70 results about "Serous fluid" patented technology

The invention relates to the technical field of biological extraction, and provides a cattle spleen peptide composition as well as a preparation method and application thereof. The preparation method of the cattle spleen peptide comprises the following steps: S1, taking a cattle spleen, adding normal saline, and homogenizing to obtain first homogenate; s2, adding combined enzyme into the first homogenate, uniformly stirring, and performing first water bath treatment to obtain second homogenate; s3, adding chymotrypsin into the second homogenate, uniformly stirring, and performing second water bath treatment to obtain third homogenate; s4, centrifuging the third homogenate to obtain supernate; s5, performing ultrafiltration and drying on the supernate to obtain the cattle spleen peptide. The combined enzyme comprises subtilisin, bromelain and papain. Through the technical scheme, the problem of low cattle spleen peptide extraction rate in related technologies is solved.

The invention discloses a preparation method of elastin peptide, and relates to the technical field of natural elastin extraction.The preparation method comprises the following steps that S1, animal blood vessels are cleaned, subjected to impurity removal, cut into blocks and minced; s2, the diced and minced animal blood vessels are soaked in alkali liquor for degreasing, the animal blood vessels are fished out and cleaned after degreasing, water is added after cleaning, pulping is conducted, slurry is obtained, and the pH of the slurry is adjusted to 7.5-8.5; s3, adding elastinase and trypsin into the pulp, and performing enzymolysis to obtain enzymatic hydrolysate; s4, sequentially performing high-temperature enzyme deactivation, vacuum concentration, purification, refining and spray drying on the enzymatic hydrolysate to obtain powdery elastin peptide. The trypsin and elastinase adopted in the invention belong to basic proteins, can decompose proteins under alkaline conditions, and have the characteristics of high catalysis efficiency, action specificity, activity adjustability, diversity and the like.

The invention provides a preparation method of a terminal-peptide-free bovine type I collagen product. The preparation method comprises the following steps: obtaining a crushed bovine tissue raw material without impurities; the preparation method comprises the following steps: soaking a cattle tissue raw material in acid, and homogenizing to obtain cattle tissue homogenate; protease is added into the bovine tissue homogenate in batches for enzymolysis, the enzymolysis temperature is controlled to be 2-6 DEG C, after salting-out precipitation, pH value adjustment and redissolution are carried out on the bovine type I collagen extracting solution obtained in the middle batch, protease is added again for enzymolysis, salting-out precipitation, pH value adjustment and redissolution are carried out again, and the bovine type I collagen extracting solution is obtained. Repeating for multiple times to obtain a terminal-peptide-free bovine type I collagen solution; adjusting the pH value of the terminal-peptide-free bovine type I collagen solution, salting out, precipitating, centrifuging and washing to obtain terminal-peptide-free bovine type I collagen precipitate; and purifying the terminal-peptide-free bovine type I collagen precipitate to obtain the terminal-peptide-free bovine type I collagen product. According to the telopeptide-free bovine type I collagen product provided by the invention, no residual telopeptide is detected, and the purity is high.

The invention discloses a slurry permeation consolidation rate test method, test system and computer equipment, and the test method comprises the following steps: S1, pouring standard sand into a cylindrical test device, leveling the surface of the standard sand in the cylindrical test device to be horizontal, and measuring the height H of the standard sand in the cylindrical test device; s2, taking slurry with the volume of # imgabs0 #, and slowly pouring the slurry into the cylindrical testing device; s3, the cylindrical testing device is placed in the steel sleeve, an air bag is used for blocking an opening of the cylindrical testing device, the interior of the cylindrical testing device is pressurized through a pressurizing device, the mixture of the standard sand and the slurry is pressurized to p, the pressure maintaining time is t, and the slurry can fully permeate into the standard sand; and S4, removing the pressurizing device, measuring the overall height h of a mixture of the slurry and the standard sand after complete permeation, and calculating a pressure permeation consolidation rate index # imgabs 1 #. The pressure permeation consolidation rate index is adopted as an evaluation index, and accurate quantitative evaluation of the permeation and solidification performance of the slurry in the stratum can be achieved.

