The invention belongs to the field of microbial and animal
cell lines, and particularly relates to a
human breast cancer cell line MDA-MB-231 / 08-001 with spontaneous high
lung metastasis potentiality and an establishment method thereof. The cells of the
cell line are humanized cells that have 92 expression genes different from those of parent cells, higher in-vitro growth capacity, multiplication capacity, anti-invasion capacity, tumor forming property and
lung metastasis than the parent cells; and in 4 weeks after the cells are inoculated, the in-situ tumor volume of a scid mouse is 4,224.67+ / -1,038.95mm<3>, the weight is 2.09+ / -0.51g, the spontaneous
lung metastasis is 100 percent, and the number in a metastasis
lesion is 41 in each lung. The cell line and an established
animal model can simulate the whole process from the growth of the
tumor cells at implantation position to the formation of the metastasis
lesion at a
target organ, which is quite consistent with the clinical practical condition, and can be conveniently used for the research of
breast cancer lung metastasis mechanisms and related medicaments.