Quality control material for detecting respiratory tract pathogen nucleic acid and preparation method thereof

Abstract

The invention provides a quality control material for detecting respiratory pathogen nucleic acid. The quality control material comprises any one or more of the following respiratory pathogens: coronavirus, influenza virus, adenovirus, mycoplasma pneumoniae, streptococcus pneumoniae, rhinovirus or / and legionella pneumophila. A full-length genome sequence of the coronavirus is divided into six target fragments, and the length of each fragment ranges from 4000 bp to 5500 bp. The quality control material disclosed by the invention covers all detection target sequences (or target spots) of the coronavirus, the coverage is wide, the detection target spots are comprehensive, and the phenomenon of missing detection is avoided. The quality control material provided by the invention contains main respiratory tract infection pathogens, has a wide coverage range, and has more accurate detection targets by taking a real virus sample and a slow virus sample as raw materials. According to the coronavirus quality control material prepared by the invention, a target gene sequence is integrated into a host genome through a lentiviral vector, an autonomously replicated gene is knocked out in the preparation process, and the coronavirus quality control material has a self-inactivation capability, so that the recombinant lentivirus cannot be replicated in a target cell and infects other cells.

Description

Technical field

[0001] The present invention belongs to the technical field of medical test, and more particularly to a mass control for detecting a respiratory pathogen nucleic acid and a preparation method thereof.Background technique

[0002] Respiratory infection is the most common infectious disease, caused by respiratory infectious diseases, mainly pathogens causes respiratory infection by respiratory system such as nasal cavity, trachea. Respiratory viruses refers to a large class of viruses caused by acute respiratory tissue infection or other tissue organ lesions in respiratory mucosa epithelial cell proliferation. According to statistics, 90% of the original acute upper respiratory tract infection is caused by respiratory viruses, and the virus triggers the lower respiratory tract infection with 62%. Studies have shown that some respiratory pathogens, such as Middle Eastern respiratory syndrome viruses, avian influenza viruses, etc., due to the spread of the infection of these v...

Images