33 results about "Serous fluid" patented technology

The invention provides a preparation method of a terminal-peptide-free bovine type I collagen product. The preparation method comprises the following steps: obtaining a crushed bovine tissue raw material without impurities; the preparation method comprises the following steps: soaking a cattle tissue raw material in acid, and homogenizing to obtain cattle tissue homogenate; protease is added into the bovine tissue homogenate in batches for enzymolysis, the enzymolysis temperature is controlled to be 2-6 DEG C, after salting-out precipitation, pH value adjustment and redissolution are carried out on the bovine type I collagen extracting solution obtained in the middle batch, protease is added again for enzymolysis, salting-out precipitation, pH value adjustment and redissolution are carried out again, and the bovine type I collagen extracting solution is obtained. Repeating for multiple times to obtain a terminal-peptide-free bovine type I collagen solution; adjusting the pH value of the terminal-peptide-free bovine type I collagen solution, salting out, precipitating, centrifuging and washing to obtain terminal-peptide-free bovine type I collagen precipitate; and purifying the terminal-peptide-free bovine type I collagen precipitate to obtain the terminal-peptide-free bovine type I collagen product. According to the telopeptide-free bovine type I collagen product provided by the invention, no residual telopeptide is detected, and the purity is high.

The invention belongs to the field of traditional Chinese medicine preparations, and particularly relates to an easy-to-absorb oral liquid capable of promoting qi-blood tonifying and a preparation process thereof. Comprising the following steps: preparing an absorption promoter; preparing a stable compatilizer; steaming and extracting the traditional Chinese medicinal materials; and preparing the oral liquid. The method comprises the following steps: carrying out enzymolysis on mixed slurry prepared from asparagus flowers and resina liquidamberis by utilizing cellulose-pectin compound enzyme, freeze-drying the prepared resina liquidamberis-asparagus flower enzymatic hydrolysate to obtain an absorption enhancer, and adding the absorption enhancer in the process of preparing the oral liquid, the absorption rate of intestinal tracts to active ingredients of Chinese wolfberry fruits, poria cocos, Chinese dates and longan aril can be increased, and the effect of promoting qi and blood tonifying and easy absorption of the oral liquid on blood and qi tonifying of human bodies is improved.

The invention discloses a collagen injection for tendon repair as well as a preparation method and application thereof, and belongs to the technical field of collagen preparation. The preparation method disclosed by the invention comprises the following steps: by taking a collagen dry product as a raw material, sequentially dissolving, carrying out secondary filtration, separating, preparing fibrotic collagen slurry A, preparing gelatinized collagen slurry B, preparing the solution, homogenizing and filling to prepare the collagen injection capable of being used for tendon repair. The collagen injection provided by the invention shows multiphase transformation during refrigeration and contact with body fluid with body temperature, and does not have the phenomenon of fiber layering in the long-term storage process. The collagen disclosed by the invention is an injection type gel preparation, can be flexibly injected and applied according to the depth and the area of a wound, is beneficial to promoting tissue regeneration and functional repair, and is particularly suitable for tendon injury and other scenes needing collagen to guide cell repair.

The invention relates to the technical field of modernization of traditional Chinese medicines, in particular to a nano-vesicle, a preparation method and application of the nano-vesicle in preparation of an endometrial injury repairing medicine. The preparation method comprises the following steps: taking a periplaneta americana ethanol extracting solution, and adding a buffer solution to prepare diluted homogenate; performing differential centrifugal treatment on the homogenate; filtering the supernatant obtained by the differential centrifugation treatment, and then centrifuging for 60-120 minutes by 100000g of the supernatant to obtain a precipitate; and re-suspending the precipitate to obtain the periplaneta americana ethanol extract source nano-vesicles. The periplaneta americana ethanol extract is used as a starting material to enrich the nano-vesicles for the first time, the combined process of differential centrifugation, membrane filtration and ultracentrifugation is adopted, the obtained nano-vesicles can be applied to the field of endometrial injury repair, especially treatment of gynecological diseases such as intrauterine adhesion and infertility with thin intima, and the blank in the technical field is filled.

