55 results about "Nanotechnology Techniques" patented technology

The invention discloses an SERS (Surface Enhanced Raman Scattering) substrate with a needle-like diagram nanostructure and a preparation method of the SERS substrate, and belongs to the field of nanotechnology and detection. The preparation method of the needle-like Turing nano-structure SERS substrate comprises the following steps: by taking a silver needle as a carrier, carrying out electrochemical etching on the surface of the silver needle to induce to form a Turing nano-structure with fractal topological characteristics, and then inducing to conformally grow a layer of zeolite imidazole framework-8 material as a shell layer by utilizing the nano curvature characteristics of the fractal structure, so as to prepare the needle-like Turing nano-structure SERS substrate. And a composite SERS enhanced platform with an electromagnetic field hot spot aggregation effect and a molecular screening and enriching capability is constructed. The platform has the advantages of high structural uniformity, expandability in manufacturing, synergy of signal enhancement and molecular recognition and the like, remarkably improves the detection sensitivity and the signal reproducibility, and is suitable for trace analysis in a complex sample system, such as related application in the fields of food safety, environmental pollution monitoring, biomedicine and the like.

The invention relates to the field of nano science and nano technology, in particular to a low-power particle capture and local thermal detection method and device based on tip-enhanced Raman optical tweezers, under laser irradiation, directional optical force is generated through an enhanced electric field between a nano tip and metal particles, and capture of the metal particles is achieved; raman scattering on the surface of the needle tip can be excited through focused laser, and the local temperature of the needle tip can be obtained by analyzing the change of a Raman characteristic peak. According to the method, directional optical force is generated through an enhanced electric field between the needle tip and the metal particles to capture the metal particles, the problem that the metal particles cannot be captured by single-beam 532 nm laser is solved, the power density of captured particles is reduced, and the adverse effect of the heat effect on capture is reduced. The method also can amplify the Raman signal of the needle tip to realize Raman detection of a local position, realizes temperature monitoring at the capture position, and lays a foundation for exploring the influence of the thermal effect on the capture process.

The invention relates to the technical field of biomedicine nanotechnology, in particular to a high-stability engineered exosome based on rigid hydrophobic anchoring and micelle disassembly and purification as well as a preparation method and application of the high-stability engineered exosome. According to the invention, a ternary surface structure of rigid hydrophobic anchoring-hydrophilic polymer spacer arm-targeting ligand (L-S-T) is constructed, cholesterol hemisuccinate or multi-branched lipid is selected as an anchoring group, and the problem that conventional phospholipid modification is easy to fall off in vivo and in an albumin-containing preparation is obviously solved; a technology of'micellar insertion after temperature control 'combined with'CMC critical concentration adjustment and purification' is adopted, lossless insertion of functional molecules is realized by utilizing thermodynamic driving force, and residual micelles are induced to be disintegrated into monomers by adjusting the concentration of a system to be lower than the concentration of critical micelles, so that efficient removal of free impurities is realized through conventional ultrafiltration. The engineered exosome has the advantages of long circulation, high targeting and excellent storage stability, and has important clinical application and commercial transformation prospects.

Provided is a sacrificial composition. The sacrificial composition comprises a polymer (A) that undergoes acid-catalyzed decomposition, a compound (B) that generates an acid by heat, and a solvent (C), wherein the compound (B) that generates the acid by heat consists of a carboxylic acid and a base, and a content of the compound (B) is 1 to 60 parts by mass based on 100 parts by mass of the polymer (A). The sacrificial composition may exhibit at least one of properties as an advanced material or as a high performance material. The sacrificial composition may be used in the nanotechnology process to make semiconductor device / display device application, for example semiconductor chip, or a liquid crystal, quantum dot, OLED display fabricated on a substrate controlled by semiconductors.

