Compound probiotics and drug for treating ulcerative colitis and preparation method thereof

A technology of ulcerative colitis and compound probiotics, applied in the field of medicine, can solve the problems of UC, toxicity and carcinogenicity, drug resistance and other problems in patients who cannot be cured

Active Publication Date: 2016-02-24
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, UC cannot be cured, and the symptoms are not cured; secondly, the side effects are large, and it is easy to cause headaches, nausea, and abnormalities in the digestive system and blood system of the body; secondly, the potential toxicity and carcinogenicity; lastly, antibiotics (if used The most widespread SASP) can easily lead to drug resistance in the body

Method used

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  • Compound probiotics and drug for treating ulcerative colitis and preparation method thereof
  • Compound probiotics and drug for treating ulcerative colitis and preparation method thereof
  • Compound probiotics and drug for treating ulcerative colitis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0060] (3) Preparation of medicines for treating ulcerative colitis

[0061] Take out the slime tubes of Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 respectively dissolved in 0.1mL sterile PBS solution, mix them, and then prepare the medicine for this experiment.

[0062] (4) Oral gavage preparation of other groups of mice

[0063] Drugs for intragastric administration of antibiotic group mice: the intragastric dosage of antibiotics is 9.2mg / 20g (sulfalazine powder weight / mouse weight); PBS solution, mixed, and then stored at 4°C for gavage.

[0064] Oral administration of probiotics to mice in the control group: Lactobacillus acidophilus and Lactobacillus plantarum on the market were counted and centrifuged as above, and Lactobacillus acidophilus and Lactobacillus plantarum at the beginning of the stable period were respectively taken 1×10 9 CFU, the two were mixed, then dissolved in 0.3mL of sterile PBS solution, mixed, and then stored at 4°C...

Embodiment 1

[0084] A medicine for treating ulcerative colitis by using compound probiotics, said compound probiotics comprising Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961, the number of cells of said Lactobacillus acidophilus CGMCC10436 accounts for 10% of the total quantity, and the total dosage of Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 is 1×10 11 CFU / kg, and both are in the form of live bacteria. The living bacteria cells are respectively centrifuged and collected bacteria sludge from liquid cultures of Lactobacillus in the initial stage of stability.

[0085] The preparation method of the above-mentioned medicine for treating ulcerative colitis by using compound probiotics comprises the following steps:

[0086] (1) respectively culture Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 in MRS medium for 12 hours;

[0087] (2) Take 40 parts of Lactobacillus acidophilus CGMCC10436 bacterium liq...

Embodiment 2

[0090] A medicine for treating ulcerative colitis by using compound probiotics, said compound probiotics comprising Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961, the number of cells of said Lactobacillus acidophilus CGMCC10436 accounts for 50% of the total quantity, and the total dosage of Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 is 1×10 11 CFU / kg, and both are in the form of live bacteria. The living bacteria cells are respectively centrifuged and collected bacteria sludge from liquid cultures of Lactobacillus in the initial stage of stability.

[0091] The preparation method of the above-mentioned medicine for treating ulcerative colitis by using compound probiotics comprises the following steps:

[0092] (1) respectively culture Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961 in MRS medium for 16 hours;

[0093] (2) Take 60 parts of Lactobacillus acidophilus CGMCC10436 bacterium sol...

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Abstract

The invention discloses compound probiotics and drug for treating ulcerative colitis and a preparation method thereof. The compound probiotics comprises Lactobacillus acidophilus CGMCC10436 and Lactobacillus plantarum CGMCC9961, and the drug for treating the ulcerative colitis adopts the two strains of the probiotics in a combined mode. According to the compound probiotics and drug for treating the ulcerative colitis and the preparation method thereof, Balb / c mice serve as test objects to establish an ulcerative colitis model, and the effect of the drug for resisting the ulcerative colitis is researched by starting from the angles such as apparent states and pathological conditions of the tested mice. It is shown through the test results that the drug has a good effect on resisting the ulcerative colitis, relapse of symptoms does not occur in a short period, the intestinal mucosa damage caused by the ulcerative colitis can be well repaired, and the drug for treating the ulcerative colitis can be widely applied to the field of preclinical medicine.

Description

technical field [0001] The invention relates to a compound probiotic for treating ulcerative colitis, a medicine and a preparation method thereof, that is, a medicine used in combination with two strains of probiotics, and belongs to the technical field of medicine. Background technique [0002] Ulcerative colitis (ulcerative colitis, UC) is a major type of inflammatory bowel disease (inflammatory bowel disease, IBD), is a chronic non-specific intestinal inflammation of unknown cause. Statistics show that the annual prevalence of UC in the United States is 200 / 100,000 people, and the cost of treating UC is about 100 to 150 million US dollars. As of 2012, the total number of UC patients is 2.5 times that of 2000, and the recurrence rate is 72%; In recent years, the number of patients with UC in China has shown an obvious upward trend. Ulcerative colitis lesions are mainly located in the colonic mucosa and submucosa; the clinical manifestations are diarrhea, mucus pus and blo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P1/00A61P1/04C12N1/20C12R1/25C12R1/23
CPCA61K35/747C12N1/205C12R2001/23C12R2001/25A61K2300/00
Inventor 霍贵成徐敏边鑫
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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