74 results about "Mycoplasmal pneumonia" patented technology

The invention discloses a special diluent for swine mycoplasmal pneumonia vaccines. The special diluent mainly contains a recombinant P97R1 protein immunostimulating complex (ISCOM-P97R1); and in addition, the special diluent also possibly contains other water-based adjuvants comprising levomisole, astragalus polysaccharide or/and carbomer. 1 ml of diluent solution contains 0.1-1 mg of QuilA, 0.02-0.2 mg of cholesterol, 0.02-0.2 mg of phosphatidylcholine and 0.4-4 mg of P97R1 antigen; and in addition, 1 ml of diluent solution also possibly contains 2-20 mg of levomisole, 20-100 mg of astragalus polysaccharide or/and 3-10 mg of carbomer and a phosphate buffer solution for the rest. The diluent is specially used for dissolving or diluting the swine mycoplasmal pneumonia vaccines comprising a live vaccine and an inactivated vaccine, has the double effects of complementing vaccine antigen protection spectrums and improving the vaccine cell and humoral immunostimulation capability, can remarkably improve the toxic attack protection efficacy of the vaccines, and also has the advantages of being simple and convenient to operate, good in syringeability and free from remarkable toxic side reaction due to the adoption of a hydrosolvent system at the same time.

This invention provides an immunogenic composition including a soluble portion of a Mycoplasma hyopneumoniae (M.hyo) whole cell preparation, wherein the soluble portion of the M.hyo preparation is substantially free of both (i) IgG and (ii) immunocomplexes comprised of antigen bound to immunoglobulin.

The invention discloses feed for preventing and treating mycoplasmal pneumonia of swine and a traditional Chinese medicine composition as well as a preparation method. The feed comprises a feed carrier, a feed adhesive and the traditional Chinese medicine composition, wherein the traditional Chinese medicine composition comprises meadowrue root and rhizome, common andrographis herb, cordate houttuynia, wild buckwheat rhizome, calyx seu fructus physalis, agilawood, perillaseed, fingered citron, bulbus fritillariae cirrhosae, snakegourd fruit, rhizoma anemarrhenae, bamboo juice, tabasheer, platycodon grandiflorum, concha meretricis seu cyclinae, bryozoatum, aster, birthwort, folium eriobotryae, herba ardisiae japonicae, folium rhododendri daurici, radix astragali, liquorice, herb of little platanthera, pueraria montana merr and all-grass of longsepaled crotalaria. The feed has the beneficial effects that the feed overcomes various defects of traditional Chinese medicines and western medicines in the prior art, has the effects of clearing heat and eliminating phlegm, relieving cough and asthma, strengthening body resistance and eliminating evil on treatment of mycoplasmal pneumonia of swine and has a remarkable clinical effect, is high in curative ratio and has no toxic or side effects and no drug residue, no drug resistance is generated by using the feed, and the mycoplasmal pneumonia of swine is difficult to relapse by using the feed; in addition, the feed is capable of improving the immunity of organisms of ill pigs and is low in cost, and meat of pigs fed by using the feed has a certain health effect on bodies of customers eating the meat.

The invention provides a fusion protein, a fusion protein-containing vaccine composition for mycoplasmal pneumonia of swine (MPS) and an application of the vaccine composition. The fusion protein comprises pseudomonas exotoxin A structural domains I and II, an antigenic protein for MPS and a carboxyl terminal part, wherein the antigenic protein for MPS comprises any one of an NrdF protein, a P102 protein, a P97R1 protein, a P97R2 protein, a P97R1-NrdF protein and a P97R2-NrdF protein. The invention also discloses the vaccine composition containing immune dosage of fusion protein and the application of the vaccine composition to preparation of drugs for preventing and/or treating MPS. The vaccine composition has the advantages that recombinant expression can be carried out on the components of the vaccine composition in quantity by means of genetic engineering, thus not only consuming short time but also facilitating large-scale production; the vaccine composition can generate humoral immunity and cellular immunity and can gain better immune effects.

The invention discloses a collaborative quadruple real-time fluorescence PCR detection method for simultaneously detecting target nucleic acids of nine pathogens by using three PCR reaction tubes. Themethod is used for detecting influenza A virus (FluA), influenza B virus (FluB), parainfluenza virus (PIV), respiratory syncytial virus (RSV), mycoplasma pneumoniae (MP), chlamydia pneumoniae (Cpn),adenovirus (ADV), human rhinovirus (HRV) and human metapneumovirus (hMPV) in samples. The method is rapid to operate, can be completed by one-step cooperation and has high sensitivity. In addition, the invention also relates to a reagent involved in the PCR method, a detection kit used for the method, preparation and application of the detection kit and the like.