The invention relates to the technical field of spacecrafts, in particular to a spacecraft defense material and a preparation method thereof, and designs a structure capable of effectively absorbing and dispersing impact energy on microscopic and macroscopic scales by utilizing computer simulation and an advanced material modeling technology. A base material with high strength, high toughness, light weight and corrosion resistance is selected, nanofibers, nanoparticles or nanosheet layers are embedded into the base material by utilizing a nanotechnology so as to enhance the mechanical property and the impact resistance of the base material, and the shape, the structure and the performance of the material are accurately controlled by adopting an advanced manufacturing technology. The prepared materials are stacked, bonded or mechanically connected together to form a final defense structure, and the structure is strictly tested. By combining a nanotechnology, composite material engineering and an additive manufacturing defense material, the deep requirement of a space exploration task is met, and the safety of astronauts and the smooth completion of the task are ensured.

The present invention relates to water-sensitive carbon dots and a preparation method thereof, and relates to the field of carbon nanotechnology. The preparation method of the present invention is as follows: carbon source A and carbon source B are added to a solvent to obtain a mixed solution, the mixed solution is subjected to a hydrothermal reaction, the mixed solution is removed after the reaction is complete, the mixed solution is centrifuged to obtain a supernatant, the supernatant is mixed with water, and then centrifuged, and the filtrate is removed to obtain water-sensitive carbon dots. The present invention provides water-sensitive carbon dots, a preparation method thereof, and applications thereof. The water-sensitive carbon dots prepared by the present invention via a hydrothermal method have a simple preparation method and low cost. The water-sensitive carbon dots can be well dispersed in ethylene glycol and applied to detect the water content in ethylene glycol, thus having high practical value and good economic benefits.

The present invention is in the field of materials engineering and nanotechnology. More specifically, the present invention discloses: a functional premix comprising nanoparticles and a carrier or compatibilizer; a method of obtaining the premix; a method of altering the properties of a material; and a modified material formed with improved properties. Unexpectedly, the premix of the present invention can be used to alter the properties of different kinds of materials, such as polymers, metals, ceramic materials, and / or composites of different materials. The premix of the present invention has numerous advantages in terms of transportation, use, and incorporation of nanoparticles into different materials. In one embodiment, the present invention provides significant modification of the properties of polymers and, in fact, breaks the existing concepts in this technical field. In another embodiment, the present invention provides advantages in altering the properties of metals and / or ceramic materials.

The invention belongs to the technical fields of medical chemistry, new materials and nanotechnology, and relates to a novel diagnosis and treatment agent in the field of interdisciplinary, in particular to a preparation method of a reduction response activation < 19 > F magnetic resonance signal amplification self-degradation nanometer diagnosis and treatment micelle. Poly (benzyl carbamate) is obtained through condensation polymerization as a basic structure, then chemical modification is performed to obtain a self-degradable fluorine-containing amphiphilic block polymer, hydrophobic drug chlorin (Ce6) is loaded through self-assembly, the self-degradable fluorine-containing amphiphilic block polymer has the characteristic of responding to degradation of glutathione (GSH) at a tumor site, and on the basis of maintaining self-degradation of a hydrophobic chain segment of the nano-micelle, the self-degradable fluorine-containing amphiphilic block polymer can be used for preparing the self-degradable fluorine-containing amphiphilic block polymer. The nano-micelle loaded with Ce6 has the advantages that the nano-micelle loaded with Ce6 is used as a carrier, the characteristic of efficient 19F MR imaging is additionally endowed, under laser irradiation, the nano-micelle loaded with Ce6 can enhance a new scheme of breast cancer photodynamic therapy, the effects of responding to tumor microenvironment self-degradation drug release and 19F MRI signal amplification are achieved, and therefore diagnosis and treatment integration of breast cancer is achieved.

