21 results about "Nanotechnology Techniques" patented technology

The invention relates to the technical field of biomedicine nanotechnology, in particular to a high-stability engineered exosome based on rigid hydrophobic anchoring and micelle disassembly and purification as well as a preparation method and application of the high-stability engineered exosome. According to the invention, a ternary surface structure of rigid hydrophobic anchoring-hydrophilic polymer spacer arm-targeting ligand (L-S-T) is constructed, cholesterol hemisuccinate or multi-branched lipid is selected as an anchoring group, and the problem that conventional phospholipid modification is easy to fall off in vivo and in an albumin-containing preparation is obviously solved; a technology of'micellar insertion after temperature control 'combined with'CMC critical concentration adjustment and purification' is adopted, lossless insertion of functional molecules is realized by utilizing thermodynamic driving force, and residual micelles are induced to be disintegrated into monomers by adjusting the concentration of a system to be lower than the concentration of critical micelles, so that efficient removal of free impurities is realized through conventional ultrafiltration. The engineered exosome has the advantages of long circulation, high targeting and excellent storage stability, and has important clinical application and commercial transformation prospects.

Provided is a sacrificial composition. The sacrificial composition comprises a polymer (A) that undergoes acid-catalyzed decomposition, a compound (B) that generates an acid by heat, and a solvent (C), wherein the compound (B) that generates the acid by heat consists of a carboxylic acid and a base, and a content of the compound (B) is 1 to 60 parts by mass based on 100 parts by mass of the polymer (A). The sacrificial composition may exhibit at least one of properties as an advanced material or as a high performance material. The sacrificial composition may be used in the nanotechnology process to make semiconductor device / display device application, for example semiconductor chip, or a liquid crystal, quantum dot, OLED display fabricated on a substrate controlled by semiconductors.

The invention relates to the technical field of spacecrafts, in particular to a spacecraft defense material and a preparation method thereof, and designs a structure capable of effectively absorbing and dispersing impact energy on microscopic and macroscopic scales by utilizing computer simulation and an advanced material modeling technology. A base material with high strength, high toughness, light weight and corrosion resistance is selected, nanofibers, nanoparticles or nanosheet layers are embedded into the base material by utilizing a nanotechnology so as to enhance the mechanical property and the impact resistance of the base material, and the shape, the structure and the performance of the material are accurately controlled by adopting an advanced manufacturing technology. The prepared materials are stacked, bonded or mechanically connected together to form a final defense structure, and the structure is strictly tested. By combining a nanotechnology, composite material engineering and an additive manufacturing defense material, the deep requirement of a space exploration task is met, and the safety of astronauts and the smooth completion of the task are ensured.

The present invention is in the field of materials engineering and nanotechnology. More specifically, the present invention discloses: a functional premix comprising nanoparticles and a carrier or compatibilizer; a method of obtaining the premix; a method of altering the properties of a material; and a modified material formed with improved properties. Unexpectedly, the premix of the present invention can be used to alter the properties of different kinds of materials, such as polymers, metals, ceramic materials, and / or composites of different materials. The premix of the present invention has numerous advantages in terms of transportation, use, and incorporation of nanoparticles into different materials. In one embodiment, the present invention provides significant modification of the properties of polymers and, in fact, breaks the existing concepts in this technical field. In another embodiment, the present invention provides advantages in altering the properties of metals and / or ceramic materials.

The invention discloses a molecular system based on DNA nano-structure artificial atoms and a construction and regulation method, and belongs to the technical field of DNA nanotechnology. The construction method of the molecular system comprises the following steps: mixing DNA chains with extension chains, adding a TM buffer solution, and carrying out annealing reaction to synthesize a DNA nano-structure with extension chains; dNA nanostructures are used for representing artificial atoms, extended chains on the DNA nanostructures are used for representing valence electrons, double chains formed by interaction among the extended chains are used for representing chemical bonds, a plurality of DNA nanostructures with extended chains are mixed in proportion according to a target molecular configuration, and a molecular system is obtained through one-pot assembly. Dynamic dissociation and recombination of artificial atoms are achieved based on DNA strand displacement reaction, and accurate regulation and control of a programmable molecular system are achieved. The construction precision and regulation effectiveness of the molecular system are verified through various technologies, the system has the advantages of being high in programmability, high in assembly efficiency, good in structural controllability and the like, and a brand new technical platform is provided for the fields of material design, chemical process simulation, biological engineering and the like.

