Mycoplasma bovis and application thereof

A technology of Mycoplasma bovis and inactivated vaccine, applied in veterinary vaccines, bacterial antigen components, antibody medical components, etc., can solve the problems of ineffective vaccine prevention, poor curative effect, economic loss of cattle industry, etc., and achieve good protection effect , Good immunogenicity and strong pathogenicity

Active Publication Date: 2017-07-07
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since 2008, the bovine respiratory infectious disease with fever, cough and runny nose as the main symptoms and necrotizing pneumonia as the main lesion has occurred and spread widely in many places in my country. It has also been identified as Mycoplasma bovis pneumonia caused by Mycoplasma bovis infection, Due to the poor curative effect of conventional drugs and no effective vaccine for the prevention of the disease, it has caused serious economic losses to the cattle industry in our country

Method used

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  • Mycoplasma bovis and application thereof
  • Mycoplasma bovis and application thereof
  • Mycoplasma bovis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the separation and identification of Mycoplasma bovis

[0020] 1.1 Disease data collection

[0021] The disease material came from a 2-month-old black-and-white cow that died of severe pneumonia and dyspnea in a large dairy farm in Jiyuan City, Henan Province in November 2016.

[0022] 1.2 Preparation of medium

[0023] PPLO liquid medium: PPLO broth powder 10.5g, glucose 2.5g, yeast powder 2.5g, dissolved in 440mL ultrapure water, sterilized at 115°C for 15min, added MEM medium 5mL, horse serum 50mL, penicillin 80,000 units, Sterile 10% arginine 10 mL and 1% (w / v) phenol red 500 μL. After the culture was sterilized at 115°C for 15 minutes, it was stored at 4°C for later use.

[0024] PPLO solid medium: PPLO liquid medium with 1.5% (w / v) agar powder. After the culture was sterilized at 115°C for 15 minutes, it was stored at 4°C for later use.

[0025] 1.3 Isolation and culture of mycoplasma

[0026] The collected diseased lung tissue was cut into sma...

Embodiment 2

[0046] Embodiment 2, the preparation of vaccine, safety and efficacy test

[0047] 2.1 Preparation of vaccine

[0048] The Mycoplasma bovis strain HNMB1 was inoculated into PPLO liquid medium and placed at 37°C, 5% CO 2 Cultivate in an incubator, collect the culture after 3 days, measure the concentration, and adjust the number of bacteria in the culture to 2×10 9 CFU / mL, add formaldehyde to a final concentration of 0.2%, inactivate at 37°C for 12h. The inactivated culture is added with an aluminum hydroxide adjuvant at a volume ratio of 1:1, and mixed to prepare an inactivated Mycoplasma bovis vaccine.

[0049] 2.2 Vaccine safety test:

[0050] Ten healthy black-and-white dairy cows aged 60 days were selected and divided into 2 groups with 5 cows in each group. Vaccine group: intramuscular injection of 10 mL of this vaccine in the neck; control group: intramuscular injection of 10 mL of sterilized normal saline in the neck. Observation for 30 days. During the observatio...

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Abstract

The invention discloses a Mycoplasma bovis and application thereof. The Mycoplasma bovis is Mycoplasma bovis HNMB1 and is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC NO:13295, the preservation data of November 28th, 2016 and the preservation address of No.3, Courtyard 1, Beichen West Road, Chaoyang District of Beijing. The strain HNMB1 is separated from a diary calf dead due to occurrence of typical respiratory symptoms, no other bacteria grow during separation on a PPLO solid culture medium, only mycoplasmas grow, and proliferation titer in a PPLO liquid culture medium is high and reaches up to more than 10<9>CFU/mL. The strain HNMB1 disclosed by the invention has stronger pathogenicity on the calf, results in pathogenesis and death of the calf and has good immunogenicity.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a strain of mycoplasma bovis and its application. Background technique [0002] Mycoplasma bovis mainly causes pneumonia, arthritis, mastitis, keratoconjunctivitis, reproductive tract inflammation, abortion and infertility in cattle. In 1961, Americans such as Hale first isolated Mycoplasma bovis from the milk of cows suffering from mastitis. In 1976, Mycoplasma bovis was described as the main cause of respiratory diseases. After that, Mycoplasma bovis and its related diseases spread rapidly to cattle in various countries and regions in the world, posing a great threat to the healthy development of the world's cattle industry. serious economic loss. Since 2008, the bovine respiratory infectious disease with fever, cough and runny nose as the main symptoms and necrotizing pneumonia as the main lesion has occurred and spread widely in many places in my country. It has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/35
CPCA61K39/0241A61K2039/521A61K2039/552A61K2039/55505C12N1/205C12R2001/35
Inventor 徐引弟李海利张青娴王治方郎利敏朱文豪焦文强王克领宋振宇索延乐
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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