57results about How to "Stable biological properties" patented technology

A method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602 belongs to the technical field of microorganisms. In the invention, Bacillus coagulans CGMCC No.2602 is adopted, and under the condition of no oxygen supply, starchiness hydrolyzed sugar or dextrose is fermented by a semicontinuous intermittent fermentation way or an inter-sugar-compensating fermentation way to generate L-lactic acid with high optical purity. The invention has the advantages that the gemma property of the Bacillus coagulans is stable, and the L-lactic acid obtained by inoculating and fermenting the starchiness hydrolyzed sugar has high optical purity and rate of conversion of sugar and acid and short fermentation period; in addition, the semicontinuous intermittent fermentation way saves the time for preparing seeds by a continuous reladling and subculturing method, shortens the fermentation period, enhances the fermentation strength and obtains the L-lactic acid with both relatively high rate of conversion of sugar and acid and purity.

The invention relates to the field of vaccines of Haemophilus parasuis (HPs) in veterinary biological products and discloses a serotype 5 HPs vaccine strain. The class name of the vaccine strain is HPs. The strain number is XX0306. The vaccine strain has been collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:M2013095 and collection date being March 21, 2013. The serotype 5 HPs strain XX0306 has stronger pathogenicity toward swine and has good immunogenicity. The inactivated vaccines prepared from the vaccine strain are safe and reliable and have quite good protective effects on the swine challenging homological strains. The morbidity and mortality of the immunized swinery are obviously reduced. Either the single vaccine or the combined vaccine has the immune effects equivalent or superior to the immune effects of the existing commercialized vaccines and can effectively prevent prevalence of HPs.

The invention relates to the field of vaccines of Haemophilus parasuis (HPs) in veterinary biological products and discloses a serotype 4 HPs vaccine strain. The class name of the vaccine strain is HPs. The strain number is FS0307. The vaccine strain has been collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:M2013094 and collection date being March 21, 2013. The serotype 4 HPs strain FS0307 has stronger pathogenicity toward swine and has good immunogenicity. The inactivated vaccines prepared from the vaccine strain are safe and reliable and have quite good protective effects on the swine challenging homological strains. The morbidity and mortality of the immunized swinery are obviously reduced. Either the single vaccine or the combined vaccine has the immune effects equivalent or superior to the immune effects of the existing commercialized vaccines and can effectively prevent prevalence of HPs.

The invention belongs to the technical field of food processing methods, in particular to a special powder for mochi and a making method of the powder. The powder special for mochi is processed by mixing glutinous rice flour, modified corn starch, high maltose syrup, a compound improver and water by certain weight percent and carrying out the following steps: (1) mixing; (2) steaming; (3) mixing the high maltose syrup with the material; (4) taking out the steamed powder; (5) cooling the steamed powder; and (6) sterilizing and packaging the steamed powder. The invention has the following characteristics: by using the method in which the raw materials and the process are simultaneously improved, the problems that the traditional mochi powder is easy to harden and go bad, has poor taste and short shelf life and is hard to preserve are solved; and the mochi products made of the mochi powder have good elasticity, strong pliability, tender taste and more than 6 months of shelf life.

The invention discloses bacillus marinus capable of inducing disease resistance and stress tolerance of a plant. The bacillus marinus CT2628 (Bacillus sp.) is obtained from an ocean ecological environment through artificial induced mutation breeding and culture condition optimization; the proper artificial culture and fermentation condition is as follows: a proper fermentation culture solution of the CT2628 with the collection number of CGMCC No.8921 is prepared according to the following formula: 1-3g of a beef extract, 2-3g of peptone, 1-3g of yeast powder, 1-3g of glucose, 20-25g of NaCl, 0.3-0.5g of KCl, 1.9-2.5g of MgCl2.7H2O, 0.01-0.02g of FePO4 and 1000ml of water and has the pH value of 7.0-8.0. The invention discloses bacillus marinus capable of inducing disease resistance, salt resistance and cold resistance of the plant and application of the bacillus marinus to agriculture.