This invention belongs to the field of biomedical materials and nanotechnology, specifically relating to the synthesis and application of a multifunctional mesoporous nanomaterial. The inventors synthesized a core-shell structured mesoporous nanoparticle using mesoporous silica nanoparticles and dopamine via emulsion epitaxy. The core of the core-shell structured mesoporous nanoparticle is S-nitrosoglutathione supported on mesoporous silica, and the outer shell is pentafluorophenyl chlorophyll supported on mesoporous polydopamine, forming MSN / G@mPDA / F nanoparticles. These nanoparticles rapidly increase in temperature to 45°C under 808 nm laser irradiation. o At temperatures above a certain temperature, S-nitrosoglutathione decomposes to produce nitric oxide. Further irradiation at 750 nm generates a large amount of reactive oxygen species, killing bacteria. This combination of photothermal and photodynamic therapy effectively inhibits bacterial growth. Therefore, this method exhibits excellent antibacterial effects and can play a role in the future treatment of bacterial infections.

FIELD: nanomaterials.SUBSTANCE: invention relates to chemical technology, nanotechnology and materials science and can be used in the production of highly efficient photocatalysts, materials for photoelectrochemical cells, sensors and functional coatings. First, laser ablation of the primary material, titanium nitride TiN, is carried out in a liquid, which is distilled or deionized water, resulting in the formation of a suspension of spherical TiN nanoparticles. The resulting nanoparticles are oxidized by heating the said suspension at 80-90 °C for 2-6 hours. Then centrifugation is performed for 20 minutes at a frequency of 15,000 rpm.EFFECT: obtaining nanocrystalline titanium dioxide flakes with a controlled content of the anatase phase of up to 90% and higher at atmospheric pressure in a neutral aqueous environment without the use of acids and morphological controlling agents, and also reducing the oxidation temperature.2 cl, 3 dwg

The invention discloses a targeted gating ratio type pH sensor of a double-CRISPR system based on tetrahedral framework nucleic acid, and belongs to the technical field of biomedicine and nucleic acid nanotechnology. According to the sensor, tetrahedral framework nucleic acid is used as a rigid support, Cas12a, Cas13a and corresponding fluorescent reporter molecules are accurately assembled according to the stoichiometric ratio of 1: 1, and orthogonal pH responses of the Cas12a and the Cas13a form a ratio type sensing basis; target recognition-pH sensing logic gating is realized through target specific crRNA (Complementary Ribonucleic Acid) design; after being modified by cholesterol, the compound can be stably anchored on the surface of a lipid membrane. The invention also discloses a preparation method of the sensor, a membrane anchoring method, a pH monitoring method and a target gating function realization method. The sensor has the advantages of high-precision ratio measurement, accurate spatial arrangement, high specificity, stable membrane anchoring, wide pH response range and the like, and can be used for accurately monitoring pH dynamics in a biological membrane interface microenvironment and extracellular vesicle fusion process.

This invention relates to the field of biodetection technology, specifically to a multimodal miRNA detection array based on Mn-CeO2@CDs nanozymes and Cas12a sensing, its preparation method, and its applications. The recognition and signal amplification probe composition based on Mn-CeO2@CDs nanozymes and Cas12a includes: a solid substrate, the DNA Y-shaped structure described in the first aspect, the composite nanozyme, and a CRISPR / Cas12a system. This invention combines DNA nanotechnology, Cas12a enzyme digestion technology, and nanozymes as signal output mediators, achieving highly specific and sensitive multimodal detection of myocarditis biomarkers (miRNAs) in fluorescence, UV-visualization, and electrochemical modes.

