113 results about "Intrauterine adhesion" patented technology

An apparatus and method of use or treatment are disclosed for creating intrauterine adhesions resulting in amenorrhea. In particular, the apparatus relates to an easily deployed intrauterine implant that readily and consistently reduces or eliminates abnormal intrauterine bleeding. In addition, the apparatus is also used as a uterine marker device for visualizing endometrial tissue thickness and potential changes. The method of the present invention serves as a supplement to or a replacement for conventional hysterectomy or ablation/resection procedures used to treat menorrhagia.

The invention relates to a preparation method and application of collagen scaffold composite bone marrow-derived mesenchymal stem cells (BMSCs). The preparation method comprises the steps of preparing single cell suspension after trypsinizing the BMSCs, uniformly dropwise adding the single cell suspension to a collagen scaffold, putting the collagen scaffold into an incubator to be cultured, and adding an L-DMEM complete medium to continue culture, thus obtaining the collagen scaffold composite BMSCs. The preparation method has the advantages that the following defects are overcome: endometria are seriously injured as intrauterine adhesion is mechanically separated by adopting hysteroscopic surgery, intrauterine devices or anti-adhesion materials are put after the surgery and estrogens are given after the surgery to promote intima growth; the problem of intima scars can not be solved; functional intima repair can not be achieved; adhesion is very easy to happen again. As active ingredients for treating serious endometrium injury, the BMSCs are convenient to obtain, secrete growth factors to improve the local microenvironment and immunoregulation, have good biocompatibility, degradability and safety, promote scarred endometrium repair and increase the intima thickness and local blood vessel density.

The invention discloses a medical anti-adhesion membrane material with controllable degradation and a preparation method thereof, belonging to the field of biomedical materials. According to the invention, the membrane material is prepared by blending sodium hyaluronate and konjac klucomannan, using lactic acid as a property-modifying additive and using a mixed solution of ammonia water and anhydrous ethanol as a cross-linking agent; sodium hyaluronate is a good anti-adhesion material, but individually used sodium hyaluronate has a fast degradation speed and can not fulfill a good anti-adhesion effect; konjac klucomannan can control the degree of cross-linking, thereby realizing the goal of controllable degradation, and blending of sodium hyaluronate and konjac klucomannan enables sodium hyaluronate to be released slowly, thereby allowing the membrane material to meet demands for anti-adhesion action at different parts. The membrane material has the advantages of nontoxic degradation products, good biocompatibility, hygroscopicity and tensile properties, and the like, and can be used for intervertebral adhesion, intestinal adhesion, skin adhesion, intrauterine adhesion, etc.

The invention discloses a preparation method of modified silicone rubber. The method comprises the following steps: by using silicone rubber as a raw material, adding 0.1-20wt% of estrogen, stirring for 5-30 minutes at room temperature, and uniformly mixing; then, adding a mixed vulcanizing agent to the mixed system, continuously stirring for 5-20 minutes and then paving a film under panel glass and drying; finally, vulcanizing and furnace-cooling at 100-300 DEG C. The silicone rubber prepared by the method has the function of preventing intrauterine adhesion and accelerating the recovery of endometrium.

The invention relates to an intrauterine postoperative anti-adhesion instrument capable of degrading completely. The intrauterine postoperative anti-adhesion instrument capable of degrading completelycomprises a stent and a drug coating, wherein the stent is formed by a completely degradable polymer, the drug coating is loaded to the outer surface and/or the inner surface of the stent for promoting the repair of the endometrium and preventing adhesion, and the stent has a non-binding state before implantation, a compression state during implantation and an unfolded state after implantation; the stent is of a tubular structure which is unfolded around a central longitudinal axis in a non-binding state before implantation, and the first diameter of the top of the cylindrical structure is larger than the second diameter of the bottom; in a compressed state during implantation, the stent is of a tubular structure which is compressed around the central longitudinal axis, and the diameter of the tubular structure is smaller than that of the second diameter; the stent is a hollow flat sheet in an unfolded state after implantation. According to the intrauterine postoperative anti-adhesioninstrument capable of degrading completely, the intrauterine adhesion caused by wounds after various intrauterine operations can be treated, and the intrauterine postoperative anti-adhesion instrument is particularly suitable for secondary adhesion after the intrauterine adhesion separation operation.