The invention discloses a nanoemulsion composition for treating mycoplasma pneumonia of livestock, which consists of raw materials including, by mass percent, from 0.1 to 8.0% of amyris oil, from 0.1 to 5.0% of vitex oil, from 0.1 to 3.0% of perilla oil, from 0.1 to 2.0% of bupleurum oil, from 0.01 to 0.1% of allicin, from 0.1 to 4.0% of tiamulin fumarate, from 19.0% to 31.0% of surfactant and the balance distilled water. The sum of the mass ratios of the ingredients is 100%. The nanoemulsion composition has effects of dilating the trachea of the livestock, suppressing cough reflex, increasing immunity of the organism, resisting inflammation and killing mycoplasma pneumoniae or other mixed infected causative agent, can be used for preventing and curing respiratory diseases including chronic respiratory diseases of the livestock, mycoplasma pneumoniae of swine, porcine contagious pleuropneumonia and the like, is fine in stability, is high in intestinal absorption utilization rate after being orally taken, can avoid liver first pass effect, and is remarkable in curative effect.

The invention provides a feed for treating mycoplasmal pneumonia of swine, wherein the feed comprises traditional Chinese medicine as a feed additive and a feed carrier; the traditional Chinese medicine comprises cassia twig, ginger, anemarrhena, rhizoma phragmitis, cortex dictamni, forsythia, blackberry lily, toad, salvia miltiorrhiza, radix cyathulae, pinellia ternata, inula japonica thunb, ginkgo, codonopsis, licorice, loquat leaf and wild buckwheat. Relative to 10 parts by weight of feed carrier, the additive amount of the traditional Chinese medicine can be 4-8 parts by weight. The feed containing the traditional Chinese medicine composition for treating mycoplasmal pneumonia of swine has the advantages of high efficiency, low toxic and side effects, no residue, no generation of drug resistance, no environment pollution, short treatment course and no repetition of illness state, and organism resistance can be increased, and the like.

The invention provides a lincomycin hydrochloride-spectinomycin sulfate injection and a preparation method thereof. Water for injection is taken as a carrier; and 100ml of injection contains 3 to 7g of lincomycin hydrochloride and 8 to 12g of spectinomycin sulfate. The invention has the advantages that the injection is safe and reliable, and has low toxic or side effect and high bioavailability; lincomycin hydrochloride-spectinomycin sulfate is prepared into a compound hydro-acupuncture injection, the injection has a synergistic effect and a relatively wide antibacterial spectrum, can remarkably strengthen the antibacterial activity on mycoplasmas, can act quickly, and has low toxicity, and the therapeutic dose of the injection does not have adverse affect on chickens and pigs; and the injection has a good effect of treating respiratory diseases of livestock, particularly mycoplasmal pneumonia, digestive tract diseases of the livestock, and chicken chronic respiratory diseases which are caused by mycoplasma gallisepticums, is favorable for reducing the medicine tolerance of medicines, and prolongs the service life of the medicines.

The invention provides a preparation method, a formula and a use method of a bovine mycoplasma pneumonia inactivated vaccine. The preparation method of the bovine mycoplasma pneumonia inactivated vaccine comprises the following steps: 1, selecting diseased cattle which naturally infect mycoplasma pneumonia and is dying, taking diseased lungs, lymph glands and spleens to homogenize and removing precipitates and grease through filtering and centrifugation to obtain an antigen solution; 2, adding formaldehyde into the antigen solution to inactivate for 72 hours, adding 1,000 units of penicillin and 1,000 units of streptomycin into the antigen solution per millimeter and uniformly mixing to obtain an inactivated antigen solution; 3, mixing the inactivated antigen solution with an aluminum hydroxide adjuvant according to the proportion of 4:1 to obtain the inactivated vaccine. The vaccine prepared by the preparation method is safe in application, strong in pertinence and indeed in immune protection effect on mycoplasma causing bovine pleuropneumonia by infection, the lung lesion score of the infected cattle can be obviously reduced, the material weight ratio is improved, and the good effects of prevention and disease control can be achieved.

The invention provides a primer group for multiple RPA detection of respiratory tract pathogens, a detection reagent and a kit. The high-specificity primer group for multiple RPA detection is successfully obtained through RPA primer design improvement of at least 5 respiratory tract pathogens of human cytomegalovirus, adenovirus, parainfluenza virus 1, parainfluenza virus 2, parainfluenza virus 3,respiratory syncytial virus, staphylococcus aureus, streptococcus pneumoniae, mycoplasma pneumoniae, chlamydia pneumoniae, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, stenotrophomonas maltophilia and legionella pneumophila, the detection reagent is prepared with the adoption of the primer group, and the kit is prepared, so that RPA detection of up to 15 respiratory tract pathogens can be realized according to speculation, time required for the whole detection procedure can be shortened, and steps are simplified.

