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This invention belongs to the field of contact lens technology, and provides a moisturizing protein-based contact lens and its preparation method. The preparation method includes the following steps: mixing a silk fibroin solution and a hyaluronic acid-binding protein solution to obtain a protein mixture solution; mixing a hydrophilic modification component, an oxygen-permeable modification component, and water to obtain a composite modifier; mixing the protein mixture solution, the composite modifier, and a crosslinking agent to obtain a pre-crosslinked mixture; molding the pre-crosslinked mixture and performing gradient crosslinking, followed by sequential soaking in buffer solution, rinsing, and sterilization to obtain the moisturizing protein-based contact lens. The contact lens of this invention uses protein as the main component, does not rely on silicon-based substances or harmful crosslinking agents, and possesses high biocompatibility, high oxygen permeability, suitable mechanical properties, and controllable degradation.