The invention discloses ultraviolet (UV) glass protective printing ink and a manufacturing method thereof. The printing ink comprises, by weight, 25-50 parts of urethane acrylate resin, 20-45 parts of acrylate crylic acid resin, 1-10 parts of hydroxyethyl methylacrylate, 1-10 parts of 1,6-ethylene acrylic acid ester, 1-8 parts of dipentaerythritol hexaacrylate, 6-12 parts of photoinitiators, 3-18 parts of toner, 1-4 parts of gas-phase silica, 2-5 parts of antifoaming agents, 2-5 parts of dispersing agents, and 1-3 parts of silane attaching accelerant. The components are added in sequence according to parts thereof, stirring is carried out at constant speed for full time, then grinding is carried out through a three-roller machine, and finally the printing ink can be obtained by filtering a solution. In a glass machining process of a touch screen, silk screen is carried out on the surface of glass for protection with the UV glass protective printing ink, final perfectness rate of glass products is improved by 27%-40% compared with the situation when a glass protective measurement is not taken, resource waste can be obviously reduced, enterprise manufacturing cost is lowered, and enterprise production capacity is improved.