The invention discloses a chickpea sprout peptide. The amino acid sequence of the chickpea sprout peptide is as follows: Glu-Glu-Cys-Pro-Cys-Ala-Asn-Cys-Cys, wherein the amino acid sequence of the chickpea sprout peptide is as follows: Glu-Glu-Cys-Pro-Cys-Ala-Asn-Cys-Cys; the preparation method of the chickpea sprouting peptide comprises the following steps: step 1, sprouting chickpeas until the sprouting length is 0.5-1.5 cm, wherein the sprouting time is 12-72 hours; step 2, mixing and pulping germinated chickpeas and water according to a weight ratio of 1: 1-1: 1.2, and adjusting the pH value of the pulp to 6.5-8.0; step 3, adding neutral protease and compound flavor protease, and performing enzymolysis at the enzymolysis temperature of 50-55 DEG C for 2-3 hours; step 4, treating the enzymatic hydrolysate through an ultrafiltration membrane with the molecular weight cut-off of 5000Da under the conditions that the working pressure is 0.15-0.25 MPa and the working temperature is 20-45 DEG C, and collecting filtrate; and step 5, carrying out corrosion prevention and drying on the filtrate to obtain the chickpea sprout peptide. The chickpea sprout peptide has the effects of resisting obesity, resisting oxidation and adjusting immunity, in addition, the preparation method can achieve efficient and high-quality preparation of the chickpea sprout peptide, the chickpea sprout peptide is accurately screened, and the ineffective experiment cost is reduced.

The invention belongs to the field of biological material extraction, and particularly relates to a method for extracting collagen peptide from marine organisms, which comprises the following steps: S1, crushing marine organism raw materials, soaking the crushed marine organism raw materials in a citric acid buffer solution containing alginate oligosaccharide for swelling treatment, and then performing high-voltage pulse electric field treatment to obtain pretreated collagen slurry; and S2, carrying out first-stage enzymolysis treatment on the pretreated collagen slurry through alkaline protease, and then carrying out second-stage enzymolysis treatment through collagenase and flavourzyme. S3, performing separation treatment through a first ultrafiltration membrane to obtain first filtrate, performing deodorization treatment on the first filtrate through a first nanofiltration membrane of which the surface is coated with a beta-cyclodextrin adsorbent to obtain second filtrate, and performing separation treatment on the second filtrate through a second nanofiltration membrane to obtain third filtrate. And S4, concentrating, desalting and drying the third filtrate to obtain collagen peptide powder. Through the synergistic effect of multiple steps, the concentration of the molecular weight of the collagen peptide and the efficient deodorization are realized on the basis of retaining the active structure of the collagen.

The invention belongs to the field of traditional Chinese medicine preparations, and particularly relates to an easy-to-absorb oral liquid capable of promoting qi-blood tonifying and a preparation process thereof. Comprising the following steps: preparing an absorption promoter; preparing a stable compatilizer; steaming and extracting the traditional Chinese medicinal materials; and preparing the oral liquid. The method comprises the following steps: carrying out enzymolysis on mixed slurry prepared from asparagus flowers and resina liquidamberis by utilizing cellulose-pectin compound enzyme, freeze-drying the prepared resina liquidamberis-asparagus flower enzymatic hydrolysate to obtain an absorption enhancer, and adding the absorption enhancer in the process of preparing the oral liquid, the absorption rate of intestinal tracts to active ingredients of Chinese wolfberry fruits, poria cocos, Chinese dates and longan aril can be increased, and the effect of promoting qi and blood tonifying and easy absorption of the oral liquid on blood and qi tonifying of human bodies is improved.

