209 results about "Collagen membrane" patented technology

A composite product is disclosed as a collagen support comprising at least one porous collagen layer covered on at least one side with an essentially compact collagen membrane consisting either of a collagen film prepared by drying a collagen gel, preferably in air or a gaseous fluid, or of a very highly compressed collagen sponge. At least one of the two layers, i.e. the porous layer and the essentially compact membrane, may comprise normal, genetically modified or malignant living cells originating particularly from young or elderly subjects. This composite product is used as a collagen support for the manufacture of artificial skin intended especially for performing in vitro tests on the efficacy of potentially active substances or for reconstructing damaged areas of skin in vivo.

The invention relates to a guided tissue regeneration membrane, which comprises a compact coating and a loose coating, wherein the compact coating is composed of collagen-I or collagen-I composite hyaluronic acid; and the loose coating is composed of collagen-I or collagen-I composite calcium salt. A preparation method of the guided tissue regeneration membrane comprises the following steps of: preparing a collagen solution or a collagen and hyaluronic acid mixed solution by the use of acetic acid; injecting the solution into a self-made die, and drying it in the air to prepare the compact coating; injecting the collagen solution or a mixed liquor of the collagen solution and calcium salt onto the compact coating, and uniformly spreading; precooling the die at ultralow temperature, followed by freeze-drying to prepare the guided membrane with the compact coating and loose coating structure; carrying out vacuum high-temperature crosslinking and chemical crosslinking; and finally cleaning. The regeneration membrane has good histocompatibility and mechanical strength, low antigenicity and strong guided tissue regeneration capability. Its degradation rate in vivo is 3-8 months. The method is easy to operate, is suitable for automatic and continuous large-scale production, and solves the problems of large size of collagen membrane and material uniformity during industrial production.

Collagen membranes are formed from porcine rinds (i.e., pig skins) for us in a variety of applications and, most preferably, for wrapping food products, such as hams and the like. First, after removing skins from the porcine, the skins are promptly frozen. In later processing, the rinds are thawed and then enzymatically defatted. Then, a quick alkalinic hydrolyzation is performed on the rinds. Then, an acidic hydrolyzation is performed on the rinds. The rinds are then ground into a gel-like fluid mass. Finally, the fluid mass is extruded, sheeted and dried into a collagen membrane. The collagen membrane produced can be, in preferred embodiments, used for wrapping food products, such as hams.

The invention relates to a double-layer composite regeneration film and a making method thereof. The regeneration film comprises a compact layer and a loose layer, the compact layer is formed through compounding a degradable high-molecular fiber web with a collagen film, or with collagen and nano-silver particles, or with collagen and a medicine having a treatment effect, and the loose layer is formed by treating one or more selected from chitosan, silk fibroins, keratoprotein and collagens as a matrix material. The preparation method mainly comprises a step of preparing the fiber strengthened compact layer, a step of preparing the loose layer, and a step of preparing a guide film through compounding. The regeneration film has the advantages of good biocompatibility and mechanical performances, biodegradability, low immune rejection response, simple operation and the like, and can be widely used for restoring tissues, especially bone tissues and periodontal tissues.

The present invention provides a preparation method of high-strength bionic collagen membrane. The preparation method is by conducting gradient dialysis process on collagen to rearrange collagen molecules into an ordered structure, and endow the molecules with more consistent and more extensive collagen fiber orientation, thus improving the mechanical properties of the material. The collagen membrane prepared by using the method has similar appearance, structure and performance to natural dura and amnion of mammals, and good rehydration, and is free to bend and fold after water absorption; the bionic collagen membrane with high tensile strength can be stitched by using suture and can be used in clinical guided tissue regeneration or repair of various tissues.

The invention relates to a method of in vitro testing of the efficacy of a potentially active substance comprising monitoring the effect of said potentially active substance on an artificial skin, comprising a composite product forming a collagen support comprising at least one porous collagen layer covered on at least one side with a collagen membrane component selected from the group consisting of a collagen membrane prepared by compression of a collagen sponge at a pressure of at least about 50 bar and of a collagen membrane comprising a collagen film prepared by drying a collagen gel separately from the porous collagen layer, thereby providing a reliable method for finding new potentially active substances.

A subantral membrane elevator comprising an inflatable balloon and an over-formed membrane (e.g., collagen membrane), which is resorbable into the body, and a method of using the same in bone augmentation surgery, such as alveolar bone augmentation preparatory to implantation of a dental implant.

