88 results about "Intrauterine adhesion" patented technology

The invention belongs to the field of biological pharmacy, and relates to a temperature-sensitive hydrogel, in particular to a temperature-sensitive hydrogel SV (at) HP based on hyaluronic acid as well as a preparation method and application of the temperature-sensitive hydrogel SV (at) HP. The temperature-sensitive hydrogel SV (at) HP entraps CS-siRNA nanoparticles and VEGF (at) PLGA microspheres, and is used for realizing microenvironment responsive drug delivery to treat intrauterine adhesion. Chlorogenic acid and TGF beta1 siRNA are self-assembled through disulfide bonds to form CS-siRNA nano-particles, the CS-siRNA nano-particles are used for treating intrauterine adhesion through gene and chemical therapy, and cell and animal experiments prove that the CS-siRNA nano-particles have a good effect of improving intrauterine adhesion. Through evaluation of a real-time fluorescence quantification method, a western blot method, a cell scratch experiment, laser confocal microscope observation, inverted fluorescence microscope observation, an in-vivo experiment and the like, the SV-coated HP gel has the effects of resisting inflammation, improving endometrial fibrosis, promoting IUA rat endometrial angiogenesis and repair, inhibiting intrauterine adhesion and improving the intrauterine environment.

The intrauterine adhesion therapeutic apparatus comprises two skeleton layers, the two skeleton layers are symmetrically arranged to form a uterus-shaped ring, and the uterus-shaped ring is of a bionic uterus structure; each skeleton layer comprises a first supporting piece and a second supporting piece, the first supporting piece and the second supporting piece are arranged in a hinged mode, a longitudinal rod is hinged to the top ends of the two skeleton layers, the intrauterine adhesion therapeutic apparatus further comprises an outer sleeve, a connecting pipe is fixedly arranged at the bottom of the outer sleeve, and the bottom ends of the two skeleton layers are hinged to the connecting pipe. The outer sleeve sleeves the outer part of the longitudinal rod in a sliding manner; an opening is formed in the bottom of the longitudinal rod, internal threads are arranged at the bottom of the longitudinal rod, the longitudinal rod is connected with a push assisting rod through the internal threads, nerves of the cervix uteri and the private part can be continuously anesthetized through medicine, the postoperative analgesia effect is achieved, and meanwhile needed medicine can be injected through the device for further treatment.

The invention belongs to the technical field of biological medicines, and particularly discloses an insulin-like growth factor-1-linker polypeptide-collagen, and the linker polypeptide has an amino acid sequence as shown in SEQ ID No.1. The N end of the designed and synthesized linker polypeptide simulates an IGF-1 receptor structure and can be combined with IGF-1, the C end of the linker polypeptide is rich in lysine residues and can be covalently cross-linked to collagen through glutamine transaminase, the attachment time of the IGF-1 in endometrium is prolonged, and endometrium thickening and endometrium injury repair are effectively promoted. The protein solution for promoting endometrial injury repair provided by the invention can form a stable repair microenvironment on the surface of the endometrial, promote the number recovery of endometrial glands with intrauterine adhesion, reduce the fibrosis ratio and promote the thickening of thin endometrial, has the advantages of high histocompatibility, simplicity in preparation, obvious improvement effect and the like, and can be used for preparing a medicine for treating endometrial injury. The method has no potential toxic influence on cells and has a good clinical application prospect.

The invention discloses an effectiveness analysis method of a drug entrapment material for treating intrauterine adhesion. The effectiveness analysis method comprises the following steps: performing multi-aspect research on the drug entrapment material and generating an effectiveness analysis result. In performance characterization, the morphology and pores of the material are analyzed by using microscopy, specific surface area and other technologies, the compatibility of the drug and the carrier is verified, and the physical state of the drug is detected; in-vitro analysis covers drug release kinetics, the release amount is measured in a simulated environment, and a curve is fitted; the biocompatibility evaluation refers to co-culture of a material leaching solution and cells, detection of cell activity and the like; implanting a medicine carrying material into the animal model in vivo, and evaluating the curative effect by using technologies such as image and pathology; and finally, integrating various results to obtain an entrapment material effectiveness analysis conclusion. According to the method, the limitation of traditional single-point testing is broken through, a full-chain evaluation model of structural characteristics-release behavior-biocompatibility-repair efficiency is constructed, and a scientific basis is provided for rational design and clinical transformation of intrauterine adhesion treatment materials.

