58 results about "Collagen membrane" patented technology

Provided is a method for preparing a collagen membrane for guided tissue regeneration. By utilizing the electrochemical deposition technology and using collagen as the starting material, the method achieves the electrochemical deposition in a low-temperature environment using a direct-current source, and thus, in a constant-voltage output mode, prepares a collagen membrane of an asymmetric structure (with one dense surface and one loose and porous surface) in one step at a high voltage. Also, by means of asymmetric cross-linking, the membrane may be asymmetrically degraded in vivo, thus promoting the rapid repair of a wound while keeping a stable shielding effect on the wound. The method features ease to operate, efficiency, environment conservation, and good scalability. The obtained collagen membrane possesses excellent mechanical properties and uniform overall performance. The area of the collagen membrane of the asymmetric structure prepared by the preparation method is equal to that of the electrode plate, such that scale-up production can be achieved by using an electrode plate with a greater area, thereby improving productivity.

The invention provides a preparation method of a mineralized collagen scaffold material with a bionic structure, and belongs to the field of biomedical engineering.The preparation method comprises the steps that an amorphous calcium phosphate solution stabilized by polyacrylic acid is prepared, and the molar ratio of Ca to P in the amorphous calcium phosphate solution is 1.67: 1; preparing a collagen solution containing noradrenaline; carrying out isoelectric focusing treatment on the collagen solution under a constant voltage to form a dense membrane, and stretching the dense membrane to generate 120-200% deformation, so as to obtain a collagen membrane with a bionic structure; and soaking the collagen membrane in the amorphous calcium phosphate solution, and then performing freeze drying to obtain the noradrenaline-mineralized collagen scaffold material. The mineralized collagen scaffold material prepared by the method has excellent biocompatibility, osteogenesis inductivity and sufficient mechanical strength, can effectively promote bone tissue defect repair, and has a good application prospect in the field of bone tissue engineering. The invention further provides the mineralized collagen scaffold material with the bionic structure and application of the mineralized collagen scaffold material.

A collagen membrane with an effect of inducing ordered remodeling of tissues, a preparation method therefor, and use thereof, relating to the technical field of surgical supply materials. The preparation method for the collagen membrane comprises the following steps: subjecting a fiber membrane to a heat pressing treatment to obtain the collagen membrane with the effect of inducing ordered remodeling of tissues. The fiber membrane is formed by cross-stacked fibers with a diameter of 0.5-15 μm. The fibers comprise collagen and poly(L-lactide-co-caprolactone). The mass ratio of the collagen to the poly(L-lactide-co-caprolactone) is 1:(1.5-2.5). By combining the collagen, which has good biocompatibility and an effect of promoting tissue repair, with PLCL, which is biodegradable and features excellent mechanical properties and good cell adhesion, the collagen membrane not only has an extracellular matrix-like structure, but also exhibits good structural stability, facilitating the regulation of cell behaviors and tissue regeneration, and effectively promoting the growth, adhesion, and proliferation of host cells, thereby inducing ordered remodeling of tissues.

The invention relates to a collagen-based artificial cornea with a double-layer structure and capable of rapidly epithelizing and a preparation method thereof, and the preparation method comprises the following steps: adding collagen into an acetic acid solution, stirring and dissolving, and adding a plasticizer to obtain a high-concentration collagen solution and a low-concentration collagen solution; carrying out electrochemical deposition on the high-concentration collagen solution to obtain a collagen membrane I; carrying out photo-crosslinking on the collagen membrane I through strong ultraviolet irradiation; performing chemical crosslinking on the first collagen membrane to obtain a compact layer; a low-concentration collagen solution is taken and poured into the deposition tank, the compact layer is immersed, secondary electrochemical deposition is carried out, and a second collagen film is obtained on the compact layer; the second collagen membrane is subjected to secondary photo-crosslinking through weak ultraviolet irradiation, a loose layer is obtained, and the loose layer and the compact layer are combined to form the collagen-based artificial cornea which is of the double-layer structure and can be rapidly epithelized. The collagen-based artificial cornea with the double-layer structure and capable of being epithelialized rapidly has high light transmittance and meets clinical mechanical properties, the light transmittance at 600 nm is higher than 90%, the tensile strength of the collagen-based artificial cornea is higher than 1 MPa, and the elastic modulus of the collagen-based artificial cornea is higher than 10 MPa. The swelling rate is low, the tissue compression of the product on the autologous cornea can be avoided, the mechanical stimulation on the eye tissue is reduced, and the comfort after implantation is improved.

