15 results about "Collagen membrane" patented technology

The invention discloses an antioxidant and antibacterial collagen repair membrane and a preparation method thereof. The repair membrane is prepared by sequentially carrying out polylysine graft modification and catechinic aldehyde and aminoglycoside antibiotic synchronous covalent grafting on a purified collagen membrane. By controlling parameters such as molecular weight and concentration of polylysine, proportion and loading capacity of functional molecules, reaction conditions and the like, accurate regulation and control of film thickness, function release rate, oxidation resistance and antibacterial performance can be realized. The invention further provides a double-layer composite membrane structure, the rapid antibacterial layer and the long-acting anti-oxidation layer are compounded, and function decoupling and time sequence collaboration are achieved. The repair membrane has excellent oxidation resistance and broad-spectrum antibacterial performance, is good in biocompatibility and adjustable in degradation rate, can be customized according to different clinical requirements, and is suitable for oral wound repair, periodontal guided tissue regeneration, bone tissue induced regeneration, peri-implantitis prevention and treatment and chronic refractory wound treatment.

The application discloses a preparation method of a collagen membrane material which can exist stably in a body for a long time. A dense and transparent collagen membrane with a transparence of more than 95% is formed by using an electric field to control assembly of collagen fibers, the fiber structure is once physically cross-linked and solidified, and further chemical strengthening cross-linking is carried out, the cross-linking strength is improved through different reaction sites, and then the shielding of the in-vivo enzymatic cutting site is carried out. The advantage is that the collagen assembly fiber diameter can be controlled to be 30-50nm by applying high electric field energy, the collagen assembly fiber is densely and orderly arranged, the collagen assembly fiber arrangement is bionic to natural tissue collagen protein fiber arrangement, has high bionics, and can exist stably in a body for a long time.

The invention belongs to the technical field of wound dressings, and discloses a collagen membrane with a porous structure as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing mannitol and ethanol, heating and stirring to obtain a dissolved solution, standing, cooling, drying in vacuum and sieving to obtain recrystallized mannitol crystals; mixing the recrystallized mannitol crystal and the collagen solution, sequentially carrying out ultrasonic dispersion and vacuum degassing treatment to obtain a blended solution, and sequentially carrying out pre-freeze-drying treatment, vacuum freeze-drying treatment, washing and vacuum drying to obtain a collagen membrane precursor; and immersing the collagen membrane precursor in a cross-linking agent solution, carrying out a cross-linking reaction, carrying out water washing to obtain a cross-linked collagen membrane, immersing the collagen membrane in an antibacterial agent solution, carrying out adsorption, and carrying out washing and vacuum drying to obtain the collagen membrane with a porous structure. The collagen membrane obtained by the invention has the characteristics of being porous (good in moisture absorption and air permeability), antibacterial, excellent in mechanical property and good in biocompatibility.

A glaucoma drainage tube and its manufacturing process are disclosed, relating to the field of ophthalmic medical device technology. The tube is manufactured using a drainage tube manufacturing device, which includes a negative electrode, a positive electrode, and a power source. The negative electrode comprises a middle section and a terminal section, with the outer surface of the terminal section being trumpet-shaped. The negative electrode consists of two interlocking parts. The distance between the central axis of the middle section and the positive electrode is 0.3–2 cm. The positive and negative terminals of the power source are connected to the negative and positive electrodes, respectively. The manufacturing process includes the following steps: providing an acidic electrolyte; placing the working electrode and the positive electrode in the electrolyte for an electrochemical reaction to form a collagen film on the circumferential surface of the working electrode, obtaining a preliminary product; cross-linking the preliminary product; and then extracting the two parts of the negative electrode from within the collagen film towards the terminal section, respectively, to obtain the glaucoma drainage tube. This method can produce a glaucoma drainage tube with trumpet-shaped ends, improving the problem of easy displacement of existing glaucoma drainage tubes after implantation into the eye.

This invention belongs to the field of medical materials, specifically relating to a patterned bio-barrier membrane with self-adhesive function and its preparation method. The preparation method includes: dissolving the adhesive matrix in a buffer solution, then adding reactive amino acids and a stabilizer to obtain a first mixture; adding ethanol and a hydrophobic agent to the first mixture, and stirring under heating conditions to obtain a second mixture; adding a thickener, a calcium supplement, and a reinforcing agent to the second mixture, continuing stirring, and then adding a protein precipitation reagent to precipitate the mixture, obtaining a hydrogel; covering a collagen membrane with a patterned porous mold, and adding the hydrogel to allow it to flow into the pores and solidify. This invention achieves precise adhesive loading through patterned design, combining advantages such as simple operation, reliable fixation, good biocompatibility, and high osteogenic efficiency, effectively solving the barrier membrane fixation problem in GBR technology, and is suitable for bone defect treatment in dental implant prosthesis restoration.