The invention discloses a molecular system based on DNA nano-structure artificial atoms and a construction and regulation method, and belongs to the technical field of DNA nanotechnology. The construction method of the molecular system comprises the following steps: mixing DNA chains with extension chains, adding a TM buffer solution, and carrying out annealing reaction to synthesize a DNA nano-structure with extension chains; dNA nanostructures are used for representing artificial atoms, extended chains on the DNA nanostructures are used for representing valence electrons, double chains formed by interaction among the extended chains are used for representing chemical bonds, a plurality of DNA nanostructures with extended chains are mixed in proportion according to a target molecular configuration, and a molecular system is obtained through one-pot assembly. Dynamic dissociation and recombination of artificial atoms are achieved based on DNA strand displacement reaction, and accurate regulation and control of a programmable molecular system are achieved. The construction precision and regulation effectiveness of the molecular system are verified through various technologies, the system has the advantages of being high in programmability, high in assembly efficiency, good in structural controllability and the like, and a brand new technical platform is provided for the fields of material design, chemical process simulation, biological engineering and the like.

This invention belongs to the field of biomedical materials and nanotechnology, specifically relating to the synthesis and application of a multifunctional mesoporous nanomaterial. The inventors synthesized a core-shell structured mesoporous nanoparticle using mesoporous silica nanoparticles and dopamine via emulsion epitaxy. The core of the core-shell structured mesoporous nanoparticle is S-nitrosoglutathione supported on mesoporous silica, and the outer shell is pentafluorophenyl chlorophyll supported on mesoporous polydopamine, forming MSN / G@mPDA / F nanoparticles. These nanoparticles rapidly increase in temperature to 45°C under 808 nm laser irradiation. o At temperatures above a certain temperature, S-nitrosoglutathione decomposes to produce nitric oxide. Further irradiation at 750 nm generates a large amount of reactive oxygen species, killing bacteria. This combination of photothermal and photodynamic therapy effectively inhibits bacterial growth. Therefore, this method exhibits excellent antibacterial effects and can play a role in the future treatment of bacterial infections.

The application discloses an intelligent response type DNA nano device and a construction method and application thereof, and belongs to the field of DNA nanotechnology; the intelligent response type DNA nano device comprises a plurality of different DNA tetrahedron basic units and extension chains and miRNA response chains arranged on the DNA tetrahedron basic units; wherein the DNA tetrahedron basic units are connected by base complementary pairing between the extension chains and the miRNA response chains to form a multimer structure; the miRNA response chain comprises a base sequence capable of specific complementary pairing with a target miRNA to be detected, and the extension chain and the miRNA response chain are separated, so that specific configuration transformation of the nano device is induced. Through programmable assembly of the DNA tetrahedron and miRNA-induced configuration dynamic response, high specificity, high sensitivity, multi-channel parallel detection of a plurality of miRNAs are realized, and problems such as insufficient specificity, low sensitivity and poor targeting of a traditional miRNA detection method are overcome, so that the application has a wide application prospect in the fields of biomedical research and clinical diagnosis.

The application discloses a kind of high stability two-dimensional nanometer material aerogel and preparation method thereof, it is related to material science and nanotechnology field.The water solution of two-dimensional nanometer material is frozen and handled to obtain frozen tissue, then foam structure is obtained by freeze drying, after which foam structure is soaked in ionic solution, freeze-dried, to obtain two-dimensional nanometer material aerogel.The application constructs two-dimensional nanometer material foam structure and introduces ionic bond crosslinking mechanism, so that two-dimensional nanometer material is combined by ionic bond to form high stability aerogel, so that aerogel has excellent performance.The application breaks through the selection limit of traditional aerogel material, and is suitable for ceramic two-dimensional material and MXenes with insufficient surface functional groups, lays a foundation for two-dimensional material aerogel large-scale production and application in the field of energy, aerospace and the like.

This invention discloses a method and system for determining the temperature / strain rate dependence of metal strength, belonging to the field of micro-nano technology. The method obtains the compression or tension stress-strain curves of micro-nano metal samples, as well as the background noise of the no-load test. Based on the background noise, the maximum displacement rate of the micro-nano mechanical testing instrument is determined and used as a threshold. Based on the threshold, the stress-strain curves are iterated to determine the start and end points of all dislocation avalanches, the total plastic displacement of the dislocation avalanches, and the intensity ratio of the micro-nano metal sample, thereby determining the temperature / strain rate dependence of the metal strength. This method, through testing a single sample at room temperature and calculating the intensity ratio, can quantitatively and accurately confirm whether the strength is temperature-dependent, thus avoiding the limitations of qualitative analysis and the need for multiple multi-sample experiments to save time, manpower, and other experimental costs.