Provided in the present disclosure are materials, devices, systems, and methods for performing cryo-surgery in a mammalian subject. More particularly, presented are a slurry, devices, systems, and methods for subcutaneously injecting the slurry into a patient in need of bodyfat reduction intervention. The slurry, devices, systems, and methods permit highly efficacious subcutaneous ice phase-change lipolysis while not requiring that the patient be placed under general anesthesia, nor requiring significant new surgical training among practitioners.

Provided is a technology for improving the efficiency of separating a complex of a protein to be purified and a carrier in a hydrocyclone of a device including an in-line mixer and the hydrocyclone. A protein purification device disclosed herein comprises a first supply unit, a second supply unit, an in-line mixer, a hydrocyclone, and a dilution line. The first supply unit supplies a first slurry containing a carrier including a ligand that binds to a protein. The second supply unit supplies a second slurry which is a cell culture solution containing a protein. The in-line mixer mixes the first slurry and the second slurry. The hydrocyclone separates, downstream of the in-line mixer, a complex of the protein and the carrier from a mixed slurry of the first slurry and the second slurry. The dilution line is provided between the in-line mixer and the hydrocyclone and introduces a diluted liquid into the mixed slurry.

The invention relates to the technical field of geological reinforcement, and discloses a grout stopping method for stratum pre-grouting reinforcement, which adopts an A and B double-component grout system: the B component contains thermal stability urease inhibited by a reversible inhibitor; the component A contains a corresponding inhibitor and a corresponding scavenger, and the two components are mixed to release the reaction through inhibition, so that the controllable prolonging of the slurry gelation time is realized, and the blockage of a pipeline is avoided. A self-indication and slow-release carrier loaded with copper ions is arranged in the component A, after the slurry reaction is started, ammonium ions of a reaction product replace the copper ions, the blue change of the slurry is caused, and a visual basis is provided for judging the injection stopping opportunity. The component A further comprises an energy field response medium and reserve urea packaged in the carrier, and the energy field response medium and the reserve urea enable the consolidation body to have the later non-contact remote control enhancement capacity and the micro-crack passive self-repairing capacity respectively. The grouting process is safe and controllable, the reaction state is visible in real time, and the performance of the consolidated body is regulated and controlled in the later period.

The application discloses a bird's nest compound product with the effect of improving female body health and a preparation method and application thereof. The preparation method comprises the following steps: mixing red ginseng, Gastrodia elata, ophiopogon, donkey-hide gelatin, eucommia and ganoderma lucidum with a solvent to form a mixed slurry; treating and extracting the mixed slurry by applying an electric field to prepare a compound liquid; and mixing the compound liquid with bird's nest to prepare the bird's nest compound product with the effect of improving female body health. The bird's nest is matched with the medicine-food homologous material, and on the basis of the theory of "suo zhuo jun chen", the bird's nest compound product capable of obviously improving menopausal syndrome is prepared, the hormone level is effectively adjusted, symptoms such as hot flashes, night sweats, sleep quality improvement, ovary and uterus repair promotion and the like are relieved, and the product is mild in action, high in safety, good in flavor and color.

The present application relates to the field of traditional Chinese medicine, in particular to a kind of traditional Chinese medicine composition for treating central serous chorioretinopathy and application thereof.The present application provides a kind of traditional Chinese medicine composition for treating central serous chorioretinopathy, compared with the prescription of Chunzai Decoction, the traditional Chinese medicine composition of the present application increases the efficacy of promoting blood circulation and removing blood stasis, soothing the liver and relieving depression, clearing the liver and improving eyesight, clearing heat and reducing phlegm on the basis of Chunzai Decoction warming yang, transforming qi, reducing water and removing dampness;Compared with using Chunzai Decoction alone, the curative effect of patients using the traditional Chinese medicine composition is more direct, from 2-3 weeks to 1 week, and the curative effect is accelerated.And the traditional Chinese medicine composition of the present application has been used for about 2 years, 2 / 3 of the 112 patients can see effect in 1 week, and no recurrence is found within 3 months.