The invention belongs to the technical field of surgical article materials, and particularly relates to a collagen membrane with an effect of inducing ordered remodeling of tissues as well as a preparation method and application of the collagen membrane. The collagen membrane is obtained by performing hot pressing treatment on a fiber membrane; the fiber membrane is formed by crossing and laminating fibers with the diameter of 0.5-15 microns; the fiber comprises collagen and poly (L-lactide-caprolactone), and the mass ratio of the collagen to the poly (L-lactide-caprolactone) is 1: (1.5-2.5). The collagen membrane provided by the invention combines collagen with good biocompatibility and a tissue repair promoting effect with biodegradable PLCL with excellent mechanical properties and good cell adhesion, not only has an extracellular matrix-like structure, but also has good structural stability, is beneficial to regulation of cell behaviors and tissue regeneration, and can be used for preparing the collagen membrane. The growth, adhesion and proliferation of host cells can be effectively promoted, so that ordered remodeling of tissues is induced.

The application provides a kind of electric field period regulation's biomimetic collagen film material and its preparation method and application, it is related to organic polymer compound processing field, also it is related to medical material technical field.Collagen solution is deposited on electrode in the form of electrolysis in this application, and collagen is formed into film, surfactant and ionic strength regulator are added in collagen solution, surfactant stabilizes collagen dispersion, and film is easy to peel off; Moderate ionic strength regulator is compatible with pH value, reduces film-electrode electrostatic adsorption, and enhances film strength; Further, high concentration collagen solution is concentrated by specific hollow fiber ultrafiltration membrane to electrolyte, collagen is formed into uniform film, and it is ensured that collagen film obtained in continuous film forming process is uniform; By periodically switching electrode polarity, negative electric collagen is deposited into film, and positive switching is used to effectively remove film by electrostatic repulsion; Interface regulator and physical auxiliary are combined to realize deposition-film removal continuity; And the obtained collagen film is dense and uniform, and has high strength.

The invention discloses a collagen membrane as well as a preparation method and application thereof, and belongs to the field of cosmetics. The preparation method of the collagen membrane comprises the following steps: pickling cow leather with lime, washing the pickled cow leather with water, then acidifying the cow leather with hydrochloric acid, washing the acidified cow leather with water, and washing away interfibrous substances until the hardness of the cow leather is 1t; the mmol / g is 0.1 to 0.3 mmol / 100g; cutting the obtained cow leather into particles, rolling the particles by a defibrator to obtain collagenous fibers, then adding a gelatinized acetate starch solution and a gelatinized chitosan solution into the collagenous fibers, and uniformly mixing and kneading to obtain collagenous fiber slurry with the water content of 95-97%; and casting the obtained collagen fiber slurry into a membrane, drying at 50-55 DEG C for 2-4 hours, soaking the membrane in a mixed solution of baking soda and glutamine transaminase for 2-3 seconds, drying at 50-55 DEG C, flushing by adopting a sodium thiosulfate solution, and washing to remove salt, thereby obtaining the collagen membrane. According to the preparation method, crosslinking among collagenous fibers is enhanced, and the structural stability of the membrane is improved.

According to the preparation method of the moisturizing wound protecting membrane, the wound protecting membrane is of a double-layer structure and comprises a polyurethane outer membrane and a composite membrane composed of collagen sponge and an animal intestine membrane, and the preparation method of the moisturizing wound protecting membrane comprises the following steps that S1, the polyurethane outer membrane is prepared; s2, preparing a film layer; s3, preparing a collagen layer; s4, freeze-drying and molding the collagen layer and the membrane layer in a mold to obtain a collagen membrane; and S5, bonding the obtained polyurethane outer membrane with a collagen membrane to prepare the double-layer wound protecting membrane. The wound protecting membrane provided by the invention adopts a design of tightly combining three layers, the acellular matrix middle layer can be gradually degraded to inhibit scar hyperplasia, the polyurethane outer membrane provides a physical barrier and promotes cell adhesion and wound healing, and the three-dimensional porous collagen sponge bracket accelerates vascularization and cell ingrowth, so that the wound protecting membrane has a good wound healing effect. A novel decellularization technology is integrally adopted to retain biological activity, and an environment beneficial to skin growth is constructed through a double-layer structure.