FIELD: nanomaterials.SUBSTANCE: invention relates to chemical technology, nanotechnology and materials science and can be used in the production of highly efficient photocatalysts, materials for photoelectrochemical cells, sensors and functional coatings. First, laser ablation of the primary material, titanium nitride TiN, is carried out in a liquid, which is distilled or deionized water, resulting in the formation of a suspension of spherical TiN nanoparticles. The resulting nanoparticles are oxidized by heating the said suspension at 80-90 °C for 2-6 hours. Then centrifugation is performed for 20 minutes at a frequency of 15,000 rpm.EFFECT: obtaining nanocrystalline titanium dioxide flakes with a controlled content of the anatase phase of up to 90% and higher at atmospheric pressure in a neutral aqueous environment without the use of acids and morphological controlling agents, and also reducing the oxidation temperature.2 cl, 3 dwg

The present invention provides a method for detecting methylmercury chloride by using SERS technology, comprising the following steps: S1. Synthesis of gold sol; S2. Synthesis of probe molecule-functionalized gold nanoparticles; S3. Detection of methylmercury chloride by SERS technology. The present invention can detect the content of methylmercury chloride in water or food and can be applied in practice; the reaction can reach equilibrium within 3 minutes, and the reaction time is short; the linear range is 10-5 g / L to 10-1 g / L (10 ppb to 100 ppm), which is lower than the minimum standard of the world's authoritative institutions.

The application provides a kind of nanometer hole nuclear pore membrane rapid nondestructive testing method based on dark field scattering optical method, comprising: using dark field optical microscope to focus on the nuclear pore membrane to be detected, after focusing, adjust to dark field mode, obtain the scattering light image of the nuclear pore membrane to be detected under dark field mode;Determine the image area and the number of scattering light spots in the image based on the scattering light image, obtain the density of the nuclear pore membrane;Move the dark field optical microscope light spot center to the single hole position to collect dark field scattering spectrum, obtain the single hole dark field scattering spectrum intensity I ;Based on the relationship between scattering spectrum intensity I And aperture D Formula I = aD 2 +b , the aperture of the nuclear pore module is calculated by the single hole dark field scattering spectrum intensity I Therefore, the application has the advantages of simple operation, low cost, fast detection speed, etc., and can be widely applied in the field of nanotechnology and material science.

The invention discloses a targeted gating ratio type pH sensor of a double-CRISPR system based on tetrahedral framework nucleic acid, and belongs to the technical field of biomedicine and nucleic acid nanotechnology. According to the sensor, tetrahedral framework nucleic acid is used as a rigid support, Cas12a, Cas13a and corresponding fluorescent reporter molecules are accurately assembled according to the stoichiometric ratio of 1: 1, and orthogonal pH responses of the Cas12a and the Cas13a form a ratio type sensing basis; target recognition-pH sensing logic gating is realized through target specific crRNA (Complementary Ribonucleic Acid) design; after being modified by cholesterol, the compound can be stably anchored on the surface of a lipid membrane. The invention also discloses a preparation method of the sensor, a membrane anchoring method, a pH monitoring method and a target gating function realization method. The sensor has the advantages of high-precision ratio measurement, accurate spatial arrangement, high specificity, stable membrane anchoring, wide pH response range and the like, and can be used for accurately monitoring pH dynamics in a biological membrane interface microenvironment and extracellular vesicle fusion process.

The invention provides a preparation method of a composite carrier based on magnetic silicon-based nanoflowers, and relates to the technical field of nanotechnology and composite materials, and the preparation method comprises the following steps: S1, uniformly dispersing a reaction mixture containing a silicon source, magnetic iron oxide nanoparticles and a surface modifier in a solvent to obtain a reaction mixture, s2, the reaction mixture is treated under the hydrothermal reaction condition of 180-250 DEG C, the reaction time is 4-8 hours, the magnetic silicon-based nanoflower composite material is formed, the reaction temperature and time can promote magnetic iron oxide nanoparticles to be evenly distributed on the surface of a silicon-based carrier, and the magnetism and catalytic activity of the material are ensured. According to the preparation method of the composite carrier based on the magnetic silicon-based nanoflowers, the ionic liquid is adopted as a solvent and a surface functionalizing agent, so that the problems of aggregation, poor dispersity and the like of magnetic particles in a traditional method are effectively solved.