The present application relates to the field of nanotechnology, in particular to a raffinose-SS31 peptide multi-targeted functional chitooligosaccharide / astaxanthin nanoparticle and a preparation method and application thereof.The nanoparticle of the present application takes precise regulation at the nanometer scale, innovative application of new components and targeted synergistic delivery as the core design idea, constructs a multifunctional nanofunctional network, provides support for the nanoscale precise delivery of active ingredients such as astaxanthin, and at the same time, establishes a chitooligosaccharide nanoparticle preparation technology with high active substance loading rate and stability for the problems of low stability and poor efficacy of chitooligosaccharide encapsulated active substances;targeting ligands are introduced by structure modification to enhance the specific recognition and binding capacity of nanoparticles to specific cells and tissues, and a targeted delivery technology for site-specific release and precise delivery of active substances is established.

The present application relates to the field of nanoscience and nanotechnology, and particularly relates to a particle thermodynamic measurement method and device based on double laser confocal microscopy, in which a high-power continuous laser is used as a trapping laser to trap particles in a solution, and the movement of the particles in the trapping process is monitored in real time, the force acting on the particles is analyzed according to the movement trajectory of the particles, then a low-power continuous laser is used as a Raman detection laser to focus on the trapped particles to excite Raman signals, and Raman spectroscopy is used to quantify the temperature of the particles to perform particle thermometric measurement and realize thermodynamic measurement of the particles. The method of the present application can be used in the field of physics to study the thermodynamic coupling problem in optical tweezers trapping, can be used in the field of biology to achieve precise trapping of cells or microorganisms under low heat, and can be used in the field of energy utilization to realize efficient utilization of energy by precisely regulating the thermodynamic properties of micro-nano structures.

This invention discloses a microfluidic-based polypeptide-based helical fiber with circularly polarized luminescence and its preparation method, belonging to the field of nanotechnology. Utilizing multiple hydrogen bonding interactions between polypeptides and based on the solute conformational confinement and ordered solvent diffusion mechanism of a microfluidic laminar flow chip, this invention develops a polypeptide-based circularly polarized luminescent material with excellent luminescence performance. Circular dichroism spectroscopy and circular polarization spectroscopy tests show that the helical fiber material obtained by this invention exhibits mirror-symmetric signals. Compared with materials obtained by traditional solution stirring, the polypeptide-based helical fibers prepared in the microfluidic chip by this invention not only have a more uniform morphology but also a larger... g lum value( L -Peptide / TBI and D The luminescence asymmetry factor of -Peptide / TBI is ±1.9×10⁻⁶. ‑3 Furthermore, this method has a certain degree of universality and is applicable to the assembly of other macromolecules, including polymers, nucleic acids, and proteins.

The present application belongs to the cross field of molecular diagnosis, environmental microorganism detection and nanotechnology, and particularly relates to a biological aerosol automatic detection platform, a detection method and application. The present application realizes specific capture and enrichment of target microorganisms through a silicon nanotip array, and the silicon nanotip array chip is provided with a plurality of regularly arranged silicon nanotips, and the surface of the silicon nanotip is modified with biological capture molecules. The temperature change of the nanotip is monitored in real time by using Raman spectrum, and the feedback optimization of the lysis / amplification temperature curve is realized, the double signal amplification and verification of LAMP amplification and CRISPR detection are combined, the high-resolution melting curve is analyzed through a machine learning algorithm, and the automatic identification and molecular typing of the strain are realized. The present application has the advantages of high detection sensitivity, strong specificity, simple operation, short detection time and the like. The present application is suitable for on-site rapid detection and real-time monitoring of pathogenic bacteria in various environments such as atmosphere, exhaled air and water.

