90 results about "Serous fluid" patented technology

The invention relates to the technical field of biological extraction, and provides a cattle spleen peptide composition as well as a preparation method and application thereof. The preparation method of the cattle spleen peptide comprises the following steps: S1, taking a cattle spleen, adding normal saline, and homogenizing to obtain first homogenate; s2, adding combined enzyme into the first homogenate, uniformly stirring, and performing first water bath treatment to obtain second homogenate; s3, adding chymotrypsin into the second homogenate, uniformly stirring, and performing second water bath treatment to obtain third homogenate; s4, centrifuging the third homogenate to obtain supernate; s5, performing ultrafiltration and drying on the supernate to obtain the cattle spleen peptide. The combined enzyme comprises subtilisin, bromelain and papain. Through the technical scheme, the problem of low cattle spleen peptide extraction rate in related technologies is solved.

The invention provides a method for simultaneously detecting perfluorinated compounds in mouse tissues and organs, and belongs to the technical field of analysis and detection. The method provided by the invention comprises the following steps: mixing a mouse visceral organ tissue sample, an alkaline buffer solution and an internal standard solution, and homogenizing to obtain homogenate; mixing the homogenate with tetrabutylammonium hydrogen sulfate and methyl tert-butyl ether, and carrying out liquid-liquid extraction to obtain extract liquor; and sequentially drying and redissolving the extract liquor, and carrying out ultra-high performance liquid chromatography-tandem mass spectrometry analysis to obtain a result of the perfluorinated compound in the mouse tissue visceral organs. The result of the embodiment shows that the adding standard recovery rate of 14 target objects in the method provided by the invention is 64.9%-120.3%, the RSD of 6 repeated tests is 1.0%-14.0%, the matrix effect of the target objects is 83.7%-134.0%, and the matrix effect is relatively low; and the detection result has excellent accuracy and reproducibility.

The invention discloses a preparation method of elastin peptide, and relates to the technical field of natural elastin extraction.The preparation method comprises the following steps that S1, animal blood vessels are cleaned, subjected to impurity removal, cut into blocks and minced; s2, the diced and minced animal blood vessels are soaked in alkali liquor for degreasing, the animal blood vessels are fished out and cleaned after degreasing, water is added after cleaning, pulping is conducted, slurry is obtained, and the pH of the slurry is adjusted to 7.5-8.5; s3, adding elastinase and trypsin into the pulp, and performing enzymolysis to obtain enzymatic hydrolysate; s4, sequentially performing high-temperature enzyme deactivation, vacuum concentration, purification, refining and spray drying on the enzymatic hydrolysate to obtain powdery elastin peptide. The trypsin and elastinase adopted in the invention belong to basic proteins, can decompose proteins under alkaline conditions, and have the characteristics of high catalysis efficiency, action specificity, activity adjustability, diversity and the like.

The present invention provides a method for preparing a keratinized gingival repair scaffold, wherein the active powder of biological microspheres is dispersed in a protein slurry to obtain a loose surface stock solution, which is freeze-dried together with a cell-free matrix material, and after two cross-linkings, a keratinized gingival repair scaffold with a loose surface and a dense surface is obtained. When in use, the loose surface of the porous sponge structure contacts the wound surface, which is conducive to the attachment and growth of tissue cells, and the dense surface can seal and protect the wound to a certain extent in an open healing environment. The obtained keratinized gingival repair scaffold can effectively maintain and regulate the pH environment in the oral cavity, inhibit bacteria and resist bacteria, and inhibit the growth of acid-producing bacteria such as Streptococcus mutans and bacteria with strong caries-causing properties, and at the same time has the ability to stop bleeding, promote bone growth and periodontal ligament regeneration, and inhibit periodontal epithelial regression.

The invention provides a preparation method of a terminal-peptide-free bovine type I collagen product. The preparation method comprises the following steps: obtaining a crushed bovine tissue raw material without impurities; the preparation method comprises the following steps: soaking a cattle tissue raw material in acid, and homogenizing to obtain cattle tissue homogenate; protease is added into the bovine tissue homogenate in batches for enzymolysis, the enzymolysis temperature is controlled to be 2-6 DEG C, after salting-out precipitation, pH value adjustment and redissolution are carried out on the bovine type I collagen extracting solution obtained in the middle batch, protease is added again for enzymolysis, salting-out precipitation, pH value adjustment and redissolution are carried out again, and the bovine type I collagen extracting solution is obtained. Repeating for multiple times to obtain a terminal-peptide-free bovine type I collagen solution; adjusting the pH value of the terminal-peptide-free bovine type I collagen solution, salting out, precipitating, centrifuging and washing to obtain terminal-peptide-free bovine type I collagen precipitate; and purifying the terminal-peptide-free bovine type I collagen precipitate to obtain the terminal-peptide-free bovine type I collagen product. According to the telopeptide-free bovine type I collagen product provided by the invention, no residual telopeptide is detected, and the purity is high.