The invention relates to the field of nanoscience and nanotechnology, in particular to a particle thermodynamic measurement method and device based on a dual-laser confocal microscopy technology, in a solution, high-power continuous laser is adopted as capturing laser to capture particles, and movement of the particles in the capturing process is monitored in real time; the stress condition of the particles is analyzed according to the motion trail of the particles, then low-power continuous laser serves as Raman detection laser to be focused on the captured particles, Raman signals are excited, the temperature of the particles is quantified through the Raman spectrum technology, thermal measurement of the particles is conducted, and thermal measurement of the particles is achieved. The method provided by the invention can be used in the physical field to research the thermal-mechanical coupling problem in optical tweezers capture; the method can be applied to the field of biology, and accurate capture of cells or microorganisms is realized under the condition of low heat; the method can be applied to the field of energy utilization, and efficient utilization of energy is achieved by precisely regulating and controlling the thermodynamic property of the micro-nano structure.

The invention discloses a direct photoetching quantum dot patterning method and a QLED device prepared by the same, and belongs to the field of quantum dot (QD) nanotechnology, a hole transport material poly ((9, 9-dioctylfluorenyl-2, 7-diyl)-alt-(9-(2-ethynyl)-carbazole-3, 6-diyl)) (PF8Cz) and a photoinitiator benzophenone (BP) or bis-benzophenone (BPBP) are added into a toluene QD solution, so that the QD mixed solution has a photosensitive characteristic, selective exposure is realized, and the quantum dot is prepared by the method. According to the method, the solubility of the QD in an original solution is changed, and development is carried out, so that direct photoetching patterning of the QD is realized, a high-resolution and high-precision QD pattern is obtained, and a patterned QLED device with good performance is prepared. The main reaction mechanism is that after BP or BPBP is subjected to ultraviolet exposure, C = O in molecules is converted into active free radicals, and the active free radicals and alkyl branched chains in PF8Cz are subjected to cross-linking reaction to form a cross-linked network, so that QD in an exposure area is fixed, and a QD pattern is formed. The patterning mode does not need participation of quantum dot ligands, the risk that the quantum dot ligands fall off is reduced, and damage to the light emitting performance of the QD in the photoetching process can be reduced.

The invention discloses a USAG-1 recombinant protein targeted delivery system based on multi-enzyme logic gating and a preparation method, and mainly relates to crossing of biological medicine and nanotechnology. Comprising the following steps: S1, a C-terminal fusion nuclear localization sequence PKKKRKV of a recombinant USAG-1 protein expressed by CHO cells, and the purity of the recombinant USAG-1 protein is greater than or equal to 98%; s2, preparing core-shell structure nanoparticles, wherein the core is PLGA loaded BMP-2, and the drug loading capacity is 2.5-4.2 [mu] g / mg; the outer-layer lipid membrane is composed of DSPC / DSPG according to the ratio of 7: 3, and the surface embedded USAG-1 molecular density is greater than or equal to 1 * 10 / mu m < 2 >; s3, the surface co-modified EGFR antibody and the RGDfK peptide are connected through a thiol-maleimide bond and a PEG2000 spacer arm according to the molecular ratio of 1: 1.6 + / -0.2, the coding sequence of the recombinant USAG-1 is regulated and controlled by an MMP-9 response promoter, and the promoter is located in MMP-9gt; and the expression is activated at 20nM. The system has the beneficial effects that time-space precise delivery of the bone metabolism related protein USAG-1 is realized through a multi-enzyme logic gating mechanism, and the system is suitable for treatment of bone metabolism disorder diseases such as osteosarcoma and alveolar bone defect.