The present invention provides a method of treating high-grade serous cancer in a patient in need thereof, comprising administering to the patient in need thereof an effective amount of an SIK2 / 3 inhibitor or a pharmaceutically acceptable salt thereof, where the effective amount is lower than the amount required to directly kill tumor cells. The method of treating high-grade serous cancer in immune-functioning patients in need thereof may also include administering an immune checkpoint inhibitor.

The application discloses a kind of livestock product drug residue detection methods based on near infrared spectroscopy technology, it is related to trace detection field, the detection method includes the following steps: S1, livestock product sample is mixed with buffer solution, and obtain homogenate liquid;S2, hydroxylamine hydrochloride solution is added to homogenate liquid, and oscillation reaction is obtained under mild conditions, and reaction liquid is obtained;S3, nanometer gold stick dispersion liquid is added to reaction liquid, and mixed liquid is obtained;S4, near infrared spectrum of mixed liquid is collected;S5, pretreatment is carried out, and local surface plasmon resonance peak position is extracted, and peak position offset is calculated;S6, peak position offset is substituted into quantitative model, and drug residue concentration is calculated.The application realizes the in-situ release of total residue by selective rupture of the covalent bond between drug and protein, and realizes the high-sensitivity detection of sulfonamides, tetracyclines and fluoroquinolones under the condition of extraction-free by combining the signal amplification effect of nanometer gold stick.

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus gasseri KB24 and application thereof in preparation of cattle spleen peptide for improving spleen deficiency. The preparation method comprises the following steps: (1) pretreatment: removing envelopes of the cattle spleen, crushing, and homogenizing to prepare cattle spleen slurry; (2) enzymolysis treatment: adding a compound enzyme into the cattle spleen slurry for enzymolysis, and sterilizing after enzymolysis to obtain an enzymatic hydrolysate; (3) performing fermentation treatment: inoculating lactobacillus gasseri KB24 into the enzymatic hydrolysate, and performing fermentation to obtain fermentation liquor; and (4) carrying out solid-liquid separation, filtration, ultrafiltration membrane separation and freeze drying on the fermentation liquor to obtain the cattle spleen peptide. The cattle spleen peptide prepared by the method can improve the spleen deficiency symptom from the aspects of weight, holding power, exhaustive swimming time, gastrointestinal digestive function, energy substance metabolism and the like, and can be applied to products for improving spleen deficiency.

The invention discloses a prefabricated residence laminated slab strip slurry leakage prevention structure and a construction method, the prefabricated residence laminated slab strip slurry leakage prevention structure comprises a strip body, and the strip body is arranged between adjacent laminated slab bodies; the prefabricated cushion block is arranged at the connecting part of the slab strip body and the laminated slab body; the sealing mechanism is arranged at the joint of the prefabricated cushion block and the laminated slab body; the reinforced sealing assembly is arranged on the outer side of the sealing mechanism and used for providing dynamic sealing and mechanical blocking during concrete pouring; the internal dredging assembly is pre-arranged in the prefabricated cushion block and is used for collecting and discharging slurry which can permeate into the prefabricated cushion block; the formwork fastening assembly is arranged on the two sides of the plate strip body in a striding mode and used for fixing a pouring formwork and ensuring the forming quality of a plate strip area; the internal dredging assembly and the formwork fastening assembly with the synergistic effect are creatively additionally arranged, the formwork fastening assembly achieves tight attachment of the pouring formwork and the laminated slab through straddling fixation, and sealing failure caused by supporting deformation is reduced.

