A Strain of Mycoplasma Hyopneumoniae and Its Application

A technology of Mycoplasma hyopneumoniae and an inactivated vaccine, which is applied in the field of Mycoplasma hyopneumoniae, can solve the problems of reduced mucociliary function, harm to the pig industry, difficult to cure, etc., and achieves stable biological characteristics, good immunogenicity, and good protection effect. Effect

Active Publication Date: 2020-12-25
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The attachment of Mhp in the respiratory tract can cause the accumulation of cilia and the lesion or necrosis of epithelial cells, and even destroy the mucosal layer of the respiratory tract, causing the cilia to shrink or fall off, which cannot effectively remove the debris and invading pathogens in the respiratory tract, resulting in the reduction of mucociliary function. , causing respiratory disease in pigs
The disease is widespread in all parts of the world. It is persistently infected and difficult to cure. At the same time, it causes serious harm to the pig industry due to the high treatment costs and reduced production performance of infected pigs.

Method used

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  • A Strain of Mycoplasma Hyopneumoniae and Its Application
  • A Strain of Mycoplasma Hyopneumoniae and Its Application
  • A Strain of Mycoplasma Hyopneumoniae and Its Application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the separation and identification of Mycoplasma hyopneumoniae

[0020] 1.1 Disease data collection

[0021] The disease material came from the lungs of a 2-month-old nursery pig that had severe pneumonia and dyspnea in a large pig farm in Puyang City, Henan Province in April 2017.

[0022] 1.2 Preparation of medium

[0023] PPLO liquid medium: PPLO broth powder 10.5g, glucose 2.5g, yeast powder 2.5g, dissolved in 440mL ultrapure water, sterilized at 115°C for 15min, added MEM medium 5mL, horse serum 50mL, penicillin 80,000 units, Sterile mass fraction 10% arginine 10mL and 1% (w / v) phenol red 500μL. After the culture was sterilized at 115°C for 15 minutes, it was stored at 4°C for later use.

[0024] PPLO solid medium: PPLO liquid medium added with 1.5% (w / v) agar powder. After the culture was sterilized at 115°C for 15 minutes, it was stored at 4°C for later use.

[0025] 1.3 Isolation and culture of mycoplasma

[0026] Take an appropriate amount of...

Embodiment 2

[0042] Embodiment 2, the preparation of vaccine, safety and efficacy test

[0043] 2.1 Preparation of vaccine

[0044] Inoculate the Mycoplasma hyopneumoniae strain HNMhy1 into PPLO liquid medium and place it at 37°C, 5% CO 2 Cultivate in an incubator, collect the culture after 3 days, measure the concentration, and adjust the number of bacteria of Mycoplasma hyopneumoniae in the culture to 2×10 9 CFU / mL, add 2mL formaldehyde solution (mass fraction: 37%) for every 1000mL culture, inactivate at 37°C for 12h to prepare the vaccine stock solution; then add aluminum hydroxide adjuvant (Sigma, F5881) equal to the volume of the vaccine stock solution , mixed to make Mycoplasma hyopneumoniae inactivated vaccine, the bacteria number of Mycoplasma hyopneumoniae in the vaccine is about 1×10 9 CFU / mL.

[0045] 2.2 Vaccine safety test:

[0046] Ten 15-day-old healthy piglets were selected, all of which were negative for Mycoplasma hyopneumoniae ELISA antibody. Divided into 2 groups,...

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Abstract

The invention discloses a strain of Mycoplasma hyopneumoniae and its application. The Mycoplasma hyopneumoniae is Mycoplasma hyopneumoniae (Mycoplasma bovis) HNMhy1, preservation number: CGMCC NO: 13858, preservation date: May 26, 2017, preservation unit: General Microbiology Center of China Committee for Culture Collection of Microorganisms, deposit address: Beijing, China. The mycoplasma hyopneumoniae strain HNMhy1 in the present invention is isolated from nursery pigs with typical dyspnea and lung consolidation, has stable biological characteristics, has strong pathogenicity to nursery pigs, and can cause typical panting in nursery pigs disease symptoms, and has good immunogenicity. The vaccine prepared by using the mycoplasma hyopneumoniae bacterial strain HNMhy1 in the invention is safe and reliable, and has a good protective effect on mycoplasma pneumonia in pigs.

Description

technical field [0001] The invention relates to the field of molecular biology technology, in particular to a strain of mycoplasma hyopneumoniae and its application. Background technique [0002] Mycoplasma hyopneumoniae (Mhp) is the main pathogen of Mycoplasma pneumonia of Swine (MPS). Mycoplasma swine pneumonia is a chronic, contagious infectious disease with high morbidity and low fatality rate. It mainly manifests as loss of appetite, fever, cough, wheezing, dyspnea and other symptoms. The diseased pigs grow slowly and the feed conversion rate Decreased, often induce the infection of other pathogens, especially immunosuppressive pathogens such as porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome, PRRSV), porcine circovirus type 2 (Porcine cirovirus 2, PCV-2), causing Induction of immunosuppression, often resulting in death from simultaneous secondary bacterial infection, is one of the leading swine diseases worldwide. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/35
CPCA61K39/0241A61K2039/521A61K2039/552C12N1/205C12R2001/35A61K2300/00
Inventor 徐引弟张青娴李海利郎利敏张立宪王治方朱文豪焦文强王克领游一
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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