The invention relates to a collagen-based
artificial cornea with a double-layer structure and capable of rapidly epithelizing and a preparation method thereof, and the preparation method comprises the following steps: adding collagen into an
acetic acid solution, stirring and dissolving, and adding a
plasticizer to obtain a high-concentration collagen solution and a low-concentration collagen solution; carrying out electrochemical deposition on the high-concentration collagen solution to obtain a
collagen membrane I; carrying out photo-crosslinking on the
collagen membrane I through strong
ultraviolet irradiation; performing chemical crosslinking on the first
collagen membrane to obtain a compact layer; a low-concentration collagen solution is taken and poured into the deposition tank, the compact layer is immersed, secondary electrochemical deposition is carried out, and a second collagen film is obtained on the compact layer; the second collagen membrane is subjected to secondary photo-crosslinking through weak
ultraviolet irradiation, a loose layer is obtained, and the loose layer and the compact layer are combined to form the collagen-based
artificial cornea which is of the double-layer structure and can be rapidly epithelized. The collagen-based
artificial cornea with the double-layer structure and capable of being epithelialized rapidly has high light
transmittance and meets clinical mechanical properties, the light
transmittance at 600 nm is higher than 90%, the tensile strength of the collagen-based artificial
cornea is higher than 1 MPa, and the
elastic modulus of the collagen-based artificial
cornea is higher than 10 MPa. The swelling rate is low, the
tissue compression of the product on the autologous
cornea can be avoided, the mechanical stimulation on the eye tissue is reduced, and the comfort after implantation is improved.