The invention relates to the field of vaccines of Haemophilus parasuis (HPs) in veterinary biological products and discloses application of a serotype 5 HPs vaccine strain. The class name of the vaccine strain is HPs. The strain number is XX0306. The vaccine strain has been collected in the China Center for Type Culture Collection, with collection number being CCTCC No:M2013095 and collection date being March 21, 2013. The serotype 5 HPs strain XX0306 has stronger pathogenicity toward swine and has good immunogenicity. The inactivated vaccines prepared from the vaccine strain are safe and reliable and have quite good protective effects on the swine challenging homological strains. The morbidity and mortality of the immunized swinery are obviously reduced. Either the single vaccine or the combined vaccine has the immune effects equivalent or superior to the immune effects of the existing commercialized vaccines and can effectively prevent prevalence of HPs.

The invention belongs to the technical field of biology, in particular relates to the technical field of biomedicine and in particular relates to a cell culture method for enabling human somatic cells to perform reversion differentiation for producing human blood derived auto-retina stem cells as well as a kit and application. The preparation method comprises the following steps: taking somatic cells as raw cells, sequentially culturing the raw cells through a cell culture solution A1, a cell culture solution A2 and a cell culture solution A3, thereby obtaining the blood derived auto-retina stem cells. The human blood cells serve as the raw cells, and the raw cells are subjected to reversion differentiation so as to produce the human blood derived auto-retina stem cells. A cell culture solution formula composed of small molecular substances is used, and the human blood cells are rapidly subjected to reversion differentiation so as to produce the human blood derived auto-retina stem cells under the conditions that the human somatic chromosome DNA sequence is not changed and any foreign gene or DNA fragment is not inserted. The production speed, yield and purity in the invention are obviously superior to those in the prior art.

The invention belongs to the field of biotechnology detection and particularly relates to a double-antibody sandwich ELISA antigen detection kit for Porcine deltacoronavirus (PDCoV) N protein. The kitcomprises a monoclonal antibody PDCoV-N-1A2 ELISA plate, positive and negative controls, an HRP labeled detection antibody PDCoV-N-5C5, a sample diluent, a color developing solution and a washing solution, wherein the antibodies PDCoV-N-1A2 and PDCoV-N-5C5 are secreted by hybridoma cell strains PDCoV-N-1A2 and PDCoV-N-1A2, respectively. The kit of the invention can specifically detect PDCoV N protein by the double-antibody sandwich ELSIA method established by using 1A2 as a capture antibody and using hybridization 5C5 (HRP coupled) as a detection antibody, and provides a new detection means for the clinical diagnosis and epidemiological investigation of PDCoV infection.

The invention discloses porcine mycoplasma pneumoniae and an application of the porcine mycoplasma pneumoniae. The porcine mycoplasma pneumoniae is mycoplasma bovis HNMhy1, has a collection number of CGMCC (China General Microbiological Culture Collection Center) NO: 13858 and is collected in China General Microbiological Culture Collection Center in Beijing, China on May 26, 2017. The porcine mycoplasma pneumoniae strain HNMhy1 is separated from a nursery pig having typical dyspnea and lung consolidation, has stable biological characteristics, and higher pathogenicity on the nursery pig, can cause a typical deep breathing symptom of the nursery pig and has good immunogenicity. A vaccine prepared from the porcine mycoplasma pneumoniae strain HNMhy1 is safe and reliable and has a better protection effect on the porcine primary atypical pneumonia.

The invention provides mycoplasma synoviae.A preservation number of the mycoplasma synoviae is CCTCC M 2016080.The mycoplasma synoviae has the advantages that a mycoplasma synoviae YBF-MS1 strain is stable in biological characteristics, high in toxicity and excellent in immunogenicity; oil adjuvant inactivated vaccine prepared from the mycoplasma synoviae is safe and reliable, is long in persistent period and has cross protection properties, high-level antibodies can be generated by the oil adjuvant inactivated vaccine, the post-immunization mycoplasma synoviae morbidity can be obviously reduced, and accordingly epidemic mycoplasma synoviae can be effectively prevented; mycoplasma synoviae vaccine is not available on market in China, the mycoplasma synoviae inactivated vaccine prepared from the mycoplasma synoviae YBF-MS1 strain is a key for preventing and controlling diseases, and market gaps can be filled.