The invention belongs to the technical field of biological peptide processing, and particularly discloses oyster peptide for improving male functions and a preparation method of the oyster peptide. The preparation method comprises the following steps: S1, mixing fresh oyster meat with an algae substance, adding deionized water, grinding, adjusting the pH value to 6.5-7.0, and carrying out ultrasonic treatment to obtain a homogenate; s2, adding compound protease into the homogenate obtained in the step S1, and performing enzymolysis to obtain enzymatic hydrolysate; s3, adding an organic matter into the enzymatic hydrolysate obtained in S2, and heating for reaction to obtain a reaction solution; s4, adjusting the pH value of the reactant obtained in S3 to 5.5-6.5, adding lactic acid bacteria, and fermenting to obtain fermentation liquor; and S5, performing inactivation and ultrafiltration on the fermentation liquor obtained in the step S4 to obtain the oyster peptide. The invention discloses an oyster peptide capable of improving male functions and a preparation method thereof. The oyster peptide obtained by adopting the preparation method is high in yield, free of fishy smell and capable of remarkably enhancing male function activity.

The invention relates to the technical field of peptide extraction, in particular to sipunculus nudus peptide and an extraction method and application thereof. According to the specific technical scheme, the method comprises the following steps: 1) crushing a sipunculus nudus raw material, adding water, adding sodium hydrogen sulfite and ascorbic acid, and stirring and soaking for 10-15 minutes; 2) grinding the soaked raw materials, and adjusting the pH value of the slurry to be alkaline; (3) adding alkali enzyme into the slurry, carrying out microwave enzymolysis for 45-50 minutes, adding papaya peptidase B, continuously carrying out microwave enzymolysis for 45-50 minutes, heating, inactivating, cooling, carrying out centrifugal filtration, and collecting filtrate; and 4) sequentially carrying out microfiltration, ultrafiltration and sodium filtration on the obtained filtrate to obtain the product. Microwave radiation is adopted for assisting enzymolysis, energy can be provided for an enzymolysis system, the temperature needed by enzymolysis can be maintained, the enzymolysis efficiency is greatly improved, the prepared sipunculus nudus peptide can stabilize iron ions, a safe and efficient iron supplementing preparation can be developed, meanwhile, reproductive function decline caused by obesity can be comprehensively repaired, the advantage of multi-dimensional conditioning is achieved, and the sipunculus nudus peptide has a good application prospect. And the method is suitable for metabolism-related reproductive disorder treatment.

The invention relates to the technical field of peptide extraction, in particular to phascolosoma esculenta peptide and an extraction method and application thereof. According to the specific technical scheme, the method comprises the following steps: 1) crushing phascolosoma esculenta raw materials, adding water, adding sodium hydrogen sulfite and ascorbic acid, and stirring and soaking for 10-15 minutes; 2) grinding the soaked raw materials, and adjusting the pH value of the slurry to be alkaline; (3) adding alkali enzyme into the slurry, carrying out microwave enzymolysis for 45-50 minutes, adding papaya peptidase B, continuously carrying out microwave enzymolysis for 45-50 minutes, heating, inactivating, cooling, carrying out centrifugal filtration, and collecting filtrate; and 4) sequentially carrying out microfiltration, ultrafiltration and sodium filtration on the obtained filtrate to obtain the product. According to the phascolosoma esculenta peptide and the preparation method thereof, microwave radiation is adopted for assisting enzymolysis, energy can be provided for an enzymolysis system, the temperature needed by enzymolysis can be maintained, the enzymolysis efficiency is greatly improved, the prepared phascolosoma esculenta peptide can stabilize iron ions, a safe and efficient iron supplementing preparation can be developed, meanwhile, reproductive function decline caused by obesity can be comprehensively repaired, the advantage of multi-dimensional conditioning is achieved, and the phascolosoma esculenta peptide has a good application prospect. And the method is suitable for metabolism-related reproductive disorder treatment.