The invention discloses a collagen injection for tendon repair as well as a preparation method and application thereof, and belongs to the technical field of collagen preparation. The preparation method disclosed by the invention comprises the following steps: by taking a collagen dry product as a raw material, sequentially dissolving, carrying out secondary filtration, separating, preparing fibrotic collagen slurry A, preparing gelatinized collagen slurry B, preparing the solution, homogenizing and filling to prepare the collagen injection capable of being used for tendon repair. The collagen injection provided by the invention shows multiphase transformation during refrigeration and contact with body fluid with body temperature, and does not have the phenomenon of fiber layering in the long-term storage process. The collagen disclosed by the invention is an injection type gel preparation, can be flexibly injected and applied according to the depth and the area of a wound, is beneficial to promoting tissue regeneration and functional repair, and is particularly suitable for tendon injury and other scenes needing collagen to guide cell repair.

The invention relates to the technical field of modernization of traditional Chinese medicines, in particular to a nano-vesicle, a preparation method and application of the nano-vesicle in preparation of an endometrial injury repairing medicine. The preparation method comprises the following steps: taking a periplaneta americana ethanol extracting solution, and adding a buffer solution to prepare diluted homogenate; performing differential centrifugal treatment on the homogenate; filtering the supernatant obtained by the differential centrifugation treatment, and then centrifuging for 60-120 minutes by 100000g of the supernatant to obtain a precipitate; and re-suspending the precipitate to obtain the periplaneta americana ethanol extract source nano-vesicles. The periplaneta americana ethanol extract is used as a starting material to enrich the nano-vesicles for the first time, the combined process of differential centrifugation, membrane filtration and ultracentrifugation is adopted, the obtained nano-vesicles can be applied to the field of endometrial injury repair, especially treatment of gynecological diseases such as intrauterine adhesion and infertility with thin intima, and the blank in the technical field is filled.

Provided in the present disclosure are materials, devices, systems, and methods for performing cryo-surgery in a mammalian subject. More particularly, presented are a slurry, devices, systems, and methods for subcutaneously injecting the slurry into a patient in need of bodyfat reduction intervention. The slurry, devices, systems, and methods permit highly efficacious subcutaneous ice phase-change lipolysis while not requiring that the patient be placed under general anesthesia, nor requiring significant new surgical training among practitioners.

Provided is a technology for improving the efficiency of separating a complex of a protein to be purified and a carrier in a hydrocyclone of a device including an in-line mixer and the hydrocyclone. A protein purification device disclosed herein comprises a first supply unit, a second supply unit, an in-line mixer, a hydrocyclone, and a dilution line. The first supply unit supplies a first slurry containing a carrier including a ligand that binds to a protein. The second supply unit supplies a second slurry which is a cell culture solution containing a protein. The in-line mixer mixes the first slurry and the second slurry. The hydrocyclone separates, downstream of the in-line mixer, a complex of the protein and the carrier from a mixed slurry of the first slurry and the second slurry. The dilution line is provided between the in-line mixer and the hydrocyclone and introduces a diluted liquid into the mixed slurry.

The invention relates to the field of agricultural production, in particular to a plant nutrition preparation and a preparation method thereof. The preparation method of the plant nutrition preparation comprises the following steps: (a) pulping a mixed solution containing sea cucumber visceral tissues and a thiol compound to obtain slurry; (b) adding protease into the slurry, performing enzymolysis, filtering and diluting to obtain a micromolecular collagen peptide solution; and (c) adding nutrient elements into the heated micromolecular collagen peptide solution, and then carrying out chelation reaction. The preparation method of the plant nutrition preparation has the advantages of being environmentally friendly, easy to operate, low in cost and the like, the obtained plant nutrition preparation can provide various nutrient substances for plant growth and promote plant growth, and reasonable utilization of waste sea cucumber resources is achieved.