The invention discloses an electrostatic spinning instant collagen membrane and a preparation method thereof. The electrostatic spinning instant collagen membrane comprises the following raw materials in percentage by mass: 40%-80% of a natural high-molecular compound, 5%-40% of collagen, 5%-20% of a film-forming agent and 5%-40% of an instant solvent, the electrostatic spinning instant collagen membrane disclosed by the invention has a rapid dissolving property, and compared with a traditional collagen membrane with a dissolving speed of 2-10 minutes, the collagen membrane disclosed by the invention can be rapidly dissolved within 1-30 seconds; a fluorine-free solvent system is used in the preparation process, a chemical cross-linking agent is not needed, and harmful residues are avoided; meanwhile, under the combined action of the raw materials, the mechanical property of the collagen membrane is improved.

The present application relates to the technical field of biological materials, in particular to a double-layer guided tissue regeneration membrane and a preparation method thereof, so as to improve the comprehensive performance of the double-layer guided tissue regeneration membrane. In the preparation, collagen type I is dissolved in acid to prepare a collagen type I solution; then, an alkali is added to adjust the pH value, so as to prepare collagen type I fibers; then, the collagen type I fibers are processed and molded, and gradient freeze-drying is performed, so as to prepare a collagen type I membrane; then, the collagen type I membrane is crosslinked with a crosslinking agent, so as to prepare the double-layer guided tissue regeneration membrane; wherein, the concentration of the collagen type I solution is 0.5-1.5%, and the pH value is 7-9. The double-layer guided tissue regeneration membrane prepared by the above preparation method is composed of a dense layer and a loose layer formed on the surface of the dense layer, the thickness of the dense layer is 0.2-0.8 mm, and the thickness of the loose layer is 0.4-0.8 mm. The tensile strength is greater than or equal to 28 N, the suture strength is greater than or equal to 4.7 N, and the porosity is greater than or equal to 65.8%, which effectively solves the problems of insufficient mechanical performance and low biological safety of the existing double-layer guided tissue regeneration membrane. The preparation process is simple, green and large-scale.

A meniscus tear repair sling device has a collagen membrane having a first end portion and a second end portion. The first end portion is configured to pass under a tear in a meniscus and wrap over and above the tear and extend past the meniscus and overlay the second end portion thereby enveloping a portion of the meniscus with a tear and forming the sling device. The first end portion and the second end portion form exposed overlying tails extending external of a knee joint. The tails are configured to be affixed to a portion of a tibia. The tails when tensioned close the tear by providing a lateral strain on the torn meniscus when the tails are affixed to a tibia adjacent the knee joint.

This invention provides an artificial blood vessel with anti-bleeding function. The vessel has a hollow tubular structure and comprises, from the inside out, a collagen membrane layer, a fabric composite layer, and a sprayed layer. The fabric composite layer is a composite fabric of polyester fibers and core-spun yarn, with the surface of the core-spun yarn containing a suitable amount of yarn hairs. The collagen membrane layer of this artificial blood vessel gives it low immunogenicity and good biocompatibility, and also facilitates cell endothelialization. The fabric composite layer utilizes its surface hairs to give the blood vessel excellent anti-bleeding properties and enhance the compliance of the artificial blood vessel. The sprayed layer can cover the hairs of the core-spun yarn, preventing excessive contact with blood and blood coagulation, and also improving the strength of the blood vessel. The artificial blood vessel of this invention not only has excellent anti-bleeding properties but also meets the requirements of compliance, compatibility, and strength for artificial blood vessels, which is of great significance for solving the bleeding problem of existing artificial blood vessels and promoting the application of artificial blood vessels.

