Preparation method and application of collagen scaffold composite bone marrow-derived mesenchymal stem cells (BMSCs)

Abstract

The invention relates to a preparation method and application of collagen scaffold composite bone marrow-derived mesenchymal stem cells (BMSCs). The preparation method comprises the steps of preparing single cell suspension after trypsinizing the BMSCs, uniformly dropwise adding the single cell suspension to a collagen scaffold, putting the collagen scaffold into an incubator to be cultured, and adding an L-DMEM complete medium to continue culture, thus obtaining the collagen scaffold composite BMSCs. The preparation method has the advantages that the following defects are overcome: endometria are seriously injured as intrauterine adhesion is mechanically separated by adopting hysteroscopic surgery, intrauterine devices or anti-adhesion materials are put after the surgery and estrogens are given after the surgery to promote intima growth; the problem of intima scars can not be solved; functional intima repair can not be achieved; adhesion is very easy to happen again. As active ingredients for treating serious endometrium injury, the BMSCs are convenient to obtain, secrete growth factors to improve the local microenvironment and immunoregulation, have good biocompatibility, degradability and safety, promote scarred endometrium repair and increase the intima thickness and local blood vessel density.

Description

technical field [0001] The invention belongs to the field of obstetrics and gynecology, and relates to a preparation method and application of collagen scaffold composite bone marrow mesenchymal stem cells. Background technique [0002] When the endometrium is severely damaged, the functional repair of the endometrium is obstructed, replaced by connective tissue hyperplasia and repair, fibrosis of the anterior and posterior walls of the uterus, and scar formation. Clinically, the main manifestations are intrauterine adhesions, amenorrhea, and uterine infertility. There is no effective treatment. [0003] Prior to the present invention, the treatment for severe endometrial injury was as follows: [0004] 1. The common clinical method is to mechanically separate intrauterine adhesions through hysteroscopic surgery, place intrauterine devices or anti-adhesion materials (mainly hyaluronic acid, Intercoat gel) after surgery, and give estrogen after surgery to promote intrauterin...

AI Technical Summary

Problems solved by technology

However, severe damage to the endometrium cannot solve the problem of endometrial scarring, cannot achieve functional repair of the endometrium, and is very prone to re-adhesion

[0005] 2. Recently, a small sample study reported that after the separation of intrauterine adhesions, human fresh amniotic membrane was used to cover the wound. This method cannot induce the functional repair of the scar endometrium itself, and the amniotic membrane still has risks such as immune inflammation and induced infection.

[0006] 3. Infusion of granulocyte colony-stimulating factor into the uterine cavity is reported to increase the thickness of the endometrium in some patients with thin endometrium. However, this method cannot retain granulocyte colony-stimulating factor in the local area, the action time is short, and the local curative effect is poor. The effect of promoting the growth of scar intima

However, BMSCs are difficult to localize in the injured area after local injection, which affects the curative effect.

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