The invention relates to the technical field of geological reinforcement, and discloses a grout stopping method for stratum pre-grouting reinforcement, which adopts an A and B double-component grout system: the B component contains thermal stability urease inhibited by a reversible inhibitor; the component A contains a corresponding inhibitor and a corresponding scavenger, and the two components are mixed to release the reaction through inhibition, so that the controllable prolonging of the slurry gelation time is realized, and the blockage of a pipeline is avoided. A self-indication and slow-release carrier loaded with copper ions is arranged in the component A, after the slurry reaction is started, ammonium ions of a reaction product replace the copper ions, the blue change of the slurry is caused, and a visual basis is provided for judging the injection stopping opportunity. The component A further comprises an energy field response medium and reserve urea packaged in the carrier, and the energy field response medium and the reserve urea enable the consolidation body to have the later non-contact remote control enhancement capacity and the micro-crack passive self-repairing capacity respectively. The grouting process is safe and controllable, the reaction state is visible in real time, and the performance of the consolidated body is regulated and controlled in the later period.

The present disclosure provides novel compounds, compositions, and methods for modulating one or more septin proteins, e.g., septin-2. Such compounds and compositions are useful for the treatment of a cancer, e.g., endometrial, pancreatic, lung, breast or ovarian cancer, such as serous and ovarian clear cell carcinoma.

A guided mode resonance (GMR) sensor that can be used to simultaneously detect an array of analytes. It provides a diagnostic system that can rapidly detect an array of biomarker proteins in patient samples (such as blood, serum or plasma for example) which can be used as an accurate means to conduct a differential analysis of proteins that allows the discrimination of early and late stages of disease, such as metastatic versus primary ovarian serous carcinomas. The GMR sensor can be provided in a compact size such that it can be portable.

Cryogranulation systems with improved dispenser assemblies are provided for use in manufacturing frozen pellets of pharmaceutical substances in a fluid medium. Methods of cryogranulating the pharmaceutical substance in the fluid medium are also provided. In particular embodiments, the dispenser assembly is used with suspensions or slurries of pharmaceutical compositions including biodegradable substances, such as proteins, peptides, and nucleic acids. In certain embodiments, the pharmaceutical substance can be adsorbed to any pharmaceutically acceptable carrier particles suitable for making pharmaceutical powders. In one embodiment, the pharmaceutical carrier can be, for example, diketopiperazine-based microparticles. The dispenser assembly improves the physical characteristics of the cryopellets formed and minimizes product loss during processing.

The invention provides a subretinal effusion region segmentation and volume calculation method and system, a product and a terminal, and the method comprises the steps: automatically segmenting a fundus prediction lesion region image based on a pre-constructed fundus lesion region prediction model, and carrying out the summation of pixel points, belonging to a subretinal effusion part, in the data of each fundus prediction lesion region image. According to the method, the volume data of the subretinal fluid is obtained, the film reading time of a fundus disease doctor is greatly shortened, the working efficiency is improved, in addition, the film reading accuracy, stability and homogeneity can be remarkably improved, and clinical doctors can be helped to accurately and objectively evaluate the central serous chorioretinopathy.

The application discloses a bird's nest compound product with the effect of improving female body health and a preparation method and application thereof. The preparation method comprises the following steps: mixing red ginseng, Gastrodia elata, ophiopogon, donkey-hide gelatin, eucommia and ganoderma lucidum with a solvent to form a mixed slurry; treating and extracting the mixed slurry by applying an electric field to prepare a compound liquid; and mixing the compound liquid with bird's nest to prepare the bird's nest compound product with the effect of improving female body health. The bird's nest is matched with the medicine-food homologous material, and on the basis of the theory of "suo zhuo jun chen", the bird's nest compound product capable of obviously improving menopausal syndrome is prepared, the hormone level is effectively adjusted, symptoms such as hot flashes, night sweats, sleep quality improvement, ovary and uterus repair promotion and the like are relieved, and the product is mild in action, high in safety, good in flavor and color.

