.5;

[0041] S2, take 2 μL of each of the five DNA strands in S1, and then add 10 μL of tris-HAc solution with a concentration of 400 mmol / L, and 10 μL of Mg(Ac) with a concentration of 100 mmo...

Embodiment 2

[0046] In order to prove the successful synthesis of copper nanoclusters in the present invention, methods such as atomic force microscopy and fluorescence photometry have been used to prove that the steps are as follows:

[0047] S1. Stick the mica sheet to the round iron sheet with double-sided tape and tear off the mica sheet with tape to make the surface smooth.

[0048] S2. Take 2 μL of the copper nanocluster synthesized in Example 1 and drop it into the mica sheet prepared in S1, and then rinse the surface of the mica sheet with 100 μL of ultrapure water.

[0049] S3. Put the sample prepared in S2 into an atomic force microscope for characterization.

[0050] The result is as figure 2 As shown, the result proves that: the atomic force tries to show that he is a DNA nanobelt, a ribbon structure.

[0051] S4. Take 180 μL of the copper nanocluster synthesized in Example 1 and place it in a 700 μL cuvette, set the excitation wavelength to 351 nm for fluorescence scanning....

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Abstract

The invention relates to the field of DNA nanotechnology, more specifically, a copper nanocluster synthesized by a DNA nanobelt template method, a synthesis method and an application thereof. The present invention uses DNA origami to synthesize a DNA nanobelt, and synthesizes copper nanoclusters using the DNA nanobelt as a template. The copper nanoclusters of the present invention are arranged more neatly, have stable fluorescence intensity, strong heat resistance, and superior performance , which is expected to show greater potential in label-free fluorescent bioassays.

Description

technical field [0001] The invention relates to the field of DNA nanotechnology, more specifically, a copper nanocluster synthesized by a DNA nanobelt template method, a synthesis method and an application thereof. Background technique [0002] DNA origami technology uses the special base pairing mechanism of DNA to make DNA form highly ordered nanostructures through self-assembly, such as: complex two-dimensional objects, three-dimensional objects and surface patterns. With the increasingly perfect DNA origami technology, DNA is not only a kind of genetic material, but also a more complex structure and function of DNA has been developed. [0003] Nanoclusters of noble metals such as gold, silver, and copper refer to clusters formed by dozens of metal atoms, usually with a diameter of about 2nm. Due to their excellent fluorescence properties, ultra-fine size, good biocompatibility, and low toxicity, Noble metal nanoclusters have attracted extensive attention in in vivo imag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/16G01N21/64C09K11/58B82Y30/00B82Y40/00
CPCB22F9/16G01N21/6428G01N21/6486C09K11/58B82Y30/00B82Y40/00B22F2009/165C12Q1/68
Inventor 欧阳湘元王美芳任永安王健
Owner NORTHWEST UNIV
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