The invention discloses a slurry permeation consolidation rate test method, test system and computer equipment, and the test method comprises the following steps: S1, pouring standard sand into a cylindrical test device, leveling the surface of the standard sand in the cylindrical test device to be horizontal, and measuring the height H of the standard sand in the cylindrical test device; s2, taking slurry with the volume of # imgabs0 #, and slowly pouring the slurry into the cylindrical testing device; s3, the cylindrical testing device is placed in the steel sleeve, an air bag is used for blocking an opening of the cylindrical testing device, the interior of the cylindrical testing device is pressurized through a pressurizing device, the mixture of the standard sand and the slurry is pressurized to p, the pressure maintaining time is t, and the slurry can fully permeate into the standard sand; and S4, removing the pressurizing device, measuring the overall height h of a mixture of the slurry and the standard sand after complete permeation, and calculating a pressure permeation consolidation rate index # imgabs 1 #. The pressure permeation consolidation rate index is adopted as an evaluation index, and accurate quantitative evaluation of the permeation and solidification performance of the slurry in the stratum can be achieved.

The invention discloses a chickpea sprout peptide. The amino acid sequence of the chickpea sprout peptide is as follows: Glu-Glu-Cys-Pro-Cys-Ala-Asn-Cys-Cys, wherein the amino acid sequence of the chickpea sprout peptide is as follows: Glu-Glu-Cys-Pro-Cys-Ala-Asn-Cys-Cys; the preparation method of the chickpea sprouting peptide comprises the following steps: step 1, sprouting chickpeas until the sprouting length is 0.5-1.5 cm, wherein the sprouting time is 12-72 hours; step 2, mixing and pulping germinated chickpeas and water according to a weight ratio of 1: 1-1: 1.2, and adjusting the pH value of the pulp to 6.5-8.0; step 3, adding neutral protease and compound flavor protease, and performing enzymolysis at the enzymolysis temperature of 50-55 DEG C for 2-3 hours; step 4, treating the enzymatic hydrolysate through an ultrafiltration membrane with the molecular weight cut-off of 5000Da under the conditions that the working pressure is 0.15-0.25 MPa and the working temperature is 20-45 DEG C, and collecting filtrate; and step 5, carrying out corrosion prevention and drying on the filtrate to obtain the chickpea sprout peptide. The chickpea sprout peptide has the effects of resisting obesity, resisting oxidation and adjusting immunity, in addition, the preparation method can achieve efficient and high-quality preparation of the chickpea sprout peptide, the chickpea sprout peptide is accurately screened, and the ineffective experiment cost is reduced.

The invention relates to a medicinal and edible fermentation broth for promoting blood circulation and improving cold in four limbs and a preparation method thereof, the preparation method comprises the following steps: step 1, pulping: moistening a medicinal and edible composition, adding water, and pulping to obtain medicinal and edible slurry; wherein the medicinal and edible composition comprises Chinese dates, rhizoma polygonati, Chinese wolfberry fruits, codonopsis pilosula and longan; step 2, enzymolysis and sterilization: performing enzymolysis on the medicinal and edible slurry, and sterilizing to obtain a fermentation substrate; step 3, fermentation and solid-liquid separation: inoculating composite probiotics into the fermentation substrate for fermentation culture, and after the culture is finished, carrying out solid-liquid separation to obtain medicinal and edible fermentation liquor; wherein the compound probiotics are prepared from lactobacillus plantarum LP45 and lactobacillus rhamnosus LR519. The problem of cold hands and feet can be safely and effectively solved.