The application belongs to the field of drug delivery nanotechnology, and provides a method for preparing cubic lipid nanoparticles encapsulating small interfering ribonucleic acid and application thereof. The application adopts a trinity design of ion complex pre-assembly of succinylated glycerol monooleate and cationic lipids, space stabilization of poloxamer 407, and rapid particle formation by microfluidics, realizes stable construction of internal nanostructure of inverse bicontinuous cubic phase, encapsulation of small interfering ribonucleic acid in water channel network with high drug loading, long-term stability of colloids under physiological ion strength conditions, and excellent batch consistency, solves the technical contradictions of existing lipid nanometer delivery systems, such as difficulty in giving consideration to low polydispersion and batch consistency, mutual restraint of cationic complex strength and colloidal stability, and difficulty in synergistic optimization of high drug loading and stability of cubic phase structure under high solid content microfluidic conditions, and has wide application value in gene therapy and nucleic acid drug delivery.

The invention belongs to the field of nanotechnology, and relates to a method for constructing condensed fluid droplets based on tetrahedral framework nucleic acid, which comprises the following steps: dissolving and diluting DNA dry powder with enzyme-free water to obtain a DNA solution, mixing four chains A, B, C and D of the obtained tetrahedral framework nucleic acid in a 50mM TM buffer solution, and carrying out polymerase chain reaction to obtain a tetrahedral framework nucleic acid structure, performing thermal insulation water bath or cooling annealing treatment on the tetrahedral framework nucleic acid structure to obtain framework nucleic acid condensation liquid drops; according to the method, a tetrahedral framework nucleic acid structure with a palindromic sequence is assembled, cohesive end sequence self-association is carried out by utilizing a salt solution, the tetrahedral framework nucleic acid is assembled into micron-sized DNA condensed liquid droplets, and a new strategy is provided for exploring a functional liquid-phase multi-stage material according to the formation and dynamic properties of the DNA condensed liquid droplets.

The application discloses a coenzyme Q10-loaded nano organic metal framework material and a preparation method and application thereof, and relates to the field of biological macromolecular materials and nanotechnology. The preparation method comprises the following steps: (1) preparation of Al-MOF; (2) preparation of the coenzyme Q10-loaded nano carrier Al-MOF; and (3) preparation of the coenzyme Q10-loaded nano carrier Al-MOF@PEG. In the application, 2,5-dihydroxyterephthalic acid and aluminum (Al) ions are selected, Al-MOF nano carriers are synthesized through a solvothermal method, coenzyme Q10 is loaded into the interspace of the Al-MOF nano carriers, then mPEG5k-NH2, i.e. a kind of PEG with an amino group, is selected, and the carboxyl on the surface of the Al-MOF is activated by dicyclohexyl carbodiimide (DCC), and the amino at the end of the PEG is covalently modified to the surface of the nano carrier Al-MOF through “click chemistry” between the carboxyl and the amino. The nano carrier Al-MOF has a larger pore size, the drug loading capacity is increased, the water solubility is significantly increased after the introduction of the PEG, the circulation time of the nano carrier in a physiological environment is prolonged, and the bioavailability of the coenzyme Q10 is further improved.

The invention discloses a nucleic acid-metal oxide nanoparticle with a Yolk-Shell structure as well as a preparation method and application of the nucleic acid-metal oxide nanoparticle, and belongs to the crossing field of a nanotechnology and nano biomedicine. The method comprises the following steps: firstly, adding DNA into water, then adding a FeCl2. 4H2O solution to obtain a mixed solution, carrying out vortex treatment for 10 seconds, then carrying out metal bath reaction, cooling to room temperature after the reaction is finished, centrifuging, collecting a centrifugal precipitate, cleaning, and resuspending in deionized water to obtain a first solution; and adding hexamethylenetetramine into the first solution, rotating on a rotary shaking table, then reacting in a metal bath, cooling to room temperature after the reaction is finished, centrifuging, collecting centrifugal precipitate, and cleaning with deionized water to obtain the nucleic acid-metal oxide nanoparticles with the Yolk-Shell structure. According to the present invention, through the synergistic effect of the metal ion-DNA coordination driven self-assembly and the oxidation reduction process, the controllable construction of the Yolk-Shell structure nucleic acid-metal oxide nanoparticles is achieved; the prepared nucleic acid-metal oxide nanoparticles with the Yolk-Shell structure can be applied to preparation of nucleic acid delivery drugs.