The invention relates to the technical field of edible fungi, and in particular relates to a hypsizygus marmoreus strain and a culture method thereof. The hypsizygus marmoreus strain WZ-29 has a preservation number of GDMCC NO: 60276. Hypsizygus marmoreus grown from the hypsizygus marmoreus strain WZ-29 disclosed in the invention has extremely high homogeneity and excellent morphological characteristics, so that packaging and sale of the product and establishment of an edible fungus brand are facilitated. In addition, the strain has a short culture and cultivation period and stable biologicalcharacteristics, and greatly improves the production efficiency and stability of a plant; and yield per unit is high, every bottle is basically consistent when mass production is performed, and the strain has broad application prospects.

The invention discloses a haemophilus paragallinarum and application thereof. The haemophilus paragallinarum is haemophilus paragallinarum HNHpg1, the preservation number is CGMCC NO:13859, the preservation date is May 26th, 2017, the preservation place is China General Microbiological Culture Collection Center and the preservation address is Beijing, China. The haemophilus paragallinarum HNHpg1 is separated from secreta of sinus infraorbitalis of a laying hen suffering from typical respiratory symptoms and head and facial swelling, has stable biological characteristics, has stronger pathogenicity on chicks, can result in morbidity and death of the chicks and has good immunogenicity. A vaccine prepared by utilizing the haemophilus paragallinarum HNHpg1 disclosed by the invention is safe and reliable and has a better protective effect on chicken infectious rhinitis.

The invention belongs to veterinary biological products and more particularly relates to a method of preparing a porcine circovirus 2 type sensitive cell line, the cell line prepared therethrough, and an application of the cell line, and also provides a method of preparation of a high-titer porcine circovirus 2 type inactivated vaccine with the porcine circovirus 2 type sensitive cell line. The porcine circovirus 2 type sensitive cell line is uniform in shape, is stable in cell growth speed and biological characters, can generate high-titer 2-type porcine circovirus and can be used in researching and application of inactivated vaccines and relative diagnosis reagents of the 2-type porcine circovirus.

The invention discloses a method for culturing cord blood type embryonic stem cells as well as identification and application, relating to a method for culturing stem cells as well as identification and application. The culture method comprises: 1, collection of cord blood; 2, separation of the cord blood; 3, coating of a cord blood type embryonic stem cell culture dish; and 4, culture amplification of the cord blood type embryonic stem cells. The cord blood type embryonic stem cells are used for assisting recovery of injured lymphocytes. The formula of the culture medium adopts multiple cell factors to stably maintain the biological characteristic of cells and accelerate cell proliferation. The culture dish is coated by adopting laminin, and the laminin is beneficial to wall attachment of cells, so that the wall attachment time of the cells is effectively shortened, the adhesion degree of the cells to the bottom of the dish is improved and the cells are attached to the wall more firmly.

The invention belongs to the field of microbial and animal cell lines, and particularly relates to a human breast cancer cell line MDA-MB-231/08-001 with spontaneous high lung metastasis potentiality and an establishment method thereof. The cells of the cell line are humanized cells that have 92 expression genes different from those of parent cells, higher in-vitro growth capacity, multiplication capacity, anti-invasion capacity, tumor forming property and lung metastasis than the parent cells; and in 4 weeks after the cells are inoculated, the in-situ tumor volume of a scid mouse is 4,224.67+/-1,038.95mm<3>, the weight is 2.09+/-0.51g, the spontaneous lung metastasis is 100 percent, and the number in a metastasis lesion is 41 in each lung. The cell line and an established animal model can simulate the whole process from the growth of the tumor cells at implantation position to the formation of the metastasis lesion at a target organ, which is quite consistent with the clinical practical condition, and can be conveniently used for the research of breast cancer lung metastasis mechanisms and related medicaments.