The invention discloses a method for producing a non-fishy smell collagen membrane by fish processing leftovers, such as fishbone, fish skin, fish scale, fish muscle and the like. The technical process comprises the following steps: removing non collagen, degreasing, removing fishy odor and deashing, stirring and the like to the fish processing leftovers, such as the fishbone, the fishskin, the fish scale, the fish muscle and the like; adding plasticizer in the collagen acquired by hot water extraction and centrifugal separation, stirring, defoaming, drying and the like to prepare the collagen membrane. The invention utilizes fish processing leftovers as main raw materials, and develops a new way for the comprehensive utilization and the product development of the fish processing leftovers; the prepared collagen membrane has good mechanical strength and transparency and no fishy odor, can replace parts of plastic packaging films, can be eaten together with the packaged food, and has good effects on reducing the waste emission of aquatic product processing enterprises, reducing the use of the plastic packaging film in food, and protecting the environment.

The invention relates to a method of in vitro testing of the efficacy of a potentially active substance comprising monitoring the effect of said potentially active substance on an artificial skin, comprising a composite product forming a collagen support comprising at least one porous collagen layer covered on at least one side with a collagen membrane component selected from the group consisting of a collagen membrane prepared by compression of a collagen sponge at a pressure of at least about 50 bar and of a collagen membrane comprising a collagen film prepared by drying a collagen gel separately from the porous collagen layer, thereby providing a reliable method for finding new potentially active substances.

A subantral membrane elevator comprising an inflatable balloon and an over-formed membrane (e.g., collagen membrane), which is resorbable into the body, and a method of using the same in bone augmentation surgery, such as alveolar bone augmentation preparatory to implantation of a dental implant.

In various embodiments, a collagen product is provided that is derived from an animal, the collagen product comprises precipitated collagen that is substantially pure. In various embodiments, the collagen is obtained from a marine animal and does not contain prions or viruses. In various embodiments, the collagen can be made or incorporated into collagen films, collagen membranes, cosmetic collagen masks, collagen sponges, gelatin, hemostasis sponges, lyophilized foams, collagen injections, artificial skins and dura, bones, cartilage, screws, shafts, stems, or tube guides.

The present invention relates to a method for preparing a cell-derived extracellular matrix membrane, more particularly, to a method for preparing a chondrocyte-derived ECM membrane, the method comprising the steps of forming a suitable thickness of ECM membrane by culturing chondrocytes derived from animal cartilage at a high concentration in vitro, and drying it after decellularization process. The cell-derived ECM membrane scaffold according to the present invention is composed of extracellular matrix secreted by chondrocytes so that the membrane has excellent biocompatibility as well as an immune-previlage effect specific to cartilage. Since the membrane also has a suitable compressive strength, it can be used to replace periosteum for cartilage regeneration or artificial collagen membrane and used as dura mater transplant material, a natural ECM membrane for treating skin loss, materials for cell transplantation and a growth factor delivery vehicle.

The invention relates to novel collagen materials and, specifically, collagen membranes, tubes and threads. Said materials combine enhanced properties of resilience and strength. The invention also relates to a method for preparing collagen materials using acid fibrous collagen comprising coagulation and, optionally, cross-linking of the collagen in the presence of ammonia gas.

The invention discloses a growth factor-loading silk fibroin / collagen bracket material and a preparation method thereof. The material is prepared from the raw materials of collagens, growth factors, silk fibroin, polylysine (PLL) and heparin sodium. A loose layer fibroin / collagen film and a compact layer fibroin / collagen film form a double-layered structure, and growth factor-loading PLL-heparin sodium nanoparticles are compounded in the middle of the double-layered structure. The preparation method comprises the following steps: preparing the loose layer fibroin / collagen film and the compact layer fibroin / collagen film; preparing the growth factor-loading nanoparticles, compounding the fibroin / collagen film and the nanoparticles and the like. The growth factor-loading silk fibroin / collagen bracket material disclosed by the invention plays a directional differential slow release role on the loaded growth factors and can fully maintain the activity of the growth factors and effectively prolong the action time of the growth factors, so that the material is a good guided tissue regeneration material for prosthodontic treatment of impaired and damaged tissues.

