52results about How to "No risk of infection" patented technology

The objective of the present invention is to provide a collagen material that possesses physical properties to an extent that allows suturing while still maintaining the biochemical properties inherently possessed by collagen, and retains its shape for a certain amount of time even after application to the body; its production process; and, a medical material on which it is based, examples of which include a artificial tube for nerve, artificial tube for spinal cord, artificial esophagus, artificial trachea, artificial blood vessel, artificial valve or alternative medical membranes such as artificial endocranium, artificial ligaments, artificial tendons, surgical sutures, surgical prostheses, surgical reinforcement, wound protecting materials, artificial skin and artificial cornea, characterized by filling or having inside a substance having biocompatibility that can be degraded and absorbed in the body into a matrix of a non-woven fabric-like multi-element structure of collagen fibers having ultra-fine fibers of collagen as its basic unit.

A blood vessel occlusion system and method includes a handheld probe including a power source, the probe being adapted to transmit energy from the power source through a body surface and into a target blood vessel, for example a varicose vein. Further included is a manual or automatic switch mechanism adapted to enable and disable the power source in response to a predetermined level of compression applied, by means of the probe against the target blood vessel. When the blood vessel is compressed sufficiently to collapse and substantially slow or substantially prevent a flow of blood therethrough, the power source is activated and the vessel is cauterized, leaving the blood vessel in a condition that is resistant to recanalization.

The invention discloses recombinant Bacillus subtilis for producing chondroitinase and application thereof and belongs to the technical field of bioengineering. Chondroitinase of common Proteus origin is heterologously expressed in Bacillus subtilis, signal peptide amyX is selected, 3 strong constitutive promoters and strong RBS (ribosome binding site) of pP43NMK vector itself are utilized, histidine tags are added to facilitate subsequent purification, and expressive enzyme activity of chondroitinase in Bacillus subtilis is improved. Certain basis is laid for efficiently producing chondroitinase from food-grade microbes, and the recombinant Bacillus subtilis is suitable for industrial application.

The invention relates to a separable hydrogel microneedle loaded with recombinant human fibronectin and a preparation method and application thereof, the separable hydrogel microneedle loaded with recombinant human fibronectin comprises a microneedle substrate and a microneedle tip separably attached to the microneedle substrate, the microneedle tip is formed by crosslinking and curing a hydrogel precursor solution through UV initiation, and the hydrogel precursor solution comprises a hydrogel material, a carrier delivery protein, a photoinitiator and an aqueous medium; the delivery protein is recombinant human fibronectin obtained through genetic engineering. A hydrogel material, carrier delivery protein, a photoinitiator and an aqueous medium are subjected to UV-induced crosslinking curing to obtain the separable microneedle tip, the microneedle tip can be retained in a body after application, and a carrier drug embedded in the microneedle tip is released to surrounding tissues through diffusion. And retention and slow release of functional drugs are realized.

The invention provides a method for manufacturing a material for repairing the human tissue by adopting autologous fingernails. The method mainly comprises the following steps: washing the autologous fingernails to be clean, carrying out disinfecting and sterilizing on the washed autologous fingernails, dehydrating the fingernails until the water content is less than 50%, immersing the dehydrated fingernails into liquid nitrogen, grinding the fingernails by adopting a grinder until the particle diameter is less than 5000 [mu] m, and screening the particles by adopting a sieve so that matrix and powder are separated, wherein the powder can be used as the material for repairing the human tissue. The powder biological material manufactured by adopting the method provided by the invention is rich in collagen, collagen is the important substance for maintaining the human tissue structure, the powder biological material is manufactured by adopting the autologous fingernails, so as to be easily absorbed by the human body, the host immune response can not be caused, further, the danger of rejection or infection is avoided, in addition, the acquisition source is simple, so that the material can be used as the most ideal material for human tissue repair or tissue transplantation, or other purposes.

The invention discloses an implantable left ventricular assisting system which comprises an in-vivo module and an in-vitro module. No transmission lines exist between the in-vivo module and the in-vitro module. The in-vitro module comprises a strong current power supply unit, a power supply end power converting unit, a resonant transformation unit, a primary side coil of a loosely coupled transformer, a power supply end weak current power supply unit, a power supply end controller, a switch tube drive unit, an optical coupler isolating unit, a current sampling unit, a power supply end signal conditioning unit, a protecting unit, a battery state display module and a demodulator for receiving battery state information. The in-vivo module comprises an auxiliary side coil of the loosely coupled transformer, a receiving end resonant transformation unit, a receiving end power conversion unit, a receiving end controller, a battery charging management module, a battery, a modulator and a blood pump. The implantable left ventricular assisting system is simple and compact in structure, small in size and lightweight, and subcutaneous wire holes of a patient can be avoided.

The invention discloses a heparin precursor synthase and application thereof and belongs to the technical field of bioengineering. Heparin precursor synthases KfiC and KfiA from escherichia coli K5 are modified in three ways in bacillus subtilis, i.e., the two synthases are directly fused; the two synthases are connected through a flexible linker; and key areas in the KfiC and KfiA replace a key area of a PmHS synthase from pasteurella multocida to obtain three novel heparin precursor synthases, and the results that the yield of heparin precursors is improved and the molecular weight of the heparin precursors is reduced are realized. The invention lays a foundation for efficiently producing and preparing the heparin precursors from food grade microorganisms, and the heparin precursor synthase is suitable for industrial production and application.