The present application relates to the field of traditional Chinese medicine, in particular to a kind of traditional Chinese medicine composition for treating central serous chorioretinopathy and application thereof.The present application provides a kind of traditional Chinese medicine composition for treating central serous chorioretinopathy, compared with the prescription of Chunzai Decoction, the traditional Chinese medicine composition of the present application increases the efficacy of promoting blood circulation and removing blood stasis, soothing the liver and relieving depression, clearing the liver and improving eyesight, clearing heat and reducing phlegm on the basis of Chunzai Decoction warming yang, transforming qi, reducing water and removing dampness;Compared with using Chunzai Decoction alone, the curative effect of patients using the traditional Chinese medicine composition is more direct, from 2-3 weeks to 1 week, and the curative effect is accelerated.And the traditional Chinese medicine composition of the present application has been used for about 2 years, 2 / 3 of the 112 patients can see effect in 1 week, and no recurrence is found within 3 months.

The present invention provides a method of treating high-grade serous cancer in a patient in need thereof, comprising administering to the patient in need thereof an effective amount of an SIK2 / 3 inhibitor or a pharmaceutically acceptable salt thereof, where the effective amount is lower than the amount required to directly kill tumor cells. The method of treating high-grade serous cancer in immune-functioning patients in need thereof may also include administering an immune checkpoint inhibitor.

The application discloses a kind of livestock product drug residue detection methods based on near infrared spectroscopy technology, it is related to trace detection field, the detection method includes the following steps: S1, livestock product sample is mixed with buffer solution, and obtain homogenate liquid;S2, hydroxylamine hydrochloride solution is added to homogenate liquid, and oscillation reaction is obtained under mild conditions, and reaction liquid is obtained;S3, nanometer gold stick dispersion liquid is added to reaction liquid, and mixed liquid is obtained;S4, near infrared spectrum of mixed liquid is collected;S5, pretreatment is carried out, and local surface plasmon resonance peak position is extracted, and peak position offset is calculated;S6, peak position offset is substituted into quantitative model, and drug residue concentration is calculated.The application realizes the in-situ release of total residue by selective rupture of the covalent bond between drug and protein, and realizes the high-sensitivity detection of sulfonamides, tetracyclines and fluoroquinolones under the condition of extraction-free by combining the signal amplification effect of nanometer gold stick.

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus gasseri KB24 and application thereof in preparation of cattle spleen peptide for improving spleen deficiency. The preparation method comprises the following steps: (1) pretreatment: removing envelopes of the cattle spleen, crushing, and homogenizing to prepare cattle spleen slurry; (2) enzymolysis treatment: adding a compound enzyme into the cattle spleen slurry for enzymolysis, and sterilizing after enzymolysis to obtain an enzymatic hydrolysate; (3) performing fermentation treatment: inoculating lactobacillus gasseri KB24 into the enzymatic hydrolysate, and performing fermentation to obtain fermentation liquor; and (4) carrying out solid-liquid separation, filtration, ultrafiltration membrane separation and freeze drying on the fermentation liquor to obtain the cattle spleen peptide. The cattle spleen peptide prepared by the method can improve the spleen deficiency symptom from the aspects of weight, holding power, exhaustive swimming time, gastrointestinal digestive function, energy substance metabolism and the like, and can be applied to products for improving spleen deficiency.

The application provides a blood-activating and stasis-resolving external use plaster and a preparation process thereof, and the plaster is a microneedle-plaster composite preparation which is composed of a drug active ingredient, a sustained-release agent, a penetrating agent, an absorbent and a humectant. The drug active ingredient includes various Chinese herbal medicines such as Dysosma Versipellis, frankincense and myrrh, and the absorbent is a mixture of polyglutamic acid and snail polypeptide solution. The preparation process includes the steps of crushing medicinal materials, extracting with ethanol, concentrating and collecting cream, preparing a mixed solution, preparing a microneedle-plaster composite preparation and coating and the like. The snail polypeptide solution is obtained by enzymolysis of snail homogenate, and the humectant can be selected from hesperidin, glycerol and the like. The dissolvable material is selected from polylactic acid-hydroxyacetic acid copolymer and the like, and the ultrasonic power and frequency and the stirring speed of ultrasonic stirring all have certain ranges. The plaster has the effects of activating blood and resolving stasis and strengthening muscles and bones, and is suitable for external use. The preparation process is simple and feasible, and has high practical value and application prospect.