The application relates to the field of biomedical cosmetology, and provides an injection filling material and a preparation and application thereof. The preparation method of the injection filling material is that slurry formed by decellularized allogenic dermal microparticles with D50 of 100-155 mu m is mixed with an aqueous solution of sodium hyaluronate with a concentration of 1-1.5%, and then irradiation is carried out under the condition of 5-25 kGy for 10-30 min; the slurry is obtained by jointly crushing decellularized allogenic dermis and purified water; the injection filling material of the application has a wide application range, the injection filling material of the application not only makes up for the defect that the degradation time of pure sodium hyaluronate is short, but also makes up for the defect that the decellularized allogenic dermal filling material is prone to delamination by utilizing the chemical bonding reaction of sodium hyaluronate and dermal microparticles after mixing and irradiation, so that the stability of the system is increased, and the injection filling material is more suitable for medical and cosmetic filling materials.

The application belongs to the field of traditional Chinese medicine preparations, and particularly relates to an oral liquid capable of promoting easy absorption of qi and blood tonification and a preparation process thereof. The preparation process comprises the following steps: preparation of an absorption promoting agent, preparation of a stabilizing and compatible agent, steaming and extraction of traditional Chinese medicinal materials, and preparation of the oral liquid. The absorption promoting agent is prepared by enzymolysis of mixed slurry of Adiantum capillus-veneris flowers and liquidambar resin using cellulose-pectin complex enzyme, and the absorption promoting agent is obtained by freeze-drying of the prepared liquidambar resin-Adiantum capillus-veneris enzymolysis liquid. The absorption promoting agent is added in the preparation process of the oral liquid, so that the absorption rate of effective components of Lycium chinense, Poria cocos, jujube and longan aril in the intestinal tract is improved, and the effect of the oral liquid capable of promoting easy absorption of qi and blood tonification on blood and qi tonification of human bodies is improved.

The present application relates to the field of medicine, and discloses a polygonatum exosome, a preparation method thereof and application of the polygonatum exosome in preparation of a medicine for treating kidney fibrosis diseases, the preparation method comprising the following steps: grinding polygonatum rhizome and a phosphate buffer to prepare a slurry, performing first centrifugation on the obtained slurry to remove solid impurities, obtaining supernatant, performing second centrifugation on the supernatant to obtain a precipitate, resuspending the precipitate with the phosphate buffer, and obtaining the polygonatum exosome.

The application discloses a kind of development and application of marine protein source alcoholism-preventing liver-protecting product, belong to biotechnology field.Product preparation includes the following steps: (1) to marine organism homogenate liquid first protease is added to carry out enzymolysis, inactivation after enzymolysis is completed, obtain primary enzymolysis liquid;Second protease is added to primary enzymolysis liquid to carry out enzymolysis, inactivation after enzymolysis is completed, obtain secondary enzymolysis liquid.(2) the supernatant of secondary enzymolysis liquid is obtained by centrifugation.(3) the supernatant is concentrated, freeze-drying to obtain target powder.The application uses 8 kinds of marine organisms as raw materials, adopts 7 kinds of protease, and obtains 336 samples by double-enzyme stepwise enzymolysis;Based on the stability of gastrointestinal digestion, amino acid composition and molecular weight distribution, the raw material is determined as sea cucumber, and the alcoholism-preventing liver-protecting product with high stability, high hydrophobic amino acid composition and low molecular weight is obtained by stepwise hydrolysis using alkaline protease and flavour protease.The application is suitable for food health care field.

