Preparation method, formula and use method of bovine mycoplasma pneumonia inactivated vaccine

An inactivated vaccine, Mycoplasma bovis technology, applied in the directions of drug combination, bacterial antigen components, respiratory system diseases, etc., can solve the problems of economic loss of cattle industry, lack of preventive vaccines, weak effect, etc., and achieve a certain immune protection effect, Strong pertinence and the effect of improving the ratio of feed to meat

Inactive Publication Date: 2015-08-26
福清市默克兽医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cattle of any age and breed are susceptible, causing huge economic losses to the cattle industry
Mycoplasma, also known as mycoplasma, has a cell structure between viruses and bacteria, and conventional antibacterial and antiviral drugs have little effect on it, and there is currently no vaccine to prevent the disease

Method used

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  • Preparation method, formula and use method of bovine mycoplasma pneumonia inactivated vaccine
  • Preparation method, formula and use method of bovine mycoplasma pneumonia inactivated vaccine
  • Preparation method, formula and use method of bovine mycoplasma pneumonia inactivated vaccine

Examples

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Comparison scheme
Effect test

Embodiment 1

[0024] Preparation method of bovine mycoplasma pneumonia tissue inactivated vaccine

[0025] 1. Select a cow that is diagnosed with mycoplasma bovis pneumonia and is dying, take out the diseased lung, lymph node and spleen, remove the connective tissue under aseptic conditions, and cut it into 10mm pieces 3 For the left and right small pieces, add 1000mL of 4°C pre-cooled physiological saline at a ratio of 1:5 (m / v), and homogenize in a tissue homogenizer to obtain a tissue homogenate;

[0026] 2. Freeze and thaw the tissue homogenate 3 times continuously;

[0027] 3. Filter the tissue homogenate that has been frozen and thawed three times through a 150-mesh filter to obtain about 1500 mL of filtrate;

[0028] 4. After the filtrate was left to stand overnight at 4°C, remove the grease on the upper part, and then centrifuge at 4000rpm for 1 hour at a temperature of 4°C to remove the precipitate, and obtain the antigen solution for preparing vaccines;

[0029] 5. Add 37% forma...

Embodiment 2

[0056] 1. Select cows that are diagnosed with mycoplasma bovis pneumonia and are dying, take out the diseased lungs, lymph nodes and spleens, and store them at -20°C for later use;

[0057] 2. Thaw the organ obtained in step 1, remove the connective tissue under aseptic conditions, cut it into small pieces of about 10mm3, add 1000mL of sterilized saline pre-cooled at 4°C at a ratio of 1:5 (m / v), and put it in the tissue Homogenize in a homogenizer to obtain tissue homogenate;

[0058] 3. Freeze and thaw the tissue homogenate 3 times continuously;

[0059] 4. Filter the tissue homogenate that has been frozen and thawed three times through a 150-mesh filter to obtain about 1500 mL of filtrate;

[0060] 5. After the filtrate was allowed to stand overnight at 4°C, remove the grease on the upper part, and then centrifuge at 4,000 rpm for 1 hour at a temperature of 4°C to remove the precipitate, and obtain the antigen solution for preparing vaccines;

[0061] 6. Add 37% formaldehy...

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Abstract

The invention provides a preparation method, a formula and a use method of a bovine mycoplasma pneumonia inactivated vaccine. The preparation method of the bovine mycoplasma pneumonia inactivated vaccine comprises the following steps: 1, selecting diseased cattle which naturally infect mycoplasma pneumonia and is dying, taking diseased lungs, lymph glands and spleens to homogenize and removing precipitates and grease through filtering and centrifugation to obtain an antigen solution; 2, adding formaldehyde into the antigen solution to inactivate for 72 hours, adding 1,000 units of penicillin and 1,000 units of streptomycin into the antigen solution per millimeter and uniformly mixing to obtain an inactivated antigen solution; 3, mixing the inactivated antigen solution with an aluminum hydroxide adjuvant according to the proportion of 4:1 to obtain the inactivated vaccine. The vaccine prepared by the preparation method is safe in application, strong in pertinence and indeed in immune protection effect on mycoplasma causing bovine pleuropneumonia by infection, the lung lesion score of the infected cattle can be obviously reduced, the material weight ratio is improved, and the good effects of prevention and disease control can be achieved.

Description

technical field [0001] The invention relates to a preparation method, a formula and a use method of a tissue inactivated vaccine, in particular to a preparation method, a formula and a use method of a tissue inactivated vaccine for preventing bovine mycoplasma pneumonia. Background technique [0002] Mycoplasma bovis pneumonia, also known as bovine infectious pleuropneumonia or bovine pneumonia, is a highly contagious infectious disease of cattle caused by mycoplasma filamentous. The disease is characterized by exudative inflammation of pulmonary interstitial lymphatic vessels, connective tissue, and alveolar tissue, as well as serous fibrinous pleuropneumonia. In addition, it can also cause arthritis, mastitis, keratitis and reproductive system diseases. Cattle of any age and breed are susceptible, causing huge economic losses to the cattle industry. Mycoplasma, also known as Mycoplasma, has a cell structure between viruses and bacteria. Conventional antibacterial and ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A61P11/00A61P31/04
Inventor 张德明林燕花王锦祥李金凤车勇良张梦青
Owner 福清市默克兽医院
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