The invention provides a preparation method of a water-responsive shrinkage collagen membrane. The preparation method comprises the following steps: providing a cyanidin-coated gallium-indium liquid metal and a collagen solution; mixing the cyanidin-coated gallium-indium liquid metal with a collagen solution, and carrying out vacuum drying to obtain slices; and salting out the slices by using a sodium citrate solution, and stretching to obtain the water-responsive shrinkage collagen membrane. The collagen membrane prepared by the method disclosed by the invention has excellent water response shrinkage performance, can be quickly shrunk under the stimulation of water, and also has excellent mechanical properties, electrical conductivity and oxidation resistance.

The invention discloses an antioxidant and antibacterial collagen repair membrane and a preparation method thereof. The repair membrane is prepared by sequentially carrying out polylysine graft modification and catechinic aldehyde and aminoglycoside antibiotic synchronous covalent grafting on a purified collagen membrane. By controlling parameters such as molecular weight and concentration of polylysine, proportion and loading capacity of functional molecules, reaction conditions and the like, accurate regulation and control of film thickness, function release rate, oxidation resistance and antibacterial performance can be realized. The invention further provides a double-layer composite membrane structure, the rapid antibacterial layer and the long-acting anti-oxidation layer are compounded, and function decoupling and time sequence collaboration are achieved. The repair membrane has excellent oxidation resistance and broad-spectrum antibacterial performance, is good in biocompatibility and adjustable in degradation rate, can be customized according to different clinical requirements, and is suitable for oral wound repair, periodontal guided tissue regeneration, bone tissue induced regeneration, peri-implantitis prevention and treatment and chronic refractory wound treatment.

The application discloses a preparation method of a collagen membrane material which can exist stably in a body for a long time. A dense and transparent collagen membrane with a transparence of more than 95% is formed by using an electric field to control assembly of collagen fibers, the fiber structure is once physically cross-linked and solidified, and further chemical strengthening cross-linking is carried out, the cross-linking strength is improved through different reaction sites, and then the shielding of the in-vivo enzymatic cutting site is carried out. The advantage is that the collagen assembly fiber diameter can be controlled to be 30-50nm by applying high electric field energy, the collagen assembly fiber is densely and orderly arranged, the collagen assembly fiber arrangement is bionic to natural tissue collagen protein fiber arrangement, has high bionics, and can exist stably in a body for a long time.

The invention discloses a method for preparing a collagen fiber hemostatic material capable of rapidly absorbing water, expanding and rebounding by a directed self-assembly method, which comprises the following specific steps: S1, preparing a collagen solution: taking a collagen raw material, stirring and dissolving by using an acetic acid solution, and adding sodium chloride to prepare the collagen solution; s2, electrochemical assembly: carrying out electrochemical self-assembly after carrying out centrifugal treatment on the collagen solution in the step S1 to prepare a collagen membrane material; s3, cross-linking: carrying out cross-linking on the collagen membrane material in the step S2 under an ultraviolet irradiation condition; and S4, freeze-drying: carrying out gradient low-temperature freeze-drying on the collagen membrane material subjected to cross-linking in the step S3 to obtain a finished product. Sodium chloride in the prepared material has a charge shielding effect, reduces the electrification property of collagen and a stacked compact structure of assembled fibers, so that a porous state is formed on the surface of the collagen membrane subjected to electrochemical assembly; and the porous capillary effect is utilized to quickly absorb water.

