Melamine and carrier protein couplet product, preparation method and uses of melamine antibody

A technology of melamine and carrier protein, applied in chemical instruments and methods, ovalbumin, albumin peptides, etc., can solve the problems of cumbersome operation, expensive instruments, time-consuming and laborious, etc., and achieve the effect of simple and convenient operation and simple and effective method.
CN101429243AInactive Publication Date: 2009-05-13ZHEJIANG UNIV
0 Cites 17 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2009-05-13
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention discloses a preparation method and application of a product obtained by coupling melamine with carrier protein, as well as a melamine antibody. The product obtained by coupling the carrier protein with the melamine is used as artificial antigen and applied to an immunological method for melamine detection. The preparation method comprises the following steps of immunizing animals with the coupled product so as to prepare an antibody used for melamine detection, fusing BALB / C mouse spleen cells immunized with the coupled product and SP2 / 0 mouse myeloma cells, obtaining hybridoma capable of stably transferring culture and secreting anti-melamine specific monoclonal antibodies by screening positive hybridoma and cloning cells, and preparing an ascites monoclonal antibody. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the melamine, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling melamine with carrier protein, as well as the melamine antibody provides service for the rapid detection of melamine-type residue in foods.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of immunochemistry and biology, and in particular relates to a preparation method and application of a coupling product of melamine and carrier protein and melamine antibody. Background technique

[0002] Melamine is referred to as triamine for short, and its scientific name is triaminotriazine, and its aliases are melamine, cyanuramide, and trimeramide. Molecular formula: C3N6H6, C3N3(NH2)3. Molecular weight: 126.12, is an important nitrogen heterocycle organic chemical raw material. Melamine is weakly alkaline and can react with various acids to form melamine salts. In strong acid or strong lye, melamine is hydrolyzed, and the amino groups are gradually replaced by hydroxyl groups to generate ammeline, ammeline and cyanuric acid. Melamine reacts with aldehydes to form addition compounds. Melamine is reacted with aldehydes to form a resin. Melamine resin is a versatile material that is fire and heat resi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More