The invention belongs to the field of biological material extraction, and particularly relates to a method for extracting collagen peptide from marine organisms, which comprises the following steps: S1, crushing marine organism raw materials, soaking the crushed marine organism raw materials in a citric acid buffer solution containing alginate oligosaccharide for swelling treatment, and then performing high-voltage pulse electric field treatment to obtain pretreated collagen slurry; and S2, carrying out first-stage enzymolysis treatment on the pretreated collagen slurry through alkaline protease, and then carrying out second-stage enzymolysis treatment through collagenase and flavourzyme. S3, performing separation treatment through a first ultrafiltration membrane to obtain first filtrate, performing deodorization treatment on the first filtrate through a first nanofiltration membrane of which the surface is coated with a beta-cyclodextrin adsorbent to obtain second filtrate, and performing separation treatment on the second filtrate through a second nanofiltration membrane to obtain third filtrate. And S4, concentrating, desalting and drying the third filtrate to obtain collagen peptide powder. Through the synergistic effect of multiple steps, the concentration of the molecular weight of the collagen peptide and the efficient deodorization are realized on the basis of retaining the active structure of the collagen.

The invention belongs to the field of traditional Chinese medicine preparations, and particularly relates to an easy-to-absorb oral liquid capable of promoting qi-blood tonifying and a preparation process thereof. Comprising the following steps: preparing an absorption promoter; preparing a stable compatilizer; steaming and extracting the traditional Chinese medicinal materials; and preparing the oral liquid. The method comprises the following steps: carrying out enzymolysis on mixed slurry prepared from asparagus flowers and resina liquidamberis by utilizing cellulose-pectin compound enzyme, freeze-drying the prepared resina liquidamberis-asparagus flower enzymatic hydrolysate to obtain an absorption enhancer, and adding the absorption enhancer in the process of preparing the oral liquid, the absorption rate of intestinal tracts to active ingredients of Chinese wolfberry fruits, poria cocos, Chinese dates and longan aril can be increased, and the effect of promoting qi and blood tonifying and easy absorption of the oral liquid on blood and qi tonifying of human bodies is improved.

The invention discloses a collagen injection for tendon repair as well as a preparation method and application thereof, and belongs to the technical field of collagen preparation. The preparation method disclosed by the invention comprises the following steps: by taking a collagen dry product as a raw material, sequentially dissolving, carrying out secondary filtration, separating, preparing fibrotic collagen slurry A, preparing gelatinized collagen slurry B, preparing the solution, homogenizing and filling to prepare the collagen injection capable of being used for tendon repair. The collagen injection provided by the invention shows multiphase transformation during refrigeration and contact with body fluid with body temperature, and does not have the phenomenon of fiber layering in the long-term storage process. The collagen disclosed by the invention is an injection type gel preparation, can be flexibly injected and applied according to the depth and the area of a wound, is beneficial to promoting tissue regeneration and functional repair, and is particularly suitable for tendon injury and other scenes needing collagen to guide cell repair.

The invention provides a pressure sore staging judgment method and device based on image recognition, and the method comprises the steps: segmenting a pressure sore image from a collected image of an affected part; identifying pressure sore features from the pressure sore image; wherein the pressure sore characteristics comprise pressure sore color, tissue type and area and skin integrity; the tissue types at least comprise spots, serous vesicles, granulation tissues, fat, slough, eschar, fascia muscles, tendons, ligaments, cartilages and bones; and determining stages of the pressure sores according to the identified features of the pressure sores. According to the invention, the pressure sore staging can be automatically identified based on the pressure sore features in the pressure sore image, and the accuracy and efficiency of pressure sore staging identification are improved.

The present invention discloses an advanced small conduit pre-reinforcement system, including an advanced small conduit, wherein the advanced small conduit is provided with a double layer along the entire circumference of the tunnel, wherein the double layer includes an inner layer and a middle layer, wherein the inner layer and the middle layer of the advanced small conduits are uniformly inserted in the water-rich soft rock along the circumference of the tunnel, wherein the inner layer of the advanced small conduits and the middle layer of the advanced small conduits are staggered, wherein an outer layer of the advanced small conduits is added within an angle range of 110°-130° of the top pressure arch of the tunnel, wherein the outer layer of the advanced small conduits are uniformly inserted in the water-rich soft rock along the circumference of the tunnel, wherein the outer layer of the advanced small conduits and the middle layer of the advanced small conduits are staggered, wherein grouting holes are uniformly provided on the advanced small conduits, wherein the advanced small conduits are used for injecting grouting liquid, and wherein the ends of the advanced small conduits are fixed on the arch frame of the tunnel. The present invention enables a large grouting reinforcement area, a good reinforcement effect, effectively reinforces loose rock mass, prevents tunnel collapse, and thus improves surrounding rock grade and construction safety.

