Spontaneous lymphocyte highly metastatic human ovarian sarcomatoid carcinoma cell line

A high-metastasis technology of ovarian cancer cells, applied in the fields of biotechnology and microbial animal cell lines, can solve the problems of not fully reflecting the real clinical situation, and achieve the effect of shortening the experimental period and facilitating observation

Inactive Publication Date: 2010-09-29
THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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Problems solved by technology

Clinical practice shows that ovarian cancer rarely metastasizes to the lung, and the researchers believe that the highly metastatic strains found from lung metastases may not fully reflect the real clinical situation

Method used

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  • Spontaneous lymphocyte highly metastatic human ovarian sarcomatoid carcinoma cell line
  • Spontaneous lymphocyte highly metastatic human ovarian sarcomatoid carcinoma cell line
  • Spontaneous lymphocyte highly metastatic human ovarian sarcomatoid carcinoma cell line

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Embodiment 1

[0021] (1) Cell culture

[0022] The cells involved in the present invention are all cultured in McCoy's5A cell culture medium containing 10% fetal calf serum (FCS). The culture temperature is 37° C., the gas environment is 5% CO2 / 95% air, and the humidity is saturated humidity. When subcultured, the cells were digested with 0.25% trypsin, and subcultured at a ratio of 1:3.

[0023] (2) Screening and establishment of lymphatic highly metastatic cells

[0024] 1. The first round of screening of lymphatic high metastasis cells

[0025] ①Digest the SKOV-3 cells in the logarithmic phase of growth with 0.25% trypsin to make a single cell suspension;

[0026] ②Under sterile conditions, use a 1ml syringe to inoculate tumor cells into the abdominal cavity of 6-week-old BALB / c female nude mice, with an inoculation volume of 5×10 6 Cells / 1ml / one, five in total;

[0027] ③After 4 weeks of routine feeding, the nude mice were killed by decapitation, and soaked in 75% alcohol for 5 min...

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Abstract

The invention belongs to the fields of biotechnology and microorganism animal cell lines, and in particular relates to a spontaneous lymphocyte highly metastatic human ovarian sarcomatoid carcinoma cell line, and an establishment method and application thereof. In the invention, the human ovarian sarcomatoid carcinoma cell line SKOV-3 (ATCC code: HTB-77) is taken as a parent cell; and the spontaneous lymphocyte highly metastatic human ovarian sarcomatoid carcinoma cell line SKOV-3 / LN403 is obtained by separating and through strategies, such as BALB / c nude mouse intraperitoneal injection, retroperitoneal lymph node continuous screening, amplification in vitro DNA, passage and the like, and the preservation number is CGMCC No.2940. The cell line and an animal model established thereby can simulate the actual clinical situation of a patient with ovarian cancer so as to provide an ideal experiment tool for researching a biological behavior and a regulation and control mechanism for ovarian cancer lymph node metastasis, and screening clinical anti-metastasis medicaments.

Description

technical field [0001] The invention belongs to the fields of biotechnology and microbial animal cell lines, and specifically relates to a spontaneous lymphatic highly metastatic human ovarian cancer cell line and its establishment method and application. Background technique [0002] Studies have reported that the mortality rate of ovarian cancer ranks first among malignant tumors of the female reproductive system, and high recurrence and metastasis rates and chemotherapy resistance have become two major characteristics that affect prognosis. Lymphatic metastasis is the main way of early dissemination of ovarian cancer, and it is an important factor that causes postoperative recurrence of ovarian cancer and affects the prognosis. Lymph node metastasis occurs in 48% to 70% of patients who die from ovarian cancer (Pereira A, Magrina JF, Rey V, et al. ). The metastasis rate of retroperitoneal lymph nodes in patients with advanced stage is 55%-75% (Di Re F, Baiocchi G. Value ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12Q1/18C12R1/91
Inventor 康玉徐从剑罗建明程明军黄以萍丛青武欣张晓燕吴志勇黄宇婷张雯碧王宜生
Owner THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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