The invention relates to the field of breeding of live pigs, and specifically discloses epidemic disease prevention feed for live pigs and a preparation method thereof. The feed disclosed by the invention can be added to the feed for live pigs, the live pigs can quickly intake the components on the feed, the resistibility of the live pigs to diseases can be effectively enhanced before the live pigs suffer from the diseases, and the risk that the live pigs suffer from colibacillosis, swine plague, mycoplasmal pneumonia of swine, porcine reproductive and respiratory syndrome and other diseases can be greatly reduced The scheme can improve the resistibility of the live pigs to epidemic diseases.

The invention discloses a traditional Chinese medicine composition used for preventing and treating mycoplasmal pneumonia of swine. The traditional Chinese medicine composition comprises the following components in parts by weight as main active components: 20-30 parts of herba houttuyniae, 15-25 parts of membranous milkvetch roots, 15-25 parts of radix stemonae, 15-20 parts of platycodon grandiflorum, 15-20 parts of garlic, 10-20 parts of rhizoma anemarrhenae, 10-15 parts of ephedra, 10-15 parts of roots of redroot gromwell, and 5-10 parts of radix glycyrrhizae. The invention also discloses a preparation method and application of the traditional Chinese medicine composition. Tests show that the traditional Chinese medicine composition can inhibit in-vitro growth of mycoplasma hyopneumoniae, can well control the mycoplasma hyopneumoniae for infection and pathogenicity of mice, and is clinically used for preventing and treating infection and pathogenicity of the mycoplasma hyopneumoniae, has a good pharmaceutical effect and no toxic or side effects, and is convenient to take.

The invention discloses an injection of compound micironomicin sulfate, its preparation method and application. the injection comprises (by weight part) micironomicin sulfate injection 1-20, chlorpheniramine maleate 0.1-1, and dexamethasone sodium phosphate 0.01-1. the preparation method comprises heating 50% water for injection to 40-50deg.C, dissolving each components, regulating pH to 3.5-6.5 with hydrochloric acid or sodium hydroxide, and adding water for injection to 1000. The invention has the advantages of rational formulation, reduced drugfastness, and shortened withholding period. It can enhance therapeutic effect, improve swinish immunity, increase swinish body weight; and can be used for treating mycoplasmal pneumonia of pig.

The invention discloses a veterinary compound florfenicol powder and a preparation method thereof, which aims at providing the veterinary compound florfenicol powder that has rapid onset of action for the treatment for porcine infectious pleuropneumonia and mycoplasmal pneumonia and can treat both manifestation and root causes of the diseases, alleviate the stress reactions when having the attacks of diseases of the pigs and improve the weight gain rate, and the preparation method that has simple process and easy realization. The powder is composed of the components with the following weight percentages: 5 to 20 percent florfenicol, 0.5 to 5 percent doxophylline and the rest of starch. The powder of the invention is a compound preparation which combines anti-bacterial drugs and the drugs for relieving cough and asthma, the florfenicol and the doxophylline are combined for usage, thus having rapid onset of action for the treatment for infectious pleuropneumonia and mycoplasmal pneumonia, effectivity and high efficiency; furthermore, the power can ease the stress reactions of the affected animals, significantly ease the clinical symptoms caused by the infectious pleuropneumonia and the mycoplasmal pneumonia, treat both manifestation and root causes of diseases, reduce the suffering of the affected pigs and increase the weight of the pigs.

The invention relates to tiamulin fumarate effervescent granules for treating pneumonia and dysentery of livestock and poultry, which comprise the following raw materials and auxiliary materials in part by weight: 80 to 120 parts of tiamulin fumarate, 100 to 140 parts of citric acid, 150 to 170 parts of sodium bicarbonate, 20 to 40 parts of polyethylene glycol 6000, 190 to 230 parts of dextrin and 360 to 400 parts of sucrose. A method for preparing the tiamulin fumarate effervescent granules comprises the following steps of: grinding the raw materials and the auxiliary materials, respectively mixing the ground raw materials and the ground auxiliary materials, drying the mixture, pelletizing the dried mixture and sufficiently mixing the products. The tiamulin fumarate effervescent granules prepared by the method are used for diseased livestock and poultry in an oral administration mode, have the advantages of good taste and stable quality and are an ideal preparation for treating mycoplasma pneumonia, actinomycotic pleuropneumonia and treponema dysentery of the livestock and poultry currently.