The invention relates to the technical field of modernization of traditional Chinese medicines, in particular to a nano-vesicle, a preparation method and application of the nano-vesicle in preparation of an endometrial injury repairing medicine. The preparation method comprises the following steps: taking a periplaneta americana ethanol extracting solution, and adding a buffer solution to prepare diluted homogenate; performing differential centrifugal treatment on the homogenate; filtering the supernatant obtained by the differential centrifugation treatment, and then centrifuging for 60-120 minutes by 100000g of the supernatant to obtain a precipitate; and re-suspending the precipitate to obtain the periplaneta americana ethanol extract source nano-vesicles. The periplaneta americana ethanol extract is used as a starting material to enrich the nano-vesicles for the first time, the combined process of differential centrifugation, membrane filtration and ultracentrifugation is adopted, the obtained nano-vesicles can be applied to the field of endometrial injury repair, especially treatment of gynecological diseases such as intrauterine adhesion and infertility with thin intima, and the blank in the technical field is filled.

Provided in the present disclosure are materials, devices, systems, and methods for performing cryo-surgery in a mammalian subject. More particularly, presented are a slurry, devices, systems, and methods for subcutaneously injecting the slurry into a patient in need of bodyfat reduction intervention. The slurry, devices, systems, and methods permit highly efficacious subcutaneous ice phase-change lipolysis while not requiring that the patient be placed under general anesthesia, nor requiring significant new surgical training among practitioners.

Provided is a technology for improving the efficiency of separating a complex of a protein to be purified and a carrier in a hydrocyclone of a device including an in-line mixer and the hydrocyclone. A protein purification device disclosed herein comprises a first supply unit, a second supply unit, an in-line mixer, a hydrocyclone, and a dilution line. The first supply unit supplies a first slurry containing a carrier including a ligand that binds to a protein. The second supply unit supplies a second slurry which is a cell culture solution containing a protein. The in-line mixer mixes the first slurry and the second slurry. The hydrocyclone separates, downstream of the in-line mixer, a complex of the protein and the carrier from a mixed slurry of the first slurry and the second slurry. The dilution line is provided between the in-line mixer and the hydrocyclone and introduces a diluted liquid into the mixed slurry.

The invention relates to the field of agricultural production, in particular to a plant nutrition preparation and a preparation method thereof. The preparation method of the plant nutrition preparation comprises the following steps: (a) pulping a mixed solution containing sea cucumber visceral tissues and a thiol compound to obtain slurry; (b) adding protease into the slurry, performing enzymolysis, filtering and diluting to obtain a micromolecular collagen peptide solution; and (c) adding nutrient elements into the heated micromolecular collagen peptide solution, and then carrying out chelation reaction. The preparation method of the plant nutrition preparation has the advantages of being environmentally friendly, easy to operate, low in cost and the like, the obtained plant nutrition preparation can provide various nutrient substances for plant growth and promote plant growth, and reasonable utilization of waste sea cucumber resources is achieved.

Disclosed is device comprising first conduit (301) for supplying aqueous alginate-containing slurry; first pressurizing means (302) configured for sustaining flow of aqueous alginate-containing slurry through an outlet (304) of first conduit, wherein outlet comprises a flow distributor having at least one aperture (305) through which flow of the aqueous alginate-containing slurry exits; supply (306) for aqueous calcium-containing solution connected to outlet of first conduit to, in operation, contact aqueous alginate-containing slurry exiting the at least one aperture and cure at least a skin part of alginate-containing slurry to obtain a skin-cured shape; dividing element (307) having at least one dividing blade (308) configured to pass over the at least one aperture of the flow distributor at defined distance and divide skin-cured shape exiting the at least one aperture into number of skin-cured pieces thereby forming non-animal- based fibrous product; and receptacle (309) for receiving non-animal-based fibrous product. Method is also disclosed.