The invention relates to self-healing hydrogel for preventing intrauterine adhesion and a preparation method and application thereof. The preparation method comprises the following steps: dissolving hyaluronic acid in ultrapure water, then adding EDC and NHS, adjusting the pH value, then adding cysteamine hydrochloride, and after the reaction is finished, dialyzing and freeze-drying to obtain sulfydryl modified hyaluronic acid marked as HASH; dissolving the HASH in ultrapure water, then adding EDC and NHS, adjusting the pH value, then adding dopamine hydrochloride, and after the reaction is finished, dialyzing and freeze-drying to obtain sulfydryl and dopamine modified hyaluronic acid which is marked as DAHASH; carrying out surface sulfhydrylation treatment on the mEXO to obtain an engineered exosome which is marked as SmEXO; and respectively dissolving the DAHASH, the SmEXO and silver nitrate in ultrapure water, and then mixing to obtain an exosome-hydrogel system which is marked as DAHASH (at) SmEXO. The hydrogel for preventing intrauterine adhesion has a self-healing characteristic, is suitable for a special structure of an intrauterine, can be anchored on a wounded surface of a uterus, is continuously adhered to an endometrium for slowly releasing SmEXO, and creates a good condition for long-term repair of the endometrium.

The invention relates to a therapeutic drug, in particular to a construction method and application of a cell factor sustained-release complex combined with stem cells, and the cell factor sustained-release complex can be used for preventing intrauterine adhesion and promoting endometrial repair. The invention aims to provide a construction method and application of a cell factor slow-release complex combined with stem cells, so that the cell factor slow-release complex is effectively applied to repair of intrauterine adhesion and endometrium. According to the technical scheme, the construction method of the cell factor slow-release complex combined with the stem cells comprises the following steps: (1) preparing platelet-rich plasma, activating and enriching platelet-rich plasma cell factors, and adsorbing the released cell factors through hydrogel; (2) preparing a vascular extracellular matrix by using a decellularization technology, wherein the vascular extracellular matrix is used for adsorbing platelet-rich plasma cytokines; (3) acquiring umbilical cord mesenchymal stem cells; constructing an umbilical cord mesenchymal stem cell / extracellular matrix slow-release complex; and (4) preparing the cell factor slow-release complex combined with the stem cells.

The utility model discloses an absorbable biological stent for preventing intrauterine adhesion in the technical field of intrauterine stents, which comprises a biological absorbable stent, the biological absorbable stent comprises two groups of inclined support rods which are symmetrically arranged, the close ends of the two groups of inclined support rods are connected through a first bending section which is integrally formed, and the two groups of inclined support rods are connected through a second bending section which is integrally formed. Inverted elastic supporting parts are integrally formed at the far ends of the two sets of inclined supporting rods, each elastic supporting part comprises a first supporting rod which is integrally formed and connected with the ends of the inclined supporting rods through a second bent section, and the end, away from the second bent section, of each first supporting rod is integrally formed and connected with a second supporting rod through a third bent section. The vertical distance between the end, connected with the second bent section, of the first supporting rod and the inclined supporting rod is smaller than the vertical distance between the end, connected with the third bent section, of the first supporting rod and the inclined supporting rod and the vertical distance between the end, connected with the third bent section, of the first supporting rod and the inclined supporting rod. And the balloon made of the plastic silica gel material needs to be taken out after being used, so that secondary injury is easily caused to the patient.

