The invention belongs to the field of control of animal borne diseases, and relates to a mutant having reduced adhesive ability and with a deleted mycoplasma bovis gene. The protein gene Mbov_0503 isobtained by cloning from a mycoplasma bovis HB0801 genome. According to the partiality of escherichia coli for codons, the Mbov_0503 gene is modified, and mycoplasma bovis tryptophan codon UGA is mutated into a codon UGG for coding tryptophan in the escherichia coli to obtain a recombinant protein Mbov0503. The sequence of the protein gene which is cloned is shown as SEQID NO:13, and the sequenceof the coded protein is shown as SEQID NO:14. The mutant provided by the invention is an adhesion deficient strain screened from a mutant bank. The mutant has adhesive ability for host EBL cells, andcompared with a wild strain, for the mutant disclosed by the invention, the transmembrane transmission capacity for MDBK cells and the destructivity for tight connection between cells are notably reduced. The mutant can be applied to pathopoiesis and control of the mycoplasma bovis.