The invention discloses a rapid drug sensitivity detection method based on fish tissue homogenate, which comprises the following steps: taking fish tissues for homogenate, coating and inoculating the obtained homogenate on a flat plate, placing a drug sensitivity paper sheet, culturing for 16-24 hours at 30 DEG C, then measuring the diameter of an inhibition zone on the flat plate, and judging a drug sensitivity detection result. According to the method, the sample pretreatment and detection process is simplified, diseased fish tissue homogenate is directly used, the detection period is remarkably shortened to be within 1 d, and meanwhile the result accuracy is ensured. According to the technical scheme, the method comprises the steps of sample collection under the sterile condition, tissue homogenate preparation, homogenate coating inoculation, drug sensitive paper sheet placement and culture result interpretation. The method has the advantages of high efficiency, rapidness, simplicity and convenience in operation, accurate result and wide application range, is suitable for drug sensitivity detection of various fish pathogenic bacteria, and has important practical application value.

The invention relates to the technical field of geological reinforcement, and discloses a grout stopping method for stratum pre-grouting reinforcement, which adopts an A and B double-component grout system: the B component contains thermal stability urease inhibited by a reversible inhibitor; the component A contains a corresponding inhibitor and a corresponding scavenger, and the two components are mixed to release the reaction through inhibition, so that the controllable prolonging of the slurry gelation time is realized, and the blockage of a pipeline is avoided. A self-indication and slow-release carrier loaded with copper ions is arranged in the component A, after the slurry reaction is started, ammonium ions of a reaction product replace the copper ions, the blue change of the slurry is caused, and a visual basis is provided for judging the injection stopping opportunity. The component A further comprises an energy field response medium and reserve urea packaged in the carrier, and the energy field response medium and the reserve urea enable the consolidation body to have the later non-contact remote control enhancement capacity and the micro-crack passive self-repairing capacity respectively. The grouting process is safe and controllable, the reaction state is visible in real time, and the performance of the consolidated body is regulated and controlled in the later period.

The present disclosure provides novel compounds, compositions, and methods for modulating one or more septin proteins, e.g., septin-2. Such compounds and compositions are useful for the treatment of a cancer, e.g., endometrial, pancreatic, lung, breast or ovarian cancer, such as serous and ovarian clear cell carcinoma.

A guided mode resonance (GMR) sensor that can be used to simultaneously detect an array of analytes. It provides a diagnostic system that can rapidly detect an array of biomarker proteins in patient samples (such as blood, serum or plasma for example) which can be used as an accurate means to conduct a differential analysis of proteins that allows the discrimination of early and late stages of disease, such as metastatic versus primary ovarian serous carcinomas. The GMR sensor can be provided in a compact size such that it can be portable.

Cryogranulation systems with improved dispenser assemblies are provided for use in manufacturing frozen pellets of pharmaceutical substances in a fluid medium. Methods of cryogranulating the pharmaceutical substance in the fluid medium are also provided. In particular embodiments, the dispenser assembly is used with suspensions or slurries of pharmaceutical compositions including biodegradable substances, such as proteins, peptides, and nucleic acids. In certain embodiments, the pharmaceutical substance can be adsorbed to any pharmaceutically acceptable carrier particles suitable for making pharmaceutical powders. In one embodiment, the pharmaceutical carrier can be, for example, diketopiperazine-based microparticles. The dispenser assembly improves the physical characteristics of the cryopellets formed and minimizes product loss during processing.

The invention provides a subretinal effusion region segmentation and volume calculation method and system, a product and a terminal, and the method comprises the steps: automatically segmenting a fundus prediction lesion region image based on a pre-constructed fundus lesion region prediction model, and carrying out the summation of pixel points, belonging to a subretinal effusion part, in the data of each fundus prediction lesion region image. According to the method, the volume data of the subretinal fluid is obtained, the film reading time of a fundus disease doctor is greatly shortened, the working efficiency is improved, in addition, the film reading accuracy, stability and homogeneity can be remarkably improved, and clinical doctors can be helped to accurately and objectively evaluate the central serous chorioretinopathy.

The application discloses a bird's nest compound product with the effect of improving female body health and a preparation method and application thereof. The preparation method comprises the following steps: mixing red ginseng, Gastrodia elata, ophiopogon, donkey-hide gelatin, eucommia and ganoderma lucidum with a solvent to form a mixed slurry; treating and extracting the mixed slurry by applying an electric field to prepare a compound liquid; and mixing the compound liquid with bird's nest to prepare the bird's nest compound product with the effect of improving female body health. The bird's nest is matched with the medicine-food homologous material, and on the basis of the theory of "suo zhuo jun chen", the bird's nest compound product capable of obviously improving menopausal syndrome is prepared, the hormone level is effectively adjusted, symptoms such as hot flashes, night sweats, sleep quality improvement, ovary and uterus repair promotion and the like are relieved, and the product is mild in action, high in safety, good in flavor and color.