This invention relates to the field of biodetection technology, specifically to a multimodal miRNA detection array based on Mn-CeO2@CDs nanozymes and Cas12a sensing, its preparation method, and its applications. The recognition and signal amplification probe composition based on Mn-CeO2@CDs nanozymes and Cas12a includes: a solid substrate, the DNA Y-shaped structure described in the first aspect, the composite nanozyme, and a CRISPR / Cas12a system. This invention combines DNA nanotechnology, Cas12a enzyme digestion technology, and nanozymes as signal output mediators, achieving highly specific and sensitive multimodal detection of myocarditis biomarkers (miRNAs) in fluorescence, UV-visualization, and electrochemical modes.

The present application relates to the field of nanotechnology, in particular to a raffinose-SS31 peptide multi-targeted functional chitooligosaccharide / astaxanthin nanoparticle and a preparation method and application thereof.The nanoparticle of the present application takes precise regulation at the nanometer scale, innovative application of new components and targeted synergistic delivery as the core design idea, constructs a multifunctional nanofunctional network, provides support for the nanoscale precise delivery of active ingredients such as astaxanthin, and at the same time, establishes a chitooligosaccharide nanoparticle preparation technology with high active substance loading rate and stability for the problems of low stability and poor efficacy of chitooligosaccharide encapsulated active substances;targeting ligands are introduced by structure modification to enhance the specific recognition and binding capacity of nanoparticles to specific cells and tissues, and a targeted delivery technology for site-specific release and precise delivery of active substances is established.

The present invention provides a method and a measurement system for measuring three-dimensional atomic coordinates at the sub-nanometer scale. This method can achieve three-dimensional measurements with atomic precision, providing a more accurate measurement method and system for the development of nanotechnology. The invention uses the periodic atomic lattice arrangement pattern of lattice materials as a measurement scale. Its main components include: an atomic lattice measurement scale composed of a reference lattice material and its detection system, actuators for three axes, and a probe scanning module for detecting samples. Each nano-actuator on each axis is equipped with a counter. When the axis moves, the actuator drives the counter to count the atomic lattice, and then the result is used to feedback the movement distance of the axis. This system provides precise three-dimensional measurement functions for special application requirements from the sub-nanometer scale to the nanometer scale, achieving macroscopic measurement results with atomic lattice precision.

The invention discloses an extracellular vesicle biomarker detection method based on anion liposome membrane fusion and enzyme-free amplification, and belongs to the technical field of biomedicine nanotechnology. According to the method, multi-index direct detection of biomarkers (including micro ribonucleic acid and microRNA) in extracellular vesicles (EVs) can be realized by a one-step method through the anionic liposome, a complicated pretreatment process is not needed, and the method can be used for noninvasive early detection, real-time monitoring and therapeutic response evaluation. By optimizing the preparation method and detection conditions of the anion liposome, the optimized detection method is finally obtained, the complex process of extracellular vesicle extraction and internal microRNA release is avoided, the loss and caused errors in the process are reduced, and the detection sensitivity and accuracy are improved. The detection method provided by the invention can realize multi-index parallel detection, has high accuracy, can meet clinical application requirements, and can provide a new thought and technical scheme for detection of extracellular vesicle biomarkers of diseases such as tumors.

The invention discloses a porous polydopamine confinement MOF nanosphere and a preparation method and application thereof, and belongs to the field of material chemistry and nanotechnology, and the preparation method comprises the following steps: (1) synthesizing a mesoporous PDA-F127 nanosphere; (2) mixing the nanospheres obtained in the step (1) with an aluminum salt and an organic ligand in a solvent, and synthesizing an MOF-PDA-F127 composite material through a hydrothermal reaction; and (3) performing high-temperature carbonization treatment on the product in the step (2) to obtain the porous polydopamine confinement MOF nanospheres. The MOF-PDA-F127 nanosphere composite structure obtained by the invention has the characteristics of MOF size confinement, uniform distribution, excellent conductivity, good cycling stability and the like.