The invention discloses a hospital wastewater comprehensive treatment device with zero sludge discharge and a treatment method thereof. The device is composed of a chlorine dioxide disinfection tank,a three-grid septic tank, an adjusting tank, a closed ultraviolet sterilizer, a multistage bio-chemical tank and a chlorine dioxide disinfection tank which are sequentially connected in series, and further comprises a waste gas treatment module; a cutting pump is arranged in the adjusting tank; the waste gas treatment module is composed of plasma equipment and activated carbon adsorption equipment; wherein the multi-stage biochemical tank is formed by connecting a multi-stage unit A, a multi-stage unit O and a precipitation unit in series, part of sludge at a sludge outlet of a precipitation unit flows back to the front section of the multi-stage unit A through a sludge return pump and a sludge return pipe, and the rest sludge is discharged to the water inlet section of the three-grid septic tank as residual sludge; by adopting the treatment method disclosed by the invention, the effluent quality is superior to the standard of Discharge Standard of Water Pollutants for Medical Organization (GB18466-2016), the treatment method has the advantages that residual sludge, grid slag and the like are not generated, and the risk of bacterial infection of aerosol, sludge, grid slag, waste gas and the like is avoided.

The invention discloses a chondroitinase ABC producing recombinant yeast strain and application thereof and belongs to the technical field of bioengineering. According to the chondroitinase ABC producing recombinant yeast strain, chondroitinase ABC from Proteus vulgaris ATCC33420 is subjected to heterogeneous expression, and with pPIC9K carriers and through induction of methanol, secretory expression of the chondroitinase ABC in pichia pastoris GS115 can be achieve; by means of a designed chondroitinase ABC-enzymatic membrane reactor, efficient and continuous production of small-molecular chondroitin sulfate can be achieved, the chondroitinase ABC can be recycled and reutilized, and the production cost can be greatly reduced. By taking food-grade yeast as host strains, the chondroitinase ABC producing recombinant yeast strain can be safe and reliable, provide effective references for industrialized and green production of small-molecular chondroitin sulfate A, B and C, and meanwhile, save energy, reduce emission and achieve significant economic and social benefits.

This invention relates to a standard fluid for a flow cytometer is characterized by showing, after staining, almost the same fluorescence intensity and scattered light intensity as those of the cells to be assayed. The present invention aims at providing a standard fluid for a flow cytometer which shows almost the same behaviors as those of the cells to be assayed and can be easily handled without any risk of infection.

The present invention provides a non-invasive lip beautifying method. The non-invasive lip beautifying method comprises the following steps; step 1, uniformly smearing a penetration enhancer on lips to be beautified, and changing cell arrangement of stratum corneum of lips; step 2, smearing liposome containing pure natural plant toner emulsion on part of lip area to be beautified; and step 3, applying electric pulses or ultrasonic waves on the liposome containing pure natural plant toner emulsion smeared on the lips to be beautified, enabling the pure natural plant toner emulsion to penetrateinto the lip skin, and beautifying the different partitions of the lips with different force. By differentiated stimulation on the lips in partitions, the pure natural plant toner emulsion is fused ina non-invasive mode, and the three-dimensional lip beautifying is achieved. The whole lip beautifying process is non-invasive, free of pain and swelling, and avoids risk of scars.

Provided is a sterile article placing device. At present, when an operation is carried out, the number of surgical instruments and objects is large, the surgical instruments and objects cannot be placed on a plane, nurses need to count for four times, and counting errors are easily caused. The device comprises: a disinfection and sterilization basket (1), a tray (2), an antenna frame (3), a Z-shaped support (4) and a clamping part (6), wherein a bottom of the Z-shaped support is in threaded connection with the clamping part, a top of the Z-shaped support is in threaded connection with a center of the antenna frame, the tray and the antenna frame are fixed in an inserted mode, the disinfection and sterilization basket is arranged on the tray, and an insertion column (10) at a top of each antenna of the antenna frame is inserted into a grid of the disinfection and sterilization basket. The device is used for placing sterile articles.

The invention discloses a recombinant engineering bacterium for producing 2 '-fucosyllactose as well as a construction method and application of the recombinant engineering bacterium, and belongs to the technical field of genetic engineering. The invention discloses a recombinant engineering bacterium for producing 2 '-fucosyllactose, the strain name of the recombinant engineering bacterium is KMdeLac24-FL15, the recombinant engineering bacterium is preserved in the China General Microbiological Culture Collection Center on March 21, 2022, the preservation number is CGMCC NO.24549, and the recombinant engineering bacterium is classified and named as Kluyveromyces marxianus. The invention further discloses a preparation method of the recombinant engineering bacterium for producing 2'-fucosyllactose. The strain can efficiently express the 2 '-fucosyllactose and is applied to preparation of the 2'-fucosyllactose. The invention also discloses a recombinant engineering bacterium for producing the 2 '-fucosyllactose and a construction method of the recombinant engineering bacterium, and also discloses application of the recombinant engineering bacterium in preparation of the 2'-fucosyllactose.

The invention relates to the field of aquaculture technology and fishery independent innovation, and discloses a cultivation method for twice gonad maturation of micropterus salmoides within one year, which comprises the steps of parent selection and temporary rearing, culture water temperature reduction in summer, culture water temperature increase in autumn and winter, illumination time adjustment, parent nutrition supplement adjustment, gonad maturation index calculation and the like. The sexual glands of micropterus salmoides are induced to develop out-of-season maturity, and the requirement that out-of-season breeding has serious lack of parents is met. Through a water temperature adjusting technology, a natural illumination simulation technology and a nutrition enrichment technology, the water temperature, illumination and nutrition requirements of seasonal changes in the nature for gonad development of fishes are simulated, the gonads of micropterus salmoides are induced to mature for the second time within one year, and the purpose of providing sufficient parents required by out-of-season breeding is achieved. And support is provided for expanding the micropterus salmoides culture scale and realizing adult fish culture in the current year.