The invention relates to a selective separation medium for caprine mycoplasmal pneumonia subspecies and a preparation method of the selective separation medium, and belongs to the technical field of veterinary biology. The selective separation medium is prepared from components as follows: lactoalbumin hydrolysate, yeast extract powder, glucose, an MEM medium, a PPLO (pleuropneumonia-like organism) gravy powder, sodium pyruvate, a 1% phenol red solution, specific growth inhibition serum and deionized water. The preparation method comprises steps as follows: the lactoalbumin hydrolysate, the yeast extract powder, glucose, the MEM medium, the PPLO gravy powder, sodium pyruvate and the 1% phenol red solution are weighed in proportion and dissolved in the deionized water. Before use, healthy horse serum and the specific growth inhibition serum are added. The selective separation medium for the caprine mycoplasmal pneumonia subspecies is used for clinically separating and cultivating the caprine mycoplasmal pneumonia subspecies, increases the growth speed of mycoplasma in a separation process and increases the separation rate of the caprine mycoplasmal pneumonia subspecies, and the separation rate is 80% or higher.

The invention relates to a medicinal composition for treating mycoplasmal pneumonia and application thereof. The medicinal composition is prepared from the following Chinese herbs in part by weight: 26-40 parts of cordate houttuynia, 26-40 parts of blackberry lily, 18-30 parts of Chinese arborvitae twig, 15-25 parts of scutellaria baicalensis, 18-30 parts of duckweed, 18-30 parts of dried orange peel, 8-16 parts of ephedra and 8-16 parts of moutan bark. The medicinal composition is matched with erythrocin for treating the pediatric mycoplasmal pneumonia, and can effectively shorten the defervescence time, the cough disappearing time and the lung rale disappearing time of a patient and reduce pains of an ill child.

The invention discloses Chinese herbal medicine feed for pigs and a preparation method of the feed. The feed is prepared from raw materials as follows: corn starch, wheat bran, soybean meal, rapeseed meal, radix astragali, fructus lycii, radix angelicae sinensis, poria cocos, fructus psoraleae, rhizoma atractylodis, silymarin, flastem milkvetch seeds, rhizoma cimicifugae, pomegranate rind, rape pollen, piper laetispicum, xylo-oligosaccharide, arabinose and galactose. On the basis of the corn starch, the wheat bran, the soybean meal and the rapeseed meal, a Chinese herbal medicine formula and saccharides are selected, a specific Chinese herbal medicine extraction method is adopted, and the prepared feed can treat PRRS (porcine reproductive and respiratory syndrome), mycoplasmal pneumonia of pigs and other diseases and can increase the content of protein and other nutrients in pork and the content of iron in pig blood.

The present invention provides an acetylisovaleryltylosin tartrate formulation preparation method, which comprises: (1) adding a wall material to water, and dissolving to prepare a binder solution, wherein the wall material comprises polylactic acid and arabic gum according to a ratio of 1:1; (2) adding an acetylisovaleryltylosin tartrate core material to the binder solution, and uniformly mixing to prepare a mixture; and (3) carrying out spray drying of the mixture prepared in the step (2) to prepare the acetylisovaleryltylosin tartrate formulation. According to the present invention, the acetylisovaleryltylosin tartrate formulation prepared through the method is stable and lastly act, and the cure rate is extremely high when the composition comprising the acetylisovaleryltylosin tartrate formulation and doxycycline is used to treat swine mycoplasma pneumonia.

The invention discloses a vaccine strain for mycoplasmal pneumonia of swine. The vaccine strain for mycoplasmal pneumonia of the swine, the strain number of which is CJ, is named as mycoplasma hyopneumoniae by classification, and preserved in the China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms (CCCCM) with the preservation number of CGMCC No.9909 on November 6th of 2014. The mycoplasma hyopneumoniae CJ strain grows rapidly and is high in content of bacteria during the preparation of an improved friis liquid medium; moreover, the mycoplasma hyopneumoniae has good immunogenicity and can be prepared into a prophylactic veterinary biological product or applied to veterinary drugs.

The invention relates to a traditional Chinese medicine compound injection with the effects of clearing heat, removing toxicity, stopping cough and calming asthma, including three medicines of Chinese lobelia herb, Philippine violet herb and honeysuckle flower. The invention is mainly used for livestock respiratory diseases. The invention is applicable to livestock virus flu, mycoplasmal pneumonia, bronchitis, lobar pneumonia and other diseases which are caused by viral and bacterial infections and flu and are accompanied with dyspnoea, cough, asthma, runny nose and other symptoms. The formula of the invention has the effects of clearing heat, removing toxicity, stopping cough and calming asthma. The medicine is prepared into the injection by the combination of the special physiological structure of the livestock, and the invention can be absorbed rapidly after muscle injection, so as to achieve the purpose of rapid onset of action.