The invention provides linseed oil for purifying heart and cerebral vessels, a preparation method, a vegetable oil product and application, and relates to the technical field of food. The preparation method comprises the following steps: (1) carrying out crushing, segmented heating, steaming and frying and pulping treatment on flaxseeds to obtain flaxseed pulp; (2) extracting the flaxseed slurry with water, ethanol and normal hexane in sequence to obtain an extraction mixture A; (3) mixing and extracting the extraction mixture A and a deep eutectic solvent, and taking an upper layer to obtain an extraction mixture B; the eutectic solvent is a mixture of choline chloride and n-butyl alcohol; and (4) extracting the mixture B under reduced pressure to remove the solvent, and carrying out post-treatment to obtain the linseed oil. According to the preparation method, the yield of the linseed oil can be increased, the acid value and the peroxide value are reduced, and the linseed oil has excellent effects of reducing blood pressure, reducing blood fat, maintaining the healthy level of blood pressure and maintaining the healthy level of blood fat, and can be applied to preparation of vegetable oil with the effect of purifying heart and cerebral vessels.

The application discloses a pharmaceutical-grade PDRN and a preparation method thereof, and belongs to the technical field of biotechnology. The method comprises the following steps: (1) grinding salmon testis tissue in a pre-cooled grinding buffer to obtain a homogenate; (2) using Forskolin to induce the grinding homogenate; the induction conditions are as follows: first, adding Forskolin to 8-12 muM, and standing for 1-3 hours at 4 DEG C; then, increasing the temperature to 10-20 DEG C, adding Forskolin to 18-22 muM and 2 mM glutathione, and treating for 2 hours; and (3) extracting the PDRN through SDS-sodium chloride.

The invention discloses an umbilical cord extract as well as a preparation method and application thereof, and belongs to the technical field of animal umbilical cord extraction. The method comprises the following steps: firstly, adding digestive enzyme mixed liquor into animal umbilical cord homogenate, and then sequentially carrying out pulse ultrasonic treatment and constant-temperature oscillation incubation; secondly, carrying out step-by-step pressurizing and homogenizing treatment on the solution obtained in the previous step; and finally, repeating the previous step for 2-3 cycles, centrifugally collecting supernate, and sequentially carrying out step-by-step gradient aperture filtration and concentration filtration on the supernate to obtain the umbilical cord extract. Therefore, the method is an efficient and safe preparation method of the umbilical cord extract, effective components in the umbilical cord can be rapidly and efficiently extracted, and meanwhile, the activity and the yield of the product are guaranteed.

The invention discloses a cubilose compound product with an effect of improving female body health as well as a preparation method and application of the cubilose compound product. The preparation method comprises the following steps: mixing the red ginseng, the gastrodia elata, the radix ophiopogonis, the donkey-hide gelatin, the eucommia ulmoides and the lucid ganoderma with the solvent to form mixed slurry; applying an electric field to the mixed slurry for treatment and extraction to prepare a compound solution; and mixing the compound liquid with cubilose to prepare the cubilose compound product with the effect of improving the health of female organisms. The cubilose compound product capable of obviously improving climacteric syndrome is prepared by compatibility of cubilose and medicinal and edible materials on the basis of assistant monarch and minister theory, can effectively regulate hormone level, relieve hectic fever and night sweat, improve sleep quality, promote ovary and uterus repair and other symptoms, and is mild in action, high in safety and free of toxic and side effects. The flavor and the color are good.

The invention discloses a polypeptide obtained by a fermentation method and application thereof, and belongs to the technical field of bioactive peptides. The amino acid sequences of the polypeptide obtained by a fermentation method are as shown in SEQ ID NO: 1-SEQ ID NO: 3. According to the method, oyster homogenate is fermented through bacillus subtilis, bioactive peptides with the blood sugar reducing effect are separated and identified from an oyster protein solution through separation and purification technologies such as ultrafiltration, gel chromatography and LC-MS / MS, screening is further conducted through a bioinformatics technology, and finally three peptide fragments are obtained. According to the present invention, the peptide fragment sequence is identified, chemical synthesis is performed according to the identified peptide fragment sequence, the blood sugar reducing effects of the synthesized single peptide fragment and the compounded peptide fragment are determined, and the peptide fragments have good alpha-glucosidase inhibition activity, and have important application prospects in the development of the products with the blood sugar reducing effect.