The invention provides an intrauterine adhesion prevention and treatment device. The intrauterine adhesion prevention and treatment device comprises a triangular balloon, a balloon catheter, a catheter base, a balloon support and medicine sustained-release capsules, wherein the balloon catheter is connected with the first vertex of the balloon and communicated with the interior of the balloon, the catheter base is connected with the end, away from the balloon, of the balloon catheter, the medicine sustained-release capsules are used for storing medicine and releasing the medicine gradually in the vivo environment, the two ends of the balloon support are connected with the second vertex and the third vertex of the balloon respectively, a position point of the balloon support is located at the first vertex of the balloon, and the medicine sustained-release capsules are arranged at the two ends of the balloon support. By means of the intrauterine adhesion prevention and treatment device, intrauterine adhesion can be effectively prevented in a short term or a long term, a second operation for replacing an intrauterine device is not needed any more, and the problem that restoration of the endometrium of a patient in whom estrogenic hormones and progestational hormones are contraindicated is slow because the patient can not take the hormones orally is also solved.

The invention discloses an intrauterine adhesion prevention and cure device, which comprises an intrauterine-shaped ring and a film, wherein the intrauterine-shaped ring has elasticity; the shape and the size of the intrauterine-shaped ring are adaptive to the periphery of the inner wall of a uterus; the film is connected to the left inner side and the right inner side of the intrauterine-shaped ring; and the middle of the film is provided with an opening from the bottom to the lower section of the intrauterine-shaped ring. According to the intrauterine adhesion prevention and cure device, the intrauterine is prevented from adhering again, liquid convection in the intrauterine can be increased, and infection caused by unsmooth drainage is avoided. In addition, the intrauterine adhesion prevention and cure device also has the beneficial effects on saving raw materials, lowering manufacture cost and saving treatment cost for patients.

The invention discloses a collagen dermis material for promoting endometrium repairing and a preparation method of the collagen dermis material. For the collagen dermis material for promoting endometrium repairing and the preparation method of the collagen dermis material, the preparation method comprises the following step: soaking collagen in a polypeptide solution, thus obtaining the collagen material, wherein the polypeptide comprises a collagen binding domain, an MSCs aggregation promoting domain, and connecting peptide positioned between the collagen binding domain and the MSCs aggregation promoting domain. The experiment proves that the collagen membrane material prepared by adopting the method provided by the invention has good biocompatibility, good degradability, low immunogenicity and the like, and can effectively promote the aggregation and differentiation of human mesenchymal stem cells (hMSCs), the introduced polypeptide modification can increase the binding, aggregation and differentiation of MSCs, and thus an effective barrier function is achieved for intrauterine adhesion.

The invention provides a degradable gasket with anti-bacterial anti-adhesion performance. The degradable gasket is prepared via weaving or knitting of a plurality of bundles of magnesium-based metal wire, or via punching of magnesium-based metal sheets. The magnesium-based metal wire can be made of pure magnesium or a magnesium-based alloy of different elements based on disease characteristics and treatment requirements. The degradable gasket possesses excellent biocompatibility; in vivo degradation and absorption can be realized; degradation speed can be controlled; the degradable gasket is capable of preventing postoperative adhesion and infection, and promoting wound healing, can be used for preventing epidural adhesion after orthopedic surgery laminectomy, adhesion after tendon rupture repairing operation, adhesion after knee surgery, dura adhesion after transcranial surgery, eye socket soft tissue adhesion, intestinal adhesion after abdominal surgery, and intrauterine adhesion, is capable of reducing infection risk of the above surgeries, and possesses significant clinical application value.

The invention discloses an application of hsa_circ_0003764 as a marker to preparation of a diagnosis preparation for intrauterine adhesion. The expression of hsa_circ_0003764 in endometrium cicatricial tissue of patients suffering from intrauterine adhesion is notably increased, and the hsa_circ_0003764 can resist digestion of ribonucleic acid (RNA) excision enzymes R ( RNase R), and more stable than liner RNA. In vitro cell experiment proves that the hsa_circ_0003764 can cause endometrial stromal apoptosis and epithelial fibrosis. si-hsa_circ_0003764 has the effect of resisting epithelial fibrosis. Therefore, the hsa_circ_0003764 can be used as the marker for diagnosis of the endometrial stromal so as to prepare a diagnosis reagent for diseases caused by uterus fibrosis. The invention relates to an application of the reagent for detecting the hsa_circ_0003764 to preparation of a diagnosis reagent of the intrauterine adhesion.

