Method for detecting nitrite ions on basis of polymer carbon dot fluorescence colorimetry

A nitrite ion and carbon dot fluorescence technology, applied in the nanometer field, can solve the problems of low detection sensitivity, cumbersome synthesis route, single output signal, etc., and achieve the effect of good sensitivity and simple synthesis process

Active Publication Date: 2018-09-28
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have some disadvantages, such as cumbersome synthetic routes, relatively single output signal, harsh reaction conditions, and

Method used

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  • Method for detecting nitrite ions on basis of polymer carbon dot fluorescence colorimetry
  • Method for detecting nitrite ions on basis of polymer carbon dot fluorescence colorimetry
  • Method for detecting nitrite ions on basis of polymer carbon dot fluorescence colorimetry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1: 5 mg of dopamine hydrochloride was weighed and dissolved in 10 mL of water, and 10 mg of o-phenylenediamine and 500 μL of concentrated hydrochloric acid were added. (The molar ratio of dopamine hydrochloride to o-phenylenediamine is 1:4) Transfer the mixture to a reaction kettle (20mL) at a reaction temperature of 200°C and heat in an oven for 8h to obtain blue polymer carbon dots with a concentration of 10mg / mL . Use Tris-HCl (20mM, pH=7) buffer solution to prepare 0.4mg / mL carbon dot solution for UV-visible spectrophotometer test, record its absorption spectrum; use Tris-HCl (20mM, pH=7) buffer solution to prepare 0.2mg / mL carbon dot solution was tested by fluorescence spectrophotometer, and its emission spectrum was recorded. The test obtained absorption wavelength is 380nm, 560nm, 610nm; emission wavelength is 625nm (Ex=580nm), respectively as figure 1 and figure 2 .

Embodiment 2

[0078] Example 2: 10 mg of dopamine hydrochloride was weighed and dissolved in 10 mL of water, and 10 mg of o-phenylenediamine and 500 μL of concentrated hydrochloric acid were added. (The molar ratio of dopamine hydrochloride to o-phenylenediamine is 1:2) The mixture was transferred to a reaction kettle (20mL) at a reaction temperature of 200°C and heated in an oven for 8h to obtain a blue solution with a concentration of 10mg / mL. Prepare 0.4mg / mL carbon dot solution with Tris-HCl (20mM, pH=7) buffer solution and carry out UV-visible spectrophotometer test, record its absorption spectrum; Tris-HCl (20mM, pH=7) buffer solution prepares 0.2mg / mL mL carbon dot solution was tested by fluorescence spectrophotometer, and its emission spectrum was recorded. The test obtained absorption wavelength is 380nm, 560nm, 610nm; emission wavelength is 625nm (Ex=580nm), respectively as image 3 and Figure 4 .

Embodiment 3

[0079] Example 3: 20 mg of dopamine hydrochloride was weighed and dissolved in 10 mL of water, and 10 mg of o-phenylenediamine and 500 μL of concentrated hydrochloric acid were added. (The molar ratio of dopamine hydrochloride to o-phenylenediamine is 1:1) The mixture was transferred to a reaction kettle (20mL) at a reaction temperature of 200°C and heated in an oven for 8h to obtain a blue solution with a concentration of 10mg / mL. Use Tris-HCl (20mM, pH=7) buffer solution to prepare 0.4mg / mL carbon dot solution for UV-visible spectrophotometer test, record its absorption spectrum; use Tris-HCl (20mM, pH=7) buffer solution to prepare 0.2mg / mL carbon dot solution was tested by fluorescence spectrophotometer, and its emission spectrum was recorded. The test obtained absorption wavelength is 380nm, 560nm, 610nm; emission wavelength is 625nm (Ex=580nm), respectively as Figure 5 and Figure 6 .

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Abstract

The invention belongs to the technical field of nanometer, and discloses preparation of polymer carbon dots and a detection method thereof applied to nitrite ion colorimetry fluorescence. When different concentrations of nitrite ions exist in a buffer system containing a certain amount of polymer carbo dots, for an absorption spectrum, the absorption peak in a 610nm position is obviously reduced;the absorption peak in a 380 nm position is gradually increased; the intensity of the fluorescence emission peak in a 625 nm position of the fluorescence spectrum is gradually reduced along with the increasing of the nitrite ion; Therefore according to the change of polymer carbon dot fluorescence spectrum and absorption spectrum in a system, through fluorescence colorimetry multi-output signal analysis, the high-sensitivity and high-selectivity detection on the nitrite ions can be realized; the detection on the nitrite ions in a complicated system such as urine can be further realized. A polymer carbon dot-based fluorescent colorimetric probe is a novel expansion on the original analysis method, and is hopeful to be used for the analysis and detection of nitrite ions in the complicated systems such as food, environment and the like.

Description

technical field [0001] The invention relates to a method for detecting nitrite ions based on polymer carbon dot fluorescence colorimetry and an application thereof, belonging to the field of nanotechnology. Background technique [0002] Nitrite is a chemical substance that needs to be strictly controlled by national, industrial and local standards. As a preservative and additive, it has been widely used in food, natural water sources, environment and service industries. However, nitrite is a serious hazard to the environment and human health. On the one hand, excessive nitrite in the human body can lead to irreversible oxidation of hemoglobin and reduce blood volume; on the other hand, nitrite interacts with amines and transforms into carcinogenic nitrosamines, which are potentially toxic, mutagenic and carcinogenic sex. In addition, nitrite can also cause enterogenous cyanosis, teratogenicity and other hazards. Therefore, it is necessary to develop a method for the highl...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 刘金华赵朵朵陈灿李林张承武于海东
Owner NANJING UNIV OF TECH
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