The invention discloses a prefabricated residence laminated slab strip slurry leakage prevention structure and a construction method, the prefabricated residence laminated slab strip slurry leakage prevention structure comprises a strip body, and the strip body is arranged between adjacent laminated slab bodies; the prefabricated cushion block is arranged at the connecting part of the slab strip body and the laminated slab body; the sealing mechanism is arranged at the joint of the prefabricated cushion block and the laminated slab body; the reinforced sealing assembly is arranged on the outer side of the sealing mechanism and used for providing dynamic sealing and mechanical blocking during concrete pouring; the internal dredging assembly is pre-arranged in the prefabricated cushion block and is used for collecting and discharging slurry which can permeate into the prefabricated cushion block; the formwork fastening assembly is arranged on the two sides of the plate strip body in a striding mode and used for fixing a pouring formwork and ensuring the forming quality of a plate strip area; the internal dredging assembly and the formwork fastening assembly with the synergistic effect are creatively additionally arranged, the formwork fastening assembly achieves tight attachment of the pouring formwork and the laminated slab through straddling fixation, and sealing failure caused by supporting deformation is reduced.

The invention belongs to the technical field of biological peptide processing, and particularly discloses oyster peptide for improving male functions and a preparation method of the oyster peptide. The preparation method comprises the following steps: S1, mixing fresh oyster meat with an algae substance, adding deionized water, grinding, adjusting the pH value to 6.5-7.0, and carrying out ultrasonic treatment to obtain a homogenate; s2, adding compound protease into the homogenate obtained in the step S1, and performing enzymolysis to obtain enzymatic hydrolysate; s3, adding an organic matter into the enzymatic hydrolysate obtained in S2, and heating for reaction to obtain a reaction solution; s4, adjusting the pH value of the reactant obtained in S3 to 5.5-6.5, adding lactic acid bacteria, and fermenting to obtain fermentation liquor; and S5, performing inactivation and ultrafiltration on the fermentation liquor obtained in the step S4 to obtain the oyster peptide. The invention discloses an oyster peptide capable of improving male functions and a preparation method thereof. The oyster peptide obtained by adopting the preparation method is high in yield, free of fishy smell and capable of remarkably enhancing male function activity.

The invention discloses a method for detecting the concentration of flurbiprofen in pig tissue, and belongs to the technical field of biological medicine detection. The method provided by the invention comprises the following steps: cutting a pig tissue sample treated by flurbiprofen plaster into pieces, adding a PBS (Phosphate Buffer Solution), and grinding by using a biological homogenizer to obtain homogenate; performing vortex treatment on the homogenate to obtain vortex homogenate, then mixing the vortex homogenate with an internal standard working solution, performing vortex treatment and centrifugation to obtain a supernatant, then adding a formic acid aqueous solution, performing vortex uniform mixing, and then performing analysis by using liquid chromatography-tandem mass spectrometry. The method provided by the invention has the advantages of good repeatability, wide linear range, high sensitivity, high recovery rate, simple operation and efficient analysis.

Systems and methods for non-invasive leukocyte counting in serous bodily fluids. The system (100) includes () an ultrasound device (110) that generates ultrasound images (102) from a body (104) or a sample (106) in a scanning direction (x) at a configurable spatial resolution, and an image processing unit (120) that receives first ultrasound images (412) generated by the ultrasound device (110) at a first spatial resolution, and, for each first ultrasound image (412), generates a second ultrasound image (412) at a second spatial resolution, the method further includes determining a region (422) corresponding to the serous body fluid, determining a target focal point (432) in the region (422), and receiving at least one second ultrasound image (442) generated by the ultrasound device (110) focused on the target focal point (432) in the scanning direction (x) at a second spatial resolution higher than the first spatial resolution. The image processing unit (120) predicts the concentration (130) of leukocytes from the at least one second ultrasound image (442) using an artificial neural network (2100) trained with images having assigned reference truth value concentrations.