The invention discloses a method for preparing a wet spinning alignment collagen membrane with high tensile strength, and relates to the technical field of biomedical material preparation, the method comprises the following steps: preparing three layers of solutions, the upper layer and the lower layer are wet spinning buffer solutions (such as a polyethylene glycol solution) with specific concentration and pH, and the middle layer is a collagen solution with specific concentration and pH; injecting the three-layer solution into three-layer flow control equipment, and controlling the flow through a collecting tube; gathering the three layers of solutions at the tail end of equipment and performing wet spinning to form a film; and axially stretching to obtain a finished product. According to the invention, by precisely regulating and controlling a solution system, adopting industrial-grade flow control equipment and flow control, optimizing a directional stretching process and stabilizing a curing environment, the problems of non-uniform film thickness, easy tearing, large mechanical property fluctuation and the like in a traditional process are solved, large-scale production of the collagen film with high tensile strength and directionally arranged molecules is realized, and the production cost is reduced. The method is suitable for artificial blood vessels, bile ducts, skin and other tissue engineering fields.

The present invention relates to a method of producing a collagen membrane that has particular mechanical properties. In particular, the present invention relates to a method A of producing a collagen membrane comprising the steps of (i) isolating a collagen-containing tissue and incubating same in an ethanol solution; (ii) incubating the collagen-containing tissue from step (i) in a first solution comprising an inorganic salt and an anionic surfactant in order to denature non-collagenous proteins contained therein; (iii) incubating the collagen-containing tissue produced in step (ii) in a second solution comprising an inorganic acid until the collagen in said material is denatured; and (iv) incubating the collagen-containing tissue produced in step (iii) in a third solution comprising an inorganic acid with simultaneous mechanical stimulation for sufficient time to enable the collagen bundles in said collagen-containing tissue to align; wherein the mechanical stimulation comprises applying tension cyclically to the collagen-containing tissue.

The present invention discloses a collagen-based artificial cornea with a double-layer structure and the ability to rapidly epithelialize, and a preparation method thereof, comprising the following steps: adding collagen to an acetic acid solution, stirring and dissolving, and adding a plasticizer to obtain a high-concentration collagen solution and a low-concentration collagen solution; electrochemically depositing the high-concentration collagen solution to obtain a first collagen film; photocrosslinking the first collagen film by irradiating the first collagen film with strong ultraviolet light; chemically crosslinking the first collagen film to obtain a compacting layer; pouring the low-concentration collagen solution into a sedimentation tank, immersing the compacting layer, and performing a second crosslinking process. The second electrochemical deposition method obtains a second collagen membrane on the compacting layer; the second collagen membrane is subjected to secondary photocrosslinking by weak ultraviolet irradiation to obtain a loose layer, and the loose layer and the compacting layer are combined to form the double-layer structure and the collagen-based artificial cornea capable of rapid epithelialization. The double-layer structure and the collagen-based artificial cornea capable of rapid epithelialization of the present invention have high light transmittance and mechanical properties that meet clinical requirements: the transmittance at 600nm is greater than 90%, the tensile strength is greater than 1Mpa, and the elastic modulus is greater than 10MPa. The low swelling rate can avoid the product from pressuring the autologous corneal tissue, reduce mechanical stimulation to the eye tissue, and improve comfort after implantation.

The invention belongs to the technical field of wound dressings, and discloses a collagen membrane with a porous structure as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing mannitol and ethanol, heating and stirring to obtain a dissolved solution, standing, cooling, drying in vacuum and sieving to obtain recrystallized mannitol crystals; mixing the recrystallized mannitol crystal and the collagen solution, sequentially carrying out ultrasonic dispersion and vacuum degassing treatment to obtain a blended solution, and sequentially carrying out pre-freeze-drying treatment, vacuum freeze-drying treatment, washing and vacuum drying to obtain a collagen membrane precursor; and immersing the collagen membrane precursor in a cross-linking agent solution, carrying out a cross-linking reaction, carrying out water washing to obtain a cross-linked collagen membrane, immersing the collagen membrane in an antibacterial agent solution, carrying out adsorption, and carrying out washing and vacuum drying to obtain the collagen membrane with a porous structure. The collagen membrane obtained by the invention has the characteristics of being porous (good in moisture absorption and air permeability), antibacterial, excellent in mechanical property and good in biocompatibility.