The invention provides an intrauterine compression negative pressure hemostatic inflation balloon. The inflation balloon includes a drainage assembly and a hemostatic assembly; the drainage assembly includes a first balloon, drainage blocks, a first drainage catheter, a second drainage catheter, a drainage bag, a third drainage catheter, an air inlet tube, a filtering screen, a first valve, a first one-way valve, a connector, a second one-way valve, an airbag and a connecting tube; the hemostatic assembly includes a second balloon, a liquid injecting tube, a shunting tube, a liquid dischargingtube, a second valve and a third one-way valve; the drainage blocks are installed inside the first balloon; the tops of the drainage blocks are provided with drainage holes, and the side surfaces ofthe drainage blocks are provided with through holes; the first drainage catheter communicates with one sides of the drainage blocks; one end of the connecting tube communicates with one side of the first drainage catheter, and the other end of the connecting tube communicates with one end of the second drainage catheter; and the other end of the second drainage catheter communicates with the top of the drainage bag. Through the arrangement of the drainage assembly and the hemostatic assembly, the inflation balloon can have the effects of stopping bleeding and preventing intrauterine adhesion.

The invention belongs to the field of biological medicine, and particularly relates to application of menstrual blood stem cells in preparation of drugs for treating intrauterine adhesion. A preparation method of the menstrual blood stem cells comprises the following steps: diluting an extracted menstrual blood sample by using PBS, slowly adding to the upper layer of a nuclear red blood cells membrane, centrifuging and separating a mononuclear cell layer, removing supernate, resuspending a complete culture solution for cell precipitation, inoculating in a cell culture bottle, and culturing in a culture box with 5% of CO2 at the temperature of 37 DEG C; when cells grow all over to 80-90%, carrying out digestion passage with 0.25% of trypsin, and after cytoplasm retracts and gaps among cells are increased, adding the complete culture solution to terminate digestion; repeatedly blowing and beating a bottle wall to form cell suspension; sucking the cell suspension into a centrifuge tube, centrifuging and removing supernate; adjusting cell concentration by the complete culture solution for cell precipitation, and inoculating in a culture bottle; and culturing in a culture box with 5% of CO2 at the temperature of 37 DEG C, wherein culture passage numbers are 2-3. The cells are high in survival rate, stable in character, easy to obtain and easy to amplify, and has remarkable treatment effect on intrauterine adhesion.

The invention discloses applicationof let-7c/let-7d in intrauterine adhesion and/or thin endometriumdiagnosis and treatment. It is discovered that for an intrauterine adhesion patient, let-7c/let-7d expression in the endometrium is remarkably reduced. In-vitro experiments show that after the let-7c/let-7d is reduced, fibroses endometrial epithelium cells are increased. In-vivoanimal experiments show that by supplementing the let-7c/let-7d, the endometriumfibrosis degree of an endometriumfibrosis mouse is remarkably reduced. Therefore, the let-7c/let-7d possiblyplay an importanteffect in the aspects of intrauterine adhesion and/or thin endometriumdiagnosis and treatment, and can be used for preparing medicine for treating intrauterine adhesion and/or thin endometriumdiagnosis and treatmentor preparing medicine for treating other diseases related to uterusfibrosis.

The invention relates to a uterine cavity implant as well as a preparation method and application thereof. The uterine cavity implant is characterized by having a bagged structure. The uterine cavity implant comprises a substrate material of animal small intestine submucosa subjected to decellularization treatment. An implantable medical device for preventing and treating intrauterine adhesion comprises a biological tissue substrate material. The present decellularization technology for a biological repairing material is improved; compared with the present product, the biological tissue substrate material provided by the invention has the advantages of lower DNA residual quantity, lower immunogenicity, higher anti-infection capacity and higher repairing capacity; and the uterine cavity implant is beneficial to the recovering of tissues, such as, uterine cavity base layer, submucosa and mucous layer. Besides, the invention prepares the biological repairing material into the uterine cavity built-in structure which is used for isolating wound surfaces, repairing uterine cavity tissues and reducing scar forming, so that the intrauterine adhesion problem is solved, and the accordingly generated sterility problem also can be solved.

The invention discloses a preparation method of a cellular composite endometrial repair gel. The method comprises the following steps of 1. extracting and culturing endometrial stem cells to obtain endometrial stem cells; 2, extracting and culturing mesenchymal stem cells to obtain the mesenchymal stem cells; 3, preparing temperature-sensitive chitosan hydrogel; and 4, preparing the cellular composite endometrial repair gel. The invention also discloses application of the cellular composite endometrial repair gel in treating intrauterine adhesion. The invention has the following advantages that 1, autologous stem cell transplantation basically eliminates the risk of rejection; 2, the autologous endometrial stem cells and the mesenchymal stem cells are subjected to composite transplantation, so that regeneration of endometrium, microvessels and mesenchyme is accelerated, and the endometrium repair time is obviously shortened; and 3, the temperature-sensitive gel can be formed after local injection, which can only only provide a three-dimensional retention space for the stem cells, but also prevent the adhesion of regenerated mucous membranes.

