865 results about "BALB/c" patented technology

The invention relates to BALB / c mice that are deficient in the programmed cell death-1 receptor (PD-1), a screening method for autoimmune disease medicine using these mice, an IgG self-reactive antibody that the mice produce, a protein that specifically reacts to the antibody and is produced in heart, and a diagnostic method for dilated cardiomyopathy using the protein. Because PD-1 deficient BALB / c mice spontaneously develop autoimmune disease, and specifically dilated cardiomyopathy, they are useful for screening for medicines against these diseases.

The invention discloses a variable region of heavy and light chains of an anti-human IL-13R alpha 2 single clone antibody. The invention uses recombinant human IL-13R alpha 2 to immunize a BALB / c rat to prepare a group of rate anti-human IL-13R alpha 2 single clone antibody and screen anti-human IL-13R alpha 2 single clone antibody FMMU-IL-13R alpha 2-7 with high affinity. The variable region genes of heavy and light chains of the single clone antibody are cloned to obtain the sequences of genes and amino acid in the variable region of the heavy and light chains of the single clone antibody, and confirm the uniqueness of the gene and protein sequences. The amino acid sequence of the variable region and the gene sequences encoding the variable zones have great potential application value in single chain antibody, chimeric antibody, humanized antibody or vaccine for treating malignancy with human IL-13R alpha 2 as the target.

The invention discloses a double-antibody sandwich method for detecting salmonella typhimurium in food based on monoclonal antibodies, belonging to the technical field of immunoassay. Salmonella typhimurium ATCC13311 and smooth salmonella typhimurium LPS are adopted for mixed immunity of a 7-week BALB / c mouse, 10 LPS monoclonal antibodies are obtained by immunity, fusion and screening, horse radish peroxidases (HRP) are labeled respectively, and the salmonella typhimurium is paired two by two. A sandwich enzyme-linked immuno sorbent assay (ELISA) method is established by taking 6E2 CGMCC No.7206 monoclonal antibodies as coated antibodies and enzyme-labeled antibodies and by taking the salmonella typhimurium as standards, and the LOD is 500cfu / mL. The sandwich method, established by using the monoclonal antibodies which are highly uniform in physicochemical property and high in specificity and can be prepared on a large scale, is high in sensitivity and low in cost; the salmonella typhimurium is not in cross reaction with salmonella enteritidis, salmonella arizonae, E.coli, E.coliO157:H7, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes; a quick and efficient analysis way is provided for detection of the salmonella typhimurium in the food.

The invention discloses a method for quantitatively detecting allergen alpha-lactalbumin based on quantum dot fluorescence and application of the allergen alpha-lactalbumin. The method comprises the following steps of: preparing a monoclonal antibody from alpha-lactalbumin as active immunization BALB / c mice; carrying out conjugation labeling on the alpha-lactalbumin monoclonal antibody by using a fluorescent quantum dot; forming an immunofluorescence complex by adopting a competitive immunosorbent assay; then detecting a fluorescent signal under a full-wavelength multifunctional ELIASA (Enzyme-Linked Immunosorbent Assay Apparatus); and quantitatively detecting the allergen alpha-lactalbumin in the food by establishing a standard curve. The method constructed by the invention can be widely applied to detection of relevant allergens in various powders and liquid milk products, has the characteristics of quickness, accuracy, high sensitivity, favorable repeatability, excellent specificity and the like, provides an effective means for high-throughput detection of relevant allergens in various foods as well as has a favorable popularization and application prospect.

The invention relates to an enrofloxacin monoclonal antibody and application, relates to hybridoma strains thereof, and belongs to the technical field of immunochemistry. The enrofloxacin monoclonal antibody is generated by mouse hybridoma strains 6A4 and 8E6. The preparation method comprises the following steps that: enrofloxacin and carrier proteins BSA, HAS and OVA are coupled by a carbodiimide method to synthesize artificial immunogens EnR-BSA, EnR-HSA and coatingen EnR-OVA; a Balb / c mouse is immunized by the synthesized artificial immunogens EnR-BSA and EnR-HSA; a spleen cell of the immunized mouse is extracted to be fused with a SP2 / O myeloma cell and coated by the coatingen EnR-OVA; indirect ELISA method and indirect competition ELISA method are established to screen the hybridoma strains which can stably secrete specific antibody; the obtained cell strain immunized Balb / c mouse is used to prepare ascites; the ascites is purified by a caprylic acid-ammonium method and an ion exchange method; and valences of antibodies of two purified cell strains reach over 1.024*10 and 1.28*10. The monoclonal antibody has strong specificity, can be applied to preparation of enrofloxacin residue inspection kit and aerosol test strip, and can sensibly and quickly inspect the enrofloxacin residue.

The invention relates to a preparation method of a benzothiostrobin antigen and antibody and an application thereof, belonging to the technical field of immunochemical analysis. The chemical name of benzothiostrobin is 2-[[(5-methoxy-2-benzothiazole)-thiomethyl]-alpha-(E)-methoxymethylene]methyl phenylacetate. Under an alkaline condition, carboxylic ester in the benzothiostrobin structure is hydrolyzed to synthesize an artificial hapten of which the chemical name is 2-[[(5-methoxy-2-benzothiazole)-thiomethyl]-alpha-(E)-methoxymethylene]phenylacetic acid; the artificial hapten is coupled with bovine serum albumin and ovalbumin respectively to prepared an artificial antigen. The BALB / c female mouse is immunized by the artificial antigen to obtain a specific monoclonal antibody of benzothiostrobin. The antibody does not experience a cross reaction with other compounds. An enzyme linked immunosorbent assay method established by use of the antibody can be applied to quick, sensitive, convenient and cheap detection of benzothiostrobin residues in environment and agricultural products.

The invention discloses a specific double antibody sandwich method for detecting salmonella in food based on a monoclonal antibody, and belongs to the field of immunoassay. The specific double antibody sandwich method comprises the following steps: immunizing a 7-week-old BALB / c mouse by using smooth salmonella typhimurium LPS-coupled bovine serum albumin according to a sodium periodate method, and carrying out immunization, fusion and screening to obtain 12 monoclonal antibodies for specifically identifying salmonella LPS; marking the 12 monoclonal antibodies with horseradish peroxidase HRP respectively, and pairing the 12 monoclonal antibodies with salmonella paratyphi bacteria; using 8G3 as a coated antibody and 8G3-HRP as a detection antibody to establish the salmonella detecting specific double antibody sandwich method, so that salmonella paratyphi with the LOD of 1000000 CFU / mL can be detected. The specific double antibody sandwich method has cross reactions with all salmonella bacteria, but does not have cross sections with E.coli, E.coli O157:H7, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes, so that an overall, reliable, fast and efficient analysis method is provided for detection of the salmonella in the food.