The present application relates to the field of nanoscience and nanotechnology, and particularly relates to a particle thermodynamic measurement method and device based on double laser confocal microscopy, in which a high-power continuous laser is used as a trapping laser to trap particles in a solution, and the movement of the particles in the trapping process is monitored in real time, the force acting on the particles is analyzed according to the movement trajectory of the particles, then a low-power continuous laser is used as a Raman detection laser to focus on the trapped particles to excite Raman signals, and Raman spectroscopy is used to quantify the temperature of the particles to perform particle thermometric measurement and realize thermodynamic measurement of the particles. The method of the present application can be used in the field of physics to study the thermodynamic coupling problem in optical tweezers trapping, can be used in the field of biology to achieve precise trapping of cells or microorganisms under low heat, and can be used in the field of energy utilization to realize efficient utilization of energy by precisely regulating the thermodynamic properties of micro-nano structures.

The invention provides a probe system and method for detecting testicular germ cell tumor miRNA based on PER-driven Split G4 / ThT cascade, belongs to the technical field of biological analysis and detection, and utilizes the advantages of orientation, controllable assembly and easy function integration of a DNA nanotechnology to detect testicular germ cell tumor miRNA. A target miRNA-started PER isothermal nucleic acid amplification technology and a product-induced programmed spet-G4-ThT specific fluorescence lightening characteristic are innovatively utilized to construct an interlocking type DNA nano device capable of programmatically responding to the testicular germ cell tumor miRNA biomarker, and through specific recognition of a target and programmed unlocking started by a PER product, the testicular germ cell tumor miRNA biomarker can be identified, so that the testicular germ cell tumor miRNA biomarker can be identified, and the testicular germ cell tumor miRNA biomarker can be identified. And a spet-G4-ThT signal in the nano device is induced to be started in a cascade manner, so that high-sensitivity and high-specificity accurate detection on the miRNA marker in the testicular germ cell tumor is realized.

This invention discloses a microfluidic-based polypeptide-based helical fiber with circularly polarized luminescence and its preparation method, belonging to the field of nanotechnology. Utilizing multiple hydrogen bonding interactions between polypeptides and based on the solute conformational confinement and ordered solvent diffusion mechanism of a microfluidic laminar flow chip, this invention develops a polypeptide-based circularly polarized luminescent material with excellent luminescence performance. Circular dichroism spectroscopy and circular polarization spectroscopy tests show that the helical fiber material obtained by this invention exhibits mirror-symmetric signals. Compared with materials obtained by traditional solution stirring, the polypeptide-based helical fibers prepared in the microfluidic chip by this invention not only have a more uniform morphology but also a larger... g lum value( L -Peptide / TBI and D The luminescence asymmetry factor of -Peptide / TBI is ±1.9×10⁻⁶. ‑3 Furthermore, this method has a certain degree of universality and is applicable to the assembly of other macromolecules, including polymers, nucleic acids, and proteins.

The invention provides a rapid pathogen detection method based on a nanotechnology. The method comprises the following steps: S1, sample collection: collecting pathogens from a clinical sample, wherein the pathogens comprise blood, urine, respiratory secretions and the like; s2, preparation of a magnetic bead resuspension: mixing a to-be-detected sample with magnetic beads to obtain a suspension, incubating the suspension under the conditions that the oscillation speed is 100-160rpm and the temperature is 21-36 DEG C for 2-4 hours, then performing magnetic separation, slowly resuspending the magnetic beads subjected to magnetic separation in a corresponding buffer solution to obtain the magnetic bead resuspension, and detecting the magnetic bead resuspension by using a chromatographic column to obtain the magnetic bead resuspension. S3, preparation of a gel solution: dissolving a certain amount of cetyltrimethylammonium bromide in 500mL of deionized water as an electrolyte, the method combines magnetic bead enrichment and solid nanopore technologies, can rapidly and accurately detect pathogens on a large scale at low cost, has the characteristics of high efficiency, sensitivity and specificity, and is suitable for large-scale detection of pathogens. In addition, the concentration of bacteria in the enriched pathogens is improved, and the pathogens can be rapidly detected.