A glaucoma drainage tube and its manufacturing process are disclosed, relating to the field of ophthalmic medical device technology. The tube is manufactured using a drainage tube manufacturing device, which includes a negative electrode, a positive electrode, and a power source. The negative electrode comprises a middle section and a terminal section, with the outer surface of the terminal section being trumpet-shaped. The negative electrode consists of two interlocking parts. The distance between the central axis of the middle section and the positive electrode is 0.3–2 cm. The positive and negative terminals of the power source are connected to the negative and positive electrodes, respectively. The manufacturing process includes the following steps: providing an acidic electrolyte; placing the working electrode and the positive electrode in the electrolyte for an electrochemical reaction to form a collagen film on the circumferential surface of the working electrode, obtaining a preliminary product; cross-linking the preliminary product; and then extracting the two parts of the negative electrode from within the collagen film towards the terminal section, respectively, to obtain the glaucoma drainage tube. This method can produce a glaucoma drainage tube with trumpet-shaped ends, improving the problem of easy displacement of existing glaucoma drainage tubes after implantation into the eye.

This invention belongs to the field of medical materials, specifically relating to a patterned bio-barrier membrane with self-adhesive function and its preparation method. The preparation method includes: dissolving the adhesive matrix in a buffer solution, then adding reactive amino acids and a stabilizer to obtain a first mixture; adding ethanol and a hydrophobic agent to the first mixture, and stirring under heating conditions to obtain a second mixture; adding a thickener, a calcium supplement, and a reinforcing agent to the second mixture, continuing stirring, and then adding a protein precipitation reagent to precipitate the mixture, obtaining a hydrogel; covering a collagen membrane with a patterned porous mold, and adding the hydrogel to allow it to flow into the pores and solidify. This invention achieves precise adhesive loading through patterned design, combining advantages such as simple operation, reliable fixation, good biocompatibility, and high osteogenic efficiency, effectively solving the barrier membrane fixation problem in GBR technology, and is suitable for bone defect treatment in dental implant prosthesis restoration.

The invention belongs to the technical field of medical materials, and particularly relates to a biomimetic mineralized collagen membrane, a preparation method thereof and application of the biomimetic mineralized collagen membrane in preparation of medical materials. The invention provides a biomimetic mineralized collagen membrane. The biomimetic mineralized collagen membrane comprises collagen fibrils, the inorganic matter is distributed on the outer surface of the collagen fibril and in the collagen fibril along the long axis of the collagen fibril; the inorganic matter is hydroxyapatite; the mass percentage of inorganic matters in the biomimetic mineralized collagen membrane is 6-62%. The biomimetic mineralized collagen membrane provided by the invention can induce mesenchymal stem cells to be differentiated into fibrocartilage cells in vitro, can induce fibrocartilage tissue regeneration in vivo, and can promote tendon bone tissue healing, and the maximum mechanics can be recovered to 67% of normal. Therefore, the biomimetic mineralized collagen membrane provided by the invention is used for a biomimetic tendon bone tissue scaffold as a medical material for repairing tendon bone injury, and has great market potential.

The invention discloses an electrostatic spinning instant collagen membrane and a preparation method thereof. The electrostatic spinning instant collagen membrane comprises the following raw materials in percentage by mass: 40%-80% of a natural high-molecular compound, 5%-40% of collagen, 5%-20% of a film-forming agent and 5%-40% of an instant solvent, the electrostatic spinning instant collagen membrane disclosed by the invention has a rapid dissolving property, and compared with a traditional collagen membrane with a dissolving speed of 2-10 minutes, the collagen membrane disclosed by the invention can be rapidly dissolved within 1-30 seconds; a fluorine-free solvent system is used in the preparation process, a chemical cross-linking agent is not needed, and harmful residues are avoided; meanwhile, under the combined action of the raw materials, the mechanical property of the collagen membrane is improved.

The application discloses a collagen membrane and a preparation method and application thereof, and belongs to the cosmetic field. The preparation method of the collagen membrane comprises the following steps: using lime to pickle a cowhide, washing the cowhide, then using hydrochloric acid to acidify the cowhide, washing the cowhide, washing away the interstitial substance, until the hardness of the cowhide is less than 0.1-0.3 mmol / 100g; cutting the obtained cowhide into pieces, crushing the pieces by using a fiber removing machine, obtaining collagen fibers, then adding a gelatinized acetic acid ester starch solution and a chitosan solution into the collagen fibers, uniformly mixing and rubbing the collagen fibers, and obtaining a collagen fiber slurry with a water content of 95-97%; casting the obtained collagen fiber slurry into a film, drying the film at 50-55 DEG C. for 2-4h, then soaking the film in a mixed solution of baking soda and glutamine transaminase for 2-3s, drying the film at 50-55 DEG C., then washing the film by using a sodium thiosulfate solution, and washing away the salt, so that the collagen membrane is obtained. The preparation method enhances the crosslinking between the collagen fibers, and improves the structural stability of the film.

