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A strain of Mycoplasma gallisepticum and its application

A technology of mycoplasma gallisepticum and inactivated vaccines, applied in the field of molecular biology, can solve the problems of inability to inhibit the horizontal transmission of MG, and achieve good protective effects, strong pathogenicity, and good immunogenicity

Active Publication Date: 2020-12-18
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vaccine use, however, reduces MG emissions and reduces CRD symptoms, but does not inhibit horizontal transmission of MG

Method used

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  • A strain of Mycoplasma gallisepticum and its application
  • A strain of Mycoplasma gallisepticum and its application
  • A strain of Mycoplasma gallisepticum and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the separation and identification of Mycoplasma gallisepticum

[0020] 1.1 Disease data collection

[0021] The disease material came from the lungs of laying hens that died of sinusitis and dyspnea in a large laying hen farm in Xuchang City, Henan Province in April 2017.

[0022] 1.2 Preparation of medium

[0023] PPLO liquid medium: PPLO broth powder 10.5g, glucose 2.5g, yeast powder 2.5g, dissolved in 440mL ultrapure water, sterilized at 115°C for 15min, added MEM medium 5mL, horse serum 50mL, penicillin 80,000 units, Sterile mass fraction 10% arginine 10mL and 1% (w / v) phenol red 500μL. After the culture was sterilized at 115°C for 15 minutes, it was stored at 4°C for later use.

[0024] PPLO solid medium: PPLO liquid medium added with 1.5% (w / v) agar powder. After the culture was sterilized at 115°C for 15 minutes, it was stored at 4°C for later use.

[0025] 1.3 Isolation and culture of mycoplasma

[0026] Take an appropriate amount of diseased...

Embodiment 2

[0042] Embodiment 2, the preparation of vaccine, safety and efficacy test

[0043] 2.1 Preparation of vaccine

[0044] Inoculate the Mycoplasma gallisepticum strain HNMga1 into the PPLO liquid medium and place it at 37°C in 5% CO 2 Cultivate in an incubator, collect the culture after 3 days, measure the concentration, and adjust the number of bacteria of Mycoplasma gallisepticum in the culture to 2×10 7 CFU / mL, add 2mL formaldehyde solution (mass fraction: 37%) for every 1000mL culture, inactivate at 37°C for 12h to prepare the vaccine stock solution; then add aluminum hydroxide adjuvant (Sigma, F5881) equal to the volume of the vaccine stock solution , mixed to prepare Mycoplasma gallisepticum inactivated vaccine, the number of bacteria of Mycoplasma gallisepticum in the vaccine was about 1×10 7 CFU / mL.

[0045] 2.2 Vaccine safety test:

[0046] Twenty 45-day-old healthy chickens were selected, all of which were negative for Mycoplasma gallisepticum ELISA antibody. Divid...

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Abstract

The invention discloses a strain of Mycoplasma gallisepticum and its application. The Mycoplasma gallisepticum is Mycoplasma gallisepticum (Mycoplasma gallisepticum) HNMga1, preservation number: CGMCC NO: 13857, preservation date: May 26, 2017, preservation unit: General Microbiology Center of China Committee for Culture Collection of Microorganisms, deposit address: Beijing, China. Mycoplasma gallisepticum strain HNMga1 in the present invention is separated from the adult laying hen that occurs typical dyspnea and sinusitis, has stable biological characteristics, has strong pathogenicity to chickens, and can cause typical sinusitis and sinusitis in chickens. Difficulty, death symptoms, and good immunogenicity. The vaccine prepared by using the mycoplasma gallisepticum strain HNMga1 in the invention is safe and reliable, and has good protective effect on chronic respiratory diseases of chickens.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a strain of mycoplasma gallisepticum and its application. Background technique [0002] Mycoplasma gallisepticum (Mycoplasma gallisepticum, MG), also known as Mycoplasma gallisepticum, is the pathogen that causes chronic respiratory disease (CRD) or turkey infectious sinusitis in chickens and turkeys. It is characterized by cough, runny nose, airway rales, and mouth breathing. The pathogen exists in the respiratory tract, ovary, fallopian tube and semen of diseased chickens and infected chickens. The infected chicken embryos can be passed vertically to offspring, and cocks can spread the disease to the whole flock through mating. Once the flock is infected, it is difficult to completely eradicate it. Young chickens are more susceptible than adult chickens, and adult chickens often have no obvious symptoms. Stress factors and other respiratory pathogenic microorganisms...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K39/02A61P11/00A61P31/04C12R1/35
CPCA61K39/0241A61K2039/521A61K2039/55505C12N1/20C12N1/205C12R2001/35
Inventor 王治方徐引弟张青娴郎利敏焦文强张立宪李海利朱文豪游一王克领
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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