The invention relates to the technical field of peptide extraction, in particular to sipunculus nudus peptide and an extraction method and application thereof. According to the specific technical scheme, the method comprises the following steps: 1) crushing a sipunculus nudus raw material, adding water, adding sodium hydrogen sulfite and ascorbic acid, and stirring and soaking for 10-15 minutes; 2) grinding the soaked raw materials, and adjusting the pH value of the slurry to be alkaline; (3) adding alkali enzyme into the slurry, carrying out microwave enzymolysis for 45-50 minutes, adding papaya peptidase B, continuously carrying out microwave enzymolysis for 45-50 minutes, heating, inactivating, cooling, carrying out centrifugal filtration, and collecting filtrate; and 4) sequentially carrying out microfiltration, ultrafiltration and sodium filtration on the obtained filtrate to obtain the product. Microwave radiation is adopted for assisting enzymolysis, energy can be provided for an enzymolysis system, the temperature needed by enzymolysis can be maintained, the enzymolysis efficiency is greatly improved, the prepared sipunculus nudus peptide can stabilize iron ions, a safe and efficient iron supplementing preparation can be developed, meanwhile, reproductive function decline caused by obesity can be comprehensively repaired, the advantage of multi-dimensional conditioning is achieved, and the sipunculus nudus peptide has a good application prospect. And the method is suitable for metabolism-related reproductive disorder treatment.

The invention provides an experimental device for enhancing removal of trichloroethylene by in-situ biological sulfurized zero-valent iron. The experimental device comprises a sample injection bag, a peristaltic pump, a seepage column and a post-treatment column, a stainless steel dispensing needle head is inserted into the sample injection bag, the outer end of the dispensing needle head is connected with a Luer internal rotary joint, and the Luer internal rotary joint is connected with a first polytetrafluoroethylene hard tube through a first hose; the right end of the peristaltic pump is connected with the sample injection bag; the upper end of the seepage column is connected with the post-treatment column; the lower end is connected with the peristaltic pump; openings are formed in the side wall of 30 cm in the middle of the seepage column every 5 cm, and the openings are connected through three-way connectors; the middle of the seepage column is divided into six layers to be filled with quartz sand, domestication bacterium liquid is evenly injected into the surface of each layer of quartz sand, and xanthan gum modified zero-valent iron slurry is injected into the seepage column from the side face and the bottom of the seepage column in a multi-point circulation mode. According to the technical scheme, the system error of trichloroethylene removal by the experimental device is reduced, the true degradation phase of a filling medium to trichloroethylene is reduced, the problems of pre-vulcanization and experimental dechlorination separation are solved, the in-situ biological vulcanization and dechlorination effects are improved, and the remediation target of'simultaneous vulcanization and dechlorination 'is achieved.

The invention belongs to the technical field of antarctic krill peptide preparation, and discloses a preparation method of antarctic krill peptide with antioxidant activity, which comprises the following steps: unfreezing antarctic krill, adding water, homogenizing to obtain slurry, adding a modified nano silicon dioxide agent into the slurry, and stirring to obtain nano silicon dioxide modified slurry liquid; carrying out enzymolysis treatment on the nano silicon dioxide modified pulp liquid, after the treatment is finished, taking supernate, and filtering to obtain a product I; and 2, fermenting residues obtained after taking the supernate in the step 2, after the treatment is finished, taking the supernate, concentrating and drying to obtain a product 2, merging the product 1 and the product 2, and performing spray drying to obtain the euphausia superba peptide with antioxidant activity. When the euphausia superba peptide is prepared, the yield is high, the removal rate of hydroxyl radicals and superoxide anions of the product is high, and the antioxidant activity of the product is excellent.