The invention discloses a biomembrane for ocular surface restoration and a preparation method thereof. The biomembrane is a compact collagen membrane structure, comprises collagen I, collagen III and various active factors as main materials, has thickness of 0.01-0.05mm, tensile strength of 30-60MPa and stitching tearing force of 5-15N, and has effects of inhibiting scar and inflammation. Compared with the existing product, the biomembrane retains various natural components and a structure of amnion, has the same treatment effects and mechanical strength similar to that of fresh amnion and avoids virus hidden trouble of the biomembrane. The biomembrane is suitable for ocular surface burn and conjunctiva injury restoration, has good functions of promoting epithelium generation, resisting inflammation and inhibiting scar and has good treatment effects.

The invention relates to a preparation method of a multi-layer absorbable biological membrane. The method comprises the steps of preparing a chondroitin sulfate acetic acid solution and a collagen acetic acid swelling solution; adding the chondroitin sulfate acetic acid solution to the collagen acetic acid swelling solution to obtain collagen-chondroitin sulfate slurry; carrying out first vacuum freeze-drying on a collagen chondroitin sulfate solution to obtain a collagen membrane; pressing the collagen membrane into a compact collagen membrane; pouring the collagen chondroitin sulfate solution into a freeze-drying plate paved with the compact collagen membrane or directly adhering a compact collagen layer to the collagen membrane through the collagen-chondroitin sulfate slurry, carrying out second vacuum freeze-drying to obtain a compound collagen membrane; crosslinking the compound collagen membrane; and sterilizing the crosslinked compound collagen membrane to obtain the multi-layer absorbable biological membrane. The multi-layer absorbable biological membrane formed by a loose layer and the compact layer is prepared from high-purity I-type collagen as a raw material; and the multi-layer absorbable biological membrane has good biocompatibility, and is nontoxic and free of antigen reaction.

A biocompatible, self-supporting, curved, collagen membrane adapted to be secured by bone tacks or bone screws over exposed bone at a desired bone graft site in the alveolar ridge of a patient such that the membrane defines a space having a predetermined height and width over the exposed bone, in which the membrane maintains its structural integrity for at least 4 months after implantation at the bone graft site and then naturally breaks down and is resorbed by the patient's body, a method of making such a membrane, and a method of using such a membrane for vertical augmentation of the alveolar ridge of the patient.

The invention discloses a metal polyphenol collagen membrane material as well as a preparation method and application thereof. The metal polyphenol collagen membrane material is a hydrogel material and comprises collagen and a nano complex loaded on a porous structure of the collagen, and the nano complex is obtained by chelating metal ions and polyphenol. The metal polyphenol collagen membrane material has good pH responsiveness, has antibacterial and hemostatic effects at the same time under response conditions, has an excellent skin wound repairing effect, and can be applied to skin wound repairing materials.

The invention relates to a guided tissue regeneration membrane and a preparation method thereof. The method comprises: a pretreatment step, i.e. collecting a mammalian peritoneum, conducting flowing water washing, hair removal and preliminary degreasing for standby use; an antigen removal treatment step, i.e. carrying out further degreasing on the standby peritoneum by means of an enzyme treatment process, an alkali treatment process, an acid treatment process and a hypertonic treatment process, and removing immunogenicity; a collagen fiber contraction treatment step, i.e. carrying out dehydration, non-hydrophilic substance removal and washing treatment on the peritoneum subjected to the antigen removal treatment so as to contract the collagen fibers of the peritoneum; and a drying step, spreading the contracted peritoneum on a culture dish surface to make the compact layer of the peritoneum contact the culture dish, rapidly transferring the peritoneum into a refrigerator at a temperature ranging from -60 to -80DEG C to undergo pre-freezing for 1-8h, then freeze-drying the peritoneums in a freeze dryer for 24-36h, thus obtaining a collagen membrane able to guide tissue regeneration. The guided membrane provided by the invention has a compact layer and a loose layer, also has good biocompatibility, hydrophilicity and bone induction ability, and has a long in-vivo degradation time.

