The invention particularly relates to a 3D printing skull repair titanium mesh containing growth factors and a preparation method thereof.The preparation method comprises the steps that a to-be-repaired head is analyzed, and skull defect part information is obtained; 3D printing is conducted according to the skull defect part information, and a titanium mesh skull model is obtained; adding the titanium mesh skull model into a dopamine solution to obtain a polydopamine microsphere modified titanium mesh skull model; mixing the hydroxyapatite, the microspheres, the collagen solution and the silk fibroin solution to obtain the 3D printing bio-ink; 3D printing bio-ink is printed into a coating to cover the polydopamine microsphere modified titanium mesh skull model, and after-treatment is conducted, the skull repair titanium mesh is obtained. The 3D printing titanium mesh and the guided tissue regeneration layer containing the growth factors are combined, the surface roughness and biological activity of the titanium mesh material can be improved, combination of the material and bones is promoted, adhesion and proliferation of cells are promoted, osteogenesis is induced, and repairing and healing of skull tissue are accelerated.