The invention relates to the technical field of biomedicine, and provides an endometrial repair stent and a preparation method thereof.The endometrial repair stent is prepared by taking a flaky nanofiber composite membrane as a substrate, combining the flaky nanofiber composite membrane with modified hyaluronic acid, then conducting dynamic crosslinking with carboxymethyl chitosan and then conducting compounding with poly N-isopropylacrylamide; the mass ratio of the modified hyaluronic acid to the carboxymethyl chitosan to the poly (N-isopropylacrylamide) is (1-9): (1-9): (1-90); the thickness of the nanofiber composite membrane is 0.1-0.4 mm, and the nanofiber composite membrane can be applied to treatment of intrauterine adhesion. According to the endometrial repair stent, the nanofiber composite membrane and the temperature-sensitive hydrogel are combined, a uterine cavity is fully paved by utilizing the fluidity of the hydrogel, and the three-dimensional structure of the nanofiber can be used for orderly inducing a wound surface, so that the adhesion and proliferation of cells are facilitated, scar formation is reduced, the repair effect is ensured, and regeneration is promoted. The two parts are combined, so that the plasticity of the whole structure is enhanced, the supporting performance is improved, the indwelling time in the uterine cavity is long, and the double effects of repairing and isolating are fully achieved.

A preparation method for a curcumin-loaded recombinant collagen hydrogel and a use of the curcumin-loaded recombinant collagen hydrogel in treatment of intrauterine adhesion. Homogenized curcumin is introduced, so that the curcumin changes from fat-soluble to water-soluble and can be better absorbed by a human body, thereby enhancing the bioavailability. Meanwhile, by combining with a crosslinking agent at a specific concentration, crosslinking between collagen is promoted, so that the recombinant collagen can act on a lesion site for a long time, the loss of the recombinant collagen in delivery is reduced, and the therapeutic effect of the recombinant collagen is improved. The prepared hydrogel effectively solves the technical problems of severe loss of recombinant collagen and unsatisfactory treatment effect when recombinant collagen is directly and locally applied to an intrauterine adhesion site, significantly improves the treatment effect, and has a high clinical value in preventing tissue adhesion.

The invention relates to a pharmaceutical composition for treating uterine adhesion and application thereof. The kidney reinforcing and miscarriage preventing pills are used for improving endometrial blood perfusion and increasing the endometrial thickness, the kidney reinforcing and miscarriage preventing pills, aspirin and estrogen play a synergistic treatment role, the postoperative re-adhesion rate is remarkably reduced through combined treatment, and the fertility prognosis of a patient is improved; and the molecular mechanism of a TGF-beta1 pathway and EMT is deeply researched, and by inhibiting excessive activation of the TGF-beta1 signaling pathway, reducing endometrial fibrosis, regulating an epithelial-mesenchymal transition (EMT) process and promoting regeneration of endometrial epithelial cells, endometrial repair is further promoted, multi-target treatment is realized, and the treatment effect is remarkable. A brand-new and efficient treatment scheme is provided for treatment of intrauterine adhesion, an individualized treatment scheme can be formulated according to the specific conditions of patients, the treatment accuracy and effectiveness are improved, and important clinical significance and application value are achieved.

The invention relates to the technical field of medical care information processing, and discloses a traditional Chinese medicine prescription efficacy evaluation system for treating intrauterine adhesion, comprising: a data preprocessing module for acquiring endometrial dynamic response gray time sequence characteristic data before and after a subject takes a prescription; the phase analysis module is used for determining a phase deviation distribution coefficient according to the phase angle distribution of the gray scale data of the reference pixel and the response pixel; the frequency domain inversion module is used for constructing an energy transfer gain spectrum by calculating a power ratio and identifying a characteristic resonant frequency; the evaluation index output module is used for generating an evaluation index for evaluating the viscoelasticity improvement degree of the endometrial tissue according to the deviation value of the characteristic resonant frequency in combination with the variation of the phase deviation distribution coefficient. And the quantitative accuracy of evaluation is improved.

The invention belongs to the field of medicines, and particularly provides application of mesenchymal stem cells in a medicine for treating thin endometrium and uterine cavity adhesion. The invention further provides a PDGF-BB monoclonal antibody, and the antibody can be specifically combined with PDGF-BB so as to inhibit corresponding protein activity. After the monoclonal antibody is used independently or in combination with stem cells, the effects of effectively promoting gland growth and reducing the fibrosis degree can be achieved, which shows that the monoclonal antibody can be used independently or in combination with the stem cells for treating thin endometrium and uterine cavity adhesion, and the application prospect is wide.

