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A synthesis method for a dopamine-modified magnetic mesoporous silica material and applications thereof

A synthesis method, mesoporous silicon technology, applied in the field of nanomaterials and nanometers, can solve problems affecting measurement, achieve low cost, excellent environmental stability and biocompatibility, and simplify experimental operations

Inactive Publication Date: 2013-08-07
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of biological samples, the interference of large molecular weight proteins seriously affects the determination of low molecular weight proteins in biological samples

Method used

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  • A synthesis method for a dopamine-modified magnetic mesoporous silica material and applications thereof
  • A synthesis method for a dopamine-modified magnetic mesoporous silica material and applications thereof
  • A synthesis method for a dopamine-modified magnetic mesoporous silica material and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The synthesis steps of magnetic medium silicon materials are as follows:

[0036] (1) Solubbing 1.35g hexal water chloride in 75ml ethylene glycol and stir until to clarify;

[0037] (2) Add sodium 3.6 g in the system and stir until it is clarified for 1 hour;

[0038] (3) After transferring the system ultrasound for 5 minutes, transfer to the reactor, react for 16 hours under 200 degrees Celsius;

[0039] (4) After washing the product for 5 times, the obtained product was used to obtain a four -iron magnetic micro -ball.

[0040] Dopamine modified magnetic medium silicon material synthesis method and fixing enzyme

[0041] (1) Divide the 10mg magnetic medium silicon material to a 10ml dopamine aqueous solution with a concentration of 1.0mm / L, and the ultrasonic 30 minutes to disperse it;

[0042] (2) Add a 30ml concentration of 0.2m (pH to 7.4) to the system of sodium phosphate-sodium-hydrogen-phosphate di-sodium tracting solution.

[0043] (3) Wash the obtained product th...

Embodiment 2

[0050] The synthesis of magnetic medium silicon materials, the specific steps are as follows:

[0051] (1) Solubbing 1.3g of hexasitic iron chloride in 70ml ethylene glycol and stir until clarify;

[0052] (2) Add 3.4 g of sodium acetate in the system and stir until it is clarified for 1 hour;

[0053](3) After transferring the system ultrasound for 5 minutes, transfer to the reactor, react for 16 hours under 200 degrees Celsius;

[0054] (4) After washing the product for 5 times, the obtained product was used to obtain a four -iron magnetic micro -ball.

[0055] The steps of the synthesis and fixing enzymes modified by dopamine modified magnetic medium silicon materials are as follows:

[0056] (1) Divide the 10mg magnetic medium silicon material to a 10ml dopamine aqueous solution with a concentration of 0.5mm / L, and the ultrasonic 30 minutes to disperse it;

[0057] (2) Add a 30ml concentration of 0.15m of sodium-hydrogen-phosphate-a hydrogen phosphate di-sodium buffer solution...

Embodiment 3

[0064] The synthesis of magnetic medium silicon materials, the specific steps are as follows:

[0065] (1) Solubbing 1.4g of hexal water chloride in 80ml of ethylene glycol and stir until to clarify;

[0066] (2) Add sodium 3.8g of sodium acetate in the system and stir until it is clarified for 3 hours;

[0067] (3) After transferring the system ultrasound for 5 minutes, transfer to the reactor, react for 16 hours under 200 degrees Celsius;

[0068] (4) After washing the product for 5 times, the obtained product was used to obtain a four -iron magnetic micro -ball.

[0069] The synthesis and fixing enzymes of dopamine modified magnetic medium silicon materials are as follows:

[0070] (1) Divide the 10mg magnetic medium silicon material to a 10ml dopamine aqueous solution with a concentration of 1.5mm / L, and the ultrasonic 30 minutes to disperse it;

[0071] (2) Add a 30ml concentration of 0.25m of sodium-hydrogen phosphate-a hydrogen phosphate di-sodium tracting solution with a c...

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Abstract

The invention belongs to the field of nanotechnology, and in particular to a synthesis method for a dopamine-modified magnetic mesoporous silica material and applications thereof. The synthesis method comprises: first, dispersing a magnetic mesoporous silica material in a dopamine solution uniformly; adding a sodium monohydrogen phosphate - disodium hydrogen phosphate buffer solution with a concentration of 0.2M, adding glutaraldehyde, and shaking the obtained solution for reaction at a room temperature for 0.5-1 hours; washing completely; dispersing the obtained product in above step in a sodium monohydrogen phosphate - disodium hydrogen phosphate buffer solution with a concentration of 0.2M, and shaking the obtained solution for reaction at the room temperature for 2-4 hours; washing completely; dispersing the obtained product in above step in a 2mg / mL trypsin-containing ammonium bicarbonate buffer solution with a concentration of 25mM, and shaking for reaction at the room temperature for 2 hours; and washing completely to remove surface impurities the product to give the desired material. According to the material, magnetic microspheres are employed as skeletons to provide magnetism to benefit subsequent separation and analysis, the pore size distribution and biocompatibility are good, the synthesis method is simple and low in cost, and size-exclusion selective enzymolysis can be performed by fixing enzyme inside the pore.

Description

Technical field [0001] The invention is a field of nanomaterials and nano -technology, which involves a synthesis method and application of magnetic medium silicon materials modified by dopamine modified for volume resistance selective enzymes. Background technique [0002] In recent years, protein group studies have become one of the fastest development of biological research and an indispensable part of life science research.If you want to obtain a detailed structure information of the protein, you often choose to crack the protein into a smaller polypeptide fragment through chemical hydrolysis or enzyme digestion, and then the obtained polypeptide mixture is analyzed into the mass spectrometry.The qualitative identification of peptide fingerprint graphics (PMF) is still the most powerful and simplest method in the current research.At present, the protein of small molecules has proven to be an important research object to understand the pathological application of biomarkers an...

Claims

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Application Information

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IPC IPC(8): C12N11/14G01N1/28
Inventor 邓春晖卜静
Owner FUDAN UNIV
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