The invention discloses a preparation method of a collagen film for guided tissue regeneration. The method comprises the following steps of dissolving collagen in an acid solution, adjusting a pH (potential of hydrogen) value of an obtained solution to 5-9, continuing to conduct rapid shearing and stirring on the obtained solution, obtaining a collagen emulsion, centrifuging the obtained collagen emulsion, collecting the collagen film on the solution, allowing the obtained collagen film to be subjected to mold forming, freeze-drying, using a physical or / and chemical method for crosslinking, and obtaining the collagen film for the guided tissue regeneration. The obtained collagen film has excellent mechanical property and controlled degradation property, has a gradient pore structure, is suitable for transmission of nutrient substances and adsorption and multiplication of cells, and can better guide repair of defect tissues; the preparation technology is simple; the cost is lower; and the large-scale production is facilitated.

The invention discloses a novel bilayer membrane for guiding bone regeneration and a preparation method thereof. The bilayer membrane comprises a double-layer membrane prepared by type I collagen andpolycaprolactone and a nano-scale zeolite imidazole ester skeleton material coated on the double-layer membrane. 8 coating, the preparation method comprises the following steps: S1) preparing a two-layer film; S2) drying and disinfecting; S3) coating a nano-scale zeolite imidazole ester skeleton material-8 coating. The invention solves the technical defect that the traditional collagen membrane has poor mechanical properties and collapses easily, and the degradation in the body is too fast, has good biocompatibility, and is beneficial to cell growth and soft tissue healing.

The invention discloses a BMP loaded silk fibroin / collagen scaffold material. The material is prepared with collagen, BMP, silk fibroin and PLGA as raw materials; a preparation method comprises the steps of preparation of a loose-layer silk fibroin / collagen membrane and a dense-layer silk fibroin / collagen membrane, preparation of BMP loaded PLGA microspheres, composite of the silk fibroin / collagen membranes and the microspheres and the like. A scaffold is a tissue engineering scaffold having no toxic or side effect on bodies and having good biocompatibility and tissue repair capacity. Based on combination of respective advantages of good biological compatibility, biological degradation and the like of two different natural biological macromolecules of silk fibroin and collagen, the BMP is introduced to the scaffold, a directional controlled-release slow-release technology is adopted, the BMP is allowed to be released persistently, the problems of relatively short in-vivo half-life period of the BMP, growth factor directional introduction local defect repairing and the like are solved effectively, and full playing of functions are prolonged.

The invention discloses a preparation method of a composite crosslinked type I collagen membrane. The method includes the steps of: firstly subjecting type I collagen to dissolution stirring in an acid solution in a reaction kettle for 12-24h, then adding a defoaming agent into the solution, conducting stirring for 20-60min, then pumping the solution into a storage tank, and conducting static defoaming; conducting inspection to ensure that defoaming reaches the requirement, then pumping the solution into a film casting machine, carrying out film casting, then performing drying, dipping the film into a first cross-linking agent solution or placing the film under cross-linking conditions for 12-24h, and conducting cleaning, then dipping the film into a second cross-linking agent solution or placing the film under cross-linking conditions for 12-24h, and then performing cleaning and drying; and finally, conducting forming processing, packaging and irradiation, thus obtaining the composite crosslinked type I collagen membrane. The membrane material has good mechanical properties, hydrophilicity, antimicrobial activity and biological compatibility, the degradation rate is controllable and can reach long degradation time, also the preparation process is simple, the equipment is simple, the raw materials are cheap and easily available, and the composite crosslinked type I collagen membrane can be widely used as medical wound dressings, tissue engineering scaffold materials and the like in the medical field.

The invention discloses a preparation method of a vinyl modified collagen film. Firstly, a vinyl collagen solution is prepared; in the presence of a certain concentration of photoinitiator, the vinyl collagen solution is placed under ultraviolet light, and after several hours Irradiation cross-links to form a film, which is insoluble in water and has high strength. The invention carries out vinyl modification on the collagen, and after the ultraviolet cross-linking is triggered by an initiator, the vinyl collagen has a dense network structure. The mechanical properties of the collagen film are improved, and the problem of insufficient mechanical properties of the collagen film is successfully solved. The process is simple, requires less raw materials, and has good economic benefits and social application value.