The invention relates to an anti-adhesion uterine cavity support, belongs to the technical field of uterine cavity supports, and provides an anti-adhesion uterine cavity support in order to solve the problems that the size of an existing uterine cavity support cannot be adjusted, pain is caused to a patient when the existing uterine cavity support is used, and a wound in a uterine cavity cannot be wiped and cleaned due to the fact that a wiping device is not arranged. Comprising a balloon, a connecting rod, a multidirectional supporting frame and a wiping device, the balloon and the connecting rod are communicated with each other, the multidirectional supporting frame is arranged in the balloon and the connecting rod, so that the balloon is small in size when placed into the body of a patient, and after placed into the body of the patient, the balloon is expanded to prevent intrauterine adhesion; therefore, wounds on the wall of the uterine cavity are wiped and cleaned, and inflammation and adhesion are further reduced.

The invention relates to the technical field of modernization of traditional Chinese medicines, in particular to a nano-vesicle, a preparation method and application of the nano-vesicle in preparation of an endometrial injury repairing medicine. The preparation method comprises the following steps: taking a periplaneta americana ethanol extracting solution, and adding a buffer solution to prepare diluted homogenate; performing differential centrifugal treatment on the homogenate; filtering the supernatant obtained by the differential centrifugation treatment, and then centrifuging for 60-120 minutes by 100000g of the supernatant to obtain a precipitate; and re-suspending the precipitate to obtain the periplaneta americana ethanol extract source nano-vesicles. The periplaneta americana ethanol extract is used as a starting material to enrich the nano-vesicles for the first time, the combined process of differential centrifugation, membrane filtration and ultracentrifugation is adopted, the obtained nano-vesicles can be applied to the field of endometrial injury repair, especially treatment of gynecological diseases such as intrauterine adhesion and infertility with thin intima, and the blank in the technical field is filled.

The utility model belongs to the technical field of intrauterine adhesion prevention devices, and particularly relates to a degradable supporting diaphragm device for preventing and treating intrauterine adhesion. The technology comprises a degradable diaphragm, the diaphragm is of an inverted pear-shaped structure and is hollow, two supporting rods which are arranged left and right are arranged in the diaphragm, two connecting rods which are arranged left and right are arranged between the two supporting rods, one ends of the two connecting rods are hinged together through a mounting block, and the other ends of the two connecting rods are hinged to the mounting block. The other ends of the two connecting rods are hinged to the ends, corresponding to the connecting rods, of the supporting rods. Fixing blocks are arranged at the bottoms of the supporting rods, and the other ends of the supporting rods are hinged to the fixing blocks; a driving assembly used for driving the connecting rod and the supporting rod to conduct telescopic deformation is arranged in the diaphragm. The problem that in the prior art, a diaphragm is inconvenient to move into the uterus is solved.

The invention provides a split type uterine cavity anti-adhesion device, and belongs to the technical field of intrauterine postoperative anti-adhesion treatment devices. The device comprises two uterine cavity supports which are independently arranged relatively, each uterine cavity support has a compressed state and an expanded state, each uterine cavity support in the expanded state is of a three-dimensional cage-shaped structure with the top closed, each uterine cavity support is used for supporting a part of a uterine cavity, each uterine cavity support has a plurality of models, and the models of the uterine cavity supports are different. The adaptive model is selected according to the actual size of the asymmetric uterine cavity or the special-shaped uterine cavity with the diaphragm, so that the two uterine cavity brackets are adaptive to the whole uterine cavity in shape after being automatically expanded; the device further comprises a conveying system, and the conveying system is used for loading the uterine cavity support in the contracted state and conveying the uterine cavity support to the target position of the uterine cavity so as to complete release. The intrauterine adhesion prevention device is mainly used for solving the technical problems that in the prior art, a device for preventing intrauterine adhesion is narrow in application range and poor in treatment effect.