The invention relates to the technical field of collagen-containing products, in particular to collagen membrane cloth as well as a preparation method and application thereof. The invention relates to collagen membrane cloth, which is prepared by taking modified collagen fibers as raw materials, opening, carding and lapping to prepare a fiber web, and then performing spunlace processing, the modified collagen fiber is prepared from a sea cucumber collagen-sea cucumber peptide-plant polyphenol covalent complex, and the plant polyphenol is one or a mixture of two of Chinese herbaceous peony polyphenol and chaenomeles speciosa polyphenol. According to the collagen membrane cloth, collagen is extracted from marine animal tissues, and the collagen membrane cloth has excellent antioxidant, antibacterial, anti-inflammatory and analgesic effects.

The present application relates to the technical field of biological materials, in particular to a double-layer guided tissue regeneration membrane and a preparation method thereof, so as to improve the comprehensive performance of the double-layer guided tissue regeneration membrane. In the preparation, collagen type I is dissolved in acid to prepare a collagen type I solution; then, an alkali is added to adjust the pH value, so as to prepare collagen type I fibers; then, the collagen type I fibers are processed and molded, and gradient freeze-drying is performed, so as to prepare a collagen type I membrane; then, the collagen type I membrane is crosslinked with a crosslinking agent, so as to prepare the double-layer guided tissue regeneration membrane; wherein, the concentration of the collagen type I solution is 0.5-1.5%, and the pH value is 7-9. The double-layer guided tissue regeneration membrane prepared by the above preparation method is composed of a dense layer and a loose layer formed on the surface of the dense layer, the thickness of the dense layer is 0.2-0.8 mm, and the thickness of the loose layer is 0.4-0.8 mm. The tensile strength is greater than or equal to 28 N, the suture strength is greater than or equal to 4.7 N, and the porosity is greater than or equal to 65.8%, which effectively solves the problems of insufficient mechanical performance and low biological safety of the existing double-layer guided tissue regeneration membrane. The preparation process is simple, green and large-scale.

The invention relates to an autologous active biological membrane for promoting wound repair in breast augmentation and application of the autologous active biological membrane, belongs to the technical field of medical materials, and is suitable for skin wound repair scenes. The invention provides an active biological membrane loaded with ICA-TGF-DEF fusion protein, the ICA-TGF-DEF fusion protein is selected from an amino acid sequence as shown in any one of SEQ ID NO: 4-6, the biological membrane takes collagen as a membrane matrix, and then the ICA-TGF-DEF fusion protein as shown in any one of SEQ ID NO: 4-6 and the collagen membrane matrix are subjected to crosslinking treatment to obtain the active biological membrane loaded with the ICA-TGF-DEF fusion protein. The self-active biological membrane for promoting wound repair in breast augmentation is obtained through a biological membrane preparation method, in the aspect of physical performance, the biological membrane has high expansion rate, porosity and high protein loading capacity, in the aspect of biological activity, the biological membrane can remarkably enhance cell attachment and proliferation, and an antibacterial wound microenvironment can be created to promote tissue repair; the dressing is especially suitable for wound repair after breast augmentation, and can accelerate tissue healing and reduce infection and fibrosis risks.

The invention relates to the field of skull repair, and provides a fully-degradable multi-layer composite structure skull repair net and a preparation method thereof.According to the skull repair net, a compact collagen film layer is prepared on the outer surface through solvent volatilization of a solution, and a porous electrostatic spinning collagen film layer is prepared on the inner surface through an electrostatic spinning technology; the middle layer is prepared into a degradable alloy net structure through a laser cutting technology, and the middle layer is bonded through a 1% (w / v) collagen aqueous solution to form the skull repair net with the multi-layer composite structure. One side of the compact layer is used for shielding fibroblast migration, and one side of the porous microfiber is used for promoting cell adhesion proliferation and osteogenesis and blood vessel formation. The middle alloy net-shaped structure provides mechanical support, slowly degrades and releases Zn < 2 + > and promotes bone repair, in addition, the hollow parallel arc design can simultaneously realize out-of-plane deformation in the direction perpendicular to the net-shaped structure and stretching or compression deformation under the action of in-plane stress, large-area falling in the degradation process is avoided, and the secondary operation risk is avoided. Good application prospects are realized.