The invention provides a method for preparing an absorbable and antibacterial guided tissue regeneration membrane for a bone-like structure, and belongs to the field of biological medical materials. The method comprises the following steps: firstly, preparing a cell free bovine pericardial collagen fiber membrane by adopting a repeated freeze-thaw method and a surface active agent TritonX-100, or preparing a demineralized cell free collagen fiber membrane of a cattle lamellar bone by using a method of EDTA decalcification by using the surfactant TritonX-100; treating by using sodium trimetaphosphate to obtain a phosphorylated collagen fiber membrane; with the assistance of a direct-current electric field, mineralizing in agar hydrogel containing calcium and phosphate, thereby obtaining a mineralized collagen membrane with a bone-like structure with certain hardness; and finally soaking the mineralized collagen membrane into a minocyline solution, and preparing an antibacterial bone-like structured composite membrane structure by virtue of the characteristics of combined minocyline and hydroxyapatite. The guided tissue regeneration membrane provided by the invention has a bone-like structure, is capable of maintaining a bone defect space, is good in operation forming property and antibacterial property, and can be applied to clinical practice for bone defect remediation and reconstruction in periodontal departments, dental implant mediation and occlusalf surfaces.

The invention relates to a preparation method for an antioxidation collagen active membrane containing a plant extract, and belongs to the field of edible membrane blending modification. The antioxidation collagen active membrane contains a plant extract, collagen and glycerol. The preparation method comprises the following steps of: dissolving the collagen and the glycerol into distilled water to prepare a collagen aqueous solution according to proportion; adding the plant extract into the distilled water to prepare a solution; adding a certain amount of the solution into the collagen aqueous solution, uniformly stirring, and performing ultrasonic degassing; and weighing and paving on an organic glass plate, and drying to prepare the membrane. Compared with a pure collagen membrane, the prepared active protein membrane has the advantages that the antioxidation performance is improved obviously due to adding of the extract, and effects of high ultraviolet resistance and certain antibacterial performance are achieved; and moreover, the protein membrane has high mechanical strength and moisture and gas resistance and is degradable, so that effects of protecting foods and reducing environmental pollution are achieved.

The invention discloses a collagen membrane medical dressing, which is prepared in the way that: 1swung dash10 parts of sodium carboxymethylcellulose and 15swung dash150 parts of deionized water are mixed in a reaction bulb into the transparent thick state; 0.05swung dash0.5 part of resina draconis and 0.2swung dash2 parts of polyvidon are mixed and prepared into solid dispersoid through a liquation method; 0.05swung dash0.5 part of collagen and 0.1swung dash1 part of starch are added into 1swung dash5 parts of glutaraldehyde solution, are stirred to dissolve at 20 swung dash30 DEG C, then are mixed with the prepared sodium carboxymethylcellulose solution, and are stirred at 30swung dash50 DEG C, so a pasty mixed liquor is prepared for standby application; and 0.05swung dash0.5 part of pseudo-ginseng and 0.25swung dash2.5 parts of solid dispersion are added into the pasty mixed liquor, and are uniformly mixed at 30swung dash50 DEG C, the pH of the pasty mixed liquor is controlled between 6swung dash8, the pasty mixed liquor is poured into a die to form, and is dried in a drying oven, and the collagen membrane medical dressing product is produced only after the dyestripping.

The invention discloses a collagen dermis material for promoting endometrium repairing and a preparation method of the collagen dermis material. For the collagen dermis material for promoting endometrium repairing and the preparation method of the collagen dermis material, the preparation method comprises the following step: soaking collagen in a polypeptide solution, thus obtaining the collagen material, wherein the polypeptide comprises a collagen binding domain, an MSCs aggregation promoting domain, and connecting peptide positioned between the collagen binding domain and the MSCs aggregation promoting domain. The experiment proves that the collagen membrane material prepared by adopting the method provided by the invention has good biocompatibility, good degradability, low immunogenicity and the like, and can effectively promote the aggregation and differentiation of human mesenchymal stem cells (hMSCs), the introduced polypeptide modification can increase the binding, aggregation and differentiation of MSCs, and thus an effective barrier function is achieved for intrauterine adhesion.

The invention relates to a preparation method of a collagen membrane and the collagen membrane thereof, which belongs to the biology field. The collagen membrane has the compact performance and appropriate degradability, and can effectively protect an acellular dermal matrix layer and avoid wound infection. The membrane comparison overcomes the defects of rarefaction and improper usage as a protection layer for the corium layer of tissue engineering skin in prior art. The preparation method of the collagen membrane comprises the following steps: preparing a collagen solution, wherein pH value of the collagen solution is 68-; gelling the collagen solution to obtain collagen block; concussing and crosslinking the collagen block under effect of a cross-linking agent, and then cleaning; and pressing the cleaned collagen block to obtain the collagen membrane.