The invention belongs to the technical field of biomedical materials, and particularly relates to a temperature-sensitive estradiol hydrogel composition, a preparation method and application. According to the composition, a temperature-sensitive responsive network is constructed by introducing a PCL-PEG-PCL triblock copolymer, modified chitosan, a penetration enhancer and a buffer regulation system, so that rapid gelling, controlled-release drug delivery and good retention under a body temperature condition are realized. Through systematic contrast experiments with a plurality of contrast samples, evaluation is carried out from four aspects of gelling performance, drug release behavior, degradation residual rate and adhesiveness, and results show that the composition in a preferable ratio has the comprehensive advantages of good gel stability, long drug release period, good biodegradability, lasting uterine cavity adhesion and the like; good post-operation anti-adhesion and local drug delivery application prospects are realized.

The invention provides a uterine cavity treatment device, and belongs to the technical field of uterine cavity postoperative continuous treatment devices. The treatment device comprises a uterine cavity support and a conveying system, the uterine cavity support comprises a supporting framework and a medicine carrying covering film, a main body of the supporting framework is of a big-end-up three-dimensional cage-shaped structure, angle-shaped protrusions are symmetrically arranged on the left side and the right side of the far end of the supporting framework, and the angle-shaped protrusions obliquely extend upwards with the center of the uterine cavity support as the reference; an arc-shaped structure is formed, so that the whole shape of the supporting skeleton is matched with the uterus shape after the supporting skeleton is automatically expanded; the medicine carrying covering film is at least arranged on the outer side of the supporting framework; a hormone sustained-release drug is arranged in the drug-loading covering membrane and is used for promoting repair of endometrium; the conveying system is used for loading the uterine cavity stent in a contracted state and conveying the uterine cavity stent to a target position to complete release; the technical problem that in the prior art, the effect of treating intrauterine adhesion after an intrauterine operation is poor is mainly solved.

The invention discloses a convenient sampling device for endometrium detection of an intrauterine adhesion patient, and relates to the technical field of medical instruments. The aspirator is arranged in a round hole in the center of the mounting plate in a penetrating manner, a plurality of arc-shaped supporting plates are arranged on one side of the mounting plate at equal angles, and the arc-shaped supporting plates are connected with the mounting plate through supporting mechanisms; the connecting plates are symmetrically arranged on the two sides of the supporting plate front and back, and the upper sides of the connecting plates are connected with the mounting plate through angle adjusting mechanisms; the lifting mechanism is arranged in the supporting plate, the lifting mechanism is connected with the connecting plate, a sliding block is fixed to the lower side of the supporting plate, and the sliding block is arranged in a sliding groove in the bottom plate in a sliding and moving mode; the gear motor is embedded and fixed in one side of the bottom plate, a push screw rod is fixed on an output shaft of the gear motor, the push screw rod is screwed in the bottom plate through a bearing, and the push screw rod is screwed with the sliding block through a thread; the sampling angle and height can be automatically adjusted to adapt to different body positions, the arc-shaped supporting plate softly expands the uterine cavity, injuries are reduced, and the sampling safety and accuracy are improved.

The invention belongs to the field of biological medicine, and particularly relates to injectable expansion composite hydrogel and a preparation method and application thereof.The preparation method comprises the steps that chitosan is dissolved in an acetic acid solution, and a uniform chitosan solution is obtained; the preparation method comprises the following steps: sequentially adding a calcium chloride solution and an MXene dispersion liquid into a chitosan solution, and uniformly mixing to obtain a solution A; preparing a sodium alginate solution, adding sodium bicarbonate into the sodium alginate solution, and uniformly mixing to obtain a solution B; and mixing the solution A and the solution B, and expanding through a cross-linking reaction to form the injectable expanded composite hydrogel. The hydrogel can support the uterine cavity form through the expansion characteristic, and intrauterine adhesion is prevented.

The invention relates to composite hydrogel for promoting endometrial regeneration and resisting fibrosis as well as a preparation method and application of the composite hydrogel. The composite hydrogel consists of a deciduous endometrial decellularized extracellular matrix pre-gel solution and anti-fibrosis drug loaded polymer sustained-release microspheres dispersed in the deciduous endometrial decellularized extracellular matrix pre-gel solution, the material can be gelated in situ at body temperature and has a shear thinning characteristic. According to the invention, dECM with'decidualization energization 'is combined with an anti-fibrosis drug sustained release system to synergistically solve the two problems of insufficient endometrial regeneration and excessive fibrosis, and experiments prove that the dECM can significantly promote functional endometrial regeneration and restore fertility, and has important application value in drugs or medical instruments for treating intrauterine adhesion.