The invention discloses a collagen-based dura mater containing a specific microporous layer and a preparation method thereof. The collagen-based dura mater containing the specific microporous layer comprises a first collagen mater layer, a second collagen mater layer and a third collagen mater layer, wherein the first collagen mater layer is suitable for facing the skull side and at least comprises mineralized collagen materials; the first collagen membrane layer is suitable for facing the brain tissue side and at least comprises a second collagen membrane layer compounded by collagen and other materials; the microporous layer is located between the first collagen membrane layer and the second collagen membrane layer and at least comprises collagen, and the range of the pore diameter and the porosity of the microporous layer meets the requirements that the microporous layer is suitable for supporting growth and migration of meningeal cells, is also suitable for preventing leakage of cerebrospinal fluid and invasion of fibroblasts, and is also suitable for ensuring a continuous phase of mechanical support; the pore diameter of the microporous layer is 5-20 [mu] m, the porosity is 60-80%, and the thickness is 20-120 [mu] m. According to the technical scheme, a three-layer composite bionic structure is constructed by introducing the microporous layer with specific pore diameter and porosity between the two collagen membrane layers, so that cross-scale collaborative optimization of mechanical support, biological activity and anti-leakage performance of the dura mater repair material is realized.

The present application provides a use of an exosome-loaded collagen membrane complex in the preparation of a material for cervical repair, and a preparation method of the exosome-loaded collagen membrane complex includes fusion culture of exosomes and a collagen membrane. The exosome-loaded collagen membrane complex can quickly promote epithelial tissue regeneration, thereby preventing cervical restenosis, and the exosome-loaded collagen membrane has a double-layer structure, which is beneficial to the growth of different types of cells.

The invention provides a method for modular efficient assembly of a bionic collagen membrane with D period characteristics based on an LB technology. The method comprises the following main process steps: S1, preparing a collagen solution for LB membrane preparation; s2, preparing a substrate LB film; s3, standing and micro-amplitude pressure circulation are carried out; and S4, modularly assembling the collagen membrane. According to the method, on the premise of not changing a collagen formula and mild environmental conditions, two key process steps of continuously standing after balanced mould pressing and forming a'compression-relaxation 'effect with micro-amplitude pressure circulation are introduced, so that the spreading and rearrangement sufficiency of interface molecules is improved, residual interface stress is released, and the collagen quality is improved. Therefore, the structural integrity and the preparation stability of the membrane layer are remarkably improved, the collagen membrane which has higher membrane forming yield and D-period success rate and keeps biological activity is obtained, the stability of the collagen membrane with the D-period characteristic assembled on the basis of an LB technical path is promoted to step from a sample level to a process level, and a reliable manufacturing basis is provided for subsequent application and amplification.

The present application relates to the technical field of medical and cosmetic raw materials, and particularly relates to a triple helix structure collagen membrane and a preparation method and application thereof. The triple helix structure collagen membrane is prepared by vacuum freeze-drying treatment without involving a chemical cross-linking agent, and has excellent mechanical properties and high production efficiency. The triple helix structure collagen membrane has excellent biocompatibility, so that when the triple helix structure collagen membrane is applied to the preparation of medicines or cosmetics, the triple helix structure collagen membrane cannot cause skin inflammation and other immune responses.

The invention belongs to the field of medical materials, and particularly relates to a patterned biological barrier film with a self-adhesion function and a preparation method thereof. The preparation method comprises the following steps: dissolving an adhesive main body in a buffer solution, and then adding reactive amino acid and a stabilizer to obtain a first mixed solution; adding ethanol and a water repellent agent into the first mixed solution, and stirring under a heating condition to obtain a second mixed solution; adding a thickening agent, a calcium source supplement and a reinforcing agent into the second mixed solution, continuously stirring, and adding a protein precipitation reagent for precipitation to obtain hydrogel; and covering the patterned porous mold with a collagen membrane, adding the hydrogel to enable the hydrogel to flow into the pores for curing, and completing the process. Accurate adhesive loading is achieved through patterning design, the device has the advantages of being easy and convenient to operate, reliable in fixation, good in biocompatibility and high in osteogenesis efficiency, the barrier film fixing problem in the GBR technology is effectively solved, and the device is suitable for bone defect treatment in oral implant denture repair.

The present application relates to a kind of preparation methods of transparent collagen membrane, the method includes the following steps: (a) obtaining animal-derived acellular collagen membrane;(b) at least three times transparentization is carried out to collagen membrane.The present application uses animal-derived material to obtain collagen membrane by decellularization, makes its triple helix structure more close to tissue itself, has good mechanical property and degradation performance.At the same time, the collagen membrane prepared by the method according to the present application is treated by three times transparentization, collagen network structure is uniform, and stable is uniformly transparent color, and the light transmittance of material cannot be influenced by the change of pH, can maintain the beauty of healed tissue while accelerating the process of wound repair healing.