22 results about "Fibrocartilage" patented technology

The invention discloses a nanofiber functional grading scaffold and a preparation method thereof. The preparation method comprises the following steps: step 1, respectively preparing a PLCL / silk fibroin spinning solution, a PLCL / silk fibroin / MDP1 spinning solution and a PLCL / silk fibroin / hydroxyapatite spinning solution; 2, processing the three spinning solutions into nanofiber yarns through electrostatic spinning; and step 3, by taking the PLCL / silk fibroin nanofiber yarns as warp yarns and taking the three kinds of nanofiber yarns as weft yarns in sequence, weaving on a machine to obtain the stent. In the nanofiber functional grading scaffold, polypeptide MDP1 is loaded in the nanofiber yarn for simulating a fibrous cartilage interface, so that the situation that the mechanical property of the whole structure is influenced by scar hyperplasia caused by excessive inflammation of a tendon-bone interface can be prevented; meanwhile, hydroxyapatite with an excellent osteogenesis promoting effect is loaded in the nanofiber yarn for simulating the bone interface, and the mechanical property of the tendon-bone interface is further enhanced.

The invention relates to the technical field of biomedicine, and provides an efficient separation and culture method of rat meniscus fibrocartilage cells. The yield and activity of the meniscus fibrocartilage cells are improved through a step-by-step enzyme digestion technology, the primary cell adherence time is shortened to 4-6 h by optimizing culture conditions, the breakthrough of sufficiently extracting the meniscus fibrocartilage cells from a single rat is achieved, the use amount of experimental animals is greatly reduced, and the cost is reduced. Meanwhile, it is ensured that the cell activity and proliferation capacity meet the requirements of subsequent passage and functional research, and the method has remarkable advantages in the aspects of ethical compliance, cost control and experimental efficiency.

The invention discloses an application of didymoside in a medicine for promoting postoperative tendon-bone interface fibrous cartilage regeneration. According to the application, an important way for treating the tendon-bone interface injury and preventing the tendon-bone healing disorder is obtained through the research of macrophages on fibrous cartilage tendon-bone interface repair and the influence of didymoside on interface bone formation and structure, fibrous cartilage formation and macrophage polarization regulation, so that the tendon-bone interface injury is further treated.

Provided herein are chondro-promotive nanogels, and multilayer membranes with one layer including a dispersion of the chondro-promotive nanogels, and another layer including encapsulated osteo-promotive agents. The provided materials are particularly useful for guiding fibrocartilaginous interface regeneration in a subject having an injured tendon–bone interface. Also provided are methods of producing the disclosed multilayer membranes, and methods of using the multilayer membranes for repair of tendon–bone interfaces.

The present invention discloses a cationic nanocarrier for penetrating fibrocartilage and its preparation method and application, which belongs to the field of biomedicine technology. The preparation method comprises the following steps: mixing an ethylenediamine solution and a Boc-Lys(Fmoc)-OH solution, adding HATU and HOAt for activation, adding DIPEA for further activation, stirring the reaction, adding glacial ether and standing to obtain a precursor; mixing the precursor and a piperidine solution, adding glacial ether and n-hexane and standing to obtain DGL; reacting DGL in a mixed solution of TFA, DCM and TIS to obtain de-Boc DGL, and reacting the de-Boc DGL with phenylboronic acid in a solvent. The cationic nanocarrier prepared by the present invention can reversibly bind to glycosaminoglycans in cartilage and penetrate the full layer of condylar cartilage of the temporomandibular joint while carrying siRNA to target deep chondrocytes, giving lysosomal escape ability, promoting chondrocyte uptake, and improving siRNA transfection efficiency.

Systems (500, 1000) and methods (1700) for fabricating a soft tissue implant (100, 400). The methods generally involve: receiving implant data representative of the target implant; determining a planned weaving path for forming the soft tissue implant; and communicating the planned weaving path to an output device.

The invention relates to the technical field of biological medicine, in particular to a traditional Chinese medicine composition for promoting regeneration of hyaline cartilage and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-30 parts of prepared rehmannia root, 10-30 parts of astragalus membranaceus, 10-20 parts of Chinese yam, 10-20 parts of cistanche deserticola, 5-15 parts of angelica sinensis, 5-15 parts of corydalis tuber, 5-15 parts of spina date seed and 3-10 parts of safflower carthamus. The traditional Chinese medicine composition has the traditional effects of tonifying kidney and replenishing essence, tonifying qi and activating blood, and activating meridians to stop pain, and is proved to be capable of effectively relieving oxidative stress of a local microenvironment of joint injury and remodeling metabolic homeostasis of mesenchymal stem cells by up-regulating expression of HIF-1alpha and PLK1, so that regeneration of high-quality transparent cartilage is specifically induced, formation of fibrocartilage is inhibited, and the joint injury effect is improved. The composition is used for preventing or treating articular cartilage defects and osteoarthritis.

The invention discloses a bionic intervertebral disc integrated organoid based on 3D printing and a construction method and application thereof, and relates to the field of biomedical engineering. The construction method comprises the following steps that a polymer material is adopted to prepare a crossed net-shaped bionic stent through 3D printing, the crossed net-shaped bionic stent is curled to form a cylindrical fiber ring structure, and the fiber ring bionic stent is obtained; performing mesoderm induction on the pluripotent stem cells, and respectively performing cartilage induction and fibrous cartilage induction to obtain nucleus pulposus-like cartilage organs and fibrous ring-like fibrous cartilage organs; the cartilage-like organ is placed in the center of the fibrous ring bionic scaffold, the fibrous cartilage-like organ is filled in the grid structure of the fibrous ring bionic scaffold, and after matrigel wrapping and incubation curing, the bionic intervertebral disc integrated organ based on 3D printing is obtained through induction culture. The bionic intervertebral disc integrated organ based on 3D printing provided by the invention can effectively reproduce structural characteristics and functional characteristics of a natural intervertebral disc.

The present application belongs to the technical field of polymer materials, and particularly relates to a negative Poisson's ratio swelling and toughening hydrogel material and a preparation method and application thereof. The hydrogel material comprises a fiber skeleton and a hydrogel bonded to the fiber skeleton, and the fiber skeleton has a cosine wave negative Poisson's ratio structure, specifically a periodic structure formed by cosine curves staggered in horizontal and vertical directions. By adjusting the preparation parameters of the cosine wave fiber, the specific surface area of the fiber is large and the swelling effect is obvious, the fiber and the hydrogel form rich covalent grafting, and the cosine wave negative Poisson's ratio structure fiber drives the molecular chains of the hydrogel to be oriented and arranged to form an ordered structure when subjected to tension, so that the strength and toughness of the hydrogel are effectively improved. The prepared negative Poisson's ratio swelling and toughening hydrogel material can be used as a surgical material, such as a replacement or repair of fibrocartilage and / or fibrous connective tissue material.

The application discloses a preparation method of a multi-phase "core-shell" biomimetic tissue engineering support, and constructs a multi-phase "core-shell" in-situ biomimetic support to promote the reconstruction of a rotator cuff tendon-bone interface; the support is made of PCL / PEO biological materials and mixed with a plurality of bioactive factor microspheres, the support fiber is a "core-shell" structure, the "shell" contains SDF-1 chitosan slow-release microspheres, the "inner core" is a bone tissue layer, a fibrocartilage layer and a tendon layer, the layers are continuously printed, the spatial distribution of various factors is fine and controllable, the tendon-bone interface is reconstructed in a biomimetic manner, the sequential controllable release of SDF-1 in the outer layer and various differentiation induction factors in the inner core is realized by using the "core-shell" structure fiber, the endogenous MSCs recruitment and the ordered directional differentiation of each layer are promoted, and the application develops an electrohydrodynamic coaxial 3D printing technology on the basis of the electrohydrodynamic printing technology, and constructs a multi-phase "core-shell" in-situ biomimetic support to promote the reconstruction of the rotator cuff tendon-bone interface.

The invention relates to the technical field of oral disease animal model construction methods, in particular to a construction method of a traumatic temporomandibular ankylosis animal model. The construction method comprises the following steps: selecting an anterior auricular region on one side of an experimental subject, avoiding temporal superficial artery and vein, passively separating from front to back along a zygomatic arch to a joint capsule, exposing a mandibular condylar process neck and part of the zygomatic arch, and separating a condylar process after opening the joint capsule; the outer side attachment of the joint disc is separated through the joint gap, the peripheral attachment of the joint disc is cut off after the joint disc is exposed, and part or all of the joint disc is removed; the joint space is exposed through horizontal blunt separation of the joint capsule, part of fibrous cartilage on the condylar process head is removed, and fossa articular cartilage is damaged; after being sober, the animals eat soft food, and the traumatic temporomandibular joint ankylosis animal model is obtained. Modeling is carried out in the mode that the condyle process and the temporal bone glenoid fossa part fibrous cartilage and the articular disc are removed in a surgical operation mode, and the animal model established in a composite trauma mode fits the complex pathological environment of human diseases.

Systems, methods, and kits are described for repairing a fibrocartilage defect in a subject. The fibrocartilage defect is contacted with a first composition containing an oxidized and methacrylated glycosaminoglycan to form an imine bond between the glycosaminoglycan and the fibrocartilage defect, thereby coating the fibrocartilage defect with the glycosaminoglycan. The fibrocartilage defect coated with the glycosaminoglycan is then contacted with a mixture of a pre-polymer hydrogel composition containing a first crosslinking unit that, when polymerized, is capable of bonding to methacrylate and a hydrogel polymerization initiator composition, thereby forming a hydrogel that is covalently bonded to the glycosaminoglycan through methacrylate.

The application discloses a composition for repairing meniscus, which comprises a growth differentiation factor and emodin, improves inflammation and oxidative stress in a microenvironment, and promotes cell chondrogenic differentiation process. It is verified that the "core-shell" co-delivery nano carrier provided by the application uses mesoporous silica nanoparticles to wrap two bioactive compounds, emodin and a growth differentiation factor, and the latter is used for promoting fibrocartilage differentiation. The dual-drug nano delivery system provides sustained release of emodin and GDF, synergistically regulates a harsh inflammatory and oxidative environment, and promotes fibrocartilage regeneration in vitro and in vivo. Benefited from this, the application also combines a three-dimensionally printed meniscus-shaped PCL frame with the composition by using a cartilage-specific matrix hydrogel, constructs a reinforced stability meniscus replacement model, has a synergistic regulation on a cartilage immune microenvironment, and realizes successful repair of a whole meniscus replacement operation of an animal (rabbit).

The present invention relates to a bioink composition derived from fibrocartilage, a bone graft composition containing the same, and a method for producing the same. More specifically, it was confirmed that the inner, middle, and outer sides of the meniscus cartilage tissue partitioned by anatomical position each have different physicochemical properties, and a bioink composition for 3D printing having different physicochemical properties using the extracellular matrix derived from each partitioned tissue and a bone graft are provided, and these can be utilized by adjusting the physicochemical properties according to the purpose.

The present invention relates to an extracellular matrix powder derived from the meniscus and a graft for fibrocartilage tissue reconstruction and, more specifically, to an extracellular matrix powder derived from the meniscus and a graft for fibrocartilage tissue reconstruction, wherein the extracellular matrix powder can enhance fibrocartilage differentiation ability and fibrocartilage regeneration effects by controlling a content of powder derived from a specific region of the meniscus.

The present invention relates to a fibrocartilage-derived bioink composition, a bone graft composition containing same, and a manufacturing method therefor. More specifically, it is identified in the present disclosure that the inner, mid, and outer compartments of the meniscal cartilage tissue based on anatomical location differ from each other in terms of physicochemical property, whereby use is made of the extracellular matrix derived from each compartmentalized tissue to provide 3D-printing bioink compositions and bone grafts, each having distinct physicochemical properties, which can be utilized with controlled physicochemical properties according to desired applications.

The invention provides a preparation method of a hip joint polyurethane artificial glenoid lip, which comprises the following steps: carrying out 3D printing on polyurethane to prepare an annular polyurethane stent, and then modifying the surface of the annular polyurethane stent with polydopamine. According to the preparation method of the hip joint polyurethane artificial glenoid lip, the biocompatibility and cell adhesion of the hip joint polyurethane artificial glenoid lip are enhanced through a polydopamine (PDA) surface modification technology, and the hip joint polyurethane artificial glenoid lip is used for repairing acetabular glenoid lip injury and promoting fibrous cartilage regeneration.

The invention belongs to the field of tissue regeneration materials, and relates to a microenvironment-imitating composite scaffold and application thereof in anisotropic regeneration of meniscus cartilage. According to the scaffold, a meniscus-derived acellular extracellular matrix and a 3D printing polycaprolactone scaffold are combined, and the composite scaffold with excellent biological activity and mechanical stability is prepared; meanwhile, by utilizing a space occupying sacrifice strategy based on the temperature-sensitive poloxamer hydrogel, precise spatial arrangement of fibrochondrocytes and fibroblasts is realized, and the hexagonal meniscus tissue engineering scaffold with a heterogeneity spatial continuous structure and without an obvious interface is successfully constructed. Experiments prove that the stent effectively reproduces regional heterogeneity of natural meniscus, realizes gradient distribution of I / II type collagen and sulfated glycosaminoglycan, achieves bionic reconstruction of structure and function, and provides a promising conversion path for repair and reconstruction of functional meniscus.

The invention belongs to the technical field of high polymer materials, and particularly relates to a negative Poisson's ratio auxetic toughening hydrogel material as well as a preparation method and application thereof. The hydrogel material comprises a fiber skeleton and hydrogel bonded on the fiber skeleton, and the fiber skeleton has a cosine wave-shaped negative Poisson's ratio structure, specifically a periodic structure formed by cosine curves arranged in a staggered manner in the horizontal direction and the vertical direction. By regulating and controlling preparation parameters of the cosine wave-shaped fiber, the specific surface area of the fiber is large, the auxetic effect is obvious, the fiber and the hydrogel form abundant covalent grafting, and the cosine wave-shaped negative Poisson's ratio structure fiber is used for driving hydrogel molecular chains to be in oriented arrangement when being pulled to form an ordered structure, so that the strength and the toughness of the hydrogel are effectively improved; the prepared negative Poisson's ratio auxetic and toughened hydrogel material can be used as a surgical material, such as a material for replacing or repairing fibrous cartilage and / or fibrous connective tissue.

The invention discloses a cationic nano-carrier for penetrating fibrous cartilage as well as a preparation method and application of the cationic nano-carrier, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: mixing an ethylenediamine solution and a Boc-Lys (Fmoc)-OH solution, adding HATU and HOAt for activation, adding DIPEA for continuous activation, stirring for reaction, adding ice ether, and standing to obtain a precursor; mixing the precursor with a piperidine solution, adding ice diethyl ether and normal hexane, and standing to obtain DGL; dGL reacts in a mixed solution of TFA, DCM and TIS to prepare DGL after Boc removal, and DGL after Boc removal and phenylboronic acid react in a solvent to prepare the compound. The cationic nano-carrier prepared by the method can be reversibly combined with glycosaminoglycan in cartilage, can penetrate through the whole layer of temporal-mandibular joint condylar cartilage and simultaneously carries siRNA targeting deep cartilage cells, so that lysosome is endowed with escape ability, cartilage cell uptake is promoted, and siRNA transfection efficiency is improved.

The invention relates to the technical field of cartilage repair, and particularly discloses a cartilage repair stent and a preparation method thereof. The scaffold comprises a bone-facing surface part and a back bone surface part, wherein the bone-facing surface is a porous three-dimensional network structure formed by decellularizing bovine pericardium, porcine pericardium or horse pericardium, and I-type and III-type collagen is reserved; the back bone surface is a soft membrane layer made of cow leather, pigskin or horse skin and provides mechanical support. The preparation method comprises the following steps: selectively removing type II collagen through enzymolysis, enhancing the stability of the collagen through chemical treatment, and combining the two-phase structure through a freeze-drying process. After the stent is implanted, the regeneration of the autologous II-type collagen can be promoted in combination with a micro-fracture surgery, the mechanical property close to that of natural cartilage is formed, the problems of insufficient strength of fibrous cartilage, donor limitation, secondary injury and the like in traditional repair are effectively solved, and the cartilage repair effect and the life quality of a patient are remarkably improved.

The invention discloses application of amygdalin in inhibition of cartilage cell death and fibrosis and a composition. The invention discloses and confirms that cartilage cell ferroptosis is a key pathological mechanism for driving cartilage fibrosis and scar formation. The invention proves that the natural plant extract amygdalin can effectively inhibit cartilage cell ferroptosis induced by IL-17A and other factors, and the mechanism at least partially relates to regulation of IL-17A / GPX4 signal axis, especially by up-regulating expression of key anti-ferroptosis protein GPX4. The inhibition effect on ferroptosis causes the expression of a fibrocartilage marker, and meanwhile, the expression of a hyaline cartilage anabolism marker is improved, which indicates that AMD can be effectively used for preparing the anti-cartilage fibrosis medicine. The present invention discloses a pharmaceutical composition comprising AMD and Mg < 2 + >, AMD provides a synergistic or protective effect for the cartilage protection effect of Mg < 2 + > by inhibiting ferroptosis and exerting an antioxidant effect. The invention provides a new conservative treatment strategy which targets ferroptosis and has a good application prospect for the treatment of cartilage fibrosis.