The invention discloses a magnetic / ultrasonic response microcapsule for repairing endometrial injury and a preparation method of the magnetic / ultrasonic response microcapsule. The method is based on an electronic injection micro-fluidic chip, monodisperse liquid drops with uniform size are generated, the monodisperse liquid drops are dropped into collected liquid, and meanwhile, the microcapsule with a core-shell structure is formed through ultraviolet irradiation and crosslinking. According to the core-shell structure microcapsule prepared by the invention, accurate adjustment of the core-shell size and performance can be realized by changing preparation conditions (internal phase and external phase flow rates and the proportion of components in the external phase), the preparation method is simple and easy to implement, the requirements on reaction conditions are low, and the size of the microcapsule can be easily and accurately adjusted; the microcapsule has magnetic field and ultrasonic response, and can effectively repair damaged endometrium to prevent intrauterine adhesion.

The invention relates to a construction method of a bionic endometrial organ, which comprises the following steps: mixing three primary cells to obtain a mixed cell precipitate, and adding the mixed cell precipitate into a bionic organ culture medium; precipitating and resuspending the mixed cells to obtain a cell suspension; and adding Matrigel, and transferring into a constant-temperature cell culture box for culturing, so as to obtain the bionic endometrial organ. The regeneration promoting effect of the bionic endometrial organ provided by the invention is obviously better than that of transplanted pure stromal cells and pure epithelial organ groups, and the endometrial organ of the bionic cells has a huge application value in the aspect of tissue regeneration. According to the construction method of the intrauterine adhesion disease model, a fibrosis inducing factor TGF beta 1 is added into a bionic endometrial organ, immunofluorescent staining of a fibrosis marker alpha SMA is carried out, and the intrauterine adhesion disease model is obtained. By reproducing the pathological change of the intrauterine adhesion inner membrane, the model can be used as an intrauterine adhesion pathological model for disease pathological research or drug screening research.

The invention discloses a preparation method of an exosome-enriched silkworm chrysalis chitosan magnetic microsphere and application of the exosome-enriched silkworm chrysalis chitosan magnetic microsphere in treatment of intrauterine adhesion, the method is based on a droplet microfluidic technology, chitosan suspension of magnetic nanoparticles is prepared and is uniformly distributed through high-speed oscillation, high-voltage direct current is used for realizing dispersion of droplets, and the magnetic microspheres are prepared. And injecting the dispersed liquid drops into an alkaline solution from a proper height, and curing into balls. A large number of in-vivo and in-vitro experiments prove that the synthesized silkworm chrysalis chitosan magnetic microspheres have good biocompatibility and directional movement capacity, meanwhile, the drug loading and releasing performance of the microspheres is stable, the safety meets the in-vivo injection requirement, and a new thought is provided for treatment of intrauterine adhesion.

The application discloses a method for analyzing the effectiveness of a drug loading material for treating intrauterine adhesion, comprising: performing multi-aspect research on the drug loading material and generating an effectiveness analysis result. In terms of performance characterization, the material morphology and pores are analyzed by using microscopic and specific surface area technologies, the drug and carrier compatibility is verified, and the drug physical state is detected; in vitro analysis covers drug release kinetics, and the release amount is measured in a simulated environment, and a curve is fitted; biological compatibility evaluation is cell co-culture with a material leaching liquor, and cell activity is detected; in vivo, the drug loading material is implanted in an animal model, and imaging and pathological technologies are used to evaluate the curative effect; and finally, the effectiveness analysis conclusion of the drug loading material is obtained by comprehensively analyzing the results. The method breaks through the limitation of traditional single-point testing, constructs a whole-chain evaluation model of "structural characteristics-release behavior-biological compatibility-repair efficiency", and provides a scientific basis for rational design and clinical transformation of intrauterine adhesion treatment materials.

