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Collagen dermis material for promoting endometrium repairing and preparation method of collagen dermis material

A collagen and surfactant technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve problems such as endometrial injury, immune inflammation-induced infection, and inability to solve endometrial scar problems, and achieve good biocompatibility resistance, effective barrier effect, low degradability effect

Active Publication Date: 2017-06-23
YANTAI ZHENGHAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although early intervention has a certain effect on uterine adhesions, it seriously damages the endometrium, cannot solve the problem of endometrial scarring, cannot achieve functional repair of the endometrium, and is very prone to re-adhesion
[0005] (2) Recent studies have used human fresh amniotic membranes for postoperative wound repair after separation of intrauterine adhesions. This method cannot induce scar endometrial self-repair, and there may be risks such as immune inflammation and induced infection

Method used

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  • Collagen dermis material for promoting endometrium repairing and preparation method of collagen dermis material
  • Collagen dermis material for promoting endometrium repairing and preparation method of collagen dermis material
  • Collagen dermis material for promoting endometrium repairing and preparation method of collagen dermis material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, preparation of non-modified collagen dermis material

[0063] 1. Collect dermis and subcutaneous tissue layers

[0064] Pigskins that have just been slaughtered in vitro are collected by a special person from a slaughterhouse under standardized management. Try to avoid contact with pollutants. After collection, they are immediately frozen and stored; The dermis and subcutaneous tissue layer with a thickness of 1.0 mm are taken as raw materials.

[0065] 2. Alkali treatment

[0066] Then use 1mol / L NaOH aqueous solution to soak the raw material obtained in the above 1 for 1h, the temperature is 10°C, the weight ratio of 1mol / L NaOH aqueous solution to raw material is 20:1, remove fat and foreign protein, and obtain the material after alkali treatment, which is the corium Tissue matrix (the main component is collagen).

[0067] 3. Disodium hydrogen phosphate-potassium dihydrogen phosphate aqueous solution treatment

[0068] The material after the alkali ...

Embodiment 2

[0079] Embodiment 2, the preparation of modified collagen dermis material

[0080] 1. Collect dermis and subcutaneous tissue layer: same as 1 of embodiment 1.

[0081] 2, alkali treatment processing: same as 2 of embodiment 1.

[0082] 3, disodium hydrogen phosphate-potassium dihydrogen phosphate aqueous solution processing: same as 3 of embodiment 1.

[0083] 4, lipase treatment: same as 4 of embodiment 1.

[0084] 5, Weak base+surfactant treatment: same as 5 of embodiment 1.

[0085] 6. Polypeptide solution treatment: Soak the weak base+surfactant-treated material obtained in the above 5 in the polypeptide solution for 2 hours at a temperature of 20° C. to obtain the polypeptide solution-treated material.

[0086] The weight ratio of the polypeptide solution to the material after treatment with weak base+surfactant is 20:1.

[0087] The mass percent content of the above polypeptide solution is 0.25%.

[0088] The fragment of the above polypeptide is TKKTLRTGSAGSAAGIKVAV...

Embodiment 3

[0090] Embodiment 3, the preparation of modified collagen dermis material

[0091] 1. Collect dermis and subcutaneous tissue layer: same as 1 of embodiment 1.

[0092] 2, alkali treatment processing: same as 2 of embodiment 1.

[0093] 3, disodium hydrogen phosphate-potassium dihydrogen phosphate aqueous solution processing: same as 3 of embodiment 1.

[0094] 4, lipase treatment: same as 4 of embodiment 1.

[0095] 5, Weak base+surfactant treatment: same as 5 of embodiment 1.

[0096] 6. Polypeptide solution treatment: Soak the weak base+surfactant-treated material obtained in the above 5 in the polypeptide solution for 1.5 hours at a temperature of 20° C. to obtain the polypeptide solution-treated material.

[0097] The solute of the polypeptide solution is polypeptide, and the solvent is water.

[0098] The weight ratio of the polypeptide solution to the material after treatment with weak base+surfactant is 20:1.

[0099] The mass percent content of the above polypepti...

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Abstract

The invention discloses a collagen dermis material for promoting endometrium repairing and a preparation method of the collagen dermis material. For the collagen dermis material for promoting endometrium repairing and the preparation method of the collagen dermis material, the preparation method comprises the following step: soaking collagen in a polypeptide solution, thus obtaining the collagen material, wherein the polypeptide comprises a collagen binding domain, an MSCs aggregation promoting domain, and connecting peptide positioned between the collagen binding domain and the MSCs aggregation promoting domain. The experiment proves that the collagen membrane material prepared by adopting the method provided by the invention has good biocompatibility, good degradability, low immunogenicity and the like, and can effectively promote the aggregation and differentiation of human mesenchymal stem cells (hMSCs), the introduced polypeptide modification can increase the binding, aggregation and differentiation of MSCs, and thus an effective barrier function is achieved for intrauterine adhesion.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a collagen dermis material for promoting endometrium repair and a preparation method thereof. Background technique [0002] Intrauterine adhesions, also known as intrauterine adhesions syndrome, are mainly clinically manifested as abnormal menstruation (oligomenorrhea or amenorrhea), periodic abdominal pain and infertility. Even if they are pregnant, they can also cause risks such as repeated miscarriage, premature delivery, placental adhesions, and placenta accreta. . The main cause of intrauterine adhesions is uterine injury. [0003] Currently, surgical options for the repair of endometrial damage are limited. There are: [0004] (1) The general approach is through early intervention. Among them, in addition to mechanical barriers such as intrauterine devices and Foley catheters with balloons, postoperative systemic estrogen therapy, anti-adhesion materials (mainly hyaluronic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/36A61L27/50
CPCA61L27/24A61L27/362A61L27/3679A61L27/3687A61L27/50A61L2430/22A61L2430/40
Inventor 董佳桓闫玉芳王彬郭松
Owner YANTAI ZHENGHAI BIO TECH
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