The utility model discloses a device for preparing crude heparin sodium from small intestinal mucosa, which comprises an integrated frame, a PLC (programmable logic controller) is mounted on one side of the integrated frame, and a primary enzymolysis tank and a secondary enzymolysis tank are respectively fixed at two ends inside the integrated frame. When the device runs, treated small intestine mucous membrane slurry is added into the primary enzymolysis tank through the corrosion-resistant pump on the feeding pipe fitting, and the external temperature control heating sleeves are fixed on the outer walls of the primary enzymolysis tank and the secondary enzymolysis tank; a water bath heating cavity which is provided with an electric heating wire and a temperature sensor and is arranged in the side wall of the external temperature control heating sleeve is matched, the temperature control heating effect on the primary enzymolysis tank and the secondary enzymolysis tank can be achieved, and then the timing control effect of the PLC is matched, so that after the temperature control timing reaction of the materials in the primary enzymolysis tank is achieved, the reaction time is shortened; and the material is automatically transferred into the secondary enzymolysis tank through a corrosion-resistant pump on the material transferring pipe fitting to complete secondary temperature control timing reaction, so that the device realizes continuous two-time enzymolysis treatment.

This invention discloses a multi-directional circulating grouting system for microbial reinforcement of weak interlayer structures, comprising: a grouting system including a grout supply unit, a delivery pipeline unit, and a peristaltic pump; a clamping system including a base, a lifting rod assembly, a rotating assembly, and a clamping assembly; a weak interlayer structure sample, the sample being installed inside a model box; a waste liquid recovery system installed on the top surface of the base and located at the bottom of the model box; a pressure detection unit installed on the delivery pipeline unit; and a control system for controlling the operation of the entire device. This invention, by setting a central injection hole and coordinating with the rotating grouting of the sample, enables the grout to penetrate into the center of the structural surface, significantly improving the seepage path and distribution uniformity of the grout in the weak interlayer.

The invention belongs to the technical field of natural product extraction, and relates to an improved method for extracting phycocyanin from spirulina. The method comprises the following steps: (1) adding water into spirulina, freezing and thawing once, and then carrying out ultrasonic treatment and homogenization once in sequence; (2) centrifuging the homogenate, taking a supernatant, adjusting the pH value to 4.0-4.5, stirring for a period of time, centrifuging, taking the supernatant, performing ultrafiltration for one or more times by using an ultrafiltration membrane, and taking a filtrate; by utilizing the improved method for extracting phycocyanin from spirulina, when phycocyanin is extracted from spirulina, the extraction amount of phycocyanin can be remarkably increased, the extraction time is shortened, and the extraction cost is reduced.

The application relates to the technical field of polypeptide extraction processes, in particular to a Phascolosoma esculenta active peptide, a preparation method thereof and a chewable tablet. The preparation method of the Phascolosoma esculenta active peptide comprises the following steps: crushing and treating washed Phascolosoma esculenta in water to obtain Phascolosoma esculenta slurry; performing first enzymolysis treatment on the Phascolosoma esculenta slurry by using neutral protease to obtain first enzymolysis liquid; performing first enzyme inactivation treatment on the first enzymolysis liquid, then adding flavor protease to perform second enzymolysis treatment to obtain second enzymolysis liquid; performing second enzyme inactivation treatment on the second enzymolysis liquid, then performing centrifugal separation to obtain supernatant; and performing filtration treatment on the supernatant to obtain Phascolosoma esculenta active peptide with a molecular weight of less than 10 kDa. The Phascolosoma esculenta active peptide obtained by the preparation method has better antioxidant activity, can relieve the oxidation stress of a body caused by D-galactose, and has a certain protective effect on liver injury caused by subacute aging.

Compositions, such as aqueous solutions and non-aqueous solutions and slurries, of a weak acid are provided. The disclosed compositions may be used to treat, prevent, and / or reduce one or more symptoms associated with numerous vaginal conditions or disturbances, such as odor and itch.