The invention provides a dual-protein preparation and application thereof in hair care products. A preparation method of the dual-protein preparation comprises the following steps: A, extracting collagen; b, extracting keratin; and C, mixing the collagen and the keratin to prepare the dual-protein preparation. A collagen extraction process in the step A comprises the following steps: a, firstly, cleaning and airing cod skin; b, the treated cod skin is added into a shearing machine to be sheared into fragments, the fragments are put into a stainless steel barrel, enzymatic hydrolysate is added for enzymolysis, and enzymolysis mixed slurry is obtained after uniform stirring; c, adding the enzymolysis mixed slurry into an ultrasonic machine, and carrying out pneumatic ultrasonic extraction to release water-based protein so as to form cod skin active mixed liquid; according to the preparation method, a protein preparation can be obtained through rapid and accurate extraction, the performance of hair care products can be effectively improved, and the content of disulfide bonds in hair is increased.

The present application relates to the field of traditional Chinese medicine, in particular to a kind of traditional Chinese medicine composition for treating central serous chorioretinopathy and application thereof.The present application provides a kind of traditional Chinese medicine composition for treating central serous chorioretinopathy, compared with the prescription of Chunzai Decoction, the traditional Chinese medicine composition of the present application increases the efficacy of promoting blood circulation and removing blood stasis, soothing the liver and relieving depression, clearing the liver and improving eyesight, clearing heat and reducing phlegm on the basis of Chunzai Decoction warming yang, transforming qi, reducing water and removing dampness;Compared with using Chunzai Decoction alone, the curative effect of patients using the traditional Chinese medicine composition is more direct, from 2-3 weeks to 1 week, and the curative effect is accelerated.And the traditional Chinese medicine composition of the present application has been used for about 2 years, 2 / 3 of the 112 patients can see effect in 1 week, and no recurrence is found within 3 months.

The present invention provides a method of treating high-grade serous cancer in a patient in need thereof, comprising administering to the patient in need thereof an effective amount of an SIK2 / 3 inhibitor or a pharmaceutically acceptable salt thereof, where the effective amount is lower than the amount required to directly kill tumor cells. The method of treating high-grade serous cancer in immune-functioning patients in need thereof may also include administering an immune checkpoint inhibitor.

The application discloses a kind of livestock product drug residue detection methods based on near infrared spectroscopy technology, it is related to trace detection field, the detection method includes the following steps: S1, livestock product sample is mixed with buffer solution, and obtain homogenate liquid;S2, hydroxylamine hydrochloride solution is added to homogenate liquid, and oscillation reaction is obtained under mild conditions, and reaction liquid is obtained;S3, nanometer gold stick dispersion liquid is added to reaction liquid, and mixed liquid is obtained;S4, near infrared spectrum of mixed liquid is collected;S5, pretreatment is carried out, and local surface plasmon resonance peak position is extracted, and peak position offset is calculated;S6, peak position offset is substituted into quantitative model, and drug residue concentration is calculated.The application realizes the in-situ release of total residue by selective rupture of the covalent bond between drug and protein, and realizes the high-sensitivity detection of sulfonamides, tetracyclines and fluoroquinolones under the condition of extraction-free by combining the signal amplification effect of nanometer gold stick.

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus gasseri KB24 and application thereof in preparation of cattle spleen peptide for improving spleen deficiency. The preparation method comprises the following steps: (1) pretreatment: removing envelopes of the cattle spleen, crushing, and homogenizing to prepare cattle spleen slurry; (2) enzymolysis treatment: adding a compound enzyme into the cattle spleen slurry for enzymolysis, and sterilizing after enzymolysis to obtain an enzymatic hydrolysate; (3) performing fermentation treatment: inoculating lactobacillus gasseri KB24 into the enzymatic hydrolysate, and performing fermentation to obtain fermentation liquor; and (4) carrying out solid-liquid separation, filtration, ultrafiltration membrane separation and freeze drying on the fermentation liquor to obtain the cattle spleen peptide. The cattle spleen peptide prepared by the method can improve the spleen deficiency symptom from the aspects of weight, holding power, exhaustive swimming time, gastrointestinal digestive function, energy substance metabolism and the like, and can be applied to products for improving spleen deficiency.