The invention provides a combined marker for predicting intrauterine adhesion after abortion curettage, a kit and application, and belongs to the technical field of biological detection. The method comprises the following steps: prospectively collecting macrophages of decidua of a subject, wherein the subject is in Damp; the method comprises the following steps of: selecting a non-IUA group and an IUA group according to B ultrasonic follow-up visit results of two months after operation, and analyzing gene differential expression of macrophages of the two groups of subjects, difference of intrauterine microbiota and change of serum metabolites to finally obtain a method capable of accurately predicting intrauterine adhesion after abortion curettage operation. According to the method, the model efficiency of predicting intrauterine adhesion after abortion curettage is as high as 97.8%, and the method has the advantages of being low in cost and simple and rapid in detection method and has a large application prospect.

The invention belongs to the technical field of cell biology and regenerative medicine, and particularly relates to a pig endometrial mesenchymal stem cell separation culture method and application, separation is carried out from uterus tissue of a large animal, namely, a pig, the steps are simple, the cell obtaining amount is large, the cultured endometrial mesenchymal stem cells have phenotypes of CD34-and CD11b-, and CD44 + and CD105 +, and the endometrial mesenchymal stem cells can be used for separating and culturing the endometrial mesenchymal stem cells. In vitro induced differentiation into bone cells, adipocytes and chondrocytes, during culture, collagen in extracellular matrix is decomposed, DNA is degraded, cell agglutination is reduced and effective digestion of tissues is promoted jointly through combined use of type II collagenase and DNase I, and a high-quality single-cell suspension can be obtained for subsequent centrifugation and culture; cells with intrauterine adhesion, skin and blood vessel injury and the like can be repaired, the repairing method is simple and easy to implement, regeneration and repairing can be performed by compositely implanting the material and a biological material into a focus part, and a new way is provided for cell transplantation treatment.

The application discloses high-adhesion modified mesenchymal stem cells and application thereof in preparation of a medicine for treating intrauterine adhesion. The modified mesenchymal stem cells are genetically engineered to overexpress integrin alpha 2 subunit and / or integrin alpha 5 subunit, thereby enhancing the adhesion capacity to the extracellular matrix (collagen and fibronectin) of the endometrium. In vitro experiments show that the initial adhesion rate, liquid flushing resistance and anoikis resistance of the modified MSCs are significantly better than those of common MSCs. When used in combination with the "dynamic culture and drug delivery device for intrauterine adhesion treatment" applied by the inventor on the same day, the retention rate of the modified MSCs in the simulated intrauterine dynamic culture environment is significantly improved. Animal experiments show that the modified MSCs in combination with the device can significantly promote endometrial regeneration, inhibit fibrosis and improve the pregnancy rate. The application solves the core bottleneck of low cell retention rate and short survival time in the MSC transplantation treatment of intrauterine adhesion by enhancing the adhesion capacity of the MSCs, and forms a complete treatment scheme in cooperation with the device, thereby having a good clinical application prospect.

The present invention discloses an insulin-like growth factor-1-linker polypeptide-collagen, and the linker polypeptide has an amino acid sequence as shown in SEQ ID No.1. The N-terminus of the linker polypeptide designed and synthesized by the present invention simulates the IGF-1 receptor structure, can be combined with IGF-1, and the C-terminus of the linker polypeptide is rich in lysine residues, which can be covalently cross-linked to collagen by transglutaminase, prolonging the attachment time of IGF-1 in the endometrium, and effectively promoting endometrial thickening and endometrial damage repair. A protein solution for promoting endometrial damage repair provided by the present invention can form a stable repair microenvironment on the surface of the endometrium, promote the recovery of the number of endometrial glands with intrauterine adhesions, reduce the proportion of fibrosis, and promote the thickening of thin endometrium, with the advantages of high tissue compatibility, simple preparation, obvious improvement effect, no potential toxic effects on cells, and good clinical application prospects.

The invention discloses a temperature-sensitive hydrogel, a preparation method thereof and an application of the temperature-sensitive hydrogel in preparation of a medicine for treating intrauterine adhesion, and belongs to the technical field of medicines. The temperature-sensitive hydrogel is a buffer solution containing chondroitin sulfate and poloxamer. The thermo-sensitive hydrogel provided by the invention can be used for effectively treating intrauterine adhesion, and has very important clinical application value.