The invention belongs to the technical field of biomedical materials, and particularly relates to a clinical ready-to-use autologous adipose tissue-derived matrix collagen membrane and application thereof. The clinical ready-to-use autologous adipose tissue-derived matrix collagen membrane provided by the invention is mainly composed of autologous adipose tissue-derived collagen, and contains a plurality of key adhesion proteins, glycosaminoglycans, growth factors and other bioactive components. The preparation method comprises the following steps: by taking autologous adipose tissue as a raw material, breaking cells by a mechanical method, repeatedly cleaning to remove cell components such as cell debris and oil drops, and mechanically pressing through a mold to form a diaphragm with uniform thickness, regular shape and rough surface. The membrane effectively overcomes the limitation of the existing acellular matrix biological membrane, can load various bioactive substances to enhance the function, can be stored at low temperature for a long time without influencing the repair effect, and greatly enhances the convenience of subsequent use.

A meniscus tear repair sling device has a collagen membrane having a first end portion and a second end portion. The first end portion is configured to pass under a tear in a meniscus and wrap over and above the tear and extend past the meniscus and overlay the second end portion thereby enveloping a portion of the meniscus with a tear and forming the sling device. The first end portion and the second end portion form exposed overlying tails extending external of a knee joint. The tails are configured to be affixed to a portion of a tibia. The tails when tensioned close the tear by providing a lateral strain on the torn meniscus when the tails are affixed to a tibia adjacent the knee joint.

This invention provides an artificial blood vessel with anti-bleeding function. The vessel has a hollow tubular structure and comprises, from the inside out, a collagen membrane layer, a fabric composite layer, and a sprayed layer. The fabric composite layer is a composite fabric of polyester fibers and core-spun yarn, with the surface of the core-spun yarn containing a suitable amount of yarn hairs. The collagen membrane layer of this artificial blood vessel gives it low immunogenicity and good biocompatibility, and also facilitates cell endothelialization. The fabric composite layer utilizes its surface hairs to give the blood vessel excellent anti-bleeding properties and enhance the compliance of the artificial blood vessel. The sprayed layer can cover the hairs of the core-spun yarn, preventing excessive contact with blood and blood coagulation, and also improving the strength of the blood vessel. The artificial blood vessel of this invention not only has excellent anti-bleeding properties but also meets the requirements of compliance, compatibility, and strength for artificial blood vessels, which is of great significance for solving the bleeding problem of existing artificial blood vessels and promoting the application of artificial blood vessels.

The application provides a collagen drying film forming method, which comprises the following steps: a preparation step, a dilution step, a defoaming step, a coating step, a pressing step, a drying step, a heat cross-linking step, a water covering step and a freeze drying step. The collagen drying film forming method provided by the application can improve the drying efficiency and product yield while retaining the integrity of the triple helix of collagen, and the dried collagen film has good mechanical properties and good flatness.

The invention discloses a preparation system and method of a collagen membrane corneal contact lens. The preparation system comprises an electrochemical deposition system, a voltage control system and an in-situ optical measurement feedback system, the electrochemical deposition system is used for generating the corneal contact lens and comprises an electrolytic tank, a cathode substrate and an anode substrate, collagen electrolyte is contained in the electrolytic tank, and the cathode substrate comprises a layer of substrate material matched with the concave shape of the corneal contact lens and a layer of transparent conductive film formed on the substrate material in a vapor deposition mode; the anode substrate is electrode units which are arranged in an array, the electrode units are suspended right above the cathode substrate, and the collagen electrolyte is deposited in an area where a continuous electric field formed by the electrode units is located to form a convex surface of the corneal contact lens. According to the preparation system and method, the electrochemical deposition technology and electric field accurate control are innovatively combined, high controllability of the lens shape and the optical performance is achieved, and wide application prospects are achieved.