Preparing method for paclobutrazol monoclonal antibodies

Abstract

The invention discloses a preparing method for paclobutrazol monoclonal antibodies. The preparing method comprises the main steps as follows: (1) synthesizing artificial hapten paclobutrazol hemisuccinates by a microwave solvent free method; (2) performing coupling to obtain artificial antigens of the paclobutrazol by using the paclobutrazol hemisuccinates as raw materials; (3) performing the immune treatment on Balb / c mice by using the synthesized artificial antigens; (4) selecting the mice with optimal serum valence and optimal specificity, and mixing spleen cells and myeloma cells of the mice together in an external manner; (5) culturing and screening the fused cells by using the selective culture medium, and further cloning, propagating and storing by freezing; (7) injecting the expanded cell strains into the abdominal cavities of the mice to generate a lot of abdominal dropsy; (8) purifying the paclobutrazol-resistant monoclonal antibodies in the abdominal dropsy by a caprylic acid-saturated ammonium sulfate method. The prepared paclobutrazol-resistant monoclonal antibodies have high sensitivity and strong specificity, and can be further applied to the construction of technologies of immune sensors, colloidal gold immune chromatographic methods and the like.

Description

[0001] technical field

[0002] The invention relates to a preparation method of paclobutrazol monoclonal antibody, belonging to the technical field of monoclonal antibody preparation. Background technique

[0003] Paclobutrazol is a growth-inhibiting plant growth regulator. It has the effects of delaying plant growth, inhibiting stem elongation, shortening internodes, promoting flower bud differentiation, increasing plant stress resistance, and increasing yield. Therefore, it is widely used in rice, Wheat, peanuts, fruit trees, tobacco, rapeseed, soybeans, flowers, lawns, etc. as (plants). With the continuous expansion of the use of plant growth regulators, the chronic and long-term effects of their residues on the environment and human health have increasingly aroused people's attention and concern. For this reason, the restrictions on the residues of plant growth regulators are becoming more and more strict. my country has stricter limit standards for the residues of pac...

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