The invention relates to the crossing field of functional polymer materials and biological nanotechnology, in particular to preparation of a self-alkali source intelligent polymer and application of the self-alkali source intelligent polymer in preparation of magnetic beads. Specifically, the self-alkali-source intelligent polymer is created for the first time, the molecular chain of the polymer synchronously integrates an alkaline functional group and a carboxyl precursor structure, and in the magnetic bead synthesis process, an alkaline reaction environment can be spontaneously constructed without additionally adding an alkali source to drive the generation of a magnetic nucleus; through simple post-treatment, the carboxyl precursor on the polymer can be efficiently converted into a carboxyl functional surface, so that the integrated preparation of the carboxyl functionalized magnetic bead is realized. Based on the intelligent polymer, the invention further provides a novel technology for preparing the magnetic beads through one-step nucleic acid extraction. According to the method, the technological process can be greatly simplified, the batch stability of the magnetic beads is effectively improved, and efficient and stable preparation of the nucleic acid extraction magnetic beads is promoted.

The invention discloses biomimetic catalytic nano-particles, a preparation method thereof and application of the biomimetic catalytic nano-particles in preparation of cerebral hemorrhage treatment drugs, and belongs to the technical field of biomedical nano-particles. The preparation method comprises the following steps: S1, preparing a mixed solution A by taking polybasic carboxylate and monobasic carboxylate as raw materials; s2, uniformly mixing a ceric ammonium nitrate solution, a heme oxygenase-1 solution and a deferoxamine solution to obtain a mixed solution B; s3, mixing and stirring the mixed solution A and the mixed solution B to prepare the biomimetic catalytic nanoparticles. The preparation method is simple and mild in condition, the prepared biomimetic catalytic nano-particles have efficient focus targeting performance, the multi-mechanism synergistic treatment effect of hematoma toxicity (removing heme, iron and ROS) and tissue repair (promoting angiogenesis and neuroprotection) is achieved at the same time, and the biomimetic catalytic nano-particles are remarkable in clinical treatment effect and excellent in biological safety and have good application prospects. Good clinical transformation prospects are realized.

The application belongs to the field of material science and nanotechnology, and particularly relates to a copper-based catalyst with a spatially separated tensile and compressive strain field. The catalyst comprises a copper matrix and nickel oxide pinning centers atomically dispersed on the surface of the copper matrix, wherein the copper matrix has spatially separated compressive strain regions and tensile strain regions, and the two types of strain regions are distributed at a nanoscale interval. The nickel oxide pinning centers are in an atomic dispersion state, form a stable Ni-Cu bonding structure with the copper matrix, can effectively anchor the strain field, and improve the stability of the material. The application adopts a wet chemical reduction method to construct a spatially separated tensile and compressive strain field in a single copper-based material, the local strain degree can be controlled, the preparation method is simple in process and mild in conditions, does not require special equipment, is easy to produce on a large scale, and has a good industrial application prospect.

This invention relates to a method for non-destructive treatment of two-dimensional material surfaces using a mass-selective gas cluster ion beam and to transistor devices thereof, belonging to the fields of microelectronics, nanotechnology, and two-dimensional material device manufacturing. This invention introduces a gas cluster ion beam integrated with a mass selector to treat the surface of two-dimensional materials before key process nodes in the two-dimensional material device fabrication process. By integrating the mass selector and gas atomic clusters, the cluster size is precisely screened, and the average energy of each cluster atom is controlled within a low-temperature range of approximately 1 eV. Utilizing the "low energy per atom" of the cluster ions, polymer residues on the two-dimensional material surface can be thoroughly removed while completely avoiding damage to its crystal structure. This method is compatible with standard photolithography processes, significantly reduces device contact resistance, and improves carrier mobility, providing a reliable surface treatment solution for the fabrication of high-performance two-dimensional material devices.