An intrauterine adhesion blocker comprises a V-shaped framework which is corresponding to a uterine cavity form and a base rod; the V-shaped framework comprises a first framework arm and a second framework arm; a first end of the first framework arm and a first end of the second framework arm are connected at the lower end of the V-shaped framework; the first framework arm and the second framework arm can mutually get close in an elastic mode to close the V-shaped framework into a slender shape; a degradable mesh is formed between the first framework arm and the second framework arm; the base rod is formed at the lower end of the V-shaped framework and extends downward from a connecting joint point of the first end of the first framework arm and the first end of the second framework arm.

The invention discloses a preparation method of 3D printing hydrogel for treating or preventing intrauterine adhesion. The method comprises the following steps: adding methacrylic acid gelatin, methacrylic acid collagen and a photoinitiator into deionized water, and uniformly mixing to obtain printing ink; designing a model by using 3DS Max software, and importing the model into 3D printer control software; transferring printing ink and human amniotic mesenchymal stem cells into an extrusion cylinder of a 3D printer, printing to obtain a primary hydrogel product, and irradiating and curing the primary hydrogel product with blue light to obtain the 3D printing hydrogel for treating or preventing intrauterine adhesion. The 3D printing hydrogel disclosed by the invention has excellent biocompatibility and in-situ crosslinking capability, controllable mechanical property, swelling property and degradability and high cell encapsulation efficiency, hAMSC can be loaded inside the hydrogel, controllable release of hAMSC can be realized, hAMSC can be continuously released in vitro for more than 7 days, and intrauterine adhesion can be effectively treated or prevented by utilizing hAMSC.

The invention belongs to the field of medical apparatus and instruments and particularly relates to bendable hoe-blade-shaped surgical instrument specially used for removing an intrauterine adhesion scar under a minimally invasive surgery hysteroscope. The bendable hoe-blade-shaped surgical instrument comprises a steel tube with the outer diameter being 3mm and is characterized in that a plane where the included angle of a blade handle and a blade coincides with a plane where a bent bendable joint tube is located, and the angle sum of the bending angle of the bendable joint tube and the included angle of the blade handle and the blade is 180 degrees. The bendable hoe-blade-shaped surgical instrument has the advantages that the intrauterine adhesion scar can be thoroughly removed under theminimally invasive surgery hysteroscope, and single-hand operation and multi-angle operation by operation staff are facilitated by the coordination of an operation handle and an auxiliary handle and the lock catch of the auxiliary handle; the defects that the removing of the intrauterine adhesion scar in intrauterine adhesion surgery is troublesome and not thorough and the damages the endometriumare overcome, the scar can be removed conveniently, surgery time can be shortened, and the endometrium can be protected; the bendable hoe-blade-shaped surgical instrument is simple in overall structure, convenient to use and maintain and low in manufacturing cost.

The invention discloses an intrauterine injection traditional Chinese medicine preparation capable of preventing intrauterine adhesion and a preparing method of the intrauterine injection traditional Chinese medicine preparation. The intrauterine injection traditional Chinese medicine preparation capable of preventing intrauterine adhesion is composed of, by weight, 10 to 15 parts of salvia miltiorrhiza, 10 to 15 parts of angelica sinensis, 10 to 15 parts of ligusticum wallichii, 8 to 10 parts of sophora flavescens, 8 to 10 parts of scutellaria baicalensis, 8 to 10 parts of coptis chinensis, 8 to 10 parts of golden cypress, 8 to 10 parts of atractylodes, 8 to 10 parts of dandelions, 8 to 10 parts of honeysuckle, 8 to 10 parts of forsythia, 8 to 10 parts of red paeony roots, 8 to 10 parts ofraspberries, 8 to 10 parts of sargentodoxa cuneata, 8 to 10 parts of patrinia scaniosaefolia, 8 to 10 parts of houttuynia cordata, 3 to 5 parts of trigone, 3 to 5 parts of curcuma zedoary and 2 to 3 parts of honey-fried licorice roots. The intrauterine injection traditional Chinese medicine preparation capable of preventing intrauterine adhesion is prepared through various types of traditional Chinese medicine in a blended mode and has the advantages of being simple in operation, low in recurrence rate and free of toxic and side effects.