6 results about "Fibrocartilage" patented technology

The invention relates to the technical field of biological medicine, in particular to a traditional Chinese medicine composition for promoting regeneration of hyaline cartilage and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-30 parts of prepared rehmannia root, 10-30 parts of astragalus membranaceus, 10-20 parts of Chinese yam, 10-20 parts of cistanche deserticola, 5-15 parts of angelica sinensis, 5-15 parts of corydalis tuber, 5-15 parts of spina date seed and 3-10 parts of safflower carthamus. The traditional Chinese medicine composition has the traditional effects of tonifying kidney and replenishing essence, tonifying qi and activating blood, and activating meridians to stop pain, and is proved to be capable of effectively relieving oxidative stress of a local microenvironment of joint injury and remodeling metabolic homeostasis of mesenchymal stem cells by up-regulating expression of HIF-1alpha and PLK1, so that regeneration of high-quality transparent cartilage is specifically induced, formation of fibrocartilage is inhibited, and the joint injury effect is improved. The composition is used for preventing or treating articular cartilage defects and osteoarthritis.

The invention discloses a bionic intervertebral disc integrated organoid based on 3D printing and a construction method and application thereof, and relates to the field of biomedical engineering. The construction method comprises the following steps that a polymer material is adopted to prepare a crossed net-shaped bionic stent through 3D printing, the crossed net-shaped bionic stent is curled to form a cylindrical fiber ring structure, and the fiber ring bionic stent is obtained; performing mesoderm induction on the pluripotent stem cells, and respectively performing cartilage induction and fibrous cartilage induction to obtain nucleus pulposus-like cartilage organs and fibrous ring-like fibrous cartilage organs; the cartilage-like organ is placed in the center of the fibrous ring bionic scaffold, the fibrous cartilage-like organ is filled in the grid structure of the fibrous ring bionic scaffold, and after matrigel wrapping and incubation curing, the bionic intervertebral disc integrated organ based on 3D printing is obtained through induction culture. The bionic intervertebral disc integrated organ based on 3D printing provided by the invention can effectively reproduce structural characteristics and functional characteristics of a natural intervertebral disc.

Systems, methods, and kits are described for repairing a fibrocartilage defect in a subject. The fibrocartilage defect is contacted with a first composition containing an oxidized and methacrylated glycosaminoglycan to form an imine bond between the glycosaminoglycan and the fibrocartilage defect, thereby coating the fibrocartilage defect with the glycosaminoglycan. The fibrocartilage defect coated with the glycosaminoglycan is then contacted with a mixture of a pre-polymer hydrogel composition containing a first crosslinking unit that, when polymerized, is capable of bonding to methacrylate and a hydrogel polymerization initiator composition, thereby forming a hydrogel that is covalently bonded to the glycosaminoglycan through methacrylate.

The present invention relates to an extracellular matrix powder derived from the meniscus and a graft for fibrocartilage tissue reconstruction and, more specifically, to an extracellular matrix powder derived from the meniscus and a graft for fibrocartilage tissue reconstruction, wherein the extracellular matrix powder can enhance fibrocartilage differentiation ability and fibrocartilage regeneration effects by controlling a content of powder derived from a specific region of the meniscus.

The present invention relates to a fibrocartilage-derived bioink composition, a bone graft composition containing same, and a manufacturing method therefor. More specifically, it is identified in the present disclosure that the inner, mid, and outer compartments of the meniscal cartilage tissue based on anatomical location differ from each other in terms of physicochemical property, whereby use is made of the extracellular matrix derived from each compartmentalized tissue to provide 3D-printing bioink compositions and bone grafts, each having distinct physicochemical properties, which can be utilized with controlled physicochemical properties according to desired applications.

The invention belongs to the field of tissue regeneration materials, and relates to a microenvironment-imitating composite scaffold and application thereof in anisotropic regeneration of meniscus cartilage. According to the scaffold, a meniscus-derived acellular extracellular matrix and a 3D printing polycaprolactone scaffold are combined, and the composite scaffold with excellent biological activity and mechanical stability is prepared; meanwhile, by utilizing a space occupying sacrifice strategy based on the temperature-sensitive poloxamer hydrogel, precise spatial arrangement of fibrochondrocytes and fibroblasts is realized, and the hexagonal meniscus tissue engineering scaffold with a heterogeneity spatial continuous structure and without an obvious interface is successfully constructed. Experiments prove that the stent effectively reproduces regional heterogeneity of natural meniscus, realizes gradient distribution of I / II type collagen and sulfated glycosaminoglycan, achieves bionic reconstruction of structure and function, and